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The map label for this gene is metG [H]

Identifier: 146340771

GI number: 146340771

Start: 3999416

End: 4001533

Strand: Direct

Name: metG [H]

Synonym: BRADO3829

Alternate gene names: 146340771

Gene position: 3999416-4001533 (Clockwise)

Preceding gene: 146340770

Following gene: 146340772

Centisome position: 53.64

GC content: 66.57

Gene sequence:

>2118_bases
GTGGCCAAGGCCAGCAAGACAACCGTGAAGAAGGGCAAGCCGGCGACCAAGGGCGCCGCGACGAAGGCTGCCGCCAAGAA
GGCTTCGAAGTCGCCCGCGAAGGCCAAAGCCGGCGCGACCAAGGCGAAGGCCGCGGCGGACAAGGACAAGAAGGCCAAGG
CCGCCAAAGCCAATGCCGCCAAAGCCAAGGCCACGAAGGGCAAAGCGTCGTCCGCGCCGGCCAAGGGCAAGTCACCAAAG
GCTGCCAAGAAGGCCGCGAAGTCGGCGACCAAGCCCGCTGTACCGGCCAAGAAGACTGCGGCCAAAGCCGCCGCCACGAA
GCCCGCGCCATCGAAGAAGCCAGCCGAGACGAAGCCGGCGCCTGCTCCGAAGCTAGTTGCGCCAGCGGTCGCCGAAAGCC
CGAAGCCGCCGCCGCGCGCCGCAAAGCCAAAGACCCCGCGCAAGCGGAAGGTCGAGGCTGCCGCGGCTCCGGAGATCGAG
ATCGCCGAGATCGAAGTGGCTGAAGTCGAGATCGTCGAGATCGAGGCGACCGATACCGCGGAGGACGCGACGCCCGTCGC
GGCCGCGACTGAAAACGCCAACACCTTCTACGTCACGACCGCGATCGCCTATCCCAATGGCAGCCCGCATATCGGCCACG
CCTATGAGGCCATCGCGACCGATGCCATCGCGCGCTTCGCGCGGCTCGACGGCAAGGACGTGTTCTTCCTGACCGGCACC
GACGAGCACGGCCTGAAGATGGTGCAGACCGCGCAGAACGAGGGCCTGACACCGTCGGAGCTCGCGGCCCGCAATGCCGG
CCGCTTCCGCGAGATGGACGAGCGGCTGAACGTCTCGTTCGACCGCTTCATCCGCACCACCGAGCCCGCCCACCACAAAT
CGGTGCAGGCGATCTGGACGCGCATGCTCGAGAACGGCGATATCTACGCCGACACCTATTCGGGCTGGTACTCGGTGCGC
GACGAAGCGTACTACGCCGAGGAGGAGACGGTCGTCGGCGAGGACAATGTCCGCCGCGGGCCGCAGGGCACGCCGGTCGA
GTGGGTCGAGGAGAAGAGCTACTTCTTCCGCCTGTCGGCCTATCAGGACAAGCTGCTCGCGCTGTACGAGAACCAGCCCG
ATTTCATCGGGCCGGATGCGCGGCGCAACGAGATCACGAGCTTCGTCCGGGGCGGCCTGAAGGACCTCTCGATCTCGCGC
ACGACGTTCGACTGGGGTGTCCGCGTGCCGCATGACGACGAGCACGTGATGTATGTCTGGGTCGACGCGCTGACCAACTA
CATCACCGGCGTCGGCTTCCCCGATGAGAATGATGCGAACTGGAAGTTCTGGCCGGCCGACGTGCACGTCATCGGCAAGG
ACATCATCCGATTCCACGCGGTGTACTGGCCGGCGTTCCTGATGTCGGCCGGCGTGCCGGTGCCGAAGCGGGTCTATGCC
CACGGCTTCCTGTTCAACAGGGGCGAGAAGATGTCGAAGTCGGTCGGCAATGTGGTCGATCCGTTCAACCTCGCCGATCA
GTACGGCGTGGACCAGCTGCGCTATTTCTTCCTGCGCGAGGTGCCGTTCGGCCAGGACGGCAACTACAATCACGAGGCGA
TCGTCGCGCGCATCAACGCCGATCTCGCCAACGACCTCGGCAATCTCGCGCAGCGCTCGCTGTCGATGATCACCAAGCAG
TTCGGCGGCGTGCTGCCGGAGCCCGGCGAGTTTTCGGCCAACGATCAAGCGATCCTGGCCGAGGCCGATGCGATGATCGG
CGCGGCGCGCAAGGCGATGGCGACCCAGCAGATCCACCAATGGCTCAATGCCGTGTGGTCGGTGGTGGCCGAGGCCAACC
GCTACTTTGCGGGCGAGGCGCCGTGGGCGCTGGCCAAGACCGATCCGAAGCGCCAGCACACCGTGCTCTATGTCACCGCC
GAGGTGATCCGGCAGGTCGCGATCCTGACTCAGCCGGTGATGCCCGCCGCGTCGGCGAAGCTGCTCGACAGCCTGGGAAT
CCCGGAGGACGAGCGGAGCTTCGCGCGGCTCGGCGGCGAGGTGCGGATCGCACCGGGCACGCAGCTGCCAGCGCCGCAGG
CGGTGTTCCCGCGCTACATCGAGCCGAGCGCGGCGTGA

Upstream 100 bases:

>100_bases
GTTCGGGATGCTCGCCGAAGCCACGCGGTAACGCGCCGACCATCATCCCGGGATCATCAGGATCAATCCGTCCACCGACG
GCGTCAAAGAGGAATTTGCC

Downstream 100 bases:

>100_bases
TGCTGGTCGACTCTCATTGCCATCTGGATTTTCCGGATTTTGCCGAGGATCTGGATGGCATCGTCACGCGCGCCGCCGCT
GCCGGCGTCGGCCGCCTGGT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 705; Mature: 704

Protein sequence:

>705_residues
MAKASKTTVKKGKPATKGAATKAAAKKASKSPAKAKAGATKAKAAADKDKKAKAAKANAAKAKATKGKASSAPAKGKSPK
AAKKAAKSATKPAVPAKKTAAKAAATKPAPSKKPAETKPAPAPKLVAPAVAESPKPPPRAAKPKTPRKRKVEAAAAPEIE
IAEIEVAEVEIVEIEATDTAEDATPVAAATENANTFYVTTAIAYPNGSPHIGHAYEAIATDAIARFARLDGKDVFFLTGT
DEHGLKMVQTAQNEGLTPSELAARNAGRFREMDERLNVSFDRFIRTTEPAHHKSVQAIWTRMLENGDIYADTYSGWYSVR
DEAYYAEEETVVGEDNVRRGPQGTPVEWVEEKSYFFRLSAYQDKLLALYENQPDFIGPDARRNEITSFVRGGLKDLSISR
TTFDWGVRVPHDDEHVMYVWVDALTNYITGVGFPDENDANWKFWPADVHVIGKDIIRFHAVYWPAFLMSAGVPVPKRVYA
HGFLFNRGEKMSKSVGNVVDPFNLADQYGVDQLRYFFLREVPFGQDGNYNHEAIVARINADLANDLGNLAQRSLSMITKQ
FGGVLPEPGEFSANDQAILAEADAMIGAARKAMATQQIHQWLNAVWSVVAEANRYFAGEAPWALAKTDPKRQHTVLYVTA
EVIRQVAILTQPVMPAASAKLLDSLGIPEDERSFARLGGEVRIAPGTQLPAPQAVFPRYIEPSAA

Sequences:

