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The map label for this gene is tufB [H]

Identifier: 146340055

GI number: 146340055

Start: 3271707

End: 3272897

Strand: Direct

Name: tufB [H]

Synonym: BRADO3064

Alternate gene names: 146340055

Gene position: 3271707-3272897 (Clockwise)

Preceding gene: 146340054

Following gene: 146340056

Centisome position: 43.88

GC content: 63.81

Gene sequence:

>1191_bases
ATGGCCAAAGCTAAGTTTGAACGTAACAAGCCCCACTGCAACATCGGCACCATCGGTCACGTCGACCATGGCAAGACGTC
GCTGACGGCTGCCATCACCAAGATCCTGGCCGAGACGGGCGGTGCGACGTTCACTGCCTACGACCAGATCGACAAGGCGC
CCGAAGAGAAGGCCCGCGGCATCACCATCTCGACCGCCCACGTCGAGTACGAGACGCAGAACCGCCACTACGCGCACGTC
GACTGCCCCGGCCACGCCGACTACGTGAAGAACATGATCACCGGCGCCGCCCAGATGGACGGCGCGATCCTGGTCGTGTC
GGCTGCCGACGGCCCGATGCCGCAGACCCGCGAGCACATCCTGCTCGCCCGCCAGGTCGGTGTTCCCGCGCTGGTCGTGT
TCCTGAACAAGTGCGACATGGTCGACGATCCGGAGCTGCTCGAGCTGGTCGAGCTCGAGGTTCGCGAGCTGCTCTCGAAG
TATGAATTCCCGGGCGACAAGATCCCGATCATCAAGGGCTCGGCGCTCGCCGCTCTCGAAGATTCGGACAAGAAGCTCGG
CCACGACGCCATCCTCGAGCTGATGCGCAATGTCGACGAGTACATTCCGCAGCCGGAGCGTCCGATCGACCAGCCCTTCC
TGATGCCGGTCGAAGACGTGTTCTCGATCTCGGGCCGCGGCACCGTGGTGACCGGTCGTGTCGAGCGCGGCGTGATCAAG
GTCGGTGAGGAAATCGAGATCGTCGGCATCCGTCCGACCCAGAAGACCACCGTCACCGGCGTCGAAATGTTCCGCAAGCT
GCTCGATCAGGGCCAGGCTGGCGACAACATCGGTGCGCTGCTCCGCGGCACCAAGCGTGAGGACGTCGAGCGCGGCCAGG
TTCTCTGCAAGCCGGGTTCGGTCAAGCCGCACACCAAGTTCAAGGCCGAGGCGTACATCCTCACCAAGGAAGAGGGCGGC
CGTCACACTCCGTTCTTCACCAACTATCGTCCGCAGTTCTACTTCCGCACGACCGACGTGACCGGCGTCGTTCACCTGCC
GGAAGGGACCGAGATGGTGATGCCGGGCGACAACATCGCGATGGAAGTGCACCTGATCGTGCCGATCGCGATGGAAGAGA
AGCTGCGCTTCGCCATCCGTGAAGGCGGCCGCACCGTCGGCGCCGGCGTCGTCGCCTCGATCATCGAGTAA

Upstream 100 bases:

>100_bases
CTCGCACTACGCTGAAGCGCCGGCCAACGTGTCGGCTGAAGTCCAGAAGAAGTTTGCCTGATTGTCGTTAGCTCAAGTTA
GCGACTGAACGGAGAGTCAA

Downstream 100 bases:

>100_bases
CAACACGAATAGGGAATGGCGGGTGGCGAATGGTGATCCATTCGCCGTTCGCTACTCGCCACTCACTACTCACAGAAAGA
TACGGCAATGAACGGCCAAA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKAKFERNKPHCNIGTIGHVDHGKTSLTAAITKILAETGGATFTAYDQIDKAPEEKARGITISTAHVEYETQNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFLNKCDMVDDPELLELVELEVRELLSK
YEFPGDKIPIIKGSALAALEDSDKKLGHDAILELMRNVDEYIPQPERPIDQPFLMPVEDVFSISGRGTVVTGRVERGVIK
VGEEIEIVGIRPTQKTTVTGVEMFRKLLDQGQAGDNIGALLRGTKREDVERGQVLCKPGSVKPHTKFKAEAYILTKEEGG
RHTPFFTNYRPQFYFRTTDVTGVVHLPEGTEMVMPGDNIAMEVHLIVPIAMEEKLRFAIREGGRTVGAGVVASIIE

Sequences:

>Translated_396_residues
MAKAKFERNKPHCNIGTIGHVDHGKTSLTAAITKILAETGGATFTAYDQIDKAPEEKARGITISTAHVEYETQNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFLNKCDMVDDPELLELVELEVRELLSK
YEFPGDKIPIIKGSALAALEDSDKKLGHDAILELMRNVDEYIPQPERPIDQPFLMPVEDVFSISGRGTVVTGRVERGVIK
VGEEIEIVGIRPTQKTTVTGVEMFRKLLDQGQAGDNIGALLRGTKREDVERGQVLCKPGSVKPHTKFKAEAYILTKEEGG
RHTPFFTNYRPQFYFRTTDVTGVVHLPEGTEMVMPGDNIAMEVHLIVPIAMEEKLRFAIREGGRTVGAGVVASIIE
>Mature_395_residues
AKAKFERNKPHCNIGTIGHVDHGKTSLTAAITKILAETGGATFTAYDQIDKAPEEKARGITISTAHVEYETQNRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVPALVVFLNKCDMVDDPELLELVELEVRELLSKY
EFPGDKIPIIKGSALAALEDSDKKLGHDAILELMRNVDEYIPQPERPIDQPFLMPVEDVFSISGRGTVVTGRVERGVIKV
GEEIEIVGIRPTQKTTVTGVEMFRKLLDQGQAGDNIGALLRGTKREDVERGQVLCKPGSVKPHTKFKAEAYILTKEEGGR
HTPFFTNYRPQFYFRTTDVTGVVHLPEGTEMVMPGDNIAMEVHLIVPIAMEEKLRFAIREGGRTVGAGVVASIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=57.6142131979695, Blast_Score=484, Evalue=1e-137,
Organism=Homo sapiens, GI223555963, Length=445, Percent_Identity=30.1123595505618, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI5729864, Length=445, Percent_Identity=30.1123595505618, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI4503471, Length=443, Percent_Identity=30.4740406320542, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI4503475, Length=444, Percent_Identity=29.5045045045045, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI194018522, Length=323, Percent_Identity=29.7213622291022, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI194018520, Length=323, Percent_Identity=29.7213622291022, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI194097354, Length=323, Percent_Identity=29.7213622291022, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI46094014, Length=323, Percent_Identity=30.030959752322, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI54607086, Length=316, Percent_Identity=28.4810126582279, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI4503507, Length=269, Percent_Identity=28.2527881040892, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI157426893, Length=307, Percent_Identity=27.3615635179153, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI53729339, Length=234, Percent_Identity=29.4871794871795, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI53729337, Length=234, Percent_Identity=29.4871794871795, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=76.2025316455696, Blast_Score=602, Evalue=1e-173,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=76.2025316455696, Blast_Score=601, Evalue=1e-173,
Organism=Escherichia coli, GI2367247, Length=379, Percent_Identity=31.3984168865435, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI48994988, Length=241, Percent_Identity=31.9502074688797, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789108, Length=342, Percent_Identity=26.3157894736842, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17556456, Length=400, Percent_Identity=52.5, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=42.3772609819121, Blast_Score=328, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI17552884, Length=444, Percent_Identity=30.8558558558559, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17569207, Length=444, Percent_Identity=30.8558558558559, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI32566303, Length=420, Percent_Identity=29.7619047619048, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI115532067, Length=437, Percent_Identity=27.6887871853547, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=432, Percent_Identity=27.7777777777778, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=298, Percent_Identity=28.5234899328859, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI32566629, Length=343, Percent_Identity=28.5714285714286, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=38.7755102040816, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17509919, Length=274, Percent_Identity=26.6423357664234, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=234, Percent_Identity=27.3504273504274, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=67.8391959798995, Blast_Score=545, Evalue=1e-156,
Organism=Saccharomyces cerevisiae, GI6325337, Length=442, Percent_Identity=30.5429864253394, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=442, Percent_Identity=30.5429864253394, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=351, Percent_Identity=26.7806267806268, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6320377, Length=345, Percent_Identity=28.1159420289855, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=268, Percent_Identity=28.3582089552239, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=181, Percent_Identity=32.5966850828729, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=59.0330788804071, Blast_Score=496, Evalue=1e-140,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=59.0330788804071, Blast_Score=496, Evalue=1e-140,
Organism=Drosophila melanogaster, GI19921738, Length=384, Percent_Identity=50.2604166666667, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI45553807, Length=449, Percent_Identity=28.9532293986637, Blast_Score=158, Evalue=6e-39,
Organism=Drosophila melanogaster, GI45553816, Length=449, Percent_Identity=28.9532293986637, Blast_Score=158, Evalue=6e-39,
Organism=Drosophila melanogaster, GI24651721, Length=449, Percent_Identity=28.9532293986637, Blast_Score=158, Evalue=6e-39,
Organism=Drosophila melanogaster, GI17864154, Length=449, Percent_Identity=28.9532293986637, Blast_Score=158, Evalue=6e-39,
Organism=Drosophila melanogaster, GI24652838, Length=449, Percent_Identity=29.1759465478842, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI17137572, Length=449, Percent_Identity=29.1759465478842, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=32.3432343234323, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI17137380, Length=341, Percent_Identity=29.9120234604106, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI45550900, Length=337, Percent_Identity=29.3768545994065, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24647046, Length=280, Percent_Identity=27.5, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24647048, Length=280, Percent_Identity=27.5, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI78706572, Length=310, Percent_Identity=26.4516129032258, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43484; Mature: 43352

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERNKPHCNIGTIGHVDHGKTSLTAAITKILAETGGATFTAYDQIDKAPEEKARG
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHCC
ITISTAHVEYETQNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPALVVFLNKCDMVDDPELLELVELEVRELLSKYEFPGDKIPIIKGSALAALE
HHHHHCCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEC
DSDKKLGHDAILELMRNVDEYIPQPERPIDQPFLMPVEDVFSISGRGTVVTGRVERGVIK
CCCHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECCCCCCEEE
VGEEIEIVGIRPTQKTTVTGVEMFRKLLDQGQAGDNIGALLRGTKREDVERGQVLCKPGS
CCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHCCCCEEECCCC
VKPHTKFKAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGVVHLPEGTEMVMPGDNIA
CCCCCCEEEEEEEEEECCCCCCCCEEECCCCEEEEEEECCEEEEECCCCCEEEECCCCEE
MEVHLIVPIAMEEKLRFAIREGGRTVGAGVVASIIE
EEEEEEEEEEHHHHHHHHHHHCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKAKFERNKPHCNIGTIGHVDHGKTSLTAAITKILAETGGATFTAYDQIDKAPEEKARG
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHHHCC
ITISTAHVEYETQNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPALVVFLNKCDMVDDPELLELVELEVRELLSKYEFPGDKIPIIKGSALAALE
HHHHHCCCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEC
DSDKKLGHDAILELMRNVDEYIPQPERPIDQPFLMPVEDVFSISGRGTVVTGRVERGVIK
CCCHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCEEEECCCCCCEEE
VGEEIEIVGIRPTQKTTVTGVEMFRKLLDQGQAGDNIGALLRGTKREDVERGQVLCKPGS
CCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHCCCCEEECCCC
VKPHTKFKAEAYILTKEEGGRHTPFFTNYRPQFYFRTTDVTGVVHLPEGTEMVMPGDNIA
CCCCCCEEEEEEEEEECCCCCCCCEEECCCCEEEEEEECCEEEEECCCCCEEEECCCCEE
MEVHLIVPIAMEEKLRFAIREGGRTVGAGVVASIIE
EEEEEEEEEEHHHHHHHHHHHCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA