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The map label for this gene is ligB [H]

Identifier: 146338913

GI number: 146338913

Start: 1978450

End: 1979730

Strand: Reverse

Name: ligB [H]

Synonym: BRADO1853

Alternate gene names: 146338913

Gene position: 1979730-1978450 (Counterclockwise)

Preceding gene: 146338915

Following gene: 146338911

Centisome position: 26.55

GC content: 64.25

Gene sequence:

>1281_bases
ATGGCCCGGATCATTGGCGGTATCGGCGCGTCGCACTCGCCTACCATCGGCTTCGCGCTGGACATGAAGAAGCAGAACGA
TCCGGCCTGGAAGCCGATCTTCGACGGCTTTGCCGCCGTGCGCGACTGGGTGAGACGCAAGGACATTGACGTCCTGTTCA
TGATCTACAACGATCACATCACCTCGTTCTTCTTCGACCACTATTCCGCCTTCGCGCTCGGCATCGACGACCACTATGTC
GCGGCCGACGAAGGCGGCGGACCGCGCGACGTGCCGCCGGCCAGGGGCCATCTCGGCCTGTCGCAGCACATCGCGATGGC
CATGGTGGCCGACGAGTTCGACATGTCCTTCTTCCAGGGCAAGCCGGTCGATCACGGCCTGTTGTCGCCGCTGTCGATGA
TGGGCGATGCGGACGGACCGTGGCCTGGTACCGTCGTCCCGCTGCAGGTCGGCGTGCTGCAACTGCCGATCCCGAATGCC
AAGCGGATGTGGAAGCTCGGCAAGACCTTGCGCAAGGCGATCGAGAGCTATCCGGAGGATCTGAAGGTTGCGATCTTCGC
CACCGGCGGACTGTCGCACCAGGTTCATGGCGAGCGCGCCGGCTTCCTGAACGAGGATTGGGACAACGAGTTTCTCGACC
TCTTGGAGAAGAATCCGGAGCAGCTGACCGCGATGCGAATCGCCGACTACGCGCGCAAGGGCGGCATGGAAGGCGCCGAG
GTGATCATGTGGCTGATCATGCGCGGGGCCCTATCGGATGAGGTGAGCCTGGTGCACCGGCAGACCTATGCGCCGTCGGT
GACCAACATCGCGACGCTGGTGTTCGAGGATCTGGGCGCGGCGCCGGACGCGCAGGACGTCGCAGCCTACCGCGCCCATA
TCGGTCACGAACTCGTGGGCGCCGAGACGCTGGCCGGCACTTACCCGTTCACCCAGGACCGCAGCCAGGCGAATTTCCGC
ATCAACGACTTCCTGCACCAGCTCGTCGTCCCGGCGCATCGCAGGCAATTCCTTGAGGATTTCGACGCGCTGGCGGATGC
CTACAAGCTCACCGACGAGGAGTGTGACCTGATCAAGAACCGGCGCTGGATCGAGATGATCCGGCGCGGCGTCAGCTTCT
TCGTGCTGGAGAAGATGGCCGCCGTGATCGGCGTCTCCAACCCGGAGGTCTATGCCGCCTTCCGCGGCGAGACGCTGGAA
GCCTTCCTGAAGACCCGCAAGGTGCCGATGCGCTACTCCGTCGCGGGCGGCGCCAAGGCGGAGACGATCGACGCGCATTG
A

Upstream 100 bases:

>100_bases
TACACGCTAGCATTATTGTCTCGCCAGCGGAACTTCGTAATAGTCCGGCCCCGTGCGGCTGCGCGTCTCGGTCGGAAGCC
GCGGAGTTCGCGGAGGACAC

Downstream 100 bases:

>100_bases
CCGCCGCACGCGCAAGACCTTCACGCGCATGACCCGCGCCGCCGGCGGCGACGCGGGTCATGTCTCCGGCAAGTTGATCC
CGCGCGCCGTCGCCCAGCGC

Product: protocatechuate 4,5-dioxygenase

Products: NA

Alternate protein names: 4,5-PCD [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MARIIGGIGASHSPTIGFALDMKKQNDPAWKPIFDGFAAVRDWVRRKDIDVLFMIYNDHITSFFFDHYSAFALGIDDHYV
AADEGGGPRDVPPARGHLGLSQHIAMAMVADEFDMSFFQGKPVDHGLLSPLSMMGDADGPWPGTVVPLQVGVLQLPIPNA
KRMWKLGKTLRKAIESYPEDLKVAIFATGGLSHQVHGERAGFLNEDWDNEFLDLLEKNPEQLTAMRIADYARKGGMEGAE
VIMWLIMRGALSDEVSLVHRQTYAPSVTNIATLVFEDLGAAPDAQDVAAYRAHIGHELVGAETLAGTYPFTQDRSQANFR
INDFLHQLVVPAHRRQFLEDFDALADAYKLTDEECDLIKNRRWIEMIRRGVSFFVLEKMAAVIGVSNPEVYAAFRGETLE
AFLKTRKVPMRYSVAGGAKAETIDAH

Sequences:

>Translated_426_residues
MARIIGGIGASHSPTIGFALDMKKQNDPAWKPIFDGFAAVRDWVRRKDIDVLFMIYNDHITSFFFDHYSAFALGIDDHYV
AADEGGGPRDVPPARGHLGLSQHIAMAMVADEFDMSFFQGKPVDHGLLSPLSMMGDADGPWPGTVVPLQVGVLQLPIPNA
KRMWKLGKTLRKAIESYPEDLKVAIFATGGLSHQVHGERAGFLNEDWDNEFLDLLEKNPEQLTAMRIADYARKGGMEGAE
VIMWLIMRGALSDEVSLVHRQTYAPSVTNIATLVFEDLGAAPDAQDVAAYRAHIGHELVGAETLAGTYPFTQDRSQANFR
INDFLHQLVVPAHRRQFLEDFDALADAYKLTDEECDLIKNRRWIEMIRRGVSFFVLEKMAAVIGVSNPEVYAAFRGETLE
AFLKTRKVPMRYSVAGGAKAETIDAH
>Mature_425_residues
ARIIGGIGASHSPTIGFALDMKKQNDPAWKPIFDGFAAVRDWVRRKDIDVLFMIYNDHITSFFFDHYSAFALGIDDHYVA
ADEGGGPRDVPPARGHLGLSQHIAMAMVADEFDMSFFQGKPVDHGLLSPLSMMGDADGPWPGTVVPLQVGVLQLPIPNAK
RMWKLGKTLRKAIESYPEDLKVAIFATGGLSHQVHGERAGFLNEDWDNEFLDLLEKNPEQLTAMRIADYARKGGMEGAEV
IMWLIMRGALSDEVSLVHRQTYAPSVTNIATLVFEDLGAAPDAQDVAAYRAHIGHELVGAETLAGTYPFTQDRSQANFRI
NDFLHQLVVPAHRRQFLEDFDALADAYKLTDEECDLIKNRRWIEMIRRGVSFFVLEKMAAVIGVSNPEVYAAFRGETLEA
FLKTRKVPMRYSVAGGAKAETIDAH

Specific function: Responsible for the aromatic ring fission of protocatechuate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: =1.13.11.8 [H]

Molecular weight: Translated: 47335; Mature: 47203

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIIGGIGASHSPTIGFALDMKKQNDPAWKPIFDGFAAVRDWVRRKDIDVLFMIYNDHI
CCCEECCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCHH
TSFFFDHYSAFALGIDDHYVAADEGGGPRDVPPARGHLGLSQHIAMAMVADEFDMSFFQG
HHHHHHHHHEEEECCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
KPVDHGLLSPLSMMGDADGPWPGTVVPLQVGVLQLPIPNAKRMWKLGKTLRKAIESYPED
CCCCCHHHHHHHHHCCCCCCCCCCEEEEEECEEEECCCCHHHHHHHHHHHHHHHHHCCCC
LKVAIFATGGLSHQVHGERAGFLNEDWDNEFLDLLEKNPEQLTAMRIADYARKGGMEGAE
EEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHH
VIMWLIMRGALSDEVSLVHRQTYAPSVTNIATLVFEDLGAAPDAQDVAAYRAHIGHELVG
HHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHC
AETLAGTYPFTQDRSQANFRINDFLHQLVVPAHRRQFLEDFDALADAYKLTDEECDLIKN
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHC
RRWIEMIRRGVSFFVLEKMAAVIGVSNPEVYAAFRGETLEAFLKTRKVPMRYSVAGGAKA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHCCCCCEEEECCCCCC
ETIDAH
CCCCCC
>Mature Secondary Structure 
ARIIGGIGASHSPTIGFALDMKKQNDPAWKPIFDGFAAVRDWVRRKDIDVLFMIYNDHI
CCEECCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCHH
TSFFFDHYSAFALGIDDHYVAADEGGGPRDVPPARGHLGLSQHIAMAMVADEFDMSFFQG
HHHHHHHHHEEEECCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
KPVDHGLLSPLSMMGDADGPWPGTVVPLQVGVLQLPIPNAKRMWKLGKTLRKAIESYPED
CCCCCHHHHHHHHHCCCCCCCCCCEEEEEECEEEECCCCHHHHHHHHHHHHHHHHHCCCC
LKVAIFATGGLSHQVHGERAGFLNEDWDNEFLDLLEKNPEQLTAMRIADYARKGGMEGAE
EEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHH
VIMWLIMRGALSDEVSLVHRQTYAPSVTNIATLVFEDLGAAPDAQDVAAYRAHIGHELVG
HHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHC
AETLAGTYPFTQDRSQANFRINDFLHQLVVPAHRRQFLEDFDALADAYKLTDEECDLIKN
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHC
RRWIEMIRRGVSFFVLEKMAAVIGVSNPEVYAAFRGETLEAFLKTRKVPMRYSVAGGAKA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHCCCCCEEEECCCCCC
ETIDAH
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2185230 [H]