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The map label for this gene is fusA [H]

Identifier: 146340054

GI number: 146340054

Start: 3269595

End: 3271667

Strand: Direct

Name: fusA [H]

Synonym: BRADO3063

Alternate gene names: 146340054

Gene position: 3269595-3271667 (Clockwise)

Preceding gene: 161353767

Following gene: 146340055

Centisome position: 43.85

GC content: 63.29

Gene sequence:

>2073_bases
ATGCCCCGCCAACACGCCATCGAAGACTATCGTAACTTCGGTATCATGGCGCATATCGACGCCGGTAAGACGACGACGAC
CGAGCGCATCCTGTATTACACCGGCAAGAGCCACAAGATCGGCGAAGTGCACGAAGGTGCCGCGACGATGGACTGGATGG
AGCAGGAGCAGGAGCGTGGCATCACGATCACTTCGGCTGCCACCACCGCGTTCTGGAACGGCAAGCGCCTGAACATCATC
GACACTCCCGGCCACGTCGACTTCACCATTGAGGTCGAGCGTTCGCTCCGCGTGCTCGACGGCGCCGTTTGCGTGCTCGA
TTCCAACCAGGGCGTTGAGCCGCAGACCGAGACCGTCTGGCGCCAGGGCGACAAGTACAAGGTGCCGCGGATCGTCTTCG
CGAACAAGATGGACAAGACCGGCGCCGACTTCTTCAAGTGCCTGTCCGACATCGTCGACCGCCTGGGCGCGAAGCCGGTG
GCCATCCAGCTGCCGATCGGCGCCGAGAACAACTTCAAGGGCTGCATCGATCTCGTGACCATGAAGGCGATCGTCTGGAA
CGACGAGTCGCTCGGCGCTAAGTTCGATCATGTCGAGATCCCGGCTGAGCTGGCCGACCAGGCCAAGGAATATCGCGAGA
AGCTGGTGGAAGCCGCCGTCGAGCTCGACGACGACGCGCTGACCGCGTTCCTGGACGGCAAGGAGCCGGACGAGGCGACG
CTGAAGCAGCTGATCCGCAAGGCCGTGCTGACCGGCGCGTTCTATCCCGTCCTGTGCGGCTCGGCCTTCAAGAACAAGGG
CGTGCAGCCCTTGCTCGACGCTGTCGTCGACTATCTGCCGTCGCCGCTCGACGTGCCCGCGATCAAGGGCACCGACGACC
GCGGCAACGAGGTCGTGCGCAAGGCGGACGACAAGGAGCCGCTGTCGCTGCTGGCGTTCAAGATCATGGACGACCCGTTC
GTCGGCACCATCACCTTCTGCCGCATCTATTCGGGCATCCTGACCTCGGGCACCGGCGTCGTGAACTCGACCCGCGAGAA
GAAGGAGCGCATCGGCCGCATGCTGCTGATGCACGCGAACAACCGCGAGGACATCAAGGAAGCCTATGCGGGCGACATCG
TCGCTCTTGCCGGCCTCAAGGAAGCCCGCACCGGCGACACGCTCTGCGATCCCAACAACCAGGTGATCCTGGAAAAGATG
GAATTCCCGGAGCCGGTCATCGAGATCGCGATCGAGCCGAAGTCGAAGGCCGACCAGGAGAAGCTGGGCATCGCTTTGGC
GAAGCTCGCCGCGGAGGATCCGTCCTTCCGCGTGTCGACCGATCACGAGTCGGGCCAGACCATCCTCAAGGGCATGGGCG
AGCTCCATCTCGACATCAAGGTCGACATCCTGAAGCGCACCTACAAGGTCGATGCGAATATCGGCGCGCCGCAGGTGGCG
TTCCGTGAGCGCATCACCAAGCCGGCCAATGTTGACTACACCCACAAGAAGCAGACCGGCGGCACCGGCCAGTTCGCGGC
CGTCAGCCTCGTGGTCGAGCCGAACGAGCCGGGTGGCGGCTACGTGTTCGAGTCCAAGATCGTCGGCGGTGCGGTTCCGA
AGGAATACATCCCGGGCGTCGAAAAGGGCATCGAGAGCGTGCTCGGCGCGGGCGTGGTCGCGGGCTTCCCGGTGGTCGAC
GTCAAGGTGGCGCTGGTCGACGGCAAGTATCACGACGTCGACTCGTCGGCACTGGCCTTCGAAATCGCCTCCCGCGCGGC
TTTCCGCGAAGCGCTGCAGAAGGGCAAGTCGGTTCTGCTCGAGCCGATCATGAAGGTCGAGGTCGTGACCCCGGAAGACT
ACACCGGTTCGGTGATCGGCGACCTGAACTCGCGTCGTGGCCAGATCCAGGGCCAGGACATGCGCGGCAACGCCAACGTC
ATCAACGCGATGGTGCCGCTCATGAACATGTTCGGTTACGTGAACAATCTGCGCTCGATGAGCCAGGGTCGCGCGACCTT
CACGATGCAGTTCTCGCACTACGCTGAAGCGCCGGCCAACGTGTCGGCTGAAGTCCAGAAGAAGTTTGCCTGA

Upstream 100 bases:

>100_bases
GAAGAAGCGTGAAGACGTGCACCGGATGGCGGAAGCCAACCGCGCCTTCTCGCACTACCGCTGGTAACAGCGGCCCGAAC
GGAAATCAGGGAACAGATCC

Downstream 100 bases:

>100_bases
TTGTCGTTAGCTCAAGTTAGCGACTGAACGGAGAGTCAAATGGCCAAAGCTAAGTTTGAACGTAACAAGCCCCACTGCAA
CATCGGCACCATCGGTCACG

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 690; Mature: 689

Protein sequence:

>690_residues
MPRQHAIEDYRNFGIMAHIDAGKTTTTERILYYTGKSHKIGEVHEGAATMDWMEQEQERGITITSAATTAFWNGKRLNII
DTPGHVDFTIEVERSLRVLDGAVCVLDSNQGVEPQTETVWRQGDKYKVPRIVFANKMDKTGADFFKCLSDIVDRLGAKPV
AIQLPIGAENNFKGCIDLVTMKAIVWNDESLGAKFDHVEIPAELADQAKEYREKLVEAAVELDDDALTAFLDGKEPDEAT
LKQLIRKAVLTGAFYPVLCGSAFKNKGVQPLLDAVVDYLPSPLDVPAIKGTDDRGNEVVRKADDKEPLSLLAFKIMDDPF
VGTITFCRIYSGILTSGTGVVNSTREKKERIGRMLLMHANNREDIKEAYAGDIVALAGLKEARTGDTLCDPNNQVILEKM
EFPEPVIEIAIEPKSKADQEKLGIALAKLAAEDPSFRVSTDHESGQTILKGMGELHLDIKVDILKRTYKVDANIGAPQVA
FRERITKPANVDYTHKKQTGGTGQFAAVSLVVEPNEPGGGYVFESKIVGGAVPKEYIPGVEKGIESVLGAGVVAGFPVVD
VKVALVDGKYHDVDSSALAFEIASRAAFREALQKGKSVLLEPIMKVEVVTPEDYTGSVIGDLNSRRGQIQGQDMRGNANV
INAMVPLMNMFGYVNNLRSMSQGRATFTMQFSHYAEAPANVSAEVQKKFA

Sequences:

>Translated_690_residues
MPRQHAIEDYRNFGIMAHIDAGKTTTTERILYYTGKSHKIGEVHEGAATMDWMEQEQERGITITSAATTAFWNGKRLNII
DTPGHVDFTIEVERSLRVLDGAVCVLDSNQGVEPQTETVWRQGDKYKVPRIVFANKMDKTGADFFKCLSDIVDRLGAKPV
AIQLPIGAENNFKGCIDLVTMKAIVWNDESLGAKFDHVEIPAELADQAKEYREKLVEAAVELDDDALTAFLDGKEPDEAT
LKQLIRKAVLTGAFYPVLCGSAFKNKGVQPLLDAVVDYLPSPLDVPAIKGTDDRGNEVVRKADDKEPLSLLAFKIMDDPF
VGTITFCRIYSGILTSGTGVVNSTREKKERIGRMLLMHANNREDIKEAYAGDIVALAGLKEARTGDTLCDPNNQVILEKM
EFPEPVIEIAIEPKSKADQEKLGIALAKLAAEDPSFRVSTDHESGQTILKGMGELHLDIKVDILKRTYKVDANIGAPQVA
FRERITKPANVDYTHKKQTGGTGQFAAVSLVVEPNEPGGGYVFESKIVGGAVPKEYIPGVEKGIESVLGAGVVAGFPVVD
VKVALVDGKYHDVDSSALAFEIASRAAFREALQKGKSVLLEPIMKVEVVTPEDYTGSVIGDLNSRRGQIQGQDMRGNANV
INAMVPLMNMFGYVNNLRSMSQGRATFTMQFSHYAEAPANVSAEVQKKFA
>Mature_689_residues
PRQHAIEDYRNFGIMAHIDAGKTTTTERILYYTGKSHKIGEVHEGAATMDWMEQEQERGITITSAATTAFWNGKRLNIID
TPGHVDFTIEVERSLRVLDGAVCVLDSNQGVEPQTETVWRQGDKYKVPRIVFANKMDKTGADFFKCLSDIVDRLGAKPVA
IQLPIGAENNFKGCIDLVTMKAIVWNDESLGAKFDHVEIPAELADQAKEYREKLVEAAVELDDDALTAFLDGKEPDEATL
KQLIRKAVLTGAFYPVLCGSAFKNKGVQPLLDAVVDYLPSPLDVPAIKGTDDRGNEVVRKADDKEPLSLLAFKIMDDPFV
GTITFCRIYSGILTSGTGVVNSTREKKERIGRMLLMHANNREDIKEAYAGDIVALAGLKEARTGDTLCDPNNQVILEKME
FPEPVIEIAIEPKSKADQEKLGIALAKLAAEDPSFRVSTDHESGQTILKGMGELHLDIKVDILKRTYKVDANIGAPQVAF
RERITKPANVDYTHKKQTGGTGQFAAVSLVVEPNEPGGGYVFESKIVGGAVPKEYIPGVEKGIESVLGAGVVAGFPVVDV
KVALVDGKYHDVDSSALAFEIASRAAFREALQKGKSVLLEPIMKVEVVTPEDYTGSVIGDLNSRRGQIQGQDMRGNANVI
NAMVPLMNMFGYVNNLRSMSQGRATFTMQFSHYAEAPANVSAEVQKKFA

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=695, Percent_Identity=43.5971223021583, Blast_Score=545, Evalue=1e-155,
Organism=Homo sapiens, GI19923640, Length=708, Percent_Identity=41.1016949152542, Blast_Score=469, Evalue=1e-132,
Organism=Homo sapiens, GI25306283, Length=448, Percent_Identity=44.8660714285714, Blast_Score=331, Evalue=2e-90,
Organism=Homo sapiens, GI25306287, Length=287, Percent_Identity=53.3101045296167, Blast_Score=280, Evalue=4e-75,
Organism=Homo sapiens, GI4503483, Length=496, Percent_Identity=25.2016129032258, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI157426893, Length=141, Percent_Identity=39.7163120567376, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI94966754, Length=167, Percent_Identity=34.1317365269461, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI310132016, Length=124, Percent_Identity=36.2903225806452, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI310110807, Length=124, Percent_Identity=36.2903225806452, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI310123363, Length=124, Percent_Identity=36.2903225806452, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1789738, Length=698, Percent_Identity=59.7421203438395, Blast_Score=838, Evalue=0.0,
Organism=Escherichia coli, GI1790835, Length=491, Percent_Identity=28.3095723014257, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI48994988, Length=136, Percent_Identity=42.6470588235294, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1788922, Length=155, Percent_Identity=38.0645161290323, Blast_Score=99, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17533571, Length=702, Percent_Identity=40.8831908831909, Blast_Score=504, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17556745, Length=720, Percent_Identity=30.4166666666667, Blast_Score=318, Evalue=6e-87,
Organism=Caenorhabditis elegans, GI17557151, Length=198, Percent_Identity=37.3737373737374, Blast_Score=103, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17506493, Length=479, Percent_Identity=25.4697286012526, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=34.3283582089552, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=34.3283582089552, Blast_Score=76, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=689, Percent_Identity=42.2351233671988, Blast_Score=534, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6322359, Length=782, Percent_Identity=34.0153452685422, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6323320, Length=156, Percent_Identity=37.1794871794872, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324707, Length=151, Percent_Identity=38.4105960264901, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320593, Length=151, Percent_Identity=38.4105960264901, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=145, Percent_Identity=35.1724137931034, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24582462, Length=697, Percent_Identity=43.4720229555237, Blast_Score=560, Evalue=1e-159,
Organism=Drosophila melanogaster, GI221458488, Length=704, Percent_Identity=35.7954545454545, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24585711, Length=831, Percent_Identity=24.9097472924188, Blast_Score=165, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24585713, Length=831, Percent_Identity=24.9097472924188, Blast_Score=165, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24585709, Length=831, Percent_Identity=24.9097472924188, Blast_Score=165, Evalue=1e-40,
Organism=Drosophila melanogaster, GI78706572, Length=157, Percent_Identity=36.3057324840764, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI28574573, Length=146, Percent_Identity=34.2465753424658, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 75439; Mature: 75308

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRQHAIEDYRNFGIMAHIDAGKTTTTERILYYTGKSHKIGEVHEGAATMDWMEQEQERG
CCCCHHHHHHHCCCEEEEECCCCCCCCCEEEEEECCCCCCCCHHCCCHHHHHHHHHHHCC
ITITSAATTAFWNGKRLNIIDTPGHVDFTIEVERSLRVLDGAVCVLDSNQGVEPQTETVW
EEEEECCEEEEECCCEEEEEECCCCEEEEEEECCCCEEECCEEEEEECCCCCCCCHHHHH
RQGDKYKVPRIVFANKMDKTGADFFKCLSDIVDRLGAKPVAIQLPIGAENNFKGCIDLVT
HCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHH
MKAIVWNDESLGAKFDHVEIPAELADQAKEYREKLVEAAVELDDDALTAFLDGKEPDEAT
HHHEEECCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHH
LKQLIRKAVLTGAFYPVLCGSAFKNKGVQPLLDAVVDYLPSPLDVPAIKGTDDRGNEVVR
HHHHHHHHHHHCCHHHHHCCHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHH
KADDKEPLSLLAFKIMDDPFVGTITFCRIYSGILTSGTGVVNSTREKKERIGRMLLMHAN
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEEEEECC
NREDIKEAYAGDIVALAGLKEARTGDTLCDPNNQVILEKMEFPEPVIEIAIEPKSKADQE
CHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCHH
KLGIALAKLAAEDPSFRVSTDHESGQTILKGMGELHLDIKVDILKRTYKVDANIGAPQVA
HHHHHHHHHHCCCCCEEEECCCCCCHHHHHCCCCEEEEEEEEEEHHEEEECCCCCCCHHH
FRERITKPANVDYTHKKQTGGTGQFAAVSLVVEPNEPGGGYVFESKIVGGAVPKEYIPGV
HHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEECCCCCCCCCHHHCCCH
EKGIESVLGAGVVAGFPVVDVKVALVDGKYHDVDSSALAFEIASRAAFREALQKGKSVLL
HHHHHHHHHCHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EPIMKVEVVTPEDYTGSVIGDLNSRRGQIQGQDMRGNANVINAMVPLMNMFGYVNNLRSM
CCCCEEEEECCCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SQGRATFTMQFSHYAEAPANVSAEVQKKFA
HCCCEEEEEEEHHHCCCCCCCCHHHHHHCC
>Mature Secondary Structure 
PRQHAIEDYRNFGIMAHIDAGKTTTTERILYYTGKSHKIGEVHEGAATMDWMEQEQERG
CCCHHHHHHHCCCEEEEECCCCCCCCCEEEEEECCCCCCCCHHCCCHHHHHHHHHHHCC
ITITSAATTAFWNGKRLNIIDTPGHVDFTIEVERSLRVLDGAVCVLDSNQGVEPQTETVW
EEEEECCEEEEECCCEEEEEECCCCEEEEEEECCCCEEECCEEEEEECCCCCCCCHHHHH
RQGDKYKVPRIVFANKMDKTGADFFKCLSDIVDRLGAKPVAIQLPIGAENNFKGCIDLVT
HCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHH
MKAIVWNDESLGAKFDHVEIPAELADQAKEYREKLVEAAVELDDDALTAFLDGKEPDEAT
HHHEEECCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHH
LKQLIRKAVLTGAFYPVLCGSAFKNKGVQPLLDAVVDYLPSPLDVPAIKGTDDRGNEVVR
HHHHHHHHHHHCCHHHHHCCHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHH
KADDKEPLSLLAFKIMDDPFVGTITFCRIYSGILTSGTGVVNSTREKKERIGRMLLMHAN
CCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHEEEEECC
NREDIKEAYAGDIVALAGLKEARTGDTLCDPNNQVILEKMEFPEPVIEIAIEPKSKADQE
CHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCHH
KLGIALAKLAAEDPSFRVSTDHESGQTILKGMGELHLDIKVDILKRTYKVDANIGAPQVA
HHHHHHHHHHCCCCCEEEECCCCCCHHHHHCCCCEEEEEEEEEEHHEEEECCCCCCCHHH
FRERITKPANVDYTHKKQTGGTGQFAAVSLVVEPNEPGGGYVFESKIVGGAVPKEYIPGV
HHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEECCCCCCCCCHHHCCCH
EKGIESVLGAGVVAGFPVVDVKVALVDGKYHDVDSSALAFEIASRAAFREALQKGKSVLL
HHHHHHHHHCHHHCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EPIMKVEVVTPEDYTGSVIGDLNSRRGQIQGQDMRGNANVINAMVPLMNMFGYVNNLRSM
CCCCEEEEECCCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
SQGRATFTMQFSHYAEAPANVSAEVQKKFA
HCCCEEEEEEEHHHCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]