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The map label for this gene is nirA [H]

Identifier: 146340739

GI number: 146340739

Start: 3964259

End: 3966076

Strand: Direct

Name: nirA [H]

Synonym: BRADO3793

Alternate gene names: 146340739

Gene position: 3964259-3966076 (Clockwise)

Preceding gene: 146340738

Following gene: 146340740

Centisome position: 53.16

GC content: 65.24

Gene sequence:

>1818_bases
ATGGCCGCTGGAGCTTCGTCCGCCATCATGAAAATCGAGCCGTTGTCTGTCGACTTCAACGACGAGCAGAAGCGCTATCT
GGAAGGCTTGGCCGCCGGCCTGAAGGTCACGCTCGCCGGACGTGGCCAGTCTGCTGCTGCTGTCGCAACCGAGCCGACCG
GGCCGGACGCCCTTCATATCAAAGCGCAGGACAAGCTGCTCGCGGCAGGCCGCAAGCTCGCCGACCAGGAAAAAATCAAG
CGCGAGCTGCATCCGTTCGACGCCTATGAGCGGCTGAAGGCGCAGGCCCGCAGCAATGCCGCACCTTCGGCCGCCGACAA
TTTCCGCTGGCGCTATCACGGCCTGTTCCACGTCGCGCCGGCGCAGAACTCCTACATGTGCCGGCTGCGCATTCCCAACG
GCATCATGAAGCATTGGCAGCTGTCCGGCCTCGCCGATCTCGCCGATCGGCTCTGCGGTCCTTACAGCCACGTCACGACG
CGCGCCAATCTGCAGCTGCGCGAAATCCCGCCCAAGCATGCTGTGATGCTGCTTGAAGGCCTCTCCGATCTCGGCCTCAC
CGCGCGCGGCTCGGGCGCCGACAACATCCGCAACGTCACGGGCACTCCGACCGCCGGCATCGATCCGCAGGAGCTCTTGG
ATACACGGCTCTATGCGCGCGAGTGGCACTTCCACATCCTCAACGACCGCTCGCTCTACAGCCTGCCGCGCAAGTTCAAC
GTCGCCTTCGACGGCGCCGGCCGCATCGCTGCGCTCGAGGACACCAACGACATCGCGTTCTCCGCGGTCGAGGTGAAGGA
CGGCTTTGGCGTTGCGCCGGGCGTTTGGTTCAGGCTCGGCCTCGGTGGCATCACCGGCCATAAGGACTTTGCCAAACCGA
CTGGCATCATTGTCGAGCCGGACAAGGCGACCGAGGTCGCTGATGCCATCGTGCGGCTGTTCATCGAGACCGGCGACCGC
ACCAATCGGCTGAAGGCCCGGCTGAAATACGTTCTCGACAGCATGGGCCATGACAAGTTCATGGAGGCCGTCGAGGCGAG
GCTCGGCCGCACGCTCGCGCGTGTGCCGGACGAGGCGCTGAAGCCAAGGCCCGAGGCGGACCGCATGGCGCATATCGGCG
TGCACCCTCAGAAACAGTCTGGGCTCAACTGGATCGGCGTCGTGCTGCCGCTGGGCAAGCTGACGAGCGATCAGATGCGC
GCGCTCGCAAAGATCGCGGCCGATCTCGGTGACGGCGACATCCGGCTGACGGTCTGGCAGAACCTGCTGCTGTCCGGTGT
GAGCGACGACAAAGTCAGCTTGGCCAGTGCGGCGATCGCGCAGATCGGGCTCGCCACGACAGCCTCGCACATCCGCGCCG
GGCTGATCGCCTGCACGGGCAATGCCGGCTGCAAATTCGCCGCGGCCAACACCAAGAAGCACGCGGCCGAGATCGCCGAC
TGGTGCGAGGGCAGGGTGGGGATGGACACGCCGCTGAACATCCATCTCACCGGCTGCCATCACTCCTGCGCCCAGCACTA
CATCAGCGACATCGGCATGATCGCGGCCAAGGTGCCGGTCGGCGACGGCGATGACACGGTCGAGGGCTATCATCTGTTCG
CCGGTGGCGGCTTCGGTCCGCAGGCCGATATCGGCCGCGAGGTGTATCGCGACCTCAAAGCTGAGGACGCGCCGGGTACC
GTCGAGAAGCTGCTCAAGGCCTATCTCGCCCATCGCACTTCGCCGGAAGAGACCTTCTTGTCGTTTGCCCGCCGCCATGA
CGGCGACGCTCTTCGCAAGCTCGCCGAAGCCCAGACCTCGTCCGAGGTGTCAGCATGA

Upstream 100 bases:

>100_bases
TTCCGCAGCAACGAAGCTGGTCGGATCGCTCTCCTGAAGAAGGCCAAGGCTGGCCGTTCGGGCGCCGCGATCCTCATCCC
ATCCCCCGACGCCAATCGCC

Downstream 100 bases:

>100_bases
ACCAGATCACACCGATGCCGAAGCTCGAGATCATTCCTCCGACCGCGCCGTTCTCGGAAGCGCAGCGCTCCTGGCTCAAC
GGCTTCCTCGCCGGCGCGCT

Product: ferredoxin-nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 605; Mature: 604

Protein sequence:

>605_residues
MAAGASSAIMKIEPLSVDFNDEQKRYLEGLAAGLKVTLAGRGQSAAAVATEPTGPDALHIKAQDKLLAAGRKLADQEKIK
RELHPFDAYERLKAQARSNAAPSAADNFRWRYHGLFHVAPAQNSYMCRLRIPNGIMKHWQLSGLADLADRLCGPYSHVTT
RANLQLREIPPKHAVMLLEGLSDLGLTARGSGADNIRNVTGTPTAGIDPQELLDTRLYAREWHFHILNDRSLYSLPRKFN
VAFDGAGRIAALEDTNDIAFSAVEVKDGFGVAPGVWFRLGLGGITGHKDFAKPTGIIVEPDKATEVADAIVRLFIETGDR
TNRLKARLKYVLDSMGHDKFMEAVEARLGRTLARVPDEALKPRPEADRMAHIGVHPQKQSGLNWIGVVLPLGKLTSDQMR
ALAKIAADLGDGDIRLTVWQNLLLSGVSDDKVSLASAAIAQIGLATTASHIRAGLIACTGNAGCKFAAANTKKHAAEIAD
WCEGRVGMDTPLNIHLTGCHHSCAQHYISDIGMIAAKVPVGDGDDTVEGYHLFAGGGFGPQADIGREVYRDLKAEDAPGT
VEKLLKAYLAHRTSPEETFLSFARRHDGDALRKLAEAQTSSEVSA

Sequences:

>Translated_605_residues
MAAGASSAIMKIEPLSVDFNDEQKRYLEGLAAGLKVTLAGRGQSAAAVATEPTGPDALHIKAQDKLLAAGRKLADQEKIK
RELHPFDAYERLKAQARSNAAPSAADNFRWRYHGLFHVAPAQNSYMCRLRIPNGIMKHWQLSGLADLADRLCGPYSHVTT
RANLQLREIPPKHAVMLLEGLSDLGLTARGSGADNIRNVTGTPTAGIDPQELLDTRLYAREWHFHILNDRSLYSLPRKFN
VAFDGAGRIAALEDTNDIAFSAVEVKDGFGVAPGVWFRLGLGGITGHKDFAKPTGIIVEPDKATEVADAIVRLFIETGDR
TNRLKARLKYVLDSMGHDKFMEAVEARLGRTLARVPDEALKPRPEADRMAHIGVHPQKQSGLNWIGVVLPLGKLTSDQMR
ALAKIAADLGDGDIRLTVWQNLLLSGVSDDKVSLASAAIAQIGLATTASHIRAGLIACTGNAGCKFAAANTKKHAAEIAD
WCEGRVGMDTPLNIHLTGCHHSCAQHYISDIGMIAAKVPVGDGDDTVEGYHLFAGGGFGPQADIGREVYRDLKAEDAPGT
VEKLLKAYLAHRTSPEETFLSFARRHDGDALRKLAEAQTSSEVSA
>Mature_604_residues
AAGASSAIMKIEPLSVDFNDEQKRYLEGLAAGLKVTLAGRGQSAAAVATEPTGPDALHIKAQDKLLAAGRKLADQEKIKR
ELHPFDAYERLKAQARSNAAPSAADNFRWRYHGLFHVAPAQNSYMCRLRIPNGIMKHWQLSGLADLADRLCGPYSHVTTR
ANLQLREIPPKHAVMLLEGLSDLGLTARGSGADNIRNVTGTPTAGIDPQELLDTRLYAREWHFHILNDRSLYSLPRKFNV
AFDGAGRIAALEDTNDIAFSAVEVKDGFGVAPGVWFRLGLGGITGHKDFAKPTGIIVEPDKATEVADAIVRLFIETGDRT
NRLKARLKYVLDSMGHDKFMEAVEARLGRTLARVPDEALKPRPEADRMAHIGVHPQKQSGLNWIGVVLPLGKLTSDQMRA
LAKIAADLGDGDIRLTVWQNLLLSGVSDDKVSLASAAIAQIGLATTASHIRAGLIACTGNAGCKFAAANTKKHAAEIADW
CEGRVGMDTPLNIHLTGCHHSCAQHYISDIGMIAAKVPVGDGDDTVEGYHLFAGGGFGPQADIGREVYRDLKAEDAPGTV
EKLLKAYLAHRTSPEETFLSFARRHDGDALRKLAEAQTSSEVSA

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=467, Percent_Identity=23.1263383297645, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6322597, Length=523, Percent_Identity=22.7533460803059, Blast_Score=80, Evalue=7e-16,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.7.7.1 [H]

Molecular weight: Translated: 65303; Mature: 65172

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAGASSAIMKIEPLSVDFNDEQKRYLEGLAAGLKVTLAGRGQSAAAVATEPTGPDALHI
CCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCEEEEEECCCCCCEEEEECCCCCCEEEE
KAQDKLLAAGRKLADQEKIKRELHPFDAYERLKAQARSNAAPSAADNFRWRYHGLFHVAP
ECHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEC
AQNSYMCRLRIPNGIMKHWQLSGLADLADRLCGPYSHVTTRANLQLREIPPKHAVMLLEG
CCCCEEEEEECCCHHHHHHHHCCHHHHHHHHCCCHHHHEEECCEEEECCCCHHHHHHHHC
LSDLGLTARGSGADNIRNVTGTPTAGIDPQELLDTRLYAREWHFHILNDRSLYSLPRKFN
HHHCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCHHHHCCCCEE
VAFDGAGRIAALEDTNDIAFSAVEVKDGFGVAPGVWFRLGLGGITGHKDFAKPTGIIVEP
EEECCCCCEEEEECCCCEEEEEEEECCCCCCCCCHHHEECCCCCCCCCCCCCCCCEEECC
DKATEVADAIVRLFIETGDRTNRLKARLKYVLDSMGHDKFMEAVEARLGRTLARVPDEAL
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHH
KPRPEADRMAHIGVHPQKQSGLNWIGVVLPLGKLTSDQMRALAKIAADLGDGDIRLTVWQ
CCCCCHHCEEECCCCCCHHCCCCEEEEEHCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHH
NLLLSGVSDDKVSLASAAIAQIGLATTASHIRAGLIACTGNAGCKFAAANTKKHAAEIAD
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEECCCHHHHHHHHH
WCEGRVGMDTPLNIHLTGCHHSCAQHYISDIGMIAAKVPVGDGDDTVEGYHLFAGGGFGP
HHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHEEECCCCCCCCCCCCEEEEECCCCCC
QADIGREVYRDLKAEDAPGTVEKLLKAYLAHRTSPEETFLSFARRHDGDALRKLAEAQTS
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
SEVSA
CCCCC
>Mature Secondary Structure 
AAGASSAIMKIEPLSVDFNDEQKRYLEGLAAGLKVTLAGRGQSAAAVATEPTGPDALHI
CCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCEEEEEECCCCCCEEEEECCCCCCEEEE
KAQDKLLAAGRKLADQEKIKRELHPFDAYERLKAQARSNAAPSAADNFRWRYHGLFHVAP
ECHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEC
AQNSYMCRLRIPNGIMKHWQLSGLADLADRLCGPYSHVTTRANLQLREIPPKHAVMLLEG
CCCCEEEEEECCCHHHHHHHHCCHHHHHHHHCCCHHHHEEECCEEEECCCCHHHHHHHHC
LSDLGLTARGSGADNIRNVTGTPTAGIDPQELLDTRLYAREWHFHILNDRSLYSLPRKFN
HHHCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCHHHHCCCCEE
VAFDGAGRIAALEDTNDIAFSAVEVKDGFGVAPGVWFRLGLGGITGHKDFAKPTGIIVEP
EEECCCCCEEEEECCCCEEEEEEEECCCCCCCCCHHHEECCCCCCCCCCCCCCCCEEECC
DKATEVADAIVRLFIETGDRTNRLKARLKYVLDSMGHDKFMEAVEARLGRTLARVPDEAL
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHH
KPRPEADRMAHIGVHPQKQSGLNWIGVVLPLGKLTSDQMRALAKIAADLGDGDIRLTVWQ
CCCCCHHCEEECCCCCCHHCCCCEEEEEHCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHH
NLLLSGVSDDKVSLASAAIAQIGLATTASHIRAGLIACTGNAGCKFAAANTKKHAAEIAD
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEECCCHHHHHHHHH
WCEGRVGMDTPLNIHLTGCHHSCAQHYISDIGMIAAKVPVGDGDDTVEGYHLFAGGGFGP
HHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHEEECCCCCCCCCCCCEEEEECCCCCC
QADIGREVYRDLKAEDAPGTVEKLLKAYLAHRTSPEETFLSFARRHDGDALRKLAEAQTS
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
SEVSA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8490140 [H]