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The map label for this gene is nuoN [H]

Identifier: 146341100

GI number: 146341100

Start: 4372123

End: 4373550

Strand: Reverse

Name: nuoN [H]

Synonym: BRADO4171

Alternate gene names: 146341100

Gene position: 4373550-4372123 (Counterclockwise)

Preceding gene: 146341101

Following gene: 146341099

Centisome position: 58.65

GC content: 64.92

Gene sequence:

>1428_bases
ATGATCGAGAATCTTGGGCCTCAGCTTTGGGTGGTGCTGCCGGAGCTGCTGCTGGCCTTGGGGGCCATGGCGCTGTTGAT
GCTGGGCGCGTTTCGCGGCCAGGGGACGACGGGGATCGTCACCGGCCTCGCGGTCGTGCTGCTGATCGTGGTCGGCATCC
TGGTGCTGAGGCAGCCGGCCGGGCAGGCGTTCGGCGGCAGCTTCATCGCCGACAGCTATGCGCGCTTTCTGAAGATCCTG
GCGATCATCGGCTCGATCGGCACGCTGGTGATGTCGGCGGAGTTTCTCTCCAAGCCGGACCGCCGCATCTTCGAATATGC
GATCCTGGTGCTGCTCTCGACCTTGGGCATGCTGGTCCTGATCTCGGCCGGCGATCTGATCGCGCTCTATCTCGGCCTCG
AGCTGATGTCGCTCGCGCTTTACGTCGTCGCCGCCAGCCATCGCGACAACGTCAAGTCGACCGAGGCCGGCCTGAAGTAC
TTCGTGCTCGGCGCGCTTTCGTCCGGCATGCTCCTCTACGGCGCCTCGCTGGTCTACGGCTTCACCGGCACGGTGAGCTT
TGCCGGAATTGCCGCGGCGACCAAGACCGGCAGCGTCGGCGTGATCTTCGGCCTCGTCTTCCTGTTCGCCGGCATGTGCT
TCAAGGTCTCGGCCGTGCCGTTCCACATGTGGACGCCGGACGTCTATGAAGGCGCGCCGACGCCGGTGACGGCGTTCTTC
GCCTCGGCACCGAAGGTCGCCGCGATCGCCGTGTTCGCGCGCGTCGCGCTCACCGCGTTCCCGGGCATCACCTTCCAGTG
GCAGCAGATCATCGTGTTCGTTTCGATCGCCTCGATGGCGCTCGGCTCGTTCGCCGCGATCGGCCAGACCAACATCAAGC
GGCTGATGGCCTACTCGTCGATCGGCCACATGGGCTTCGCGCTGGTCGGCCTCGCCGCCGGCACGGTGGAAGGCGCGCAG
GGCGTGCTGACCTATATCGCGATCTATGTCGCGATGACGCTGGGCTCATTCGCGGTGATCATGGCGATCAAGCGCAACGG
CCAGTCGTTCGAGAAGATCAGCGACTTCGCCGGTCTGTCCCGCACCAACCCGCTGCTGGCCTTCTTCTTCGCAATGCTCT
TGTTCTCGCTGGCCGGCGTCCCGCCACTCGCGGGCTTCTTCGCCAAATGGTACGTCTTCGTCGCCGCGATCAAGGGCGGT
CTGTTCACGCTGTCGGTGATCGGCGTGCTGACCAGCGTCGTCGGCGCCTACTATTATCTGCTCATCGTCAAGACGATGTA
TTTCGACCAGCCGCTGCCGACGGTCGATCCGGTCCGGATCGAGCTGCGCACCGTGCTCGCGATCTCCGGCATCTTCAACA
TCCTGTTCTTCGCCTATCCCGGCCCGCTGGTCAGCGCCGCTGCGGCCGCGGCGAAGTCGCTGTTCTAG

Upstream 100 bases:

>100_bases
CGGTGCAGCAGCTCGTCACCAACTACAATACCGCCGTGACGGCCGTGAAGGCCGCCGCGCTGCTCCAATGATGAGACCTT
AAGGGGTCGGGACAGAGACG

Downstream 100 bases:

>100_bases
ATGACGTTGGCGCTCGGAGCCCGTGCCGCTGCCGGCGGCTACGGGCTCGTCGTCTTCGATCAGGCCGGCTCGACCAATGT
CGAGGCCTTGGTCGCGGCGC

Product: NADH dehydrogenase subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MIENLGPQLWVVLPELLLALGAMALLMLGAFRGQGTTGIVTGLAVVLLIVVGILVLRQPAGQAFGGSFIADSYARFLKIL
AIIGSIGTLVMSAEFLSKPDRRIFEYAILVLLSTLGMLVLISAGDLIALYLGLELMSLALYVVAASHRDNVKSTEAGLKY
FVLGALSSGMLLYGASLVYGFTGTVSFAGIAAATKTGSVGVIFGLVFLFAGMCFKVSAVPFHMWTPDVYEGAPTPVTAFF
ASAPKVAAIAVFARVALTAFPGITFQWQQIIVFVSIASMALGSFAAIGQTNIKRLMAYSSIGHMGFALVGLAAGTVEGAQ
GVLTYIAIYVAMTLGSFAVIMAIKRNGQSFEKISDFAGLSRTNPLLAFFFAMLLFSLAGVPPLAGFFAKWYVFVAAIKGG
LFTLSVIGVLTSVVGAYYYLLIVKTMYFDQPLPTVDPVRIELRTVLAISGIFNILFFAYPGPLVSAAAAAAKSLF

Sequences:

>Translated_475_residues
MIENLGPQLWVVLPELLLALGAMALLMLGAFRGQGTTGIVTGLAVVLLIVVGILVLRQPAGQAFGGSFIADSYARFLKIL
AIIGSIGTLVMSAEFLSKPDRRIFEYAILVLLSTLGMLVLISAGDLIALYLGLELMSLALYVVAASHRDNVKSTEAGLKY
FVLGALSSGMLLYGASLVYGFTGTVSFAGIAAATKTGSVGVIFGLVFLFAGMCFKVSAVPFHMWTPDVYEGAPTPVTAFF
ASAPKVAAIAVFARVALTAFPGITFQWQQIIVFVSIASMALGSFAAIGQTNIKRLMAYSSIGHMGFALVGLAAGTVEGAQ
GVLTYIAIYVAMTLGSFAVIMAIKRNGQSFEKISDFAGLSRTNPLLAFFFAMLLFSLAGVPPLAGFFAKWYVFVAAIKGG
LFTLSVIGVLTSVVGAYYYLLIVKTMYFDQPLPTVDPVRIELRTVLAISGIFNILFFAYPGPLVSAAAAAAKSLF
>Mature_475_residues
MIENLGPQLWVVLPELLLALGAMALLMLGAFRGQGTTGIVTGLAVVLLIVVGILVLRQPAGQAFGGSFIADSYARFLKIL
AIIGSIGTLVMSAEFLSKPDRRIFEYAILVLLSTLGMLVLISAGDLIALYLGLELMSLALYVVAASHRDNVKSTEAGLKY
FVLGALSSGMLLYGASLVYGFTGTVSFAGIAAATKTGSVGVIFGLVFLFAGMCFKVSAVPFHMWTPDVYEGAPTPVTAFF
ASAPKVAAIAVFARVALTAFPGITFQWQQIIVFVSIASMALGSFAAIGQTNIKRLMAYSSIGHMGFALVGLAAGTVEGAQ
GVLTYIAIYVAMTLGSFAVIMAIKRNGQSFEKISDFAGLSRTNPLLAFFFAMLLFSLAGVPPLAGFFAKWYVFVAAIKGG
LFTLSVIGVLTSVVGAYYYLLIVKTMYFDQPLPTVDPVRIELRTVLAISGIFNILFFAYPGPLVSAAAAAAKSLF

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Homo sapiens, GI251831108, Length=294, Percent_Identity=23.469387755102, Blast_Score=76, Evalue=8e-14,
Organism=Escherichia coli, GI145693160, Length=408, Percent_Identity=33.8235294117647, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1788614, Length=356, Percent_Identity=26.9662921348315, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788831, Length=388, Percent_Identity=24.7422680412371, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1788613, Length=281, Percent_Identity=26.6903914590747, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788827, Length=315, Percent_Identity=26.031746031746, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788829, Length=256, Percent_Identity=25.78125, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 50086; Mature: 50086

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA