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The map label for this gene is nuoM [H]

Identifier: 146341101

GI number: 146341101

Start: 4373580

End: 4375088

Strand: Reverse

Name: nuoM [H]

Synonym: BRADO4172

Alternate gene names: 146341101

Gene position: 4375088-4373580 (Counterclockwise)

Preceding gene: 146341102

Following gene: 146341100

Centisome position: 58.67

GC content: 63.82

Gene sequence:

>1509_bases
ATGACCACCTGGCCAATTCTCTCTGTCGTCACCTTCCTGCCGCTCGTCGGCGCGCTGCTGATCTATCTGGTCCGTGGCGA
CGACGAGGCCGCCCAGCGCAATGCGCGCTGGATCGCGCTGTGGACCACGCTGATCACCTTCGCGGTGTCGGTGATCCTGG
TGCTGCGCTTCGACCCCAACCTGACCGACTTCCAGTTCGTCGAGAAGAAGAACTGGCTGGCGGCCGGCATCAGCTACCAC
ATGGGTGTCGACGGCATCTCGCTGCCATTCGTGATCCTGACCACCGCCTTGATGCCGTTTTGCATCATCGCTAGCTGGAA
GTCGGTGAAGAACCGCCTGCGCGAATACATGATGGCGTTCCTGATCCTGGAAACGCTGATGGTCGGCACCTTCTCGGCGC
TCGACCTCGTGCTGTTCTACCTGTTCTTCGAGGGCGGCCTGATCCCGATGTTCCTGATCATCGGCGTCTGGGGCGGCCCG
CGCCGGGTCTACGCGTCGTTCAAGTTCTTCCTCTACACCTTGCTCGGCTCGGTGCTGATGCTGCTCGCCATCATGGCGCT
GTACTGGAACGCCGGCACGACCGACATCCCGACCCTGATGCACACGGCGGTGCCGCGCAGCTTGCAGACCTGGGCCTGGC
TTGCCTTCTTCGCCTCCTTCGCGGTGAAGATGCCGATGTGGCCGGTGCACACCTGGCTGCCGGATGCGCACGTCGAGGCG
CCGACCGCCGGCTCGGTGATCCTGGCCGCGATCCTGCTGAAGATGGGCGGCTACGGCTTCCTGCGCTTCTCGCTGCCGAT
GTTCCCGCTGGCCTCGCACGACTTCGCGCCGCTGGTGTGGACGCTGTCGGCGATCGCGATCATCTACACCTCGCTGGTCG
CGCTGATGCAGGAGGACATCAAGAAGCTCATCGCCTACTCGTCGGTGGCGCATATGGGCTTCGTGACGATGGGCATCTTC
GCCGGCACCATGCAGGGTGTCGCCGGCGGCGTGTTCCAGATGATCTCGCACGGCATCGTCTCGGGCGCGCTGTTCCTTTG
CGTCGGCGTCGTCTACGACCGCATGCACACCCGCGAGATCGCGGCCTATGGCGGCCTCGTCAACCGGATGCCGGTCTACG
CGCTGGTGTTCATGGTGTTCACCATGGCCAATGTCGGTCTGCCCGGCACCTCCGGCTTCGTCGGCGAGTTCATGACCCTG
CTCGGCACCTTCAAGGTGTCGATTCCGACCGCGTTCTTCGCAACCTTCGGCGTCATCCTGTCGGCCGCCTATGCGCTGTG
GCTGTATCGCAAGGTGGTGTTCGGCGCGCTGACCAAGCCGTCGCTCGCGAGCATCAAGGATCTCACCTGGCGTGAAGGCA
TGATCCTGTTTCCGCTGGTGGCGCTGACCATCCTGTTCGGCGTCTATCCGAAGCCGATCCTGGACATGTCGGCGGCCTCG
GTGCAGCAGCTCGTCACCAACTACAATACCGCCGTGACGGCCGTGAAGGCCGCCGCGCTGCTCCAATGA

Upstream 100 bases:

>100_bases
TCGTCAAGATCCAGACCGGTTATCTCTATCACTATGCCTTTGCCATGCTGATCGGCGTCGCCGGCCTGATCACCTGGTTC
ATGTTCATGGGGGGCCAGCA

Downstream 100 bases:

>100_bases
TGAGACCTTAAGGGGTCGGGACAGAGACGATGATCGAGAATCTTGGGCCTCAGCTTTGGGTGGTGCTGCCGGAGCTGCTG
CTGGCCTTGGGGGCCATGGC

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I, chain 13; NDH-1, chain 13 [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MTTWPILSVVTFLPLVGALLIYLVRGDDEAAQRNARWIALWTTLITFAVSVILVLRFDPNLTDFQFVEKKNWLAAGISYH
MGVDGISLPFVILTTALMPFCIIASWKSVKNRLREYMMAFLILETLMVGTFSALDLVLFYLFFEGGLIPMFLIIGVWGGP
RRVYASFKFFLYTLLGSVLMLLAIMALYWNAGTTDIPTLMHTAVPRSLQTWAWLAFFASFAVKMPMWPVHTWLPDAHVEA
PTAGSVILAAILLKMGGYGFLRFSLPMFPLASHDFAPLVWTLSAIAIIYTSLVALMQEDIKKLIAYSSVAHMGFVTMGIF
AGTMQGVAGGVFQMISHGIVSGALFLCVGVVYDRMHTREIAAYGGLVNRMPVYALVFMVFTMANVGLPGTSGFVGEFMTL
LGTFKVSIPTAFFATFGVILSAAYALWLYRKVVFGALTKPSLASIKDLTWREGMILFPLVALTILFGVYPKPILDMSAAS
VQQLVTNYNTAVTAVKAAALLQ

Sequences:

>Translated_502_residues
MTTWPILSVVTFLPLVGALLIYLVRGDDEAAQRNARWIALWTTLITFAVSVILVLRFDPNLTDFQFVEKKNWLAAGISYH
MGVDGISLPFVILTTALMPFCIIASWKSVKNRLREYMMAFLILETLMVGTFSALDLVLFYLFFEGGLIPMFLIIGVWGGP
RRVYASFKFFLYTLLGSVLMLLAIMALYWNAGTTDIPTLMHTAVPRSLQTWAWLAFFASFAVKMPMWPVHTWLPDAHVEA
PTAGSVILAAILLKMGGYGFLRFSLPMFPLASHDFAPLVWTLSAIAIIYTSLVALMQEDIKKLIAYSSVAHMGFVTMGIF
AGTMQGVAGGVFQMISHGIVSGALFLCVGVVYDRMHTREIAAYGGLVNRMPVYALVFMVFTMANVGLPGTSGFVGEFMTL
LGTFKVSIPTAFFATFGVILSAAYALWLYRKVVFGALTKPSLASIKDLTWREGMILFPLVALTILFGVYPKPILDMSAAS
VQQLVTNYNTAVTAVKAAALLQ
>Mature_501_residues
TTWPILSVVTFLPLVGALLIYLVRGDDEAAQRNARWIALWTTLITFAVSVILVLRFDPNLTDFQFVEKKNWLAAGISYHM
GVDGISLPFVILTTALMPFCIIASWKSVKNRLREYMMAFLILETLMVGTFSALDLVLFYLFFEGGLIPMFLIIGVWGGPR
RVYASFKFFLYTLLGSVLMLLAIMALYWNAGTTDIPTLMHTAVPRSLQTWAWLAFFASFAVKMPMWPVHTWLPDAHVEAP
TAGSVILAAILLKMGGYGFLRFSLPMFPLASHDFAPLVWTLSAIAIIYTSLVALMQEDIKKLIAYSSVAHMGFVTMGIFA
GTMQGVAGGVFQMISHGIVSGALFLCVGVVYDRMHTREIAAYGGLVNRMPVYALVFMVFTMANVGLPGTSGFVGEFMTLL
GTFKVSIPTAFFATFGVILSAAYALWLYRKVVFGALTKPSLASIKDLTWREGMILFPLVALTILFGVYPKPILDMSAASV
QQLVTNYNTAVTAVKAAALLQ

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=373, Percent_Identity=36.9973190348526, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI251831117, Length=361, Percent_Identity=24.3767313019391, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1788613, Length=504, Percent_Identity=38.4920634920635, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1788827, Length=261, Percent_Identity=27.5862068965517, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1788831, Length=277, Percent_Identity=25.9927797833935, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1788614, Length=438, Percent_Identity=24.8858447488584, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI2367154, Length=353, Percent_Identity=26.3456090651558, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI145693160, Length=353, Percent_Identity=25.7790368271955, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788829, Length=298, Percent_Identity=26.1744966442953, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 55273; Mature: 55142

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTWPILSVVTFLPLVGALLIYLVRGDDEAAQRNARWIALWTTLITFAVSVILVLRFDPN
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCC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HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]