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The map label for this gene is lpD [H]

Identifier: 146341012

GI number: 146341012

Start: 4272523

End: 4273944

Strand: Reverse

Name: lpD [H]

Synonym: BRADO4083

Alternate gene names: 146341012

Gene position: 4273944-4272523 (Counterclockwise)

Preceding gene: 146341013

Following gene: 146341011

Centisome position: 57.32

GC content: 64.98

Gene sequence:

>1422_bases
ATGGCCGACACATCCTTCGACGTCATCATCATCGGCTCGGGCCCGGGCGGCTACGTCACCGCGATCCGCGCCGCCCAGCT
CGGCTTCAAGACCGCGATCATCGAGAAGACGCATCTCGGCGGCATCTGCCTGAACTGGGGCTGCATCCCGACCAAGGCGC
TCCTACGCTCGGCCGAGATCTATCACTACATGCAGCATGCCAAGGACTACGGTCTCTCTGCTGAGAAGATCTCCTACGAT
CCGAAGGCGGTGGTGCAGCGCTCGCGCGGCGTGTCCAAGCGGCTTGCCGATGGCGTCGGCTTCCTCATGAAGAAGAACAA
GGTGCAGGTGATCTGGGGCCGCGCCAACATCGATGCGCCAGGCAAGATCTCCGTGACCAAGGCCGACGTCGAGGCGCCGA
AGGGCGCGCTGGGCGAGGGGAGCTACCAGGCCAAGCACATCATCGTCGCGACCGGCGCGCGGCCGCGCGTGCTGCCGGGC
CTGGAGCCGGACAAGAAGCTGGTCTGGACCTATTTCGAGGCGATGGTGCCGGACAGGATGCCGAAGTCGCTGCTCGTCGT
CGGCTCCGGCGCGATCGGCATCGAGTTCGCCTCGTTCTTCCGCACCATGGGGTCGGAGGTGACGGTGGTCGAGGTGCTGC
CGCAGATCCTGCCCGTCGAGGACGCCGAGATCGCCGGCATTGCCCGCAAGCAGCTGGAAAAGCAGGGCCTCAAGATCATG
ACCGGCGCCAAGGTCACCAAGCTCGACAAGAAGAGCGACAGCGTGGTGGCGACGATCGACGACGGCAAGGGCAAGATCGA
GACGACCGAGTTCGACCGCGTGATCTCCGCGGTCGGCGTGGTCGGCAACATCGAGAATCTCGGGCTCGAGAAGCTCGGGG
TGAAGACCGACCGCGGCTGCATCGTGATCGACGGCTACGGCAAGACCAACGTGCCCGGCCTCTACGCGATCGGCGACGTC
GCGGGACCGCCGATGCTCGCGCACAAGGCCGAGCATGAGGGCGTGGTCTGCATCGAGGCGATCAAGGGCCTGCATCCGCA
TCCGATGGACAAGAGCCTGATCCCGGGCTGCACCTATTGCCACCCGCAGGTCGCCTCCGTCGGCCTCACCGAAGCCAAGG
CGAAGGAGCAGGGGCGAGACATCCGCGTCGGCCGCTTCCCCTTCGTCGGCAACGGCAAGGCGATCGCGCTCGGCGAGGAC
CAGGGCCTGGTCAAGGTGATCTTCGACAAGAAGACCGGCCAGCTGATCGGCGCGCACATGGTCGGCGCCGAAGTCACCGA
ATTGATCCAGGGCTATGTCGTCGCGATGAACCTCGAGACGACCGAGGAAGAGCTGATGCACACCATCTTCCCGCATCCGA
CGCTGTCGGAGATGATGAAGGAAGCCGTGCTCGACGCCTATGGGCGTGTGCTGAACATGTAG

Upstream 100 bases:

>100_bases
TGCCCACCGACCACATCGCAAGCTCGTGAGACGGTGGGCACGGCGCTCCGCGCCTTTGCCCACCCTACGAGATGACAACT
AAGTTGAGATCGGAGCCGTC

Downstream 100 bases:

>100_bases
TTGCCCCGCCGTCATTGCGAGCGAAGCGAAGCAATCCAGGGGCCCAGGGTGAGACTCTGGATTGCTTCGTCGCTTCGCTC
CTCGCAATGACGAACAACAA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MADTSFDVIIIGSGPGGYVTAIRAAQLGFKTAIIEKTHLGGICLNWGCIPTKALLRSAEIYHYMQHAKDYGLSAEKISYD
PKAVVQRSRGVSKRLADGVGFLMKKNKVQVIWGRANIDAPGKISVTKADVEAPKGALGEGSYQAKHIIVATGARPRVLPG
LEPDKKLVWTYFEAMVPDRMPKSLLVVGSGAIGIEFASFFRTMGSEVTVVEVLPQILPVEDAEIAGIARKQLEKQGLKIM
TGAKVTKLDKKSDSVVATIDDGKGKIETTEFDRVISAVGVVGNIENLGLEKLGVKTDRGCIVIDGYGKTNVPGLYAIGDV
AGPPMLAHKAEHEGVVCIEAIKGLHPHPMDKSLIPGCTYCHPQVASVGLTEAKAKEQGRDIRVGRFPFVGNGKAIALGED
QGLVKVIFDKKTGQLIGAHMVGAEVTELIQGYVVAMNLETTEEELMHTIFPHPTLSEMMKEAVLDAYGRVLNM

Sequences:

>Translated_473_residues
MADTSFDVIIIGSGPGGYVTAIRAAQLGFKTAIIEKTHLGGICLNWGCIPTKALLRSAEIYHYMQHAKDYGLSAEKISYD
PKAVVQRSRGVSKRLADGVGFLMKKNKVQVIWGRANIDAPGKISVTKADVEAPKGALGEGSYQAKHIIVATGARPRVLPG
LEPDKKLVWTYFEAMVPDRMPKSLLVVGSGAIGIEFASFFRTMGSEVTVVEVLPQILPVEDAEIAGIARKQLEKQGLKIM
TGAKVTKLDKKSDSVVATIDDGKGKIETTEFDRVISAVGVVGNIENLGLEKLGVKTDRGCIVIDGYGKTNVPGLYAIGDV
AGPPMLAHKAEHEGVVCIEAIKGLHPHPMDKSLIPGCTYCHPQVASVGLTEAKAKEQGRDIRVGRFPFVGNGKAIALGED
QGLVKVIFDKKTGQLIGAHMVGAEVTELIQGYVVAMNLETTEEELMHTIFPHPTLSEMMKEAVLDAYGRVLNM
>Mature_472_residues
ADTSFDVIIIGSGPGGYVTAIRAAQLGFKTAIIEKTHLGGICLNWGCIPTKALLRSAEIYHYMQHAKDYGLSAEKISYDP
KAVVQRSRGVSKRLADGVGFLMKKNKVQVIWGRANIDAPGKISVTKADVEAPKGALGEGSYQAKHIIVATGARPRVLPGL
EPDKKLVWTYFEAMVPDRMPKSLLVVGSGAIGIEFASFFRTMGSEVTVVEVLPQILPVEDAEIAGIARKQLEKQGLKIMT
GAKVTKLDKKSDSVVATIDDGKGKIETTEFDRVISAVGVVGNIENLGLEKLGVKTDRGCIVIDGYGKTNVPGLYAIGDVA
GPPMLAHKAEHEGVVCIEAIKGLHPHPMDKSLIPGCTYCHPQVASVGLTEAKAKEQGRDIRVGRFPFVGNGKAIALGEDQ
GLVKVIFDKKTGQLIGAHMVGAEVTELIQGYVVAMNLETTEEELMHTIFPHPTLSEMMKEAVLDAYGRVLNM

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=476, Percent_Identity=38.2352941176471, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI50301238, Length=463, Percent_Identity=27.645788336933, Blast_Score=173, Evalue=4e-43,
Organism=Homo sapiens, GI22035672, Length=460, Percent_Identity=27.8260869565217, Blast_Score=136, Evalue=5e-32,
Organism=Homo sapiens, GI291045266, Length=461, Percent_Identity=26.4642082429501, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI33519430, Length=459, Percent_Identity=26.5795206971678, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI33519428, Length=459, Percent_Identity=26.5795206971678, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI33519426, Length=459, Percent_Identity=26.5795206971678, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI148277065, Length=459, Percent_Identity=26.5795206971678, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI148277071, Length=459, Percent_Identity=26.5795206971678, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI291045268, Length=458, Percent_Identity=25.764192139738, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1786307, Length=452, Percent_Identity=35.8407079646018, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI87082354, Length=480, Percent_Identity=27.0833333333333, Blast_Score=168, Evalue=9e-43,
Organism=Escherichia coli, GI1789915, Length=443, Percent_Identity=28.6681715575621, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI87081717, Length=470, Percent_Identity=27.8723404255319, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1789065, Length=207, Percent_Identity=27.536231884058, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=474, Percent_Identity=37.3417721518987, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI17557007, Length=482, Percent_Identity=28.2157676348548, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71983429, Length=462, Percent_Identity=27.2727272727273, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71983419, Length=462, Percent_Identity=27.2727272727273, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71982272, Length=496, Percent_Identity=24.5967741935484, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321091, Length=478, Percent_Identity=39.3305439330544, Blast_Score=295, Evalue=1e-80,
Organism=Saccharomyces cerevisiae, GI6325166, Length=475, Percent_Identity=28.4210526315789, Blast_Score=170, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6325240, Length=474, Percent_Identity=27.2151898734177, Blast_Score=168, Evalue=2e-42,
Organism=Drosophila melanogaster, GI21358499, Length=475, Percent_Identity=39.5789473684211, Blast_Score=323, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24640549, Length=483, Percent_Identity=27.536231884058, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24640553, Length=483, Percent_Identity=27.536231884058, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24640551, Length=483, Percent_Identity=27.536231884058, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI17737741, Length=474, Percent_Identity=25.7383966244726, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50750; Mature: 50619

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTSFDVIIIGSGPGGYVTAIRAAQLGFKTAIIEKTHLGGICLNWGCIPTKALLRSAEI
CCCCCEEEEEEECCCCCHHHHHHHHHHCHHEEEEEECCCCCEEEECCCCCHHHHHHHHHH
YHYMQHAKDYGLSAEKISYDPKAVVQRSRGVSKRLADGVGFLMKKNKVQVIWGRANIDAP
HHHHHHHHHCCCCHHHCCCCHHHHHHHHCCHHHHHHHHHHHEEECCCEEEEEEECCCCCC
GKISVTKADVEAPKGALGEGSYQAKHIIVATGARPRVLPGLEPDKKLVWTYFEAMVPDRM
CEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
PKSLLVVGSGAIGIEFASFFRTMGSEVTVVEVLPQILPVEDAEIAGIARKQLEKQGLKIM
CCEEEEEECCCHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEE
TGAKVTKLDKKSDSVVATIDDGKGKIETTEFDRVISAVGVVGNIENLGLEKLGVKTDRGC
ECCEEEEECCCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHCCHHHCCHHHCCCCCCCCE
IVIDGYGKTNVPGLYAIGDVAGPPMLAHKAEHEGVVCIEAIKGLHPHPMDKSLIPGCTYC
EEEECCCCCCCCCEEEECCCCCCCHHHCCCCCCCEEEEHHHCCCCCCCCCCCCCCCCCCC
HPQVASVGLTEAKAKEQGRDIRVGRFPFVGNGKAIALGEDQGLVKVIFDKKTGQLIGAHM
CCHHHHCCCHHHHHHHCCCCEEEECCCEECCCCEEEEECCCCEEEEEEECCCCCEEEHHH
VGAEVTELIQGYVVAMNLETTEEELMHTIFPHPTLSEMMKEAVLDAYGRVLNM
HHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADTSFDVIIIGSGPGGYVTAIRAAQLGFKTAIIEKTHLGGICLNWGCIPTKALLRSAEI
CCCCEEEEEEECCCCCHHHHHHHHHHCHHEEEEEECCCCCEEEECCCCCHHHHHHHHHH
YHYMQHAKDYGLSAEKISYDPKAVVQRSRGVSKRLADGVGFLMKKNKVQVIWGRANIDAP
HHHHHHHHHCCCCHHHCCCCHHHHHHHHCCHHHHHHHHHHHEEECCCEEEEEEECCCCCC
GKISVTKADVEAPKGALGEGSYQAKHIIVATGARPRVLPGLEPDKKLVWTYFEAMVPDRM
CEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
PKSLLVVGSGAIGIEFASFFRTMGSEVTVVEVLPQILPVEDAEIAGIARKQLEKQGLKIM
CCEEEEEECCCHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEE
TGAKVTKLDKKSDSVVATIDDGKGKIETTEFDRVISAVGVVGNIENLGLEKLGVKTDRGC
ECCEEEEECCCCCCEEEEEECCCCCEEHHHHHHHHHHHHHHCCHHHCCHHHCCCCCCCCE
IVIDGYGKTNVPGLYAIGDVAGPPMLAHKAEHEGVVCIEAIKGLHPHPMDKSLIPGCTYC
EEEECCCCCCCCCEEEECCCCCCCHHHCCCCCCCEEEEHHHCCCCCCCCCCCCCCCCCCC
HPQVASVGLTEAKAKEQGRDIRVGRFPFVGNGKAIALGEDQGLVKVIFDKKTGQLIGAHM
CCHHHHCCCHHHHHHHCCCCEEEECCCEECCCCEEEEECCCCEEEEEEECCCCCEEEHHH
VGAEVTELIQGYVVAMNLETTEEELMHTIFPHPTLSEMMKEAVLDAYGRVLNM
HHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]