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The map label for this gene is nasD [H]

Identifier: 146339486

GI number: 146339486

Start: 2636660

End: 2637841

Strand: Direct

Name: nasD [H]

Synonym: BRADO2475

Alternate gene names: 146339486

Gene position: 2636660-2637841 (Clockwise)

Preceding gene: 146339485

Following gene: 146339487

Centisome position: 35.36

GC content: 68.95

Gene sequence:

>1182_bases
ATGGCCGCCGCGAAGCTGGTCGAGGAACTGTCGCAGACTGCGCTCGGCCGCTACGCCATCGCCGTGATCGGCGAGGAGCC
GCGGCTCGCCTATAACCGCGTGCTGCTGTCCTCGGTGCTGGCGGGCGAGGCGGCCTCGCACGAGATCGAGCTCAAGCCGG
CCGCGTGGTGGCGCGAGCGCGGCGTCACGTTGAAATATGGCGCGCGCGTCACCGGCATCGATGTCGGCCGCCGCGAGCTC
CAGATCGAGAACGACGAGAACGTGATGTTCTCGCGTCTGGTGCTGGCCACCGGCTCGCTGCCGCTGCGGCTGCCGGTGCC
CGGCAGCGATCTGCCGGGCGTGCACACCTTCCGTGACAGCCGTGATACCGACGTGCTGCTCAGCCTCGCTGCGAAGAAAG
CGCGCGTCGTGGTCGTCGGCGGCGGATTGCTGGGGCTGGAGGCGGCCTACGGTCTGGCCCGCGCCGGCGCCAAGGTGACG
CTGCTGCACCTGATGGACCGGCTGATGGAGCGCCAGCTCGATGCGCCCGCCGCCGCGCTGCTGAAAGTGCTGGTCGAGCG
CAAGGGCATCGAGGTTCTGCTGCAGGCGAGCACGGCCGCGATCCATGGCAGCGCCGGAGTCGAGGGTGTCGAGCTCGCCG
ATGGGCGGCGCATCGCCGCGGACGCCGTGATCTTCGCTGCCGGCATCAAGCCCAATGTGGCGCTCGCCAAGGAGGCCGGG
CTTGCCGTCAATCGCGGCATCGTCATCGACGATGTCATGCAGACCTCGGCTCCGGGAATCTTCGCGCTCGGCGAATGCGC
CGAGCATCGCGGCACCTGCTACGGCCTGGTCGAGCCCGCCTACGAGCAGGCACGCGTTCTGGCGCGGCATCTTGCAGGCC
GCAAGGCCGCCTATGGCGGCAGTGTCGTGTCAACGAATTTGAAAGTCTCGGGTGTTGCGGTGTTTTCGGCCGGCGACTTC
ATGGGCGCCGGCGGCAGCGAGGCGATCGTGCTCAACGATGCCAGGCGTGGCATCTACAAGAAGCTGGTCATCGCCGACGG
ACGGCTCACCGGTGCCGTGCTGATCGGTGAGACCCAGGATGCGCTCTGGTATCGCGATCTGATCCGCAGCCGGACGCCGG
TCGCGGCGATCCGGCACGAGATGATGTTCGGCCGCGCGGCGGCGCGGCCCAATGCGGCGTGA

Upstream 100 bases:

>100_bases
ATGGCACGCTGTCCGGCTTCATGATCGCGGAAGCCTATGGCAAGGCCCAGGCGGCGGAGTAGTTGCGTTGAGTGAGCCGC
TCGTCATCGTCGGCAACGGC

Downstream 100 bases:

>100_bases
GAGAGGGAGCTCGCACGATGACGAGCGTTGATCCGAAGTTGCGCACAGTGCGTACCGCCTGTCCGTATTGTGGCGTCGGC
TGCGGCGTGCTGGCGACGCC

Product: nitrite reductase [NAD(P)H] large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MAAAKLVEELSQTALGRYAIAVIGEEPRLAYNRVLLSSVLAGEAASHEIELKPAAWWRERGVTLKYGARVTGIDVGRREL
QIENDENVMFSRLVLATGSLPLRLPVPGSDLPGVHTFRDSRDTDVLLSLAAKKARVVVVGGGLLGLEAAYGLARAGAKVT
LLHLMDRLMERQLDAPAAALLKVLVERKGIEVLLQASTAAIHGSAGVEGVELADGRRIAADAVIFAAGIKPNVALAKEAG
LAVNRGIVIDDVMQTSAPGIFALGECAEHRGTCYGLVEPAYEQARVLARHLAGRKAAYGGSVVSTNLKVSGVAVFSAGDF
MGAGGSEAIVLNDARRGIYKKLVIADGRLTGAVLIGETQDALWYRDLIRSRTPVAAIRHEMMFGRAAARPNAA

Sequences:

>Translated_393_residues
MAAAKLVEELSQTALGRYAIAVIGEEPRLAYNRVLLSSVLAGEAASHEIELKPAAWWRERGVTLKYGARVTGIDVGRREL
QIENDENVMFSRLVLATGSLPLRLPVPGSDLPGVHTFRDSRDTDVLLSLAAKKARVVVVGGGLLGLEAAYGLARAGAKVT
LLHLMDRLMERQLDAPAAALLKVLVERKGIEVLLQASTAAIHGSAGVEGVELADGRRIAADAVIFAAGIKPNVALAKEAG
LAVNRGIVIDDVMQTSAPGIFALGECAEHRGTCYGLVEPAYEQARVLARHLAGRKAAYGGSVVSTNLKVSGVAVFSAGDF
MGAGGSEAIVLNDARRGIYKKLVIADGRLTGAVLIGETQDALWYRDLIRSRTPVAAIRHEMMFGRAAARPNAA
>Mature_392_residues
AAAKLVEELSQTALGRYAIAVIGEEPRLAYNRVLLSSVLAGEAASHEIELKPAAWWRERGVTLKYGARVTGIDVGRRELQ
IENDENVMFSRLVLATGSLPLRLPVPGSDLPGVHTFRDSRDTDVLLSLAAKKARVVVVGGGLLGLEAAYGLARAGAKVTL
LHLMDRLMERQLDAPAAALLKVLVERKGIEVLLQASTAAIHGSAGVEGVELADGRRIAADAVIFAAGIKPNVALAKEAGL
AVNRGIVIDDVMQTSAPGIFALGECAEHRGTCYGLVEPAYEQARVLARHLAGRKAAYGGSVVSTNLKVSGVAVFSAGDFM
GAGGSEAIVLNDARRGIYKKLVIADGRLTGAVLIGETQDALWYRDLIRSRTPVAAIRHEMMFGRAAARPNAA

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI226437568, Length=278, Percent_Identity=26.2589928057554, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI65787454, Length=278, Percent_Identity=26.2589928057554, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI21389617, Length=278, Percent_Identity=26.2589928057554, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI22202629, Length=257, Percent_Identity=24.5136186770428, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI4757732, Length=257, Percent_Identity=24.5136186770428, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1789765, Length=368, Percent_Identity=34.7826086956522, Blast_Score=177, Evalue=8e-46,
Organism=Escherichia coli, GI1789065, Length=291, Percent_Identity=30.2405498281787, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788892, Length=248, Percent_Identity=31.0483870967742, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786307, Length=247, Percent_Identity=27.5303643724696, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=251, Percent_Identity=30.6772908366534, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24639257, Length=299, Percent_Identity=26.4214046822742, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI281359715, Length=299, Percent_Identity=26.4214046822742, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI281359713, Length=299, Percent_Identity=26.4214046822742, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24639250, Length=299, Percent_Identity=26.4214046822742, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI18543267, Length=299, Percent_Identity=26.4214046822742, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24639252, Length=299, Percent_Identity=26.4214046822742, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24585130, Length=271, Percent_Identity=21.4022140221402, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24581020, Length=197, Percent_Identity=27.9187817258883, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI28573993, Length=197, Percent_Identity=27.9187817258883, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 41507; Mature: 41375

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAAKLVEELSQTALGRYAIAVIGEEPRLAYNRVLLSSVLAGEAASHEIELKPAAWWRER
CCHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHC
GVTLKYGARVTGIDVGRRELQIENDENVMFSRLVLATGSLPLRLPVPGSDLPGVHTFRDS
CCEEEECCEEEEEECCCEEEEEECCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCC
RDTDVLLSLAAKKARVVVVGGGLLGLEAAYGLARAGAKVTLLHLMDRLMERQLDAPAAAL
CCHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCCHHHH
LKVLVERKGIEVLLQASTAAIHGSAGVEGVELADGRRIAADAVIFAAGIKPNVALAKEAG
HHHHHHCCCCEEEEEECCEEEECCCCCCCEEECCCCEEECCEEEEEECCCCCEEEHHHCC
LAVNRGIVIDDVMQTSAPGIFALGECAEHRGTCYGLVEPAYEQARVLARHLAGRKAAYGG
CEECCCEEEEHHHHHCCCCEEEEHHHHHHCCCEEEECCHHHHHHHHHHHHHCCCCCCCCC
SVVSTNLKVSGVAVFSAGDFMGAGGSEAIVLNDARRGIYKKLVIADGRLTGAVLIGETQD
CEEECCEEEEEEEEEECCCCCCCCCCCEEEEECHHHCHHHEEEEECCCEEEEEEEECCCH
ALWYRDLIRSRTPVAAIRHEMMFGRAAARPNAA
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AAAKLVEELSQTALGRYAIAVIGEEPRLAYNRVLLSSVLAGEAASHEIELKPAAWWRER
CHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHC
GVTLKYGARVTGIDVGRRELQIENDENVMFSRLVLATGSLPLRLPVPGSDLPGVHTFRDS
CCEEEECCEEEEEECCCEEEEEECCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCC
RDTDVLLSLAAKKARVVVVGGGLLGLEAAYGLARAGAKVTLLHLMDRLMERQLDAPAAAL
CCHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHCCCCHHHH
LKVLVERKGIEVLLQASTAAIHGSAGVEGVELADGRRIAADAVIFAAGIKPNVALAKEAG
HHHHHHCCCCEEEEEECCEEEECCCCCCCEEECCCCEEECCEEEEEECCCCCEEEHHHCC
LAVNRGIVIDDVMQTSAPGIFALGECAEHRGTCYGLVEPAYEQARVLARHLAGRKAAYGG
CEECCCEEEEHHHHHCCCCEEEEHHHHHHCCCEEEECCHHHHHHHHHHHHHCCCCCCCCC
SVVSTNLKVSGVAVFSAGDFMGAGGSEAIVLNDARRGIYKKLVIADGRLTGAVLIGETQD
CEEECCEEEEEEEEEECCCCCCCCCCCEEEEECHHHCHHHEEEEECCCEEEEEEEECCCH
ALWYRDLIRSRTPVAAIRHEMMFGRAAARPNAA
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]