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The map label for this gene is citH [H]

Identifier: 146341141

GI number: 146341141

Start: 4413710

End: 4414990

Strand: Direct

Name: citH [H]

Synonym: BRADO4212

Alternate gene names: 146341141

Gene position: 4413710-4414990 (Clockwise)

Preceding gene: 146341139

Following gene: 146341148

Centisome position: 59.19

GC content: 66.28

Gene sequence:

>1281_bases
ATGCAGCCGTCACCAACCGATGCGATGCGTCGCCGTGTCGTGATCTCCTCGACCATCGGCAATGCGCTCGAATGGTTCGA
CTTCACCGTCTTCGGGCTGTTCGCCGCGACCATCAGCAAGCTGTTCTTTCCCGCCGACGATCCGCTGGCATCGCTGCTCT
CGACATTTGCGGTATTTGGCCTCGCCTTCGTCGCGCGCCCGCTCGGCGGACTGGTGTTCGGCCTCTACGCCGACCGCTAC
GGGCGCAAGGCGGCGCTGGTCGTGATGATCACGATGATGGCGATCGGCACCGGCCTGATCGGCGTGCTGCCGACCTTCGC
ATCGATCGGCATTGCCGCGCCGCTCTTGCTGCTGCTCGCGCGTCTGATCCAGGGGTTCTCGGCCGGCGGCGAGTTCGGCT
CGGCGAGCGCCATGCTGATCGAGTTCGCTCCGCCAGGGCGCAAGGGGTTCTATGGCGCTTTCCAGACCGTGTCGCAGGCG
CTCGCCTTCGCGCTCGGCGCAATGATGGCGGTCACGCTCAGCAAGACGCTGTCGCCCGCGGATTTCGCCAGCTGGGGCTG
GCGGGTGCCGTTCATCCTGGGAATCCTGATCGGGCCCGTCGGCTGGTATCTGCGCCGCCGATGCGATGAATCGCCTGAAT
TCCGGACCCATATGGCGGAACGTGCAGCAAGAGCCACGCCTGCAAGAAAGACCCCGCTCGGGCAGCTGTTCGCGGAGCAT
CCGCGCGAGCTCATGGCCTCGTTCGGCCTGATCGCGGCCGGCACCGCGATCTACTATGTCGGCGGTGTGTTCCTGCCGGC
CTACGCCGCGAGCGAGCTCAAGCTGCCGCTGGCCGACGCGCAGCTCGGGCTGCTCGCGGTCAGCCTCAGCAACATTCCGC
TGGGTCTCGCGTCGGGCATGCTCTCTGATCGCATCGGGCGGCGTGGCGTCATCTTGCCGGCGCTGCTGCTCTATCCCATC
CTCTACGTGGTGTTGTTCAGGCAGCTGGTGGCGGAGCCGACCACCGCGCATCTGTGGCAGCTCCAGTCCGTCGGCCTGAT
CCTCGGCGTGCTCGGCGGCGCGGTGCCGGCGTTCATGACCGAGATCTTTCCTGTCGGCGTGCGCGCGACCGGGGCGTCGC
TGATGTACAATCTCGGCGTGATGCTGTTCGGTGGCCTGGCGCCGTTCATCAATACCTGGATGGTTCAAGCCACCGGCGAC
AAGTCCGCGCCGGTCTACTACATTCTCGCCGCCGCCGCGGTCGGCCTGCTCGGGATGGTCGTGTACCGCAGGAGAACATA
A

Upstream 100 bases:

>100_bases
TGAACTCGGTTCGTTGCGGGCACGCAGCCTGTCGCGGCATGGCTGCGTGCAGCCGGGATGACGCTCGGGAGACCACGTGA
CTCCAACGGACGATTGAGCC

Downstream 100 bases:

>100_bases
GCGCGTCAGCGCGTGCGCGGCTGCGATCGCAGCGCGCGCAGCTCGCGCGCGAGATTGGCTCGCGCCCGCTCGTCGAGCGC
GGCGGCAAACGTCGCATCCG

Product: major facilitator superfamily citrate transporter permease

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MQPSPTDAMRRRVVISSTIGNALEWFDFTVFGLFAATISKLFFPADDPLASLLSTFAVFGLAFVARPLGGLVFGLYADRY
GRKAALVVMITMMAIGTGLIGVLPTFASIGIAAPLLLLLARLIQGFSAGGEFGSASAMLIEFAPPGRKGFYGAFQTVSQA
LAFALGAMMAVTLSKTLSPADFASWGWRVPFILGILIGPVGWYLRRRCDESPEFRTHMAERAARATPARKTPLGQLFAEH
PRELMASFGLIAAGTAIYYVGGVFLPAYAASELKLPLADAQLGLLAVSLSNIPLGLASGMLSDRIGRRGVILPALLLYPI
LYVVLFRQLVAEPTTAHLWQLQSVGLILGVLGGAVPAFMTEIFPVGVRATGASLMYNLGVMLFGGLAPFINTWMVQATGD
KSAPVYYILAAAAVGLLGMVVYRRRT

Sequences:

>Translated_426_residues
MQPSPTDAMRRRVVISSTIGNALEWFDFTVFGLFAATISKLFFPADDPLASLLSTFAVFGLAFVARPLGGLVFGLYADRY
GRKAALVVMITMMAIGTGLIGVLPTFASIGIAAPLLLLLARLIQGFSAGGEFGSASAMLIEFAPPGRKGFYGAFQTVSQA
LAFALGAMMAVTLSKTLSPADFASWGWRVPFILGILIGPVGWYLRRRCDESPEFRTHMAERAARATPARKTPLGQLFAEH
PRELMASFGLIAAGTAIYYVGGVFLPAYAASELKLPLADAQLGLLAVSLSNIPLGLASGMLSDRIGRRGVILPALLLYPI
LYVVLFRQLVAEPTTAHLWQLQSVGLILGVLGGAVPAFMTEIFPVGVRATGASLMYNLGVMLFGGLAPFINTWMVQATGD
KSAPVYYILAAAAVGLLGMVVYRRRT
>Mature_426_residues
MQPSPTDAMRRRVVISSTIGNALEWFDFTVFGLFAATISKLFFPADDPLASLLSTFAVFGLAFVARPLGGLVFGLYADRY
GRKAALVVMITMMAIGTGLIGVLPTFASIGIAAPLLLLLARLIQGFSAGGEFGSASAMLIEFAPPGRKGFYGAFQTVSQA
LAFALGAMMAVTLSKTLSPADFASWGWRVPFILGILIGPVGWYLRRRCDESPEFRTHMAERAARATPARKTPLGQLFAEH
PRELMASFGLIAAGTAIYYVGGVFLPAYAASELKLPLADAQLGLLAVSLSNIPLGLASGMLSDRIGRRGVILPALLLYPI
LYVVLFRQLVAEPTTAHLWQLQSVGLILGVLGGAVPAFMTEIFPVGVRATGASLMYNLGVMLFGGLAPFINTWMVQATGD
KSAPVYYILAAAAVGLLGMVVYRRRT

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=425, Percent_Identity=33.1764705882353, Blast_Score=254, Evalue=9e-69,
Organism=Escherichia coli, GI1788292, Length=421, Percent_Identity=30.8788598574822, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1789941, Length=414, Percent_Identity=36.4734299516908, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1788942, Length=435, Percent_Identity=32.8735632183908, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI87082404, Length=402, Percent_Identity=27.1144278606965, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87082231, Length=213, Percent_Identity=28.6384976525822, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45352; Mature: 45352

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPSPTDAMRRRVVISSTIGNALEWFDFTVFGLFAATISKLFFPADDPLASLLSTFAVFG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LAFVARPLGGLVFGLYADRYGRKAALVVMITMMAIGTGLIGVLPTFASIGIAAPLLLLLA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLIQGFSAGGEFGSASAMLIEFAPPGRKGFYGAFQTVSQALAFALGAMMAVTLSKTLSPA
HHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DFASWGWRVPFILGILIGPVGWYLRRRCDESPEFRTHMAERAARATPARKTPLGQLFAEH
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
PRELMASFGLIAAGTAIYYVGGVFLPAYAASELKLPLADAQLGLLAVSLSNIPLGLASGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEECCCCCHHHHHHH
LSDRIGRRGVILPALLLYPILYVVLFRQLVAEPTTAHLWQLQSVGLILGVLGGAVPAFMT
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EIFPVGVRATGASLMYNLGVMLFGGLAPFINTWMVQATGDKSAPVYYILAAAAVGLLGMV
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHH
VYRRRT
HHHCCC
>Mature Secondary Structure
MQPSPTDAMRRRVVISSTIGNALEWFDFTVFGLFAATISKLFFPADDPLASLLSTFAVFG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LAFVARPLGGLVFGLYADRYGRKAALVVMITMMAIGTGLIGVLPTFASIGIAAPLLLLLA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLIQGFSAGGEFGSASAMLIEFAPPGRKGFYGAFQTVSQALAFALGAMMAVTLSKTLSPA
HHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DFASWGWRVPFILGILIGPVGWYLRRRCDESPEFRTHMAERAARATPARKTPLGQLFAEH
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
PRELMASFGLIAAGTAIYYVGGVFLPAYAASELKLPLADAQLGLLAVSLSNIPLGLASGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEECCCCCHHHHHHH
LSDRIGRRGVILPALLLYPILYVVLFRQLVAEPTTAHLWQLQSVGLILGVLGGAVPAFMT
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EIFPVGVRATGASLMYNLGVMLFGGLAPFINTWMVQATGDKSAPVYYILAAAAVGLLGMV
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHH
VYRRRT
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]