Click here to switch to the map view.

The map label for this gene is tsf

Identifier: 146341068

GI number: 146341068

Start: 4334988

End: 4335911

Strand: Reverse

Name: tsf

Synonym: BRADO4139

Alternate gene names: 146341068

Gene position: 4335911-4334988 (Counterclockwise)

Preceding gene: 146341069

Following gene: 146341067

Centisome position: 58.15

GC content: 66.23

Gene sequence:

>924_bases
ATGGCGAATATCACCGCAGCGATGGTGAAAGACCTGCGCGAGTCGACCGGCGCGGGCATGATGGATTGCAAGGCTGCGCT
CACCGAGACCGGCGGCGACATGCAGGCGGCCCAGGATTGGCTGCGCAAAAAGGGCCTGTCGAAGGCCGCCAAGAAGGCCG
GCCGCGTCGCGGCCGAGGGCCTGATCGGCGCGCTGACCTCGGGCAAGAAGGGCGTGGTCGTCGAGGTCAACTCCGAGACC
GACTTCGTCGCGCGCAACGAGCACTTCCAGGGCCTGGTCAAGATGATCGCCCAGGTCGCGCTCGACGTCGGCGCCGATGT
CGAGAAGATCAAGGCCGCCAAGGTCGGCAGCATCACGGTCGAGGCCGCGATCGCCGATTCGATCGCGACCATCGGTGAGA
ACCAGTCGCTGCGCCGCGCGGCCGCGCTCGAAGTGAGCGAGGGCGTGGTGGCGAGCTACGTCCACGGCGCGGTCATCGAG
GGCGCCGGCAAGCTCGGCGTCATCGTTGCGCTGGAGTCGCCGGGCAAGACCGACGAACTCGCGGCGCTCGGCCGTCAGCT
CGCGATGCATGTCGCCGCCGCCAACCCGCAGGCGATCGATGCCGCCGGTCTCGATCCGGAGGTCGTCAAGCGCGAGAAGG
ACGTGCTCTCCGACAAATATCGGCAGCAGGGCAAGCCGGAAAACGTGATCGAGAAGATCGTCGAGTCCGGCCTGAAGACC
TATTACAAGGAAGTCACCCTGCTCGAGCAGGCCTTTATCCACGACAGCGGCAAGTCCGTTGCCCAGGCGCTGAAGGAGGC
CGAAGGCAAGGTCGGCGGTCCGATCAAGGTGGCCGGTTTCGTCCGCTATGCGCTCGGTGAGGGCATCGAGAAGGAAGAGA
CCGACTTCGCCGCCGAAGTCGCCGCCGCCAGCGGCAAGAAGTAA

Upstream 100 bases:

>100_bases
CGCACGCCGGCCATCGCGACTGAGATAGGGTTTCCTGGCAGCGGACGGCGATGAGCCATCCGCTGCGTCGCGCTTTGGAC
AGGCAAAAGGACGTTCAACA

Downstream 100 bases:

>100_bases
CGACTGACATCAGTCACGATCCGGCGCGGACGTCGCGCCGGATCTCAACGCGCGAGATTGGGACATATTGGCACATGGCT
GAGCCGGTCTATCGTCGCGT

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MANITAAMVKDLRESTGAGMMDCKAALTETGGDMQAAQDWLRKKGLSKAAKKAGRVAAEGLIGALTSGKKGVVVEVNSET
DFVARNEHFQGLVKMIAQVALDVGADVEKIKAAKVGSITVEAAIADSIATIGENQSLRRAAALEVSEGVVASYVHGAVIE
GAGKLGVIVALESPGKTDELAALGRQLAMHVAAANPQAIDAAGLDPEVVKREKDVLSDKYRQQGKPENVIEKIVESGLKT
YYKEVTLLEQAFIHDSGKSVAQALKEAEGKVGGPIKVAGFVRYALGEGIEKEETDFAAEVAAASGKK

Sequences:

>Translated_307_residues
MANITAAMVKDLRESTGAGMMDCKAALTETGGDMQAAQDWLRKKGLSKAAKKAGRVAAEGLIGALTSGKKGVVVEVNSET
DFVARNEHFQGLVKMIAQVALDVGADVEKIKAAKVGSITVEAAIADSIATIGENQSLRRAAALEVSEGVVASYVHGAVIE
GAGKLGVIVALESPGKTDELAALGRQLAMHVAAANPQAIDAAGLDPEVVKREKDVLSDKYRQQGKPENVIEKIVESGLKT
YYKEVTLLEQAFIHDSGKSVAQALKEAEGKVGGPIKVAGFVRYALGEGIEKEETDFAAEVAAASGKK
>Mature_306_residues
ANITAAMVKDLRESTGAGMMDCKAALTETGGDMQAAQDWLRKKGLSKAAKKAGRVAAEGLIGALTSGKKGVVVEVNSETD
FVARNEHFQGLVKMIAQVALDVGADVEKIKAAKVGSITVEAAIADSIATIGENQSLRRAAALEVSEGVVASYVHGAVIEG
AGKLGVIVALESPGKTDELAALGRQLAMHVAAANPQAIDAAGLDPEVVKREKDVLSDKYRQQGKPENVIEKIVESGLKTY
YKEVTLLEQAFIHDSGKSVAQALKEAEGKVGGPIKVAGFVRYALGEGIEKEETDFAAEVAAASGKK

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Homo sapiens, GI171846268, Length=232, Percent_Identity=36.6379310344828, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI291084500, Length=251, Percent_Identity=34.2629482071713, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI291084502, Length=108, Percent_Identity=47.2222222222222, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI291084498, Length=108, Percent_Identity=47.2222222222222, Blast_Score=92, Evalue=8e-19,
Organism=Escherichia coli, GI1786366, Length=306, Percent_Identity=46.078431372549, Blast_Score=219, Evalue=3e-58,
Organism=Caenorhabditis elegans, GI17561440, Length=304, Percent_Identity=29.6052631578947, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19921466, Length=307, Percent_Identity=27.6872964169381, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_BRASO (A4YVG5)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001206116.1
- ProteinModelPortal:   A4YVG5
- SMR:   A4YVG5
- STRING:   A4YVG5
- GeneID:   5116958
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO4139
- eggNOG:   COG0264
- HOGENOM:   HBG713289
- OMA:   YLHGTRI
- ProtClustDB:   PRK09377
- BioCyc:   BSP376:BRADO4139-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; PF00627 UBA; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 31969; Mature: 31838

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANITAAMVKDLRESTGAGMMDCKAALTETGGDMQAAQDWLRKKGLSKAAKKAGRVAAEG
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHH
LIGALTSGKKGVVVEVNSETDFVARNEHFQGLVKMIAQVALDVGADVEKIKAAKVGSITV
HHHHHCCCCCCEEEEECCCCCCHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEE
EAAIADSIATIGENQSLRRAAALEVSEGVVASYVHGAVIEGAGKLGVIVALESPGKTDEL
HHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHH
AALGRQLAMHVAAANPQAIDAAGLDPEVVKREKDVLSDKYRQQGKPENVIEKIVESGLKT
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
YYKEVTLLEQAFIHDSGKSVAQALKEAEGKVGGPIKVAGFVRYALGEGIEKEETDFAAEV
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
AAASGKK
HHHCCCC
>Mature Secondary Structure 
ANITAAMVKDLRESTGAGMMDCKAALTETGGDMQAAQDWLRKKGLSKAAKKAGRVAAEG
CCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHH
LIGALTSGKKGVVVEVNSETDFVARNEHFQGLVKMIAQVALDVGADVEKIKAAKVGSITV
HHHHHCCCCCCEEEEECCCCCCHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEE
EAAIADSIATIGENQSLRRAAALEVSEGVVASYVHGAVIEGAGKLGVIVALESPGKTDEL
HHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHH
AALGRQLAMHVAAANPQAIDAAGLDPEVVKREKDVLSDKYRQQGKPENVIEKIVESGLKT
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
YYKEVTLLEQAFIHDSGKSVAQALKEAEGKVGGPIKVAGFVRYALGEGIEKEETDFAAEV
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
AAASGKK
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA