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The map label for this gene is holB [H]

Identifier: 146340770

GI number: 146340770

Start: 3998306

End: 3999346

Strand: Direct

Name: holB [H]

Synonym: BRADO3828

Alternate gene names: 146340770

Gene position: 3998306-3999346 (Clockwise)

Preceding gene: 146340769

Following gene: 146340771

Centisome position: 53.62

GC content: 68.78

Gene sequence:

>1041_bases
ATGAGCGCGCGCAAGGTCGAGCCTGAGATTGCGGTCCGGCATCCGCGCGAGACCACGGCGCTGTTCGGGCATTGCGAGGC
GGAGGCGGCGCTGCTGTCGGCCTACCGCAGCGGACGGATGGCGCATGCCTGGCTGATCGGTGGGCCCCAGGGCATCGGCA
AGGCGACCTTGGCCTATCGCATGGCGCGCTTCGTGCTGACGCACCGCGATCCCAGGGCTGCGGAGGTGCTTGCCGCCGAA
TCGCTCCATGTCGATGCGGACGATCACGTCACCCGGCTGATCGCCTCGGAAGCGCATGGCGGGCTGCTGACCCTGGAGCG
GTCGGCGAACGACAAGGGCGTGCTGCGCACCGTCATCACGGTCGACGAGACGCGCGAGACGATCTCGTTCTTCGGCTCGA
CGGCAGCGGTCGAGGGCTGGCGCATCTGCATCGTCGACACCGTCGACGAGCTCAATCCCAACGCAGCGAACGCGCTATTG
AAGATCCTCGAAGAGCCGCCGCAGCAATCGCTGTTCCTGCTCGTCAGCCACGCGCCGGCGCGGGTGCTCGCCACCATCAA
ATCGCGCTGCCGCCGGCTGTCGTTGCGAGCCTTGTCGACCGAGGACGTCATCAGCGCCGCGGCCGAGGCCATCGGCATGG
ATGGCGGCGAGCCGGCGCTGCGCGAGGCCGCCAAGGCCTCCGAGGGCAGCGTCTCCAGGACCCTGATGCTGATGGGCGGC
GACGGGCTGCAACTGCAGACCCGCACGGCAGCGCTGCTCGCGAGCCTGCCGCGAATCGACGCCGGCGAGCTGCATGCCCT
GGGCGACGCGCTCGGCACCAGCGACCGGGTCGCGCTGGCCAGCTTCCTCGACAGCATCGAACGCTGGGTGGCGGAACGGC
TGCGGGCAGGGGATCCCAACGCGGAACTGCCGCGCCTTGCACGGCTGGCGGAGGTATGGGAAAAGATCGTCCGCGCCGCG
CGCGACACCGAATCCTATAATCTGGAGCGAAAACCGCTGGTTTTCTCGGTGTTCGGGATGCTCGCCGAAGCCACGCGGTA
A

Upstream 100 bases:

>100_bases
GCTGCGTGCTGATCGACGCGACCGCGAGCGCCGAGGCTGTGTCGAATATCGTCTGGGACGCGGTGCGCGAGCGATTGTTC
ACGGCCGTGGCGGCGGCGTC

Downstream 100 bases:

>100_bases
CGCGCCGACCATCATCCCGGGATCATCAGGATCAATCCGTCCACCGACGGCGTCAAAGAGGAATTTGCCGTGGCCAAGGC
CAGCAAGACAACCGTGAAGA

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MSARKVEPEIAVRHPRETTALFGHCEAEAALLSAYRSGRMAHAWLIGGPQGIGKATLAYRMARFVLTHRDPRAAEVLAAE
SLHVDADDHVTRLIASEAHGGLLTLERSANDKGVLRTVITVDETRETISFFGSTAAVEGWRICIVDTVDELNPNAANALL
KILEEPPQQSLFLLVSHAPARVLATIKSRCRRLSLRALSTEDVISAAAEAIGMDGGEPALREAAKASEGSVSRTLMLMGG
DGLQLQTRTAALLASLPRIDAGELHALGDALGTSDRVALASFLDSIERWVAERLRAGDPNAELPRLARLAEVWEKIVRAA
RDTESYNLERKPLVFSVFGMLAEATR

Sequences:

>Translated_346_residues
MSARKVEPEIAVRHPRETTALFGHCEAEAALLSAYRSGRMAHAWLIGGPQGIGKATLAYRMARFVLTHRDPRAAEVLAAE
SLHVDADDHVTRLIASEAHGGLLTLERSANDKGVLRTVITVDETRETISFFGSTAAVEGWRICIVDTVDELNPNAANALL
KILEEPPQQSLFLLVSHAPARVLATIKSRCRRLSLRALSTEDVISAAAEAIGMDGGEPALREAAKASEGSVSRTLMLMGG
DGLQLQTRTAALLASLPRIDAGELHALGDALGTSDRVALASFLDSIERWVAERLRAGDPNAELPRLARLAEVWEKIVRAA
RDTESYNLERKPLVFSVFGMLAEATR
>Mature_345_residues
SARKVEPEIAVRHPRETTALFGHCEAEAALLSAYRSGRMAHAWLIGGPQGIGKATLAYRMARFVLTHRDPRAAEVLAAES
LHVDADDHVTRLIASEAHGGLLTLERSANDKGVLRTVITVDETRETISFFGSTAAVEGWRICIVDTVDELNPNAANALLK
ILEEPPQQSLFLLVSHAPARVLATIKSRCRRLSLRALSTEDVISAAAEAIGMDGGEPALREAAKASEGSVSRTLMLMGGD
GLQLQTRTAALLASLPRIDAGELHALGDALGTSDRVALASFLDSIERWVAERLRAGDPNAELPRLARLAEVWEKIVRAAR
DTESYNLERKPLVFSVFGMLAEATR

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=265, Percent_Identity=27.1698113207547, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787341, Length=257, Percent_Identity=30.3501945525292, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37393; Mature: 37262

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARKVEPEIAVRHPRETTALFGHCEAEAALLSAYRSGRMAHAWLIGGPQGIGKATLAYR
CCCCCCCCCCEECCCCHHHHHHHCCHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHH
MARFVLTHRDPRAAEVLAAESLHVDADDHVTRLIASEAHGGLLTLERSANDKGVLRTVIT
HHHHHHHCCCCHHHHHHHHHHEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEE
VDETRETISFFGSTAAVEGWRICIVDTVDELNPNAANALLKILEEPPQQSLFLLVSHAPA
HHHHHHHHHHHCCHHHCCCEEEEEEECHHHCCCHHHHHHHHHHHCCCCHHEEEEECCCHH
RVLATIKSRCRRLSLRALSTEDVISAAAEAIGMDGGEPALREAAKASEGSVSRTLMLMGG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEEEEEECC
DGLQLQTRTAALLASLPRIDAGELHALGDALGTSDRVALASFLDSIERWVAERLRAGDPN
CCCEEHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
AELPRLARLAEVWEKIVRAARDTESYNLERKPLVFSVFGMLAEATR
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SARKVEPEIAVRHPRETTALFGHCEAEAALLSAYRSGRMAHAWLIGGPQGIGKATLAYR
CCCCCCCCCEECCCCHHHHHHHCCHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHH
MARFVLTHRDPRAAEVLAAESLHVDADDHVTRLIASEAHGGLLTLERSANDKGVLRTVIT
HHHHHHHCCCCHHHHHHHHHHEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEE
VDETRETISFFGSTAAVEGWRICIVDTVDELNPNAANALLKILEEPPQQSLFLLVSHAPA
HHHHHHHHHHHCCHHHCCCEEEEEEECHHHCCCHHHHHHHHHHHCCCCHHEEEEECCCHH
RVLATIKSRCRRLSLRALSTEDVISAAAEAIGMDGGEPALREAAKASEGSVSRTLMLMGG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEEEEEECC
DGLQLQTRTAALLASLPRIDAGELHALGDALGTSDRVALASFLDSIERWVAERLRAGDPN
CCCEEHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
AELPRLARLAEVWEKIVRAARDTESYNLERKPLVFSVFGMLAEATR
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]