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The map label for this gene is yfkN [H]

Identifier: 146340167

GI number: 146340167

Start: 3384908

End: 3386554

Strand: Direct

Name: yfkN [H]

Synonym: BRADO3183

Alternate gene names: 146340167

Gene position: 3384908-3386554 (Clockwise)

Preceding gene: 146340166

Following gene: 146340168

Centisome position: 45.39

GC content: 65.51

Gene sequence:

>1647_bases
TTGGCGGCGGCTGCCTTGTCGGGCGGCACGTCCCGCGCCTTTGCGCAGCAGAAGCTGACCGAGAAGGAGCTTCTTGCGTT
CGATCCGGTCGGCAATGTCACGCTGGTGCATGTCACCGACATCCACGGCCAGCTCATGCCGCTGTATTTCCGCGAGCCGT
CGACCAATCTCGGCGTCGGCGACGCCAAGGGCCAGCCGCCGCATGTCACCGGCAAGGATTTTCTCACCCGCTTCGGCATC
GCGCCGGGCTCGTCGTCGGCCTATGCGCTGACCTCCGAGGATTTCGAGGCGCTCGCCAAGACCTACGGGCGGATCGGCGG
GCTCGACCGCGCCGCCACCGTCATCAAGGCCATCCGCGCCGAGCGCGGCGACAAGGTCGTGCTGCTCGATGGCGGCGACA
CCTGGCAGGGATCCTGGACGTCATTGAAGACACGTGGCCAGGACATGATCGACTGCATGGTGCTGCTCAAGCCCGATGCG
ATGACGGGGCATTGGGAGTTCACCTACGGCAGCGAGCGCGTCAAGCAGGCAGTCGATGCGCTCGGCTTCCCGTTCCTCGG
TCTCAACATCCGCGACACCGAGTGGAACGAGCCGGCGTTCGAAGCCTCGACGATGATTGAGCGCGGTGGCCTCAAGATCG
CGGTGCTCGGCCAGGCCTTTCCGTACACGCCCGTCGCCAATCCGCGCTGGATGATTCCGAACTGGTCGTTCGGCGTGCGC
GAGGAGGATGTCCAGACCCAGGTCGACAAGGCGCGCAAGGACGGCGCAGGTCTGGTGGTGCTGCTGTCGCACAACGGCTT
CGACGTGGACCGTAAGCTGGCGAGCCGCGTCAAGGGCCTCGACGTCATCCTCACCGGTCACACCCATGATGCGCTGCCCG
AGGCGGTCAAGGTCGGCAAGACGCTGCTGATCGCGTCGGGGTCGTCCGGCAAGTTCGTCTCGCGGCTCGATCTCGACGTC
AGGGATGGCGAGGTCAAGGGCTATCGTTACAAGCTGATCCCGCTGTTCTCCGACGTGATCACGCCTGATCCGGCGATGGC
CGCCAAGATCGCCGAGGTGCGCAAGCCGTTTGCCGCCGATCTCGGCCGTGCGCTGGGACGGACCGAAACGCTGCTATATC
GCCGGGGGAATTTCAACGGCACGTTCGACGACCTGATCTGCCAGGCGCTGCTGCAGGAGCGCGACGCGGAGATCGCGCTG
TCGCCGGGCTTCCGCTGGGGCACCAGCGTGATGCCGGGCCAGGACATCACCTTCGAGGACGTCACCAACGCCACCGCGAT
CACCTATCCCGCGGTGTATCGGATGGGCATGACCGGCACGCGCCTCAAGGAGATCATCGAGGACGTCGCCGACAATCTGT
TCAACGTCGACCCGTACTACCAGCAGGGCGGCGACATGGTTCGCATCGGCGGCATGTCCTATGCGATCGACGTCAACAAG
CCGCAGGGGCAGCGCATCTCCGACATGCGCCTGATCAAGACCGGCGCCGCGATCGATCCGGCGCGCGAATATCAGGTCGC
CGGCTGGGCCAGCGTCAACGAAGGCACGCAGGGCCCGCCGATCTGGGAGGTCGTGTCGAGCTATCTGCAGCGCCAGAAAA
CCGTGCGTCTCGAGCCCAACCGGGCCGTCAAAGTCTCCGGAGTGTGA

Upstream 100 bases:

>100_bases
GGCGCGGCATCGGTCTGCCCGTCGAGACACCGTCGGTCCGCAACTAGCGGACACAAGGATAGACATCATGATATCGCGTC
GCGAATTCGTTCAGGTCGCA

Downstream 100 bases:

>100_bases
CGACGATGTCCAAGGACAAATCGCTTTCCTCAGCCCCACGCGCGTCGCGGCGCGGCTTCCTGACCGCGGCGGGCGGCCTC
GCGGCCACGGCGCTGGCGTC

Product: putative sulfur/thiosulfate oxidation protein (SoxB)

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MAAAALSGGTSRAFAQQKLTEKELLAFDPVGNVTLVHVTDIHGQLMPLYFREPSTNLGVGDAKGQPPHVTGKDFLTRFGI
APGSSSAYALTSEDFEALAKTYGRIGGLDRAATVIKAIRAERGDKVVLLDGGDTWQGSWTSLKTRGQDMIDCMVLLKPDA
MTGHWEFTYGSERVKQAVDALGFPFLGLNIRDTEWNEPAFEASTMIERGGLKIAVLGQAFPYTPVANPRWMIPNWSFGVR
EEDVQTQVDKARKDGAGLVVLLSHNGFDVDRKLASRVKGLDVILTGHTHDALPEAVKVGKTLLIASGSSGKFVSRLDLDV
RDGEVKGYRYKLIPLFSDVITPDPAMAAKIAEVRKPFAADLGRALGRTETLLYRRGNFNGTFDDLICQALLQERDAEIAL
SPGFRWGTSVMPGQDITFEDVTNATAITYPAVYRMGMTGTRLKEIIEDVADNLFNVDPYYQQGGDMVRIGGMSYAIDVNK
PQGQRISDMRLIKTGAAIDPAREYQVAGWASVNEGTQGPPIWEVVSSYLQRQKTVRLEPNRAVKVSGV

Sequences:

>Translated_548_residues
MAAAALSGGTSRAFAQQKLTEKELLAFDPVGNVTLVHVTDIHGQLMPLYFREPSTNLGVGDAKGQPPHVTGKDFLTRFGI
APGSSSAYALTSEDFEALAKTYGRIGGLDRAATVIKAIRAERGDKVVLLDGGDTWQGSWTSLKTRGQDMIDCMVLLKPDA
MTGHWEFTYGSERVKQAVDALGFPFLGLNIRDTEWNEPAFEASTMIERGGLKIAVLGQAFPYTPVANPRWMIPNWSFGVR
EEDVQTQVDKARKDGAGLVVLLSHNGFDVDRKLASRVKGLDVILTGHTHDALPEAVKVGKTLLIASGSSGKFVSRLDLDV
RDGEVKGYRYKLIPLFSDVITPDPAMAAKIAEVRKPFAADLGRALGRTETLLYRRGNFNGTFDDLICQALLQERDAEIAL
SPGFRWGTSVMPGQDITFEDVTNATAITYPAVYRMGMTGTRLKEIIEDVADNLFNVDPYYQQGGDMVRIGGMSYAIDVNK
PQGQRISDMRLIKTGAAIDPAREYQVAGWASVNEGTQGPPIWEVVSSYLQRQKTVRLEPNRAVKVSGV
>Mature_547_residues
AAAALSGGTSRAFAQQKLTEKELLAFDPVGNVTLVHVTDIHGQLMPLYFREPSTNLGVGDAKGQPPHVTGKDFLTRFGIA
PGSSSAYALTSEDFEALAKTYGRIGGLDRAATVIKAIRAERGDKVVLLDGGDTWQGSWTSLKTRGQDMIDCMVLLKPDAM
TGHWEFTYGSERVKQAVDALGFPFLGLNIRDTEWNEPAFEASTMIERGGLKIAVLGQAFPYTPVANPRWMIPNWSFGVRE
EDVQTQVDKARKDGAGLVVLLSHNGFDVDRKLASRVKGLDVILTGHTHDALPEAVKVGKTLLIASGSSGKFVSRLDLDVR
DGEVKGYRYKLIPLFSDVITPDPAMAAKIAEVRKPFAADLGRALGRTETLLYRRGNFNGTFDDLICQALLQERDAEIALS
PGFRWGTSVMPGQDITFEDVTNATAITYPAVYRMGMTGTRLKEIIEDVADNLFNVDPYYQQGGDMVRIGGMSYAIDVNKP
QGQRISDMRLIKTGAAIDPAREYQVAGWASVNEGTQGPPIWEVVSSYLQRQKTVRLEPNRAVKVSGV

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=467, Percent_Identity=22.9122055674518, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1790658, Length=231, Percent_Identity=27.7056277056277, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28573524, Length=453, Percent_Identity=22.5165562913907, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19922444, Length=224, Percent_Identity=29.4642857142857, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922446, Length=435, Percent_Identity=22.9885057471264, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24654424, Length=435, Percent_Identity=22.9885057471264, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI221329836, Length=469, Percent_Identity=24.7334754797441, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24641187, Length=198, Percent_Identity=31.8181818181818, Blast_Score=69, Evalue=9e-12,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 59836; Mature: 59705

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAAALSGGTSRAFAQQKLTEKELLAFDPVGNVTLVHVTDIHGQLMPLYFREPSTNLGVG
CCCCCCCCCCHHHHHHHHCCHHHHEEECCCCCEEEEEEECCCCCEEEEEEECCCCCCCCC
DAKGQPPHVTGKDFLTRFGIAPGSSSAYALTSEDFEALAKTYGRIGGLDRAATVIKAIRA
CCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ERGDKVVLLDGGDTWQGSWTSLKTRGQDMIDCMVLLKPDAMTGHWEFTYGSERVKQAVDA
CCCCEEEEECCCCCCCCCCHHHHHCCCCCEEEEEEECCCCCCCEEEEECCHHHHHHHHHH
LGFPFLGLNIRDTEWNEPAFEASTMIERGGLKIAVLGQAFPYTPVANPRWMIPNWSFGVR
CCCCEECCEEECCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCEECCCCCCCCC
EEDVQTQVDKARKDGAGLVVLLSHNGFDVDRKLASRVKGLDVILTGHTHDALPEAVKVGK
CHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCC
TLLIASGSSGKFVSRLDLDVRDGEVKGYRYKLIPLFSDVITPDPAMAAKIAEVRKPFAAD
EEEEEECCCCCEEEEEECEEECCCCCCEEEEEEEHHHHHCCCCHHHHHHHHHHHCHHHHH
LGRALGRTETLLYRRGNFNGTFDDLICQALLQERDAEIALSPGFRWGTSVMPGQDITFED
HHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCHHH
VTNATAITYPAVYRMGMTGTRLKEIIEDVADNLFNVDPYYQQGGDMVRIGGMSYAIDVNK
CCCCEEEEEHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEECCEEEEEECCC
PQGQRISDMRLIKTGAAIDPAREYQVAGWASVNEGTQGPPIWEVVSSYLQRQKTVRLEPN
CCCCCCCCEEEEECCCCCCCHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHCCEEEECCC
RAVKVSGV
CEEEECCC
>Mature Secondary Structure 
AAAALSGGTSRAFAQQKLTEKELLAFDPVGNVTLVHVTDIHGQLMPLYFREPSTNLGVG
CCCCCCCCCHHHHHHHHCCHHHHEEECCCCCEEEEEEECCCCCEEEEEEECCCCCCCCC
DAKGQPPHVTGKDFLTRFGIAPGSSSAYALTSEDFEALAKTYGRIGGLDRAATVIKAIRA
CCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ERGDKVVLLDGGDTWQGSWTSLKTRGQDMIDCMVLLKPDAMTGHWEFTYGSERVKQAVDA
CCCCEEEEECCCCCCCCCCHHHHHCCCCCEEEEEEECCCCCCCEEEEECCHHHHHHHHHH
LGFPFLGLNIRDTEWNEPAFEASTMIERGGLKIAVLGQAFPYTPVANPRWMIPNWSFGVR
CCCCEECCEEECCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCEECCCCCCCCC
EEDVQTQVDKARKDGAGLVVLLSHNGFDVDRKLASRVKGLDVILTGHTHDALPEAVKVGK
CHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCC
TLLIASGSSGKFVSRLDLDVRDGEVKGYRYKLIPLFSDVITPDPAMAAKIAEVRKPFAAD
EEEEEECCCCCEEEEEECEEECCCCCCEEEEEEEHHHHHCCCCHHHHHHHHHHHCHHHHH
LGRALGRTETLLYRRGNFNGTFDDLICQALLQERDAEIALSPGFRWGTSVMPGQDITFED
HHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCHHH
VTNATAITYPAVYRMGMTGTRLKEIIEDVADNLFNVDPYYQQGGDMVRIGGMSYAIDVNK
CCCCEEEEEHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEECCEEEEEECCC
PQGQRISDMRLIKTGAAIDPAREYQVAGWASVNEGTQGPPIWEVVSSYLQRQKTVRLEPN
CCCCCCCCEEEEECCCCCCCHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHCCEEEECCC
RAVKVSGV
CEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]