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The map label for this gene is tig [H]

Identifier: 146341129

GI number: 146341129

Start: 4400026

End: 4401381

Strand: Reverse

Name: tig [H]

Synonym: BRADO4200

Alternate gene names: 146341129

Gene position: 4401381-4400026 (Counterclockwise)

Preceding gene: 146341132

Following gene: 146341127

Centisome position: 59.03

GC content: 64.38

Gene sequence:

>1356_bases
ATGCAGGTCACAGAAACCTTGTCAGAAGGCTTGAAGCATGAGTTCCAGATCAGCGTTCCCGCGGCGGATCTCGACGCCAA
GGCGGATGCCAAGCTGGTCGATCTCAAGGACAAGGTCCGCATCAACGGCTTCCGCCCCGGCAAGGTGCCGGTGGCGCATC
TGAAGAAGATCTACGGCAAGTCGGTCATGGCCGAGACCATCGACCAGACCATCCGCGACACCAACACGCAGATCTTCACC
GAGCGTGGCTTCCGCCTCGCGACCGAGCCGAAGGTGACGATGCCGACCGAGGAGGCCGAGGTTGAGAAGATCCTCGCCGG
CCAGTCCGACCTGACCTACAGCGTCGCGATCGAAGTGGTGCCGGCGATCACGCTCGCCGACTTCAAGACCTTCGCGGTCG
AAAAGCCGGTCGCCGACATCACCGACGCCGACGTCGATGAGGCGATCAAGCGCCTGGCCGATGCCAACCGCAGCTATGCC
GCCAAGGCCGAGGGTGCGAAGGCTGCGAGCGGCGACCGCGTCAAGGTCAACTTCAAGGGCACGATCGACGGCGTCGCCTT
CGACGGCGGCACCGGCGAGGGTATCGACGTCGTGATCGGCTCGAACACCTTCATCCCGGGCTTCGAGGATCAGCTTGTCG
GCATCGCCGTGGGCGAGACCCGCACGCTGAAGGTCACGTTCCCGACCAACTATCTCAACAACGAGCTGGCCGGGAAGGCC
GCGGAGTTCGAGACCACCGCGACCGCCATCGAGGCGCCCGAGGACAAGGTCGTCGACGACGAGTTCGCCAAGACGCTCGG
CCTGGAATCGCTCGACAAGCTGAAGGAGCTGATGCGCGACCGTCTCGCCGGCGAGTTCACCCAGGCGACCCGCCAGCGCG
TCAAGCGCGCGCTGCTCGACCGTCTCGACGAGACCCACAAGTTCGATGCGCCGCCGTCGCTGATCGACGAGGAGTTCAAC
CTGATGTGGAACTCGGTCCAGGCCGAGATGAAGTCGAGCGGCAAGACCTTCGCCGACGAGAACACCACCGAGGACAAGGC
CAAGGAAGAGTACCGCACCATCGCCGACCGCCGCGTGCGTCTCGGTCTGGTGCTGTCGGAGATCGGCGAGAAGAACAAGA
TCACCGTCACCGACGACGAGGTCAGCCGCGCCGTGATCGAGCGCGCGCGCTCGATGCCGGGCCGCGAGAAGGAGGTCTGG
GACTTCTATCGCAGCAACCCGCAGGCGCTCGCACAGCTGCGCGCGCCGATCTACGAGGACAAGGTCGTCGACTTCATCCT
CGAGCTCGCCAACGTCACCGAGAAGAAGGTCTCGAAGGACGAGCTGTTCAAGGACGACGACGACAAGGCGGCCTGA

Upstream 100 bases:

>100_bases
TCCCACCGGCGCAAGGCTCGTTGGCCGCGCGTCGTCCACAATTGAATCAGGTGTGTCCGGATCGCAAGGGTCCGGACCGT
AGCGGAAGAAGATTGGCGCC

Downstream 100 bases:

>100_bases
TCGGGCCACCTCTCGTCGGGTTCGCGACATAAGCGGGAGTTAAGGTTGACCCGTGAAAGCGCGGGTCAGCCAAGTTCCGA
CGCTGGCCGCCGCCGCGGGA

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MQVTETLSEGLKHEFQISVPAADLDAKADAKLVDLKDKVRINGFRPGKVPVAHLKKIYGKSVMAETIDQTIRDTNTQIFT
ERGFRLATEPKVTMPTEEAEVEKILAGQSDLTYSVAIEVVPAITLADFKTFAVEKPVADITDADVDEAIKRLADANRSYA
AKAEGAKAASGDRVKVNFKGTIDGVAFDGGTGEGIDVVIGSNTFIPGFEDQLVGIAVGETRTLKVTFPTNYLNNELAGKA
AEFETTATAIEAPEDKVVDDEFAKTLGLESLDKLKELMRDRLAGEFTQATRQRVKRALLDRLDETHKFDAPPSLIDEEFN
LMWNSVQAEMKSSGKTFADENTTEDKAKEEYRTIADRRVRLGLVLSEIGEKNKITVTDDEVSRAVIERARSMPGREKEVW
DFYRSNPQALAQLRAPIYEDKVVDFILELANVTEKKVSKDELFKDDDDKAA

Sequences:

