| Definition | Bradyrhizobium sp. ORS278 chromosome, complete genome. |
|---|---|
| Accession | NC_009445 |
| Length | 7,456,587 |
Click here to switch to the map view.
The map label for this gene is nasA [H]
Identifier: 146339487
GI number: 146339487
Start: 2637838
End: 2640552
Strand: Direct
Name: nasA [H]
Synonym: BRADO2476
Alternate gene names: 146339487
Gene position: 2637838-2640552 (Clockwise)
Preceding gene: 146339486
Following gene: 146339488
Centisome position: 35.38
GC content: 68.8
Gene sequence:
>2715_bases GTGAGAGAGGGAGCTCGCACGATGACGAGCGTTGATCCGAAGTTGCGCACAGTGCGTACCGCCTGTCCGTATTGTGGCGT CGGCTGCGGCGTGCTGGCGACGCCCGATGGCAGCGGCGGCGCGGCGATCTCGGGTGATCCCGTGCATCCGGCCAATCTCG GCCGCCTGTGCTCCAAGGGCTCGGCCCTTGGCGAGACGCTGGGGCTGGAGAACCGGCTGCTGTATCCGATGGTCCGCTGC AAGCACGGCATGGAGCGGGTGGCCTGGAGCGACGCGCTGGACCACGTCGCCACGCGGCTGAGGCACATCGTCGCCCGCGA CGGCGAGGACGCGGTCGCGTTCTATCTCTCCGGCCAGATGCTGACCGAGGACTATTACGTCGCCAACAAGCTGATGAAGG GCTTTCTCGGCTCGGCCAATGTCGACACCAATTCGCGGCTGTGCATGGCGTCCTCGGTCGCCGGCCATCGCCGCGCGTTC GGCGCCGACACGGTGCCCGGCACCTATGAGGATCTCGACCAGGCCGACCTGCTGGTGTTCGTCGGCAGCAACGCGGCCTG GTGTCATCCGGTGCTGTATCAGCGCATGCTGGCGAACAAGCAGAGCCGCGGCGCGAAAATCGTCGTCATCGATCCGCGCC GCACCGATACGGCGAGCGAAGCTGATCTGTTCCTCGGGCTCAAGCCCGGCACGGATGCCGCGCTGTTCTCGGGCCTGCTG GTGCATCTCGCCGACAGCGGTGCGCTCGATCGCGACTACATCGCGCGTCACACGTCCGGCTTCGACGACACGCTCGCGCG CGCCCGCCACCTCGCCGGCAGCGCCGGCGCCACCGCGCTTGCCACCGGCCTGCCGGAAGCCGACGTCGCCGCCTTCTTCA AGCTGTTCCGCGACACGCCGAAGGTCGTCACGCTGTACTCGCAGGGTGTCAACCAGTCGGCGCAGGGCACCGACAAGGTC AATGCGATCGTCAACTGCCATCTCGCCACCGCGCGCATCGGCAAGCCCGGCATGGGACCGTTCTCCCTGACCGGACAGCC CAACGCGATGGGCGGCCGCGAGGTCGGCGGTCTCGCCAATCAGCTCGCCGCGCATATGAGCTTCACGCCGCCGGACATCG ATCGCGTCCGTCGCTTCTGGAAGGCATCCGACATCGCCACCCATGAGGGGCTGAAGGCGGTGCAGATGTTCGAGGCGATC GCGCGCGGCCAGATCAAGGCGCTGTGGGTGATCGGCACCAATCCGGCGGTGTCGCTGCCGGATGCCGATCTGGTGCGGTC GGCGCTGAAGAAGCTCGAGCTGTTCGTCATCTCCGAGAACGTCCGCTCCAACGACACCGTGGATGTCGGCGCCCATGTGC TGCTGCCGGCGCAGGCCTGGGGCGAGAAGTCGGGCACCGTCACCAATTCCGAGCGCCGCATCTCGCGCCAGCGCGCCTTC ATGCCGCCGGTGGGGGAGGCGAAGCCGGACTGGTGGATCCTGAGCGAGGTCGGCAAGCGGCTCGGCCACGCGGCGGCATT CAACTATACCTCCGCCGCCGACGTGTTCCGCGAACACGCCGCGCTGTCGGCCTTCGAGAACGACGGCAGCCGCGACTTCG ACATCGGCGGCCTCGCGACTCTGTCGGACGACGAGTACGACACGATGGCGCCGGTGCAGTGGCCGGTGCGCGCCGGCAAG CCGCCGTCGCCGCGCTTCTTCGCCGACGGCGCCTTCTACACCAACGACCGCAAGGCGCGCTTCGTCGCGCCGGAGGTGCC GGCGCTACGCAGCGAGACCACGGCCGCGCGACCGCTGCGGCTGAACACCGGCCGCATCCGCGACCAGTGGCACACGATGA CGCGCACGGGCCTGAGCCCGCGTCTCGGCGCGCATCTGCCGGAGCCGTTCGTCGAGGTGCACCCGGACGACGCGCTGCGC TACGGCCTGATGCCGGACGGCTTCGCCAAGGTGACCACCGATCACGGCCAGTGCATCCTGAAGGTGGTCGTCAGCGAGCG CCAGCAGCGCGGCATGCTGTTCGCGCCGATCCATTGGAACGCGACCAACTCATCGCATGGCCGCGTCGGCGCGATGGTCT CCTCCCACACCGATCCGTTCTCCGGCCAGCCGGAGGCCAAGGCCACGCCGGCCTCGATCAGTCCTTACGAGTACGTGTTC CGCGGCTTCATCCTGTCGCGCACCGAACTGACGCTGCCGTCGAACCTGTGGTGGGTGCGTGCGACCATCCCGGGCGGCTT CGGTTATCTGTTCGCCGACAATGGTGACCTCATGCGCTGGCCCGGCTGGTTCAAGGCCCGCGCCACGGACGACATCGCCG ACTACATGGACGTCGGCGGCGGCGTCTATCGTGCCGCGTCGTTCGACGGCGATCGCATCGCAACGTCCGTGTTCGTCGGC CCCGGCCATGACGCGGGCGACTGGAACGTCGTGAAGACGATGTTCGCCGCCGACCATCTCGACGACGAGCAGCGCCGCAT GCTGCTGTCCGGCCGCGCGCCGGATGGCACCGGCGGTGGCCCGATCGTCTGCGCCTGCTTCGGCGTCGGCCGCGACACGA TCTGCGATGCGATCGCCGCGGGCGCCCGCACGCCCGCAGAGATCGGCGCGAAGCTTAAGGCCGGCACCAATTGCGGCTCG TGCATCCCCGAGATGAAGCGCCTGATCGCGCAGGTGCCGGCGCAGGCGGAGGCATCCGTCAGAGTGGCGAGCTAG
Upstream 100 bases:
>100_bases ATGCGCTCTGGTATCGCGATCTGATCCGCAGCCGGACGCCGGTCGCGGCGATCCGGCACGAGATGATGTTCGGCCGCGCG GCGGCGCGGCCCAATGCGGC
Downstream 100 bases:
>100_bases CTCGGCACCGTCGTTGCGAGCGAAGGAATCCAGAGTCCCGCGACGGCTCTGGATTGCTTTGTCGCTTCGCTTCTCGCAAT GACGGCAGTGGCCGATCGAC
Product: nitrate reductase large subunit
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 904; Mature: 904
Protein sequence:
>904_residues MREGARTMTSVDPKLRTVRTACPYCGVGCGVLATPDGSGGAAISGDPVHPANLGRLCSKGSALGETLGLENRLLYPMVRC KHGMERVAWSDALDHVATRLRHIVARDGEDAVAFYLSGQMLTEDYYVANKLMKGFLGSANVDTNSRLCMASSVAGHRRAF GADTVPGTYEDLDQADLLVFVGSNAAWCHPVLYQRMLANKQSRGAKIVVIDPRRTDTASEADLFLGLKPGTDAALFSGLL VHLADSGALDRDYIARHTSGFDDTLARARHLAGSAGATALATGLPEADVAAFFKLFRDTPKVVTLYSQGVNQSAQGTDKV NAIVNCHLATARIGKPGMGPFSLTGQPNAMGGREVGGLANQLAAHMSFTPPDIDRVRRFWKASDIATHEGLKAVQMFEAI ARGQIKALWVIGTNPAVSLPDADLVRSALKKLELFVISENVRSNDTVDVGAHVLLPAQAWGEKSGTVTNSERRISRQRAF MPPVGEAKPDWWILSEVGKRLGHAAAFNYTSAADVFREHAALSAFENDGSRDFDIGGLATLSDDEYDTMAPVQWPVRAGK PPSPRFFADGAFYTNDRKARFVAPEVPALRSETTAARPLRLNTGRIRDQWHTMTRTGLSPRLGAHLPEPFVEVHPDDALR YGLMPDGFAKVTTDHGQCILKVVVSERQQRGMLFAPIHWNATNSSHGRVGAMVSSHTDPFSGQPEAKATPASISPYEYVF RGFILSRTELTLPSNLWWVRATIPGGFGYLFADNGDLMRWPGWFKARATDDIADYMDVGGGVYRAASFDGDRIATSVFVG PGHDAGDWNVVKTMFAADHLDDEQRRMLLSGRAPDGTGGGPIVCACFGVGRDTICDAIAAGARTPAEIGAKLKAGTNCGS CIPEMKRLIAQVPAQAEASVRVAS
Sequences:
>Translated_904_residues MREGARTMTSVDPKLRTVRTACPYCGVGCGVLATPDGSGGAAISGDPVHPANLGRLCSKGSALGETLGLENRLLYPMVRC KHGMERVAWSDALDHVATRLRHIVARDGEDAVAFYLSGQMLTEDYYVANKLMKGFLGSANVDTNSRLCMASSVAGHRRAF GADTVPGTYEDLDQADLLVFVGSNAAWCHPVLYQRMLANKQSRGAKIVVIDPRRTDTASEADLFLGLKPGTDAALFSGLL VHLADSGALDRDYIARHTSGFDDTLARARHLAGSAGATALATGLPEADVAAFFKLFRDTPKVVTLYSQGVNQSAQGTDKV NAIVNCHLATARIGKPGMGPFSLTGQPNAMGGREVGGLANQLAAHMSFTPPDIDRVRRFWKASDIATHEGLKAVQMFEAI ARGQIKALWVIGTNPAVSLPDADLVRSALKKLELFVISENVRSNDTVDVGAHVLLPAQAWGEKSGTVTNSERRISRQRAF MPPVGEAKPDWWILSEVGKRLGHAAAFNYTSAADVFREHAALSAFENDGSRDFDIGGLATLSDDEYDTMAPVQWPVRAGK PPSPRFFADGAFYTNDRKARFVAPEVPALRSETTAARPLRLNTGRIRDQWHTMTRTGLSPRLGAHLPEPFVEVHPDDALR YGLMPDGFAKVTTDHGQCILKVVVSERQQRGMLFAPIHWNATNSSHGRVGAMVSSHTDPFSGQPEAKATPASISPYEYVF RGFILSRTELTLPSNLWWVRATIPGGFGYLFADNGDLMRWPGWFKARATDDIADYMDVGGGVYRAASFDGDRIATSVFVG PGHDAGDWNVVKTMFAADHLDDEQRRMLLSGRAPDGTGGGPIVCACFGVGRDTICDAIAAGARTPAEIGAKLKAGTNCGS CIPEMKRLIAQVPAQAEASVRVAS >Mature_904_residues MREGARTMTSVDPKLRTVRTACPYCGVGCGVLATPDGSGGAAISGDPVHPANLGRLCSKGSALGETLGLENRLLYPMVRC KHGMERVAWSDALDHVATRLRHIVARDGEDAVAFYLSGQMLTEDYYVANKLMKGFLGSANVDTNSRLCMASSVAGHRRAF GADTVPGTYEDLDQADLLVFVGSNAAWCHPVLYQRMLANKQSRGAKIVVIDPRRTDTASEADLFLGLKPGTDAALFSGLL VHLADSGALDRDYIARHTSGFDDTLARARHLAGSAGATALATGLPEADVAAFFKLFRDTPKVVTLYSQGVNQSAQGTDKV NAIVNCHLATARIGKPGMGPFSLTGQPNAMGGREVGGLANQLAAHMSFTPPDIDRVRRFWKASDIATHEGLKAVQMFEAI ARGQIKALWVIGTNPAVSLPDADLVRSALKKLELFVISENVRSNDTVDVGAHVLLPAQAWGEKSGTVTNSERRISRQRAF MPPVGEAKPDWWILSEVGKRLGHAAAFNYTSAADVFREHAALSAFENDGSRDFDIGGLATLSDDEYDTMAPVQWPVRAGK PPSPRFFADGAFYTNDRKARFVAPEVPALRSETTAARPLRLNTGRIRDQWHTMTRTGLSPRLGAHLPEPFVEVHPDDALR YGLMPDGFAKVTTDHGQCILKVVVSERQQRGMLFAPIHWNATNSSHGRVGAMVSSHTDPFSGQPEAKATPASISPYEYVF RGFILSRTELTLPSNLWWVRATIPGGFGYLFADNGDLMRWPGWFKARATDDIADYMDVGGGVYRAASFDGDRIATSVFVG PGHDAGDWNVVKTMFAADHLDDEQRRMLLSGRAPDGTGGGPIVCACFGVGRDTICDAIAAGARTPAEIGAKLKAGTNCGS CIPEMKRLIAQVPAQAEASVRVAS
Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]
COG id: COG0243
COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]
Homologues:
Organism=Escherichia coli, GI3868721, Length=719, Percent_Identity=26.1474269819193, Blast_Score=259, Evalue=8e-70, Organism=Escherichia coli, GI1788534, Length=797, Percent_Identity=27.1016311166876, Blast_Score=257, Evalue=2e-69, Organism=Escherichia coli, GI3868720, Length=620, Percent_Identity=26.9354838709677, Blast_Score=171, Evalue=2e-43, Organism=Escherichia coli, GI3868719, Length=620, Percent_Identity=23.5483870967742, Blast_Score=152, Evalue=7e-38, Organism=Escherichia coli, GI1787778, Length=446, Percent_Identity=25.7847533632287, Blast_Score=119, Evalue=8e-28, Organism=Escherichia coli, GI1787870, Length=491, Percent_Identity=25.8655804480652, Blast_Score=100, Evalue=4e-22, Organism=Escherichia coli, GI171474008, Length=500, Percent_Identity=24.8, Blast_Score=86, Evalue=1e-17, Organism=Escherichia coli, GI87081797, Length=226, Percent_Identity=25.2212389380531, Blast_Score=73, Evalue=1e-13,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR009010 - InterPro: IPR007419 - InterPro: IPR006657 - InterPro: IPR006656 - InterPro: IPR006963 - InterPro: IPR006655 [H]
Pfam domain/function: PF04324 Fer2_BFD; PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]
EC number: =1.7.99.4 [H]
Molecular weight: Translated: 97193; Mature: 97193
Theoretical pI: Translated: 7.61; Mature: 7.61
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.5 %Cys (Translated Protein) 2.5 %Met (Translated Protein) 4.1 %Cys+Met (Translated Protein) 1.5 %Cys (Mature Protein) 2.5 %Met (Mature Protein) 4.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MREGARTMTSVDPKLRTVRTACPYCGVGCGVLATPDGSGGAAISGDPVHPANLGRLCSKG CCCCCCHHHHCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHCC SALGETLGLENRLLYPMVRCKHGMERVAWSDALDHVATRLRHIVARDGEDAVAFYLSGQM CHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCE LTEDYYVANKLMKGFLGSANVDTNSRLCMASSVAGHRRAFGADTVPGTYEDLDQADLLVF ECHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHCCHHHHHCCCCCCCCCHHCCCCCCEEEE VGSNAAWCHPVLYQRMLANKQSRGAKIVVIDPRRTDTASEADLFLGLKPGTDAALFSGLL ECCCCCHHHHHHHHHHHHCHHCCCCEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHH VHLADSGALDRDYIARHTSGFDDTLARARHLAGSAGATALATGLPEADVAAFFKLFRDTP HHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHCCC KVVTLYSQGVNQSAQGTDKVNAIVNCHLATARIGKPGMGPFSLTGQPNAMGGREVGGLAN CEEEEECCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCCCEEECCCCCCCCCHHHHHHHH QLAAHMSFTPPDIDRVRRFWKASDIATHEGLKAVQMFEAIARGQIKALWVIGTNPAVSLP HHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCC DADLVRSALKKLELFVISENVRSNDTVDVGAHVLLPAQAWGEKSGTVTNSERRISRQRAF CHHHHHHHHHHEEEEEEECCCCCCCCEECCCEEEEEHHHCCCCCCCCCCHHHHHHHHHCC MPPVGEAKPDWWILSEVGKRLGHAAAFNYTSAADVFREHAALSAFENDGSRDFDIGGLAT CCCCCCCCCCEEHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE LSDDEYDTMAPVQWPVRAGKPPSPRFFADGAFYTNDRKARFVAPEVPALRSETTAARPLR CCCCCCCCCCCCCCCCCCCCCCCCCEEECCCEEECCCCEEEECCCCCCCCCCCCCCCCEE LNTGRIRDQWHTMTRTGLSPRLGAHLPEPFVEVHPDDALRYGLMPDGFAKVTTDHGQCIL CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHEECCCCCCCEEEECCCHHHHH KVVVSERQQRGMLFAPIHWNATNSSHGRVGAMVSSHTDPFSGQPEAKATPASISPYEYVF HHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHH RGFILSRTELTLPSNLWWVRATIPGGFGYLFADNGDLMRWPGWFKARATDDIADYMDVGG HHHHHCCCEECCCCCEEEEEEECCCCEEEEEECCCCEEECCCCEECCCCHHHHHHHHCCC GVYRAASFDGDRIATSVFVGPGHDAGDWNVVKTMFAADHLDDEQRRMLLSGRAPDGTGGG CEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCC PIVCACFGVGRDTICDAIAAGARTPAEIGAKLKAGTNCGSCIPEMKRLIAQVPAQAEASV CEEEEEECCCHHHHHHHHHHCCCCHHHHCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCE RVAS EECC >Mature Secondary Structure MREGARTMTSVDPKLRTVRTACPYCGVGCGVLATPDGSGGAAISGDPVHPANLGRLCSKG CCCCCCHHHHCCHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHCC SALGETLGLENRLLYPMVRCKHGMERVAWSDALDHVATRLRHIVARDGEDAVAFYLSGQM CHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCE LTEDYYVANKLMKGFLGSANVDTNSRLCMASSVAGHRRAFGADTVPGTYEDLDQADLLVF ECHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHCCHHHHHCCCCCCCCCHHCCCCCCEEEE VGSNAAWCHPVLYQRMLANKQSRGAKIVVIDPRRTDTASEADLFLGLKPGTDAALFSGLL ECCCCCHHHHHHHHHHHHCHHCCCCEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHH VHLADSGALDRDYIARHTSGFDDTLARARHLAGSAGATALATGLPEADVAAFFKLFRDTP HHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHCCC KVVTLYSQGVNQSAQGTDKVNAIVNCHLATARIGKPGMGPFSLTGQPNAMGGREVGGLAN CEEEEECCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCCCEEECCCCCCCCCHHHHHHHH QLAAHMSFTPPDIDRVRRFWKASDIATHEGLKAVQMFEAIARGQIKALWVIGTNPAVSLP HHHHHCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCC DADLVRSALKKLELFVISENVRSNDTVDVGAHVLLPAQAWGEKSGTVTNSERRISRQRAF CHHHHHHHHHHEEEEEEECCCCCCCCEECCCEEEEEHHHCCCCCCCCCCHHHHHHHHHCC MPPVGEAKPDWWILSEVGKRLGHAAAFNYTSAADVFREHAALSAFENDGSRDFDIGGLAT CCCCCCCCCCEEHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE LSDDEYDTMAPVQWPVRAGKPPSPRFFADGAFYTNDRKARFVAPEVPALRSETTAARPLR CCCCCCCCCCCCCCCCCCCCCCCCCEEECCCEEECCCCEEEECCCCCCCCCCCCCCCCEE LNTGRIRDQWHTMTRTGLSPRLGAHLPEPFVEVHPDDALRYGLMPDGFAKVTTDHGQCIL CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHEECCCCCCCEEEECCCHHHHH KVVVSERQQRGMLFAPIHWNATNSSHGRVGAMVSSHTDPFSGQPEAKATPASISPYEYVF HHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHH RGFILSRTELTLPSNLWWVRATIPGGFGYLFADNGDLMRWPGWFKARATDDIADYMDVGG HHHHHCCCEECCCCCEEEEEEECCCCEEEEEECCCCEEECCCCEECCCCHHHHHHHHCCC GVYRAASFDGDRIATSVFVGPGHDAGDWNVVKTMFAADHLDDEQRRMLLSGRAPDGTGGG CEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCC PIVCACFGVGRDTICDAIAAGARTPAEIGAKLKAGTNCGSCIPEMKRLIAQVPAQAEASV CEEEEEECCCHHHHHHHHHHCCCCHHHHCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCE RVAS EECC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8468296 [H]