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The map label for this gene is alkK [H]

Identifier: 146340775

GI number: 146340775

Start: 4004125

End: 4005768

Strand: Reverse

Name: alkK [H]

Synonym: BRADO3833

Alternate gene names: 146340775

Gene position: 4005768-4004125 (Counterclockwise)

Preceding gene: 146340776

Following gene: 146340767

Centisome position: 53.72

GC content: 65.51

Gene sequence:

>1644_bases
ATGAGCACGAACCAGTACCAGCTCGGCCTCGACAAGAACCCCGCCAATTACGTGCCGCTGACCCCGCTCAGCTTCCTGGC
GCGCAGCGCGGCCGTCTACCCCGATCACGTCAGCACGGTCTACGAGGGTCGCAGCTTCACCTGGCGCCAGACCTATGAGC
GCTGCCGCCGCTTCGCGTCCTATCTCGCCGGCCGCGGCATCGGCACCGGCGACACCGTGGCGGCGATGCTGCCGAACATC
CCGGCGATGAACGAGGCGCATTTCGCCGTGCCGATGACAGGCGCGGTGCTCAACACGCTCAACATCCGCCTCGATGCGCC
GTCGATCGCGTTCCAGCTTGATCACGCCCAGGCCAAGATCATCCTGGTCGATCCGGAATTCTCCGGCGTGATCAGCGAGG
CGCTGAAGCTGATGAGCGGCGCAAAGCCGTTCGTGATCGACGTCGATGACGCGTCCTACGCCGGCGGCAGCCGTATCGGC
GAAATCGAGTATGAGGCCGCCGTCGCCACTGGTGATCCCTCCTTCGAGCCGCGCCGTCCCACTGATGAGTGGGACGCGAT
TGCGATGAGCTACACCTCGGGCACGACCGGCAATCCCAAGGGCGTCGTGACGCATCATCGCGGCGCCTATCTTAACGCGG
TCAGCAACATCCTCGCCGGCGGGCTCGGCCAGCATCCGGTCTATCTCTGGACCCTGCCGATGTTCCACTGCAACGGCTGG
TGCTTTCCCTGGACCATGGCGGCGGCGGCTGGCGTCAACGTCTGCCTGCGCAAGGTCGAGCCGACGAAGATCTTCGAGCT
GATCAAGACCCACGGCGTCACCCATATGTGCGGCGCACCGATCGTCTACAACGTGCTGATCAATGCTCCGGACGCGCCCA
AGGGCAAGCGCGACAGACCGGTGGTCGGCCTGATCGCCGGCGCCGCGCCGCCGGTCGCCGTGCTCGAAGGCGCCGAGAGC
ATCGGCATCAAGCTGACCCATGTCTACGGCCTCACCGAGGTCTACGGCCCCGCCTCCGTCTGCGCCGAGCAGCCCGGCTG
GGACGATTTGCCGGCGCCCGAGCGCGCGCGGATGAAGCGCCGCCAGGGCGTGCCCTATCCGCTGCAGGAAGCCGTCACCG
TGCTGAATCCGGAGACGATGCAGGAGGTGCCGCGCGACGGCGAGACCATCGGCGAGGTCATGTTCCGCGGCAACATCGTC
ATGAAGGGCTATCTCAAGAACGAGAAGGCCACGCAGGACGCCTTCGCCGGCGGCTGGTTTCACACCGGCGATCTCGGCGT
GCTCGACGAGCACGGCTACGTCATCATCAAGGACCGTTCCAAGGACATCATCATCTCCGGCGGCGAGAACATCTCCTCGG
TCGAGGTCGAGGACATCCTCTACAAGCACCCCGCCGTGCTGTTCGCCGCCGTCGTCGCCAAGCCCGACCCGAAATGGGGC
GAGGTCCCCTGCGCCTTCATCGAGCTGAAGGACGGCGCCCAGGCCAAGGAAGCCGAGATCATCGCCTATTGCCGCAGCCA
CATGTCCGGCTTCAAGACGCCGAAGGTCGTGGTGTTCGGCCCGATCCCGAAGACGTCGACGGGCAAGATCCAGAAGTTCC
TGCTGAGGAACGAGGTAAACTCGGCGAAGGCGATTTCGGCGTAG

Upstream 100 bases:

>100_bases
TACCTGCTGGCGCTTCTCATAGGCCTCATCATCGTTGCGATTTTTCCATGGATTTCGACCGGGTTCCTGTAAACTCTTGA
GATGACAAGGGAGGGCAGCC

Downstream 100 bases:

>100_bases
CCACACGAGTAGCGCGGGTTAGCCGCAGGCGTAACCCGCCGGCCCCTGGGGACATGGCGGATTACGCCGCGCTGATCCGC
CCTACGCACTTCGCATCGTA

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MSTNQYQLGLDKNPANYVPLTPLSFLARSAAVYPDHVSTVYEGRSFTWRQTYERCRRFASYLAGRGIGTGDTVAAMLPNI
PAMNEAHFAVPMTGAVLNTLNIRLDAPSIAFQLDHAQAKIILVDPEFSGVISEALKLMSGAKPFVIDVDDASYAGGSRIG
EIEYEAAVATGDPSFEPRRPTDEWDAIAMSYTSGTTGNPKGVVTHHRGAYLNAVSNILAGGLGQHPVYLWTLPMFHCNGW
CFPWTMAAAAGVNVCLRKVEPTKIFELIKTHGVTHMCGAPIVYNVLINAPDAPKGKRDRPVVGLIAGAAPPVAVLEGAES
IGIKLTHVYGLTEVYGPASVCAEQPGWDDLPAPERARMKRRQGVPYPLQEAVTVLNPETMQEVPRDGETIGEVMFRGNIV
MKGYLKNEKATQDAFAGGWFHTGDLGVLDEHGYVIIKDRSKDIIISGGENISSVEVEDILYKHPAVLFAAVVAKPDPKWG
EVPCAFIELKDGAQAKEAEIIAYCRSHMSGFKTPKVVVFGPIPKTSTGKIQKFLLRNEVNSAKAISA

Sequences:

>Translated_547_residues
MSTNQYQLGLDKNPANYVPLTPLSFLARSAAVYPDHVSTVYEGRSFTWRQTYERCRRFASYLAGRGIGTGDTVAAMLPNI
PAMNEAHFAVPMTGAVLNTLNIRLDAPSIAFQLDHAQAKIILVDPEFSGVISEALKLMSGAKPFVIDVDDASYAGGSRIG
EIEYEAAVATGDPSFEPRRPTDEWDAIAMSYTSGTTGNPKGVVTHHRGAYLNAVSNILAGGLGQHPVYLWTLPMFHCNGW
CFPWTMAAAAGVNVCLRKVEPTKIFELIKTHGVTHMCGAPIVYNVLINAPDAPKGKRDRPVVGLIAGAAPPVAVLEGAES
IGIKLTHVYGLTEVYGPASVCAEQPGWDDLPAPERARMKRRQGVPYPLQEAVTVLNPETMQEVPRDGETIGEVMFRGNIV
MKGYLKNEKATQDAFAGGWFHTGDLGVLDEHGYVIIKDRSKDIIISGGENISSVEVEDILYKHPAVLFAAVVAKPDPKWG
EVPCAFIELKDGAQAKEAEIIAYCRSHMSGFKTPKVVVFGPIPKTSTGKIQKFLLRNEVNSAKAISA
>Mature_546_residues
STNQYQLGLDKNPANYVPLTPLSFLARSAAVYPDHVSTVYEGRSFTWRQTYERCRRFASYLAGRGIGTGDTVAAMLPNIP
AMNEAHFAVPMTGAVLNTLNIRLDAPSIAFQLDHAQAKIILVDPEFSGVISEALKLMSGAKPFVIDVDDASYAGGSRIGE
IEYEAAVATGDPSFEPRRPTDEWDAIAMSYTSGTTGNPKGVVTHHRGAYLNAVSNILAGGLGQHPVYLWTLPMFHCNGWC
FPWTMAAAAGVNVCLRKVEPTKIFELIKTHGVTHMCGAPIVYNVLINAPDAPKGKRDRPVVGLIAGAAPPVAVLEGAESI
GIKLTHVYGLTEVYGPASVCAEQPGWDDLPAPERARMKRRQGVPYPLQEAVTVLNPETMQEVPRDGETIGEVMFRGNIVM
KGYLKNEKATQDAFAGGWFHTGDLGVLDEHGYVIIKDRSKDIIISGGENISSVEVEDILYKHPAVLFAAVVAKPDPKWGE
VPCAFIELKDGAQAKEAEIIAYCRSHMSGFKTPKVVVFGPIPKTSTGKIQKFLLRNEVNSAKAISA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=535, Percent_Identity=29.7196261682243, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI187761345, Length=547, Percent_Identity=25.5941499085923, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI187761343, Length=547, Percent_Identity=25.5941499085923, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI157311624, Length=524, Percent_Identity=28.4351145038168, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI157311622, Length=524, Percent_Identity=28.4351145038168, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI58082049, Length=524, Percent_Identity=27.8625954198473, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI122937307, Length=500, Percent_Identity=27.2, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI38505220, Length=521, Percent_Identity=26.8714011516315, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI42544132, Length=505, Percent_Identity=26.5346534653465, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI115511026, Length=521, Percent_Identity=26.4875239923225, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI13375727, Length=559, Percent_Identity=25.0447227191413, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI28416953, Length=557, Percent_Identity=24.2369838420108, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI40807357, Length=553, Percent_Identity=25.1356238698011, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI8923896, Length=564, Percent_Identity=24.468085106383, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI253970489, Length=375, Percent_Identity=26.4, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1788107, Length=561, Percent_Identity=28.1639928698752, Blast_Score=172, Evalue=6e-44,
Organism=Escherichia coli, GI145693145, Length=526, Percent_Identity=28.8973384030418, Blast_Score=166, Evalue=5e-42,
Organism=Escherichia coli, GI221142682, Length=493, Percent_Identity=25.9634888438134, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1786810, Length=542, Percent_Identity=26.9372693726937, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1788595, Length=506, Percent_Identity=25.296442687747, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1786529, Length=546, Percent_Identity=24.9084249084249, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71994694, Length=530, Percent_Identity=27.1698113207547, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI71994703, Length=530, Percent_Identity=27.1698113207547, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI71994690, Length=530, Percent_Identity=27.1698113207547, Blast_Score=177, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17558820, Length=513, Percent_Identity=27.0955165692008, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17559526, Length=519, Percent_Identity=26.2042389210019, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17560308, Length=521, Percent_Identity=25.9117082533589, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32563687, Length=504, Percent_Identity=26.3888888888889, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17538037, Length=517, Percent_Identity=25.9187620889749, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17557194, Length=540, Percent_Identity=25.5555555555556, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17531443, Length=535, Percent_Identity=22.0560747663551, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71983001, Length=555, Percent_Identity=23.6036036036036, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI71982997, Length=555, Percent_Identity=23.6036036036036, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17560140, Length=500, Percent_Identity=23.6, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71985884, Length=398, Percent_Identity=28.1407035175879, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI71996755, Length=520, Percent_Identity=23.6538461538462, Blast_Score=92, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6319699, Length=361, Percent_Identity=27.7008310249307, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6323182, Length=561, Percent_Identity=23.1729055258467, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6319264, Length=571, Percent_Identity=22.9422066549912, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24581924, Length=554, Percent_Identity=26.3537906137184, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI18859661, Length=531, Percent_Identity=26.5536723163842, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI21355181, Length=534, Percent_Identity=27.3408239700375, Blast_Score=129, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24656500, Length=543, Percent_Identity=24.6777163904236, Blast_Score=102, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24667955, Length=565, Percent_Identity=23.8938053097345, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI62472339, Length=560, Percent_Identity=24.1071428571429, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24583465, Length=459, Percent_Identity=24.6187363834423, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24583463, Length=459, Percent_Identity=24.6187363834423, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281364798, Length=459, Percent_Identity=24.6187363834423, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI281365686, Length=534, Percent_Identity=21.9101123595506, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI21358303, Length=487, Percent_Identity=22.5872689938398, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24667959, Length=378, Percent_Identity=25.9259259259259, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI21356947, Length=547, Percent_Identity=23.0347349177331, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI20130357, Length=377, Percent_Identity=23.8726790450928, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648260, Length=518, Percent_Identity=22.3938223938224, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17933690, Length=163, Percent_Identity=28.2208588957055, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 59206; Mature: 59075

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTNQYQLGLDKNPANYVPLTPLSFLARSAAVYPDHVSTVYEGRSFTWRQTYERCRRFAS
CCCCCEEECCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHH
YLAGRGIGTGDTVAAMLPNIPAMNEAHFAVPMTGAVLNTLNIRLDAPSIAFQLDHAQAKI
HHHCCCCCCCCHHHHHCCCCCCCCCCEEEEEEHHHEEEEEEEEECCCCEEEEEECCCEEE
ILVDPEFSGVISEALKLMSGAKPFVIDVDDASYAGGSRIGEIEYEAAVATGDPSFEPRRP
EEECCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEECEEEEEEEEECCCCCCCCCCC
TDEWDAIAMSYTSGTTGNPKGVVTHHRGAYLNAVSNILAGGLGQHPVYLWTLPMFHCNGW
CCCCCEEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCEEEEEEEHHHCCCC
CFPWTMAAAAGVNVCLRKVEPTKIFELIKTHGVTHMCGAPIVYNVLINAPDAPKGKRDRP
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHCCCCEEEEEEEECCCCCCCCCCCC
VVGLIAGAAPPVAVLEGAESIGIKLTHVYGLTEVYGPASVCAEQPGWDDLPAPERARMKR
EEEEEECCCCCHHHHCCCHHCCEEEEEEECHHHHCCCHHHHCCCCCCCCCCCCHHHHHHH
RQGVPYPLQEAVTVLNPETMQEVPRDGETIGEVMFRGNIVMKGYLKNEKATQDAFAGGWF
HCCCCCCHHHHHHHCCCHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCCCCHHHHCCCCE
HTGDLGVLDEHGYVIIKDRSKDIIISGGENISSVEVEDILYKHPAVLFAAVVAKPDPKWG
ECCCCCEEECCCEEEEEECCCEEEEECCCCCCCEEHHHHHHHCHHHHHHHHHCCCCCCCC
EVPCAFIELKDGAQAKEAEIIAYCRSHMSGFKTPKVVVFGPIPKTSTGKIQKFLLRNEVN
CCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCC
SAKAISA
CCCCCCC
>Mature Secondary Structure 
STNQYQLGLDKNPANYVPLTPLSFLARSAAVYPDHVSTVYEGRSFTWRQTYERCRRFAS
CCCCEEECCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHH
YLAGRGIGTGDTVAAMLPNIPAMNEAHFAVPMTGAVLNTLNIRLDAPSIAFQLDHAQAKI
HHHCCCCCCCCHHHHHCCCCCCCCCCEEEEEEHHHEEEEEEEEECCCCEEEEEECCCEEE
ILVDPEFSGVISEALKLMSGAKPFVIDVDDASYAGGSRIGEIEYEAAVATGDPSFEPRRP
EEECCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEECEEEEEEEEECCCCCCCCCCC
TDEWDAIAMSYTSGTTGNPKGVVTHHRGAYLNAVSNILAGGLGQHPVYLWTLPMFHCNGW
CCCCCEEEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCEEEEEEEHHHCCCC
CFPWTMAAAAGVNVCLRKVEPTKIFELIKTHGVTHMCGAPIVYNVLINAPDAPKGKRDRP
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHCCCCEEEEEEEECCCCCCCCCCCC
VVGLIAGAAPPVAVLEGAESIGIKLTHVYGLTEVYGPASVCAEQPGWDDLPAPERARMKR
EEEEEECCCCCHHHHCCCHHCCEEEEEEECHHHHCCCHHHHCCCCCCCCCCCCHHHHHHH
RQGVPYPLQEAVTVLNPETMQEVPRDGETIGEVMFRGNIVMKGYLKNEKATQDAFAGGWF
HCCCCCCHHHHHHHCCCHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCCCCHHHHCCCCE
HTGDLGVLDEHGYVIIKDRSKDIIISGGENISSVEVEDILYKHPAVLFAAVVAKPDPKWG
ECCCCCEEECCCEEEEEECCCEEEEECCCCCCCEEHHHHHHHCHHHHHHHHHCCCCCCCC
EVPCAFIELKDGAQAKEAEIIAYCRSHMSGFKTPKVVVFGPIPKTSTGKIQKFLLRNEVN
CCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCC
SAKAISA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]