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The map label for this gene is acyI [H]

Identifier: 146338606

GI number: 146338606

Start: 1638467

End: 1640062

Strand: Reverse

Name: acyI [H]

Synonym: BRADO1534

Alternate gene names: 146338606

Gene position: 1640062-1638467 (Counterclockwise)

Preceding gene: 146338607

Following gene: 146338604

Centisome position: 21.99

GC content: 69.74

Gene sequence:

>1596_bases
ATGCCGTACCAGTTTTCCAATCGCCAGCAGATCCGCAAGCCGGCGGTGACCAGCAAGGGTGGCATCGTCGCGGCGCAGTC
GCGGCGCGCGGCCGAGATCGGTGCCGAGGTGCTGGCCGCCGGCGGCGACTGCATCGATGCCGTCGTGGCGACCGGCTTCG
CGCTCGGCGTCGTCGAGCCGTGGATGAGCGGGCTGGGCGGCGGCGGCGCCATGGTGCTGTACCGCGCCAAGGATAACAAG
GTCGAGGTGATCGACTACGGCATGCGCGCGCCGGACAGTTTGCGCGTCGAGGACTACCCGCTGACCAATGACGGCAGCGT
GTCCCCCGACATCTTCCCGTGGCCCCGCGTCAAGGACGACCGCAACCTGCACGGCCCTGGCGCGATCGCGGTGCCCGGCG
TGGTGGCCGGCATGGACATGGCGTACCGCCGCTACGCCAGGCTGCCTTGGCAGGACCTCGTCGCGCCGAGCGTCGCGCTG
GCCGCCGAGGGGCTGCTGGTGGACTGGTGGACCACCGTGATGATCTCAAGCTGCGCCGCCGACCTGCGCCGTTATGCGGG
CAGCGCCGCGGCCTATCTGAAGGACGGCCTGCCGCCGAACCCGCAATGGGGCGCCAAGGCGATCACGCGGCTGCCGCAGG
AGCAGCTCAAGGCGACCTTGGCGCATCTCGCCAGCACCGGCGCGCGCGACTTCTACCAGGGCGACATCGCGCGGTCGCTG
ACCCGCGACATCCAGGCCGCCGGCGGCGCACTCTCGGTCGAGGATCTCGCCGCGTTCGCCGCGCAGGTCCGTGATCCCTT
GCGCATTCCCTATCGCGGCGGCGCGGTGTTCGCGACGCCGGAGCTCACCGCCGGACCAACCTTGGCGCATGCGCTTCGGC
TGATGCAGACGAGCATCGAGCCTGCAGGCAAGGCTCCGGACGCAGGCGCCTTCATCGCCTATGCGGAAGCGATGCAGTCG
GCCTATCGCGAGCGGCTCAACGACATGGGCGATGCGCCCGGCAAGCGTGCGCTCGGCGCCGAATATCTCGCGCCGGCCTG
CACCACGCATTATTCGGTCGTCGACCGCGACGGCAACATGGCCGCGGTGACGCAGACCCTGCTGTCCGGCTTCGGCTCGA
AGTTCCAGGCGCCGCAGAGCGGCATCATGATGAACAACGGCATCATGTGGTTCGACCCGACGCCGGGCACGACCAACTCG
CTGGCGCCGGGCAAGCGCTGCCTCACCAACTACACGCCGGTCGTGGCCGAAACCGCCGACGGCCGTCGCCTCGCACTGGG
CGCCTCTGGCGGCCGCCGCATTCTCCCGGCCGTGGCGCAGATGCTGTCCTTCGTGATGGACTATCGTATGGACTTGGACG
CTGCGATCCATCAGCCCCGCATCGACGCAAGCGAGGGCGACGTGGTCGGCGCCGACACGCGGCTGCCGGCGGAGGTGATG
AGTGCGCTCACGGCGAAGTTCGAAACCGTGCCGGCACCGGTGCAGACCATGCCGATGAAGTTCGCCTGCCCCAGCATCGT
GCTGCGCGACGGCGTAACGAACAGCGGCGCGACGGAGATCGCGCATGCCTGGGGCGATGCGGTGGCGGAGGGGTGA

Upstream 100 bases:

>100_bases
TTCGATCTGAGCTGAGGAGGCAGGCGCCGTTGCCGATTGTGCGGCGGCGCCGGCCGGTGTCAATTTGCCCGACGAATGAG
GTGGATCGAAGGGGAATCCC

Downstream 100 bases:

>100_bases
TTTCCGCGCGACCTGCGGCCATTACGCCGGCAGGCCCGCTCCCGCCAGCCTGTCGGCGAGGAACGTCGTCAACTCCCCCA
CCGTCCGGGAGTTGGTCCGC

Product: putative gamma-glutamyltranspeptidase

Products: NA

Alternate protein names: Cephalosporin acylase; GL-7ACA acylase; Gamma-glutamyltranspeptidase; GGT [H]

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MPYQFSNRQQIRKPAVTSKGGIVAAQSRRAAEIGAEVLAAGGDCIDAVVATGFALGVVEPWMSGLGGGGAMVLYRAKDNK
VEVIDYGMRAPDSLRVEDYPLTNDGSVSPDIFPWPRVKDDRNLHGPGAIAVPGVVAGMDMAYRRYARLPWQDLVAPSVAL
AAEGLLVDWWTTVMISSCAADLRRYAGSAAAYLKDGLPPNPQWGAKAITRLPQEQLKATLAHLASTGARDFYQGDIARSL
TRDIQAAGGALSVEDLAAFAAQVRDPLRIPYRGGAVFATPELTAGPTLAHALRLMQTSIEPAGKAPDAGAFIAYAEAMQS
AYRERLNDMGDAPGKRALGAEYLAPACTTHYSVVDRDGNMAAVTQTLLSGFGSKFQAPQSGIMMNNGIMWFDPTPGTTNS
LAPGKRCLTNYTPVVAETADGRRLALGASGGRRILPAVAQMLSFVMDYRMDLDAAIHQPRIDASEGDVVGADTRLPAEVM
SALTAKFETVPAPVQTMPMKFACPSIVLRDGVTNSGATEIAHAWGDAVAEG

Sequences:

>Translated_531_residues
MPYQFSNRQQIRKPAVTSKGGIVAAQSRRAAEIGAEVLAAGGDCIDAVVATGFALGVVEPWMSGLGGGGAMVLYRAKDNK
VEVIDYGMRAPDSLRVEDYPLTNDGSVSPDIFPWPRVKDDRNLHGPGAIAVPGVVAGMDMAYRRYARLPWQDLVAPSVAL
AAEGLLVDWWTTVMISSCAADLRRYAGSAAAYLKDGLPPNPQWGAKAITRLPQEQLKATLAHLASTGARDFYQGDIARSL
TRDIQAAGGALSVEDLAAFAAQVRDPLRIPYRGGAVFATPELTAGPTLAHALRLMQTSIEPAGKAPDAGAFIAYAEAMQS
AYRERLNDMGDAPGKRALGAEYLAPACTTHYSVVDRDGNMAAVTQTLLSGFGSKFQAPQSGIMMNNGIMWFDPTPGTTNS
LAPGKRCLTNYTPVVAETADGRRLALGASGGRRILPAVAQMLSFVMDYRMDLDAAIHQPRIDASEGDVVGADTRLPAEVM
SALTAKFETVPAPVQTMPMKFACPSIVLRDGVTNSGATEIAHAWGDAVAEG
>Mature_530_residues
PYQFSNRQQIRKPAVTSKGGIVAAQSRRAAEIGAEVLAAGGDCIDAVVATGFALGVVEPWMSGLGGGGAMVLYRAKDNKV
EVIDYGMRAPDSLRVEDYPLTNDGSVSPDIFPWPRVKDDRNLHGPGAIAVPGVVAGMDMAYRRYARLPWQDLVAPSVALA
AEGLLVDWWTTVMISSCAADLRRYAGSAAAYLKDGLPPNPQWGAKAITRLPQEQLKATLAHLASTGARDFYQGDIARSLT
RDIQAAGGALSVEDLAAFAAQVRDPLRIPYRGGAVFATPELTAGPTLAHALRLMQTSIEPAGKAPDAGAFIAYAEAMQSA
YRERLNDMGDAPGKRALGAEYLAPACTTHYSVVDRDGNMAAVTQTLLSGFGSKFQAPQSGIMMNNGIMWFDPTPGTTNSL
APGKRCLTNYTPVVAETADGRRLALGASGGRRILPAVAQMLSFVMDYRMDLDAAIHQPRIDASEGDVVGADTRLPAEVMS
ALTAKFETVPAPVQTMPMKFACPSIVLRDGVTNSGATEIAHAWGDAVAEG

Specific function: Besides the cephalosporin acylase I activity which converts GL-7ACA into 7-ACA; this enzyme displays some gamma glutamyltranspeptidase activity [H]

COG id: COG0405

COG function: function code E; Gamma-glutamyltransferase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI73915092, Length=535, Percent_Identity=25.7943925233645, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI73915090, Length=535, Percent_Identity=25.7943925233645, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI73915096, Length=535, Percent_Identity=25.7943925233645, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI73915094, Length=535, Percent_Identity=25.7943925233645, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI113429324, Length=536, Percent_Identity=25.3731343283582, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI113429326, Length=540, Percent_Identity=25.3703703703704, Blast_Score=136, Evalue=6e-32,
Organism=Homo sapiens, GI153266885, Length=517, Percent_Identity=25.9187620889749, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI153266901, Length=518, Percent_Identity=26.6409266409266, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI109148539, Length=521, Percent_Identity=26.679462571977, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI153266907, Length=515, Percent_Identity=23.8834951456311, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI30581157, Length=152, Percent_Identity=30.9210526315789, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI30581155, Length=152, Percent_Identity=30.9210526315789, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1789856, Length=497, Percent_Identity=31.3883299798793, Blast_Score=196, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI72000294, Length=517, Percent_Identity=24.7582205029014, Blast_Score=135, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI212646519, Length=517, Percent_Identity=24.7582205029014, Blast_Score=135, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17506189, Length=498, Percent_Identity=25.3012048192771, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17542894, Length=479, Percent_Identity=25.678496868476, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI193208247, Length=521, Percent_Identity=23.4165067178503, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI193208245, Length=521, Percent_Identity=23.4165067178503, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI45551474, Length=495, Percent_Identity=26.0606060606061, Blast_Score=158, Evalue=7e-39,
Organism=Drosophila melanogaster, GI24642670, Length=495, Percent_Identity=26.0606060606061, Blast_Score=158, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24642666, Length=495, Percent_Identity=26.0606060606061, Blast_Score=158, Evalue=1e-38,
Organism=Drosophila melanogaster, GI161077878, Length=495, Percent_Identity=26.0606060606061, Blast_Score=158, Evalue=1e-38,
Organism=Drosophila melanogaster, GI161077880, Length=495, Percent_Identity=26.0606060606061, Blast_Score=158, Evalue=1e-38,
Organism=Drosophila melanogaster, GI18859893, Length=497, Percent_Identity=26.7605633802817, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI161077496, Length=502, Percent_Identity=25.4980079681275, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI18859741, Length=490, Percent_Identity=25.9183673469388, Blast_Score=114, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000101 [H]

Pfam domain/function: PF01019 G_glu_transpept [H]

EC number: =2.3.2.2 [H]

Molecular weight: Translated: 56168; Mature: 56036

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYQFSNRQQIRKPAVTSKGGIVAAQSRRAAEIGAEVLAAGGDCIDAVVATGFALGVVEP
CCCCCCCHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
WMSGLGGGGAMVLYRAKDNKVEVIDYGMRAPDSLRVEDYPLTNDGSVSPDIFPWPRVKDD
HHHCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCC
RNLHGPGAIAVPGVVAGMDMAYRRYARLPWQDLVAPSVALAAEGLLVDWWTTVMISSCAA
CCCCCCCCEECCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHH
DLRRYAGSAAAYLKDGLPPNPQWGAKAITRLPQEQLKATLAHLASTGARDFYQGDIARSL
HHHHHHCCHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
TRDIQAAGGALSVEDLAAFAAQVRDPLRIPYRGGAVFATPELTAGPTLAHALRLMQTSIE
HHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCC
PAGKAPDAGAFIAYAEAMQSAYRERLNDMGDAPGKRALGAEYLAPACTTHYSVVDRDGNM
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHCCEEEECCCCH
AAVTQTLLSGFGSKFQAPQSGIMMNNGIMWFDPTPGTTNSLAPGKRCLTNYTPVVAETAD
HHHHHHHHHHCCCCCCCCCCCEEEECCEEEECCCCCCCCCCCCCHHHHHCCCCCEEECCC
GRRLALGASGGRRILPAVAQMLSFVMDYRMDLDAAIHQPRIDASEGDVVGADTRLPAEVM
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCEECCCCCCHHHHH
SALTAKFETVPAPVQTMPMKFACPSIVLRDGVTNSGATEIAHAWGDAVAEG
HHHHHHHHCCCCCHHHCCHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PYQFSNRQQIRKPAVTSKGGIVAAQSRRAAEIGAEVLAAGGDCIDAVVATGFALGVVEP
CCCCCCHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
WMSGLGGGGAMVLYRAKDNKVEVIDYGMRAPDSLRVEDYPLTNDGSVSPDIFPWPRVKDD
HHHCCCCCCEEEEEECCCCCEEEEECCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCC
RNLHGPGAIAVPGVVAGMDMAYRRYARLPWQDLVAPSVALAAEGLLVDWWTTVMISSCAA
CCCCCCCCEECCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHH
DLRRYAGSAAAYLKDGLPPNPQWGAKAITRLPQEQLKATLAHLASTGARDFYQGDIARSL
HHHHHHCCHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
TRDIQAAGGALSVEDLAAFAAQVRDPLRIPYRGGAVFATPