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The map label for this gene is mdtE [H]

Identifier: 146338957

GI number: 146338957

Start: 2024218

End: 2025381

Strand: Direct

Name: mdtE [H]

Synonym: BRADO1901

Alternate gene names: 146338957

Gene position: 2024218-2025381 (Clockwise)

Preceding gene: 146338956

Following gene: 146338958

Centisome position: 27.15

GC content: 67.7

Gene sequence:

>1164_bases
TTGACAATCGTCAGTCGACAATCGACACTCTCTCGCAATCGCAGCGAGGGGTGTCCCATGTTGATCCGCCTGATTTTCGC
GAGCTATTCCAAGCTTTTGACCGGCATCGCGCTGGCGACCATGGCTGCCGCCCTGGCCGGTTGCAACGAAACAGTCGCCC
AAAAGGTCGAGCCGGTCCGGCCGGTTCTGGTCGCATCCGTTCATTACGAGGCCGAGACGCCGGAGCGCAGCTTCGTTGGC
ACGATCCGGCCGCGCATCGAGGCGGACATCGGCTTCCGCGTCGCCGGCAAGGTGGCCAAGCGCCTCGTCGAGGTCGGCCA
GACCATCGAGGTCGGCCAGCCGCTCGCCACCCTGGATGAGGTCGACCTGAAGCTGCAGGCCGAGCAGGCCGAGGCGGAAT
TCCGCGCCGCCACTGGCGTGCTCGCGCAGGCCTCCGCCGCCGAGACCCGCGCCAAGGAATTGCGTGCCAAGGGCTGGACC
ACCGACGCGCAGATGGATCAGGCCAAGGCCTCCGCCGACGAGGCCCGCGCGCGGCTCAATCGCGCCGAGCGCTCGGTCGA
GCTGACGCGCAATTCGTTGTCCTACGCGACGCTGGTGTCGGACGCGCGCGGCGTCGTCACGGCGAGCATGATCGAGCCCG
GCGTCGTCGTCGCCGCCGGCCAGGCCGCCATCCGCGTCGCGCGCTTCGCTGAAAAGGAGGCGGTGGTCGCCATCCCCGAG
ACCTTGCTCGATCGTGCCAAGAACGGCTCAGCGACGGTGTCGCTGTGGTCGGAGGCGGGCAAGACCTACACGGCGCGGCT
GCGCGAGATCGCTCCGGCCGCCGATCCGGCGACGCGCACCTATCTCGCAAAGTTCTCGCTGCCGGATGCGAATGACAGCG
TCTCGCTCGGCATGACCGCGACGCTCACCTTGTCCGATCCGGCGACCATGCGCGTCGCGCGGCTGCCGCTGTCGGCGCTG
TTCAACGACGGCCGCGATCCCTCGCTCTACGTCGTCAACGACAAGGGCGAGGTGACGCTGAAGCCGGTCAAGGTGAAGGC
CTATGACAGCAAGGACGTGCTGATCGTCTCAGGCGTCGACGAGGGCGAGAAGGTGGTCGCCCTCGGCGTGCAGAAGATCG
ACCCCGCGCAGAAGGTGCGCGTGGTCTCCGCGCTGACGTTCTGA

Upstream 100 bases:

>100_bases
GGGGCGGGCGGACTGCCGCGCGCCGAGAGAATCCCTGCTTCTCCCCGACCCCTGCGGCAACTTGTCTTAACGCTCACGAA
GTCATGATCGCTGACGATTA

Downstream 100 bases:

>100_bases
GGGTCTCAAGGTTTGATGTCTTGGGGCTTTAACGGCCTGGCGGACTTTCAACCCTCACCCTGAGGAGCCCGCCGCAGGCG
GTCGTCTCGAAGGGCGAGGC

Product: putative component of multidrug efflux system signal peptide

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MTIVSRQSTLSRNRSEGCPMLIRLIFASYSKLLTGIALATMAAALAGCNETVAQKVEPVRPVLVASVHYEAETPERSFVG
TIRPRIEADIGFRVAGKVAKRLVEVGQTIEVGQPLATLDEVDLKLQAEQAEAEFRAATGVLAQASAAETRAKELRAKGWT
TDAQMDQAKASADEARARLNRAERSVELTRNSLSYATLVSDARGVVTASMIEPGVVVAAGQAAIRVARFAEKEAVVAIPE
TLLDRAKNGSATVSLWSEAGKTYTARLREIAPAADPATRTYLAKFSLPDANDSVSLGMTATLTLSDPATMRVARLPLSAL
FNDGRDPSLYVVNDKGEVTLKPVKVKAYDSKDVLIVSGVDEGEKVVALGVQKIDPAQKVRVVSALTF

Sequences:

>Translated_387_residues
MTIVSRQSTLSRNRSEGCPMLIRLIFASYSKLLTGIALATMAAALAGCNETVAQKVEPVRPVLVASVHYEAETPERSFVG
TIRPRIEADIGFRVAGKVAKRLVEVGQTIEVGQPLATLDEVDLKLQAEQAEAEFRAATGVLAQASAAETRAKELRAKGWT
TDAQMDQAKASADEARARLNRAERSVELTRNSLSYATLVSDARGVVTASMIEPGVVVAAGQAAIRVARFAEKEAVVAIPE
TLLDRAKNGSATVSLWSEAGKTYTARLREIAPAADPATRTYLAKFSLPDANDSVSLGMTATLTLSDPATMRVARLPLSAL
FNDGRDPSLYVVNDKGEVTLKPVKVKAYDSKDVLIVSGVDEGEKVVALGVQKIDPAQKVRVVSALTF
>Mature_386_residues
TIVSRQSTLSRNRSEGCPMLIRLIFASYSKLLTGIALATMAAALAGCNETVAQKVEPVRPVLVASVHYEAETPERSFVGT
IRPRIEADIGFRVAGKVAKRLVEVGQTIEVGQPLATLDEVDLKLQAEQAEAEFRAATGVLAQASAAETRAKELRAKGWTT
DAQMDQAKASADEARARLNRAERSVELTRNSLSYATLVSDARGVVTASMIEPGVVVAAGQAAIRVARFAEKEAVVAIPET
LLDRAKNGSATVSLWSEAGKTYTARLREIAPAADPATRTYLAKFSLPDANDSVSLGMTATLTLSDPATMRVARLPLSALF
NDGRDPSLYVVNDKGEVTLKPVKVKAYDSKDVLIVSGVDEGEKVVALGVQKIDPAQKVRVVSALTF

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=379, Percent_Identity=24.5382585751979, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789665, Length=367, Percent_Identity=23.7057220708447, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081796, Length=305, Percent_Identity=25.2459016393443, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41280; Mature: 41148

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIVSRQSTLSRNRSEGCPMLIRLIFASYSKLLTGIALATMAAALAGCNETVAQKVEPVR
CCEECCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCC
PVLVASVHYEAETPERSFVGTIRPRIEADIGFRVAGKVAKRLVEVGQTIEVGQPLATLDE
CEEEEEEEECCCCCCCHHEECCCCCEECCCCHHHHHHHHHHHHHCCCCEECCCCHHHHHH
VDLKLQAEQAEAEFRAATGVLAQASAAETRAKELRAKGWTTDAQMDQAKASADEARARLN
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
RAERSVELTRNSLSYATLVSDARGVVTASMIEPGVVVAAGQAAIRVARFAEKEAVVAIPE
HHHHHHHHHHCCCHHHHHHHCCCCEEEEECCCCCEEEECCHHHHHHHHHHCCCEEEECCH
TLLDRAKNGSATVSLWSEAGKTYTARLREIAPAADPATRTYLAKFSLPDANDSVSLGMTA
HHHHCCCCCCEEEEEHHHCCCHHHHHHHHHCCCCCCCHHEEEEEECCCCCCCCEEECEEE
TLTLSDPATMRVARLPLSALFNDGRDPSLYVVNDKGEVTLKPVKVKAYDSKDVLIVSGVD
EEEECCCCHHHHHHCCHHHHHCCCCCCEEEEECCCCCEEEEEEEEEEECCCCEEEEECCC
EGEKVVALGVQKIDPAQKVRVVSALTF
CCCEEEEEECHHCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TIVSRQSTLSRNRSEGCPMLIRLIFASYSKLLTGIALATMAAALAGCNETVAQKVEPVR
CEECCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCC
PVLVASVHYEAETPERSFVGTIRPRIEADIGFRVAGKVAKRLVEVGQTIEVGQPLATLDE
CEEEEEEEECCCCCCCHHEECCCCCEECCCCHHHHHHHHHHHHHCCCCEECCCCHHHHHH
VDLKLQAEQAEAEFRAATGVLAQASAAETRAKELRAKGWTTDAQMDQAKASADEARARLN
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
RAERSVELTRNSLSYATLVSDARGVVTASMIEPGVVVAAGQAAIRVARFAEKEAVVAIPE
HHHHHHHHHHCCCHHHHHHHCCCCEEEEECCCCCEEEECCHHHHHHHHHHCCCEEEECCH
TLLDRAKNGSATVSLWSEAGKTYTARLREIAPAADPATRTYLAKFSLPDANDSVSLGMTA
HHHHCCCCCCEEEEEHHHCCCHHHHHHHHHCCCCCCCHHEEEEEECCCCCCCCEEECEEE
TLTLSDPATMRVARLPLSALFNDGRDPSLYVVNDKGEVTLKPVKVKAYDSKDVLIVSGVD
EEEECCCCHHHHHHCCHHHHHCCCCCCEEEEECCCCCEEEEEEEEEEECCCCEEEEECCC
EGEKVVALGVQKIDPAQKVRVVSALTF
CCCEEEEEECHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]