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The map label for this gene is ycjZ [C]

Identifier: 146340929

GI number: 146340929

Start: 4191571

End: 4192464

Strand: Reverse

Name: ycjZ [C]

Synonym: BRADO4000

Alternate gene names: 146340929

Gene position: 4192464-4191571 (Counterclockwise)

Preceding gene: 146340931

Following gene: 146340928

Centisome position: 56.22

GC content: 66.67

Gene sequence:

>894_bases
ATGCTGCGCGAGAACCTGAACGACGTGCTCGGTTTCCTCGCCGTCGCCCGCGAGGGAAGCTTCACGCGGGCGGCTGCCCG
GCTCGGCATCAGCCAGTCGGCGCTCAGCCATTCCCTTCGCAACCTCGAGGCCCGGCTCGGCGTGCGGCTGCTGGCGCGCA
CGACGCGCAGCGTCGCGCCGACGGCTGCAGGCGAACGCCTGCTGCGGAGCATCGGGCCGCGGTTCGATGAGATCGAAACG
GAACTGGCCGGCTTGAGCGAATTGCGCGACAAGCCGGCCGGAACGATCCGGATCACGGCGGGCGAGCATGCCGCCGACTC
CGTGCTCTGGCCGGCGCTGAGAAAGCTGCTTCCGGTCTATCCGGACATCAAGGTTGAGGTCGTGACCGACTATGGGCTGA
CCGACATCGTCGCCGAGAGATTCGACGCGGGGGTGCGGCTCGGCGAGCAGGTCGCCAAGGACATGATCGCCGTCAGGATC
GGGCCGGACCTGCGCATGGCCGTGGTGGGCTCACCGGCCTATTTCGCCGTGCGGGCGCGGCCGCGGAGGCCGCAGGATCT
CACGGAGCACAATTGCATCAACATCCGGCTTCCGACCCTGGGAGGGATCTATGCGTGGGAGTTCGAAAAGCGCGGACGCA
CGCTGAAGGTGCGCGTCGACGGGCAACTGGTGTTCAACAACATCACGATGCGAATGAATGCGGTGCGCGCCGGGCTCGGG
CTCGCTTATCTGCCCGAGGATCAGGTCCGGGACGATCTTGCGAAGGGCAAACTCGTTCGCGTCCTGGAGGACTGGTGCGC
GCCGTTTGCCGGCTATCATCTGTATTTTCCGAGCCGCCGGCAGCCCACATCTGCGTTCACGGTGCTGGTCGACGCGCTTC
GCTGTCGCGGATGA

Upstream 100 bases:

>100_bases
CCGCGCATCGTCGACCTCTCCGTCCAGACTGAACCTAAGGCTGTGACTACGATGACATTAGATGCTAGAACGCGCATGGT
TTTATGAAATGAGCTCATGA

Downstream 100 bases:

>100_bases
CGAGCCCGGTTCGCGCGATCGTCTCGCCGGACAAACGGATTTTGCTGCAAAATCAACGGCTCCCGTGCGCCCCCGCAAGG
CCTCTCTGCAACCGCTGCGC

Product: OprD regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MLRENLNDVLGFLAVAREGSFTRAAARLGISQSALSHSLRNLEARLGVRLLARTTRSVAPTAAGERLLRSIGPRFDEIET
ELAGLSELRDKPAGTIRITAGEHAADSVLWPALRKLLPVYPDIKVEVVTDYGLTDIVAERFDAGVRLGEQVAKDMIAVRI
GPDLRMAVVGSPAYFAVRARPRRPQDLTEHNCINIRLPTLGGIYAWEFEKRGRTLKVRVDGQLVFNNITMRMNAVRAGLG
LAYLPEDQVRDDLAKGKLVRVLEDWCAPFAGYHLYFPSRRQPTSAFTVLVDALRCRG

Sequences:

>Translated_297_residues
MLRENLNDVLGFLAVAREGSFTRAAARLGISQSALSHSLRNLEARLGVRLLARTTRSVAPTAAGERLLRSIGPRFDEIET
ELAGLSELRDKPAGTIRITAGEHAADSVLWPALRKLLPVYPDIKVEVVTDYGLTDIVAERFDAGVRLGEQVAKDMIAVRI
GPDLRMAVVGSPAYFAVRARPRRPQDLTEHNCINIRLPTLGGIYAWEFEKRGRTLKVRVDGQLVFNNITMRMNAVRAGLG
LAYLPEDQVRDDLAKGKLVRVLEDWCAPFAGYHLYFPSRRQPTSAFTVLVDALRCRG
>Mature_297_residues
MLRENLNDVLGFLAVAREGSFTRAAARLGISQSALSHSLRNLEARLGVRLLARTTRSVAPTAAGERLLRSIGPRFDEIET
ELAGLSELRDKPAGTIRITAGEHAADSVLWPALRKLLPVYPDIKVEVVTDYGLTDIVAERFDAGVRLGEQVAKDMIAVRI
GPDLRMAVVGSPAYFAVRARPRRPQDLTEHNCINIRLPTLGGIYAWEFEKRGRTLKVRVDGQLVFNNITMRMNAVRAGLG
LAYLPEDQVRDDLAKGKLVRVLEDWCAPFAGYHLYFPSRRQPTSAFTVLVDALRCRG

Specific function: May have a role in the regulation of oprD expression [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787589, Length=297, Percent_Identity=51.5151515151515, Blast_Score=318, Evalue=3e-88,
Organism=Escherichia coli, GI1787128, Length=293, Percent_Identity=41.296928327645, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI1786401, Length=264, Percent_Identity=27.6515151515151, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI87081978, Length=298, Percent_Identity=29.8657718120805, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1789639, Length=290, Percent_Identity=28.2758620689655, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI145693193, Length=276, Percent_Identity=30.7971014492754, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1789440, Length=260, Percent_Identity=25.7692307692308, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786448, Length=286, Percent_Identity=26.9230769230769, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788748, Length=120, Percent_Identity=36.6666666666667, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI145693105, Length=156, Percent_Identity=30.7692307692308, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787879, Length=87, Percent_Identity=45.9770114942529, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789173, Length=260, Percent_Identity=25.3846153846154, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI157672245, Length=148, Percent_Identity=31.0810810810811, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32914; Mature: 32914

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRENLNDVLGFLAVAREGSFTRAAARLGISQSALSHSLRNLEARLGVRLLARTTRSVAP
CCCCHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TAAGERLLRSIGPRFDEIETELAGLSELRDKPAGTIRITAGEHAADSVLWPALRKLLPVY
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCC
PDIKVEVVTDYGLTDIVAERFDAGVRLGEQVAKDMIAVRIGPDLRMAVVGSPAYFAVRAR
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEEECCCEEEEEECC
PRRPQDLTEHNCINIRLPTLGGIYAWEFEKRGRTLKVRVDGQLVFNNITMRMNAVRAGLG
CCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCEEEEEECCEEEEEHHHHHHHHHHHCCC
LAYLPEDQVRDDLAKGKLVRVLEDWCAPFAGYHLYFPSRRQPTSAFTVLVDALRCRG
EEECCCHHHHHHHHHHHHHHHHHHHCCHHCCEEEECCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLRENLNDVLGFLAVAREGSFTRAAARLGISQSALSHSLRNLEARLGVRLLARTTRSVAP
CCCCHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TAAGERLLRSIGPRFDEIETELAGLSELRDKPAGTIRITAGEHAADSVLWPALRKLLPVY
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCC
PDIKVEVVTDYGLTDIVAERFDAGVRLGEQVAKDMIAVRIGPDLRMAVVGSPAYFAVRAR
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEEECCCEEEEEECC
PRRPQDLTEHNCINIRLPTLGGIYAWEFEKRGRTLKVRVDGQLVFNNITMRMNAVRAGLG
CCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCEEEEEECCEEEEEHHHHHHHHHHHCCC
LAYLPEDQVRDDLAKGKLVRVLEDWCAPFAGYHLYFPSRRQPTSAFTVLVDALRCRG
EEECCCHHHHHHHHHHHHHHHHHHHCCHHCCEEEECCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043; 1427017 [H]