>Translated_705_residues
MAKASKTTVKKGKPATKGAATKAAAKKASKSPAKAKAGATKAKAAADKDKKAKAAKANAAKAKATKGKASSAPAKGKSPK
AAKKAAKSATKPAVPAKKTAAKAAATKPAPSKKPAETKPAPAPKLVAPAVAESPKPPPRAAKPKTPRKRKVEAAAAPEIE
IAEIEVAEVEIVEIEATDTAEDATPVAAATENANTFYVTTAIAYPNGSPHIGHAYEAIATDAIARFARLDGKDVFFLTGT
DEHGLKMVQTAQNEGLTPSELAARNAGRFREMDERLNVSFDRFIRTTEPAHHKSVQAIWTRMLENGDIYADTYSGWYSVR
DEAYYAEEETVVGEDNVRRGPQGTPVEWVEEKSYFFRLSAYQDKLLALYENQPDFIGPDARRNEITSFVRGGLKDLSISR
TTFDWGVRVPHDDEHVMYVWVDALTNYITGVGFPDENDANWKFWPADVHVIGKDIIRFHAVYWPAFLMSAGVPVPKRVYA
HGFLFNRGEKMSKSVGNVVDPFNLADQYGVDQLRYFFLREVPFGQDGNYNHEAIVARINADLANDLGNLAQRSLSMITKQ
FGGVLPEPGEFSANDQAILAEADAMIGAARKAMATQQIHQWLNAVWSVVAEANRYFAGEAPWALAKTDPKRQHTVLYVTA
EVIRQVAILTQPVMPAASAKLLDSLGIPEDERSFARLGGEVRIAPGTQLPAPQAVFPRYIEPSAA
>Mature_704_residues
AKASKTTVKKGKPATKGAATKAAAKKASKSPAKAKAGATKAKAAADKDKKAKAAKANAAKAKATKGKASSAPAKGKSPKA
AKKAAKSATKPAVPAKKTAAKAAATKPAPSKKPAETKPAPAPKLVAPAVAESPKPPPRAAKPKTPRKRKVEAAAAPEIEI
AEIEVAEVEIVEIEATDTAEDATPVAAATENANTFYVTTAIAYPNGSPHIGHAYEAIATDAIARFARLDGKDVFFLTGTD
EHGLKMVQTAQNEGLTPSELAARNAGRFREMDERLNVSFDRFIRTTEPAHHKSVQAIWTRMLENGDIYADTYSGWYSVRD
EAYYAEEETVVGEDNVRRGPQGTPVEWVEEKSYFFRLSAYQDKLLALYENQPDFIGPDARRNEITSFVRGGLKDLSISRT
TFDWGVRVPHDDEHVMYVWVDALTNYITGVGFPDENDANWKFWPADVHVIGKDIIRFHAVYWPAFLMSAGVPVPKRVYAH
GFLFNRGEKMSKSVGNVVDPFNLADQYGVDQLRYFFLREVPFGQDGNYNHEAIVARINADLANDLGNLAQRSLSMITKQF
GGVLPEPGEFSANDQAILAEADAMIGAARKAMATQQIHQWLNAVWSVVAEANRYFAGEAPWALAKTDPKRQHTVLYVTAE
VIRQVAILTQPVMPAASAKLLDSLGIPEDERSFARLGGEVRIAPGTQLPAPQAVFPRYIEPSAA

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=511, Percent_Identity=37.3776908023483, Blast_Score=334, Evalue=2e-91,
Organism=Homo sapiens, GI14043022, Length=603, Percent_Identity=26.3681592039801, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI7661872, Length=200, Percent_Identity=27, Blast_Score=75, Evalue=3e-13,
Organism=Escherichia coli, GI1788432, Length=510, Percent_Identity=26.8627450980392, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1786861, Length=178, Percent_Identity=26.9662921348315, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI212645096, Length=517, Percent_Identity=36.3636363636364, Blast_Score=327, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI71992781, Length=411, Percent_Identity=34.7931873479319, Blast_Score=243, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI17540794, Length=523, Percent_Identity=27.5334608030593, Blast_Score=171, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6321610, Length=535, Percent_Identity=32.8971962616822, Blast_Score=306, Evalue=5e-84,
Organism=Saccharomyces cerevisiae, GI6321704, Length=536, Percent_Identity=24.4402985074627, Blast_Score=121, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6323414, Length=178, Percent_Identity=25.8426966292135, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI21358483, Length=523, Percent_Identity=35.5640535372849, Blast_Score=315, Evalue=5e-86,
Organism=Drosophila melanogaster, GI24655311, Length=517, Percent_Identity=25.531914893617, Blast_Score=137, Evalue=3e-32,
Organism=Drosophila melanogaster, GI21355409, Length=179, Percent_Identity=29.608938547486, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 76417; Mature: 76286

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKASKTTVKKGKPATKGAATKAAAKKASKSPAKAKAGATKAKAAADKDKKAKAAKANAA
CCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCHHHCCCCHHHHHCCCHHHHHHHHCCHH
KAKATKGKASSAPAKGKSPKAAKKAAKSATKPAVPAKKTAAKAAATKPAPSKKPAETKPA
HHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCC
PAPKLVAPAVAESPKPPPRAAKPKTPRKRKVEAAAAPEIEIAEIEVAEVEIVEIEATDTA
CCCCHHCCHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEEEEEEEEEEEECCCCC
EDATPVAAATENANTFYVTTAIAYPNGSPHIGHAYEAIATDAIARFARLDGKDVFFLTGT
CCCCCCEEECCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
DEHGLKMVQTAQNEGLTPSELAARNAGRFREMDERLNVSFDRFIRTTEPAHHKSVQAIWT
CCCCHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHH
RMLENGDIYADTYSGWYSVRDEAYYAEEETVVGEDNVRRGPQGTPVEWVEEKSYFFRLSA
HHHHCCCEEEECCCCEEEECCCEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCEEEEEHH
YQDKLLALYENQPDFIGPDARRNEITSFVRGGLKDLSISRTTFDWGVRVPHDDEHVMYVW
HHHHHHHEECCCCCCCCCCCHHHHHHHHHHCCHHHCEEEEEEEECCCCCCCCCCCEEEEE
VDALTNYITGVGFPDENDANWKFWPADVHVIGKDIIRFHAVYWPAFLMSAGVPVPKRVYA
HHHHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
HGFLFNRGEKMSKSVGNVVDPFNLADQYGVDQLRYFFLREVPFGQDGNYNHEAIVARINA
HHHEECCCHHHHHHHCCCCCCCCCHHHHCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECC
DLANDLGNLAQRSLSMITKQFGGVLPEPGEFSANDQAILAEADAMIGAARKAMATQQIHQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
WLNAVWSVVAEANRYFAGEAPWALAKTDPKRQHTVLYVTAEVIRQVAILTQPVMPAASAK
HHHHHHHHHHHHHHEECCCCCEEECCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCHHHHH
LLDSLGIPEDERSFARLGGEVRIAPGTQLPAPQAVFPRYIEPSAA
HHHHCCCCCCHHHHHHCCCEEEECCCCCCCCCHHHCCCCCCCCCC
>Mature Secondary Structure 
AKASKTTVKKGKPATKGAATKAAAKKASKSPAKAKAGATKAKAAADKDKKAKAAKANAA
CCCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCHHHCCCCHHHHHCCCHHHHHHHHCCHH
KAKATKGKASSAPAKGKSPKAAKKAAKSATKPAVPAKKTAAKAAATKPAPSKKPAETKPA
HHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCC
PAPKLVAPAVAESPKPPPRAAKPKTPRKRKVEAAAAPEIEIAEIEVAEVEIVEIEATDTA
CCCCHHCCHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEEEEEEEEEEEECCCCC
EDATPVAAATENANTFYVTTAIAYPNGSPHIGHAYEAIATDAIARFARLDGKDVFFLTGT
CCCCCCEEECCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
DEHGLKMVQTAQNEGLTPSELAARNAGRFREMDERLNVSFDRFIRTTEPAHHKSVQAIWT
CCCCHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHH
RMLENGDIYADTYSGWYSVRDEAYYAEEETVVGEDNVRRGPQGTPVEWVEEKSYFFRLSA
HHHHCCCEEEECCCCEEEECCCEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCEEEEEHH
YQDKLLALYENQPDFIGPDARRNEITSFVRGGLKDLSISRTTFDWGVRVPHDDEHVMYVW
HHHHHHHEECCCCCCCCCCCHHHHHHHHHHCCHHHCEEEEEEEECCCCCCCCCCCEEEEE
VDALTNYITGVGFPDENDANWKFWPADVHVIGKDIIRFHAVYWPAFLMSAGVPVPKRVYA
HHHHHHHHHCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
HGFLFNRGEKMSKSVGNVVDPFNLADQYGVDQLRYFFLREVPFGQDGNYNHEAIVARINA
HHHEECCCHHHHHHHCCCCCCCCCHHHHCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECC
DLANDLGNLAQRSLSMITKQFGGVLPEPGEFSANDQAILAEADAMIGAARKAMATQQIHQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
WLNAVWSVVAEANRYFAGEAPWALAKTDPKRQHTVLYVTAEVIRQVAILTQPVMPAASAK
HHHHHHHHHHHHHHEECCCCCEEECCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCHHHHH
LLDSLGIPEDERSFARLGGEVRIAPGTQLPAPQAVFPRYIEPSAA
HHHHCCCCCCHHHHHHCCCEEEECCCCCCCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]