>Translated_451_residues
MQVTETLSEGLKHEFQISVPAADLDAKADAKLVDLKDKVRINGFRPGKVPVAHLKKIYGKSVMAETIDQTIRDTNTQIFT
ERGFRLATEPKVTMPTEEAEVEKILAGQSDLTYSVAIEVVPAITLADFKTFAVEKPVADITDADVDEAIKRLADANRSYA
AKAEGAKAASGDRVKVNFKGTIDGVAFDGGTGEGIDVVIGSNTFIPGFEDQLVGIAVGETRTLKVTFPTNYLNNELAGKA
AEFETTATAIEAPEDKVVDDEFAKTLGLESLDKLKELMRDRLAGEFTQATRQRVKRALLDRLDETHKFDAPPSLIDEEFN
LMWNSVQAEMKSSGKTFADENTTEDKAKEEYRTIADRRVRLGLVLSEIGEKNKITVTDDEVSRAVIERARSMPGREKEVW
DFYRSNPQALAQLRAPIYEDKVVDFILELANVTEKKVSKDELFKDDDDKAA
>Mature_451_residues
MQVTETLSEGLKHEFQISVPAADLDAKADAKLVDLKDKVRINGFRPGKVPVAHLKKIYGKSVMAETIDQTIRDTNTQIFT
ERGFRLATEPKVTMPTEEAEVEKILAGQSDLTYSVAIEVVPAITLADFKTFAVEKPVADITDADVDEAIKRLADANRSYA
AKAEGAKAASGDRVKVNFKGTIDGVAFDGGTGEGIDVVIGSNTFIPGFEDQLVGIAVGETRTLKVTFPTNYLNNELAGKA
AEFETTATAIEAPEDKVVDDEFAKTLGLESLDKLKELMRDRLAGEFTQATRQRVKRALLDRLDETHKFDAPPSLIDEEFN
LMWNSVQAEMKSSGKTFADENTTEDKAKEEYRTIADRRVRLGLVLSEIGEKNKITVTDDEVSRAVIERARSMPGREKEVW
DFYRSNPQALAQLRAPIYEDKVVDFILELANVTEKKVSKDELFKDDDDKAA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=445, Percent_Identity=32.3595505617978, Blast_Score=199, Evalue=3e-52,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 49891; Mature: 49891

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVTETLSEGLKHEFQISVPAADLDAKADAKLVDLKDKVRINGFRPGKVPVAHLKKIYGK
CCHHHHHHHCCCCEEEEECCCCCCCCCCCCEEECCCCCEEECCCCCCCCCHHHHHHHHHH
SVMAETIDQTIRDTNTQIFTERGFRLATEPKVTMPTEEAEVEKILAGQSDLTYSVAIEVV
HHHHHHHHHHHHHCCCEEEECCCCEEECCCCCCCCCCHHHHHHHHCCCCCCEEEEEEHHH
PAITLADFKTFAVEKPVADITDADVDEAIKRLADANRSYAAKAEGAKAASGDRVKVNFKG
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEEEEEEE
TIDGVAFDGGTGEGIDVVIGSNTFIPGFEDQLVGIAVGETRTLKVTFPTNYLNNELAGKA
EECEEEECCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCEEEEEEECCHHHCHHHCCCC
AEFETTATAIEAPEDKVVDDEFAKTLGLESLDKLKELMRDRLAGEFTQATRQRVKRALLD
HHHHHHHHHHCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLDETHKFDAPPSLIDEEFNLMWNSVQAEMKSSGKTFADENTTEDKAKEEYRTIADRRVR
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LGLVLSEIGEKNKITVTDDEVSRAVIERARSMPGREKEVWDFYRSNPQALAQLRAPIYED
HHHHHHHCCCCCCEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHCCHHHH
KVVDFILELANVTEKKVSKDELFKDDDDKAA
HHHHHHHHHHHHHHHHCCHHHCCCCCCCCCC
>Mature Secondary Structure
MQVTETLSEGLKHEFQISVPAADLDAKADAKLVDLKDKVRINGFRPGKVPVAHLKKIYGK
CCHHHHHHHCCCCEEEEECCCCCCCCCCCCEEECCCCCEEECCCCCCCCCHHHHHHHHHH
SVMAETIDQTIRDTNTQIFTERGFRLATEPKVTMPTEEAEVEKILAGQSDLTYSVAIEVV
HHHHHHHHHHHHHCCCEEEECCCCEEECCCCCCCCCCHHHHHHHHCCCCCCEEEEEEHHH
PAITLADFKTFAVEKPVADITDADVDEAIKRLADANRSYAAKAEGAKAASGDRVKVNFKG
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEEEEEEE
TIDGVAFDGGTGEGIDVVIGSNTFIPGFEDQLVGIAVGETRTLKVTFPTNYLNNELAGKA
EECEEEECCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCEEEEEEECCHHHCHHHCCCC
AEFETTATAIEAPEDKVVDDEFAKTLGLESLDKLKELMRDRLAGEFTQATRQRVKRALLD
HHHHHHHHHHCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLDETHKFDAPPSLIDEEFNLMWNSVQAEMKSSGKTFADENTTEDKAKEEYRTIADRRVR
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LGLVLSEIGEKNKITVTDDEVSRAVIERARSMPGREKEVWDFYRSNPQALAQLRAPIYED
HHHHHHHCCCCCCEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHCCHHHH
KVVDFILELANVTEKKVSKDELFKDDDDKAA
HHHHHHHHHHHHHHHHCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA