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The map label for this gene is vanB [H]

Identifier: 146338911

GI number: 146338911

Start: 1976012

End: 1976974

Strand: Reverse

Name: vanB [H]

Synonym: BRADO1851

Alternate gene names: 146338911

Gene position: 1976974-1976012 (Counterclockwise)

Preceding gene: 146338913

Following gene: 146338910

Centisome position: 26.51

GC content: 63.66

Gene sequence:

>963_bases
ATGTCCGACAGTCTGCACACCGTTGTCGTGGCCAGGAAGGCCATCGAGGCGCACAACATGGCCTCGTTCGAGCTGGTCCC
GGCGGATGACCAGCCGCTGCCGGGGTTCACGCCTGGCAGCCATATCGACGTCACCCTCCCAAACGGGCTGACGCGGCAAT
ATTCGCTTTTGAACAGCGCCACCGAGCGCGACCGCTACTGCATCGGCGTTTGGAAGGATGCCAACAGCCGCGGCGGCTCC
AAGGCCTTGCATCTCGACGTCAACGAAGGCGACCGGCTGCAGGTCAGCCGCCCCCGCAACCGCTTCAAGATCCCGAAAGA
CATCAAGCGGGCGCTGCTGGTCGCGCGCGGCATCGGCGTGACGCCAATTCTCTCGATCGCCGACACGCTGAAGGCGAAGA
AGATCCCGTTCGAGCTGCACTACGTGTTCGCGCTGATGTCGCCGGATTCGTTCCGCGGCACGATCGAGGCGTCGTCTTTC
GCCGACAACACGACCTACTACAAGGAAGCCACCGAGGACAATCAGCTGTTGAAGGCGGCCGATCTGCTGGCGGACCGTCC
TGACGACACCGAGCTGTTCATCTGCGGCGTCGATTGGTGGATGGATCCGATCATTGCGCTCGCCAAGCAGAAGGGATTCG
CCGAGGAGCGCATCCATGTCGAACGTTTCACCGCGAAGGCCGCCGCCGCGCTGCTCGACAAGGTGTTCGACGTCACCATT
AAGAGCACCGGCGCGACCTTCAAGATCCCGGGCGACAAGACCGTCACGGCGTTCCTCGAGGAGAACGGCGTCAAGATCGC
GACCTCCTGCGAGCAGGGAATGTGCGGCACCTGCAAGACGAAAGTCGTCGACGGCGACATCGATCACCGCGACAAACGGC
TCTCCGCCGCGCAGCGCGCCGAGGGCTATTTCCTGCCCTGCGTCTCGCGCGCCAAGGGCGACCGGCTGGTGCTGGATCTC
TAG

Upstream 100 bases:

>100_bases
CGCACACAAGGGGAAAAACATTGCGGTGCGCGTGCTTCACCGTTTGGCCTCTCCCTTGCATCGTACCTTCGTCAGCCGGT
TCGCAAGATGGAGTTGTCAG

Downstream 100 bases:

>100_bases
TACCGTGACCACCGAGATCAATCTGTGGCAGACGGCCTGGATCATCGCCGAGCAGCATGGCCGCGACGGGCCGGCCTTTG
CGGACCGCATGGCTGACAGC

Product: vanillate O-demethylase oxidoreductase

Products: 3,4-dihydroxybenzoate; NAD+; H2O; formaldehyde

Alternate protein names: Vanillate degradation ferredoxin-like protein [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSDSLHTVVVARKAIEAHNMASFELVPADDQPLPGFTPGSHIDVTLPNGLTRQYSLLNSATERDRYCIGVWKDANSRGGS
KALHLDVNEGDRLQVSRPRNRFKIPKDIKRALLVARGIGVTPILSIADTLKAKKIPFELHYVFALMSPDSFRGTIEASSF
ADNTTYYKEATEDNQLLKAADLLADRPDDTELFICGVDWWMDPIIALAKQKGFAEERIHVERFTAKAAAALLDKVFDVTI
KSTGATFKIPGDKTVTAFLEENGVKIATSCEQGMCGTCKTKVVDGDIDHRDKRLSAAQRAEGYFLPCVSRAKGDRLVLDL

Sequences:

>Translated_320_residues
MSDSLHTVVVARKAIEAHNMASFELVPADDQPLPGFTPGSHIDVTLPNGLTRQYSLLNSATERDRYCIGVWKDANSRGGS
KALHLDVNEGDRLQVSRPRNRFKIPKDIKRALLVARGIGVTPILSIADTLKAKKIPFELHYVFALMSPDSFRGTIEASSF
ADNTTYYKEATEDNQLLKAADLLADRPDDTELFICGVDWWMDPIIALAKQKGFAEERIHVERFTAKAAAALLDKVFDVTI
KSTGATFKIPGDKTVTAFLEENGVKIATSCEQGMCGTCKTKVVDGDIDHRDKRLSAAQRAEGYFLPCVSRAKGDRLVLDL
>Mature_319_residues
SDSLHTVVVARKAIEAHNMASFELVPADDQPLPGFTPGSHIDVTLPNGLTRQYSLLNSATERDRYCIGVWKDANSRGGSK
ALHLDVNEGDRLQVSRPRNRFKIPKDIKRALLVARGIGVTPILSIADTLKAKKIPFELHYVFALMSPDSFRGTIEASSFA
DNTTYYKEATEDNQLLKAADLLADRPDDTELFICGVDWWMDPIIALAKQKGFAEERIHVERFTAKAAAALLDKVFDVTIK
STGATFKIPGDKTVTAFLEENGVKIATSCEQGMCGTCKTKVVDGDIDHRDKRLSAAQRAEGYFLPCVSRAKGDRLVLDL

Specific function: Unknown

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788104, Length=329, Percent_Identity=34.6504559270517, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1787098, Length=251, Percent_Identity=31.0756972111554, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787658, Length=352, Percent_Identity=23.8636363636364, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.14.13.82

Molecular weight: Translated: 35257; Mature: 35125

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSLHTVVVARKAIEAHNMASFELVPADDQPLPGFTPGSHIDVTLPNGLTRQYSLLNSA
CCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHH
TERDRYCIGVWKDANSRGGSKALHLDVNEGDRLQVSRPRNRFKIPKDIKRALLVARGIGV
HCCCCEEEEEEECCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCC
TPILSIADTLKAKKIPFELHYVFALMSPDSFRGTIEASSFADNTTYYKEATEDNQLLKAA
CHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCEEEECCCCCCCEEEECCCCCHHHHHHH
DLLADRPDDTELFICGVDWWMDPIIALAKQKGFAEERIHVERFTAKAAAALLDKVFDVTI
HHHCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEE
KSTGATFKIPGDKTVTAFLEENGVKIATSCEQGMCGTCKTKVVDGDIDHRDKRLSAAQRA
ECCCCEEEECCCCEEEEEEECCCEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHC
EGYFLPCVSRAKGDRLVLDL
CCEEEECCCCCCCCEEEEEC
>Mature Secondary Structure 
SDSLHTVVVARKAIEAHNMASFELVPADDQPLPGFTPGSHIDVTLPNGLTRQYSLLNSA
CCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHH
TERDRYCIGVWKDANSRGGSKALHLDVNEGDRLQVSRPRNRFKIPKDIKRALLVARGIGV
HCCCCEEEEEEECCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCC
TPILSIADTLKAKKIPFELHYVFALMSPDSFRGTIEASSFADNTTYYKEATEDNQLLKAA
CHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCEEEECCCCCCCEEEECCCCCHHHHHHH
DLLADRPDDTELFICGVDWWMDPIIALAKQKGFAEERIHVERFTAKAAAALLDKVFDVTI
HHHCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEE
KSTGATFKIPGDKTVTAFLEENGVKIATSCEQGMCGTCKTKVVDGDIDHRDKRLSAAQRA
ECCCCEEEECCCCEEEEEEECCCEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHC
EGYFLPCVSRAKGDRLVLDL
CCEEEECCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: vanillate; O2; NADH; H+

Specific reaction: vanillate + O2 + NADH + H+ = 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9098058 [H]