BacMap

Definition	Bradyrhizobium sp. ORS278 chromosome, complete genome.
Accession	NC_009445
Length	7,456,587

Click here to switch to the map view.

The map label for this gene is fadB1 [H]

Identifier: 146341148

GI number: 146341148

Start: 4419567

End: 4420400

Strand: Direct

Name: fadB1 [H]

Synonym: BRADO4219

Alternate gene names: 146341148

Gene position: 4419567-4420400 (Clockwise)

Preceding gene: 146341141

Following gene: 146341149

Centisome position: 59.27

GC content: 67.99

Gene sequence:

>834_bases
ATGGAAATCGGAATGTCCGCCCCTGCCCTCGCCGATGCGACCGCCACGCCGATCCTGCTGCGCGAGATGGCCGGCTCCGT
CGCGGTGCTGACGCTGAACCGGCCCGCCCAGCGCAACAGCCTCTCGGAGTCGCTGATCGCCGAGTTGCATCAGATGCTCG
ACGAGATCGGCGCCGACAAGGCGGTGCGCGCCGTCGTGATCGCCGCCAATGGCCCGGCCTTCTGCGCCGGCCACGATCTG
AAAGAGCTCACCGCGCGCCGAGCCGACGCCGATCGCGGCCGCGCCTTCTTCGCCAGGCTGATGAACGCCTGCAGCGCGAT
GATGCAGGCGATCGTCCACATGCCGAAGCCCGTCGTCGCCTCCGTGCAGGGCATCGCCACCGCGGCAGGCTGCCAGCTGG
TCGCGAGCTGCGATCTGGCCGTGGCCTCCGAGCACGCCTCCTTCGCCACGCCCGGCGTCGACATCGGCCTGTTCTGCTCG
ACCCCGATGGTGGCGCTGTCGCGCAACATCACACGCAAGCAGGCCATGGAGATGCTGCTGACCGGCGAGCCGGTTCCGGC
GCCGCGCGCCCGCGAGATCGGTCTCGTCAACCGCGTGGTAACGCCCGGCACGGAACGCGAGACGGCGATTGCGCTGGCAC
AACAAGTCGCGCGCAAATCCGCGCATACGATCAAGCTCGGCAAGGAGGCATTCTACCGCCAGGCCGAGATGAGCCTTGCC
GACGCCTATCGTTATGCGGCAGAGGTTATGACCGAAAACATGCTCGCCCGCGACGCGGAAGAAGGCATCGGCGCCTTCAT
CGAGAAGCGGACGCCGACCTGGCGGGATGAGTGA

Upstream 100 bases:

>100_bases
CAGGAACTCCTCCAGCTCCGGAATGGTCATCTTGGCCGTCATGTCTCCCCCTGCTCTGGTTGTTGGCGATCTGTTACATT
AGCTGCAACCACACGCCCAT

Downstream 100 bases:

>100_bases
CCGCTCTCCTCTCTCTCCACGTCATTGCGAGCGAAGCGAAGCAATCCAGAGTCCCACCCGAGCCCCTGGATTGCTTCGCT
TCGCTCGCAATGACGCAAAC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MEIGMSAPALADATATPILLREMAGSVAVLTLNRPAQRNSLSESLIAELHQMLDEIGADKAVRAVVIAANGPAFCAGHDL
KELTARRADADRGRAFFARLMNACSAMMQAIVHMPKPVVASVQGIATAAGCQLVASCDLAVASEHASFATPGVDIGLFCS
TPMVALSRNITRKQAMEMLLTGEPVPAPRAREIGLVNRVVTPGTERETAIALAQQVARKSAHTIKLGKEAFYRQAEMSLA
DAYRYAAEVMTENMLARDAEEGIGAFIEKRTPTWRDE

Sequences:

>Translated_277_residues
MEIGMSAPALADATATPILLREMAGSVAVLTLNRPAQRNSLSESLIAELHQMLDEIGADKAVRAVVIAANGPAFCAGHDL
KELTARRADADRGRAFFARLMNACSAMMQAIVHMPKPVVASVQGIATAAGCQLVASCDLAVASEHASFATPGVDIGLFCS
TPMVALSRNITRKQAMEMLLTGEPVPAPRAREIGLVNRVVTPGTERETAIALAQQVARKSAHTIKLGKEAFYRQAEMSLA
DAYRYAAEVMTENMLARDAEEGIGAFIEKRTPTWRDE
>Mature_277_residues
MEIGMSAPALADATATPILLREMAGSVAVLTLNRPAQRNSLSESLIAELHQMLDEIGADKAVRAVVIAANGPAFCAGHDL
KELTARRADADRGRAFFARLMNACSAMMQAIVHMPKPVVASVQGIATAAGCQLVASCDLAVASEHASFATPGVDIGLFCS
TPMVALSRNITRKQAMEMLLTGEPVPAPRAREIGLVNRVVTPGTERETAIALAQQVARKSAHTIKLGKEAFYRQAEMSLA
DAYRYAAEVMTENMLARDAEEGIGAFIEKRTPTWRDE

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=261, Percent_Identity=43.6781609195402, Blast_Score=227, Evalue=7e-60,
Organism=Homo sapiens, GI194097323, Length=261, Percent_Identity=31.8007662835249, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4502327, Length=264, Percent_Identity=27.6515151515151, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI221136756, Length=238, Percent_Identity=27.3109243697479, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI221136753, Length=238, Percent_Identity=27.3109243697479, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI221307494, Length=238, Percent_Identity=27.3109243697479, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI70995211, Length=250, Percent_Identity=26.8, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI4757968, Length=260, Percent_Identity=24.6153846153846, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI49355787, Length=260, Percent_Identity=24.6153846153846, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI150378541, Length=276, Percent_Identity=28.6231884057971, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI25453481, Length=199, Percent_Identity=27.6381909547739, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI51538573, Length=199, Percent_Identity=27.6381909547739, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI4757966, Length=199, Percent_Identity=27.6381909547739, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI51538581, Length=199, Percent_Identity=27.6381909547739, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI116235487, Length=207, Percent_Identity=28.0193236714976, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI20127408, Length=185, Percent_Identity=28.1081081081081, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI260274832, Length=252, Percent_Identity=21.4285714285714, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI45643119, Length=252, Percent_Identity=21.4285714285714, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI260275230, Length=252, Percent_Identity=21.4285714285714, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=33.596837944664, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI221142681, Length=258, Percent_Identity=32.1705426356589, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787660, Length=266, Percent_Identity=30.0751879699248, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI87082183, Length=255, Percent_Identity=24.7058823529412, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788597, Length=251, Percent_Identity=25.0996015936255, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788682, Length=199, Percent_Identity=27.6381909547739, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790281, Length=201, Percent_Identity=22.8855721393035, Blast_Score=61, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17540306, Length=258, Percent_Identity=45.3488372093023, Blast_Score=204, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17554946, Length=253, Percent_Identity=30.8300395256917, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25145438, Length=255, Percent_Identity=30.9803921568627, Blast_Score=121, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17536985, Length=282, Percent_Identity=24.468085106383, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=254, Percent_Identity=27.5590551181102, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17540714, Length=267, Percent_Identity=25.8426966292135, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=255, Percent_Identity=25.8823529411765, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17558304, Length=222, Percent_Identity=27.027027027027, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17549921, Length=218, Percent_Identity=27.0642201834862, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=243, Percent_Identity=25.5144032921811, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17508951, Length=228, Percent_Identity=26.7543859649123, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25144276, Length=228, Percent_Identity=26.7543859649123, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508953, Length=228, Percent_Identity=26.7543859649123, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=204, Percent_Identity=26.4705882352941, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=39.0804597701149, Blast_Score=177, Evalue=5e-45,
Organism=Drosophila melanogaster, GI20129971, Length=263, Percent_Identity=29.6577946768061, Blast_Score=121, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24653477, Length=263, Percent_Identity=29.6577946768061, Blast_Score=121, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24653139, Length=272, Percent_Identity=26.8382352941176, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24650670, Length=266, Percent_Identity=28.9473684210526, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21357171, Length=275, Percent_Identity=28, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24654903, Length=195, Percent_Identity=31.7948717948718, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI19920382, Length=251, Percent_Identity=26.2948207171315, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24583165, Length=244, Percent_Identity=23.7704918032787, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 29576; Mature: 29576

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIGMSAPALADATATPILLREMAGSVAVLTLNRPAQRNSLSESLIAELHQMLDEIGADK
CCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCC
AVRAVVIAANGPAFCAGHDLKELTARRADADRGRAFFARLMNACSAMMQAIVHMPKPVVA
CEEEEEEECCCCCEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SVQGIATAAGCQLVASCDLAVASEHASFATPGVDIGLFCSTPMVALSRNITRKQAMEMLL
HHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCEEEECCCHHHHHHHCHHHHHHHHHHH
TGEPVPAPRAREIGLVNRVVTPGTERETAIALAQQVARKSAHTIKLGKEAFYRQAEMSLA
CCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAYRYAAEVMTENMLARDAEEGIGAFIEKRTPTWRDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MEIGMSAPALADATATPILLREMAGSVAVLTLNRPAQRNSLSESLIAELHQMLDEIGADK
CCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCC
AVRAVVIAANGPAFCAGHDLKELTARRADADRGRAFFARLMNACSAMMQAIVHMPKPVVA
CEEEEEEECCCCCEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
SVQGIATAAGCQLVASCDLAVASEHASFATPGVDIGLFCSTPMVALSRNITRKQAMEMLL
HHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCEEEECCCHHHHHHHCHHHHHHHHHHH
TGEPVPAPRAREIGLVNRVVTPGTERETAIALAQQVARKSAHTIKLGKEAFYRQAEMSLA
CCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAYRYAAEVMTENMLARDAEEGIGAFIEKRTPTWRDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]