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The map label for this gene is xthA [H]

Identifier: 146340267

GI number: 146340267

Start: 3486489

End: 3487277

Strand: Reverse

Name: xthA [H]

Synonym: BRADO3288

Alternate gene names: 146340267

Gene position: 3487277-3486489 (Counterclockwise)

Preceding gene: 146340269

Following gene: 146340264

Centisome position: 46.77

GC content: 64.77

Gene sequence:

>789_bases
ATGAAGATTGCGACATTCAACATCAACAATGTCAGGCGACGGCTCCCCAACTTGCTGGCCTGGCTGCGCGCCGCCAAGCC
GGACATCGTCTGCCTGCAGGAGCTGAAATGTACGCTGGCCGAATTTCCGGTCGAGGAGATCGCCAAAGCCGGCTACGGCG
CTGTCTGGCAGGGCCAGAAGACCTGGAACGGGGTCGCGATCCTCGCCCGCAAAGCCGAGCCGGTGCTGATCCGCACCGCC
CTGCCCGGCGATCCCAGCGACAAGGAGGCGCGTTACATCGAGGCGGCGGTGCGCGGCATCGTCATCGCCTGCCTGTATCT
GCCGAATGGCAATCCGCAGCCGGGTCCGAAATTCGACTATAAGCTCGCCTGGTTCAAGCGGCTGCACAAGCACGCGGCCA
AGTTCATCAAGGACGACCTGCCCGTCGTGCTCGCCGGCGACTACAATGTCGCGCCGACGCCGCTCGACATCTATCCGACG
AAATCGTGGGACAAGGATGCTTTGATCCAGCCCAAGAGCCGCGCAGCCTATGCGGCGCTGGTCAATCAGGGCTGGTGCGA
CGCGATCCGCACGCTGCACCCGGACGCGCCGATGTTCACGTTCTGGGACTACAAGCGCAACCGCTGGCCGCGCGATGCGG
GACTGCGGCTCGATCATCTGCTGCTCAGCCCGCAACTCGCGCCACGGCTCAAAACCGCCGGCGTCGACCGCGCCATTCGC
GGCGAGGACGGCGCCAGCGACCACGCACCGGCCTGGATCATGTTGAAATCGACCCGCGCCTCGCCATAA

Upstream 100 bases:

>100_bases
GCAGCTGCTGTGCATGCGCAGTCGCGACGCCCGACGCCAGCGTGAACAAAATGGCGGCTGCGAACTTTGGCATCAGGTCT
TTCCTCGCTCCGGCGGCATC

Downstream 100 bases:

>100_bases
CACGCTCTCCGCATGTTGGCGGAGAGAGTGTGCAGCGCCGGCCTCGGCCCTGCCCCTCTCCATGACTGTCGTATTCATGC
TCCAGCGTGTAGCGCCGACC

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MKIATFNINNVRRRLPNLLAWLRAAKPDIVCLQELKCTLAEFPVEEIAKAGYGAVWQGQKTWNGVAILARKAEPVLIRTA
LPGDPSDKEARYIEAAVRGIVIACLYLPNGNPQPGPKFDYKLAWFKRLHKHAAKFIKDDLPVVLAGDYNVAPTPLDIYPT
KSWDKDALIQPKSRAAYAALVNQGWCDAIRTLHPDAPMFTFWDYKRNRWPRDAGLRLDHLLLSPQLAPRLKTAGVDRAIR
GEDGASDHAPAWIMLKSTRASP

Sequences:

>Translated_262_residues
MKIATFNINNVRRRLPNLLAWLRAAKPDIVCLQELKCTLAEFPVEEIAKAGYGAVWQGQKTWNGVAILARKAEPVLIRTA
LPGDPSDKEARYIEAAVRGIVIACLYLPNGNPQPGPKFDYKLAWFKRLHKHAAKFIKDDLPVVLAGDYNVAPTPLDIYPT
KSWDKDALIQPKSRAAYAALVNQGWCDAIRTLHPDAPMFTFWDYKRNRWPRDAGLRLDHLLLSPQLAPRLKTAGVDRAIR
GEDGASDHAPAWIMLKSTRASP
>Mature_262_residues
MKIATFNINNVRRRLPNLLAWLRAAKPDIVCLQELKCTLAEFPVEEIAKAGYGAVWQGQKTWNGVAILARKAEPVLIRTA
LPGDPSDKEARYIEAAVRGIVIACLYLPNGNPQPGPKFDYKLAWFKRLHKHAAKFIKDDLPVVLAGDYNVAPTPLDIYPT
KSWDKDALIQPKSRAAYAALVNQGWCDAIRTLHPDAPMFTFWDYKRNRWPRDAGLRLDHLLLSPQLAPRLKTAGVDRAIR
GEDGASDHAPAWIMLKSTRASP

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=262, Percent_Identity=30.5343511450382, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI18375503, Length=262, Percent_Identity=30.5343511450382, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI18375501, Length=262, Percent_Identity=30.5343511450382, Blast_Score=115, Evalue=3e-26,
Organism=Escherichia coli, GI1788046, Length=268, Percent_Identity=33.955223880597, Blast_Score=145, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71989536, Length=239, Percent_Identity=27.6150627615063, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI221330655, Length=269, Percent_Identity=30.4832713754647, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI17136678, Length=269, Percent_Identity=30.4832713754647, Blast_Score=101, Evalue=5e-22,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29336; Mature: 29336

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIATFNINNVRRRLPNLLAWLRAAKPDIVCLQELKCTLAEFPVEEIAKAGYGAVWQGQK
CEEEEECHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCHHHHHHCCCCCEECCCC
TWNGVAILARKAEPVLIRTALPGDPSDKEARYIEAAVRGIVIACLYLPNGNPQPGPKFDY
CCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCH
KLAWFKRLHKHAAKFIKDDLPVVLAGDYNVAPTPLDIYPTKSWDKDALIQPKSRAAYAAL
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHH
VNQGWCDAIRTLHPDAPMFTFWDYKRNRWPRDAGLRLDHLLLSPQLAPRLKTAGVDRAIR
HCCCHHHHHHHCCCCCCEEEEECCHHCCCCCCCCCCHHHHHHCCCCCCHHHHCCCCCCCC
GEDGASDHAPAWIMLKSTRASP
CCCCCCCCCCEEEEEECCCCCC
>Mature Secondary Structure
MKIATFNINNVRRRLPNLLAWLRAAKPDIVCLQELKCTLAEFPVEEIAKAGYGAVWQGQK
CEEEEECHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCHHHHHHCCCCCEECCCC
TWNGVAILARKAEPVLIRTALPGDPSDKEARYIEAAVRGIVIACLYLPNGNPQPGPKFDY
CCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCH
KLAWFKRLHKHAAKFIKDDLPVVLAGDYNVAPTPLDIYPTKSWDKDALIQPKSRAAYAAL
HHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHH
VNQGWCDAIRTLHPDAPMFTFWDYKRNRWPRDAGLRLDHLLLSPQLAPRLKTAGVDRAIR
HCCCHHHHHHHCCCCCCEEEEECCHHCCCCCCCCCCHHHHHHCCCCCCHHHHCCCCCCCC
GEDGASDHAPAWIMLKSTRASP
CCCCCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]