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The map label for this gene is bioB

Identifier: 146339438

GI number: 146339438

Start: 2581419

End: 2582423

Strand: Direct

Name: bioB

Synonym: BRADO2424

Alternate gene names: 146339438

Gene position: 2581419-2582423 (Clockwise)

Preceding gene: 146339432

Following gene: 146339439

Centisome position: 34.62

GC content: 65.87

Gene sequence:

>1005_bases
GTGCAGGGCGCCCCGCTTCGCCATGACTGGACGCGCGAGGAGGCCGAGGTGCTCTACGCACTGCCGTTCCCCGACCTGAT
GTTTCTCGCTCAGGCCACGCATCGCCGTCACTTCGATCCGACGCGGCTGGAGACCGCGAGTCTGCTCAGCATCAAGACGG
GTGGCTGTCCGGAGGATTGCGGCTACTGCTCGCAGAGCGCGCACTACGACACCGGTCTCAAGGCGACGAAGCTGATGGAC
CAGGACGCGGTCGTCGCGACGGCGCGGCGTGCCAAAGAGGCCGGCGCCGATCGGTTCTGCATGGCCGCCGCCTGGCGCAA
TCCGAAAGACCGTGATCTCGACCAGGTCTGCGACATGGTCAGTGCGGTGAAGGATCTTGGGATGGAGACCTGCGTCACGC
TGGGGATGCTGACGCCGCCACAGGCGGCGCGGCTCAAGCAGGCCGGGCTCGACTACTACAACCACAATGTCGATACCTCG
CCCGAGTTCTACGATCGCATCATCACCACGCGCACCATGCAGGATCGCATCGATACGCTGGCGAATGTCCGCGAGGCCGG
CATCAAGGTGTGCTGCGGCGGCATCATCGGCATGGGCGAGGAGGTCGACGACCGGCTCGGCATGCTGGTGCTGCTGGCCA
ATCTCGATGCGCATCCGGACAGCGTGCCGATCAACATGTGGAACGAGGTCAAGGGCGTGCCTGTCAACGACACGGCGGAG
CGGCCTGACGGAATCGCGCTGGCGCGGCTGATCGCGGTCGCGCGCATCATGATGCCCCGCAGCGTGGTGCGGCTGTCGGC
CGGACGGCAATATATGAGCGACGAGCTGCAGAGCCTGTGCCTGCTCGCCGGCGCCAACTCGATCTTCATCGGCGACGTGC
TGCTGACGACAGCCAATCCGCAGGCGGAGCGCGATGCCGATCTGCTCACGCGGCTCGGCATGACATCGTCATTGTCGGCG
CGCTCGGTTGCAGCGGTCGACGAGAACGTGTCCGCGAACGCCTGA

Upstream 100 bases:

>100_bases
GTCCAGTGATAGCGAATTTTCATAGATAATTTCTATGATAATCTATAACTTTGGGGGTGGTATGGATCGTGTGAGCAGCC
TGTCGTCGTCGGATCTGACA

Downstream 100 bases:

>100_bases
TCCTTCAAATGGCACCGTCACGGCCGGGCTTGCCTCCTGCGGCGCATCCTTTCCGCGCTGCGCAATTTGCGCTCCGAAAA
TCCCCGCATTAGCATCGCAT

Product: biotin synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MQGAPLRHDWTREEAEVLYALPFPDLMFLAQATHRRHFDPTRLETASLLSIKTGGCPEDCGYCSQSAHYDTGLKATKLMD
QDAVVATARRAKEAGADRFCMAAAWRNPKDRDLDQVCDMVSAVKDLGMETCVTLGMLTPPQAARLKQAGLDYYNHNVDTS
PEFYDRIITTRTMQDRIDTLANVREAGIKVCCGGIIGMGEEVDDRLGMLVLLANLDAHPDSVPINMWNEVKGVPVNDTAE
RPDGIALARLIAVARIMMPRSVVRLSAGRQYMSDELQSLCLLAGANSIFIGDVLLTTANPQAERDADLLTRLGMTSSLSA
RSVAAVDENVSANA

Sequences:

>Translated_334_residues
MQGAPLRHDWTREEAEVLYALPFPDLMFLAQATHRRHFDPTRLETASLLSIKTGGCPEDCGYCSQSAHYDTGLKATKLMD
QDAVVATARRAKEAGADRFCMAAAWRNPKDRDLDQVCDMVSAVKDLGMETCVTLGMLTPPQAARLKQAGLDYYNHNVDTS
PEFYDRIITTRTMQDRIDTLANVREAGIKVCCGGIIGMGEEVDDRLGMLVLLANLDAHPDSVPINMWNEVKGVPVNDTAE
RPDGIALARLIAVARIMMPRSVVRLSAGRQYMSDELQSLCLLAGANSIFIGDVLLTTANPQAERDADLLTRLGMTSSLSA
RSVAAVDENVSANA
>Mature_334_residues
MQGAPLRHDWTREEAEVLYALPFPDLMFLAQATHRRHFDPTRLETASLLSIKTGGCPEDCGYCSQSAHYDTGLKATKLMD
QDAVVATARRAKEAGADRFCMAAAWRNPKDRDLDQVCDMVSAVKDLGMETCVTLGMLTPPQAARLKQAGLDYYNHNVDTS
PEFYDRIITTRTMQDRIDTLANVREAGIKVCCGGIIGMGEEVDDRLGMLVLLANLDAHPDSVPINMWNEVKGVPVNDTAE
RPDGIALARLIAVARIMMPRSVVRLSAGRQYMSDELQSLCLLAGANSIFIGDVLLTTANPQAERDADLLTRLGMTSSLSA
RSVAAVDENVSANA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=314, Percent_Identity=55.7324840764331, Blast_Score=372, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6321725, Length=314, Percent_Identity=45.5414012738853, Blast_Score=308, Evalue=9e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_BRASO (A4YQS3)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001204486.1
- STRING:   A4YQS3
- GeneID:   5118991
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO2424
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   LEICSGG
- ProtClustDB:   CLSK2391360
- BioCyc:   BSP376:BRADO2424-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 36446; Mature: 36446

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGAPLRHDWTREEAEVLYALPFPDLMFLAQATHRRHFDPTRLETASLLSIKTGGCPEDC
CCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHEEEEECCCCCHHH
GYCSQSAHYDTGLKATKLMDQDAVVATARRAKEAGADRFCMAAAWRNPKDRDLDQVCDMV
CCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCCHHHHHHHH
SAVKDLGMETCVTLGMLTPPQAARLKQAGLDYYNHNVDTSPEFYDRIITTRTMQDRIDTL
HHHHHCCHHHHHHHCCCCCCHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
ANVREAGIKVCCGGIIGMGEEVDDRLGMLVLLANLDAHPDSVPINMWNEVKGVPVNDTAE
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCCCCCCCCCHHHCCCCCCCCCCCC
RPDGIALARLIAVARIMMPRSVVRLSAGRQYMSDELQSLCLLAGANSIFIGDVLLTTANP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
QAERDADLLTRLGMTSSLSARSVAAVDENVSANA
CHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQGAPLRHDWTREEAEVLYALPFPDLMFLAQATHRRHFDPTRLETASLLSIKTGGCPEDC
CCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHEEEEECCCCCHHH
GYCSQSAHYDTGLKATKLMDQDAVVATARRAKEAGADRFCMAAAWRNPKDRDLDQVCDMV
CCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCEEHHHHCCCCCCCCHHHHHHHH
SAVKDLGMETCVTLGMLTPPQAARLKQAGLDYYNHNVDTSPEFYDRIITTRTMQDRIDTL
HHHHHCCHHHHHHHCCCCCCHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
ANVREAGIKVCCGGIIGMGEEVDDRLGMLVLLANLDAHPDSVPINMWNEVKGVPVNDTAE
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCCCCCCCCCHHHCCCCCCCCCCCC
RPDGIALARLIAVARIMMPRSVVRLSAGRQYMSDELQSLCLLAGANSIFIGDVLLTTANP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
QAERDADLLTRLGMTSSLSARSVAAVDENVSANA
CHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA