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The map label for this gene is pdhC [H]

Identifier: 146341013

GI number: 146341013

Start: 4274105

End: 4275463

Strand: Reverse

Name: pdhC [H]

Synonym: BRADO4084

Alternate gene names: 146341013

Gene position: 4275463-4274105 (Counterclockwise)

Preceding gene: 146341014

Following gene: 146341012

Centisome position: 57.34

GC content: 67.33

Gene sequence:

>1359_bases
ATGCCGATCAACATTCTGATGCCCGCGCTGTCGCCGACGATGGAGAAGGGCAACCTCGCTCGTTGGCTCAAGAAGGAAGG
CGACCAGGTCAAGTCGGGCGAGGTCATCGCCGAGATCGAGACCGACAAGGCCACGATGGAAGTCGAGGCGGTCGACGAAG
GCACGCTCGCCAAGATCCTGGTGCCCGAGGGCACGCAGGACGTGCCGGTCAACGACGTGATCGCCGTGCTCGCCGGGGAG
GGTGAGGACGTCAAGGCGGCGGGCAGCGCGCCGGCGACTGCTGCGCCGAAGGCTGAAGCCAAGCCGACGGCTTCTGCGGC
TCCCGCTGCTGCGCCAGCGCCTGCTGCCGCGCCCGCGCCGAAGCCGGCTGCGGCTCCCGCACCGTCCGCTCCTGCGGCCG
CTGCACCCCAGGTCAACGGCCACGCCCGCATCTTCTCGTCGCCGCTGGCGCGGCGCCTCGCCAAGGACGCCGGCATCGAT
CTCGGCCGTATCACTGGCACCGGCCCGCATGGCCGCGTCGTCGCCCGCGACGTCGAGGAGGCCAAGTCCGGCAAGGGCCT
GAAGGCCGCGCCGTCGGCCGCTCCCGCTGCCGCAGGCGCGCCGGCCGTGGCGCCGTCGATGTCCGACAAGCAGATCCTCG
CGCTGTTCGAGCCCGGCTCCTACGAAGTCATCCCGCATGACGGCATGCGTCGCACCATCGCGCAGCGCCTGACCGCCGCC
ACGCAGACCGTCCCGCATTTCTATCTCACGATCGACTGCGACATCGGCAAGCTCTTGGCCGCGCGCGAGGAGATCAATGC
GGCAGCTCCAAAGGACAAGGAGAAGAAGCCGCTCTACAAGCTCTCGGTGAACGACTTCGTCATCAAGGCGATGGCGGTGG
CGCTGCAGAAGATCCCCAACTGCAACGTCAGCTGGACTGAAGGCGGCATGGTCAAGCACAAGCATTCCGACGTCGGCGTT
GCGGTTGCGATGCCGGGCGGACTGATCACCCCGATCATCCGCAAGGCCGAGACGAAGACGCTGTCGGCGATCTCGGGCGA
GATGAAGGATTTTGCCGCGCGCGCCCGCTCCCGCAAGCTGAAGCCGGAGGAATATCAGGGCGGCACGACCGCCGTGTCGA
ACCTCGGCATGTACGGCATCACGCACTTCACGGCGGTGATCAATCCGCCGCATGCGACGATTCTGGCGGTCGGCACATCA
GAAGAGCGGCCGGTCGTGCGCAACGGCAAGATCGAGATCGCCAGCATGATGAGCGTGACCCTGTCGTGCGACCACCGCGC
CATCGACGGCGCGCTCGGCGCCGAGCTGATCGGCGCCTTCAAGCAGCTGATCGAGAACCCCGTGATGATGATGGTGTGA

Upstream 100 bases:

>100_bases
ACGAGGCGCTGCAGCGCATCGTCGAGATGTTCGAAGCCGAGCTGGCTCGGCCGACTGATATGGGCACCACCAGCCCCAGG
TCGAAACAAGGTCACTGATC

Downstream 100 bases:

>100_bases
GCCGAAGGGCTCATGCCCGTCATCCCAATCGAACACTTGTAGGGTGGGCAAAGCGCAGCGTGCCCACCGACCACATCGCA
AGCTCGTGAGACGGTGGGCA

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MPINILMPALSPTMEKGNLARWLKKEGDQVKSGEVIAEIETDKATMEVEAVDEGTLAKILVPEGTQDVPVNDVIAVLAGE
GEDVKAAGSAPATAAPKAEAKPTASAAPAAAPAPAAAPAPKPAAAPAPSAPAAAAPQVNGHARIFSSPLARRLAKDAGID
LGRITGTGPHGRVVARDVEEAKSGKGLKAAPSAAPAAAGAPAVAPSMSDKQILALFEPGSYEVIPHDGMRRTIAQRLTAA
TQTVPHFYLTIDCDIGKLLAAREEINAAAPKDKEKKPLYKLSVNDFVIKAMAVALQKIPNCNVSWTEGGMVKHKHSDVGV
AVAMPGGLITPIIRKAETKTLSAISGEMKDFAARARSRKLKPEEYQGGTTAVSNLGMYGITHFTAVINPPHATILAVGTS
EERPVVRNGKIEIASMMSVTLSCDHRAIDGALGAELIGAFKQLIENPVMMMV

Sequences:

>Translated_452_residues
MPINILMPALSPTMEKGNLARWLKKEGDQVKSGEVIAEIETDKATMEVEAVDEGTLAKILVPEGTQDVPVNDVIAVLAGE
GEDVKAAGSAPATAAPKAEAKPTASAAPAAAPAPAAAPAPKPAAAPAPSAPAAAAPQVNGHARIFSSPLARRLAKDAGID
LGRITGTGPHGRVVARDVEEAKSGKGLKAAPSAAPAAAGAPAVAPSMSDKQILALFEPGSYEVIPHDGMRRTIAQRLTAA
TQTVPHFYLTIDCDIGKLLAAREEINAAAPKDKEKKPLYKLSVNDFVIKAMAVALQKIPNCNVSWTEGGMVKHKHSDVGV
AVAMPGGLITPIIRKAETKTLSAISGEMKDFAARARSRKLKPEEYQGGTTAVSNLGMYGITHFTAVINPPHATILAVGTS
EERPVVRNGKIEIASMMSVTLSCDHRAIDGALGAELIGAFKQLIENPVMMMV
>Mature_451_residues
PINILMPALSPTMEKGNLARWLKKEGDQVKSGEVIAEIETDKATMEVEAVDEGTLAKILVPEGTQDVPVNDVIAVLAGEG
EDVKAAGSAPATAAPKAEAKPTASAAPAAAPAPAAAPAPKPAAAPAPSAPAAAAPQVNGHARIFSSPLARRLAKDAGIDL
GRITGTGPHGRVVARDVEEAKSGKGLKAAPSAAPAAAGAPAVAPSMSDKQILALFEPGSYEVIPHDGMRRTIAQRLTAAT
QTVPHFYLTIDCDIGKLLAAREEINAAAPKDKEKKPLYKLSVNDFVIKAMAVALQKIPNCNVSWTEGGMVKHKHSDVGVA
VAMPGGLITPIIRKAETKTLSAISGEMKDFAARARSRKLKPEEYQGGTTAVSNLGMYGITHFTAVINPPHATILAVGTSE
ERPVVRNGKIEIASMMSVTLSCDHRAIDGALGAELIGAFKQLIENPVMMMV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=452, Percent_Identity=42.0353982300885, Blast_Score=342, Evalue=6e-94,
Organism=Homo sapiens, GI203098816, Length=475, Percent_Identity=38.1052631578947, Blast_Score=288, Evalue=8e-78,
Organism=Homo sapiens, GI203098753, Length=475, Percent_Identity=38.1052631578947, Blast_Score=288, Evalue=1e-77,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=43.4782608695652, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI19923748, Length=226, Percent_Identity=33.6283185840708, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI110671329, Length=445, Percent_Identity=25.3932584269663, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1786946, Length=450, Percent_Identity=29.7777777777778, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1786305, Length=299, Percent_Identity=34.4481605351171, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17560088, Length=452, Percent_Identity=41.1504424778761, Blast_Score=325, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI17538894, Length=311, Percent_Identity=35.048231511254, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI25146366, Length=451, Percent_Identity=31.0421286031042, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17537937, Length=440, Percent_Identity=29.3181818181818, Blast_Score=147, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6324258, Length=456, Percent_Identity=43.2017543859649, Blast_Score=325, Evalue=9e-90,
Organism=Saccharomyces cerevisiae, GI6320352, Length=451, Percent_Identity=27.4944567627494, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6321632, Length=78, Percent_Identity=51.2820512820513, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI20129315, Length=463, Percent_Identity=40.6047516198704, Blast_Score=275, Evalue=6e-74,
Organism=Drosophila melanogaster, GI24582497, Length=452, Percent_Identity=40.0442477876106, Blast_Score=261, Evalue=8e-70,
Organism=Drosophila melanogaster, GI18859875, Length=446, Percent_Identity=27.5784753363229, Blast_Score=144, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24645909, Length=249, Percent_Identity=31.7269076305221, Blast_Score=108, Evalue=5e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 46888; Mature: 46756

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPINILMPALSPTMEKGNLARWLKKEGDQVKSGEVIAEIETDKATMEVEAVDEGTLAKIL
CCCEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEECCCCCEEEEE
VPEGTQDVPVNDVIAVLAGEGEDVKAAGSAPATAAPKAEAKPTASAAPAAAPAPAAAPAP
ECCCCCCCCHHHEEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KPAAAPAPSAPAAAAPQVNGHARIFSSPLARRLAKDAGIDLGRITGTGPHGRVVARDVEE
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEHHHHH
AKSGKGLKAAPSAAPAAAGAPAVAPSMSDKQILALFEPGSYEVIPHDGMRRTIAQRLTAA
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCHHHHHHHHHHHHH
TQTVPHFYLTIDCDIGKLLAAREEINAAAPKDKEKKPLYKLSVNDFVIKAMAVALQKIPN
HHCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCC
CNVSWTEGGMVKHKHSDVGVAVAMPGGLITPIIRKAETKTLSAISGEMKDFAARARSRKL
CCEEECCCCEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KPEEYQGGTTAVSNLGMYGITHFTAVINPPHATILAVGTSEERPVVRNGKIEIASMMSVT
CCHHHCCCHHHHHHCCHHHHEEEEEEECCCCEEEEEECCCCCCCEEECCCEEEEEEEEEE
LSCDHRAIDGALGAELIGAFKQLIENPVMMMV
EECCCHHHHHHHHHHHHHHHHHHHCCCCEEEC
>Mature Secondary Structure 
PINILMPALSPTMEKGNLARWLKKEGDQVKSGEVIAEIETDKATMEVEAVDEGTLAKIL
CCEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCEEEEEEECCCCCEEEEE
VPEGTQDVPVNDVIAVLAGEGEDVKAAGSAPATAAPKAEAKPTASAAPAAAPAPAAAPAP
ECCCCCCCCHHHEEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KPAAAPAPSAPAAAAPQVNGHARIFSSPLARRLAKDAGIDLGRITGTGPHGRVVARDVEE
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEHHHHH
AKSGKGLKAAPSAAPAAAGAPAVAPSMSDKQILALFEPGSYEVIPHDGMRRTIAQRLTAA
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCHHHHHHHHHHHHH
TQTVPHFYLTIDCDIGKLLAAREEINAAAPKDKEKKPLYKLSVNDFVIKAMAVALQKIPN
HHCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCC
CNVSWTEGGMVKHKHSDVGVAVAMPGGLITPIIRKAETKTLSAISGEMKDFAARARSRKL
CCEEECCCCEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KPEEYQGGTTAVSNLGMYGITHFTAVINPPHATILAVGTSEERPVVRNGKIEIASMMSVT
CCHHHCCCHHHHHHCCHHHHEEEEEEECCCCEEEEEECCCCCCCEEECCCEEEEEEEEEE
LSCDHRAIDGALGAELIGAFKQLIENPVMMMV
EECCCHHHHHHHHHHHHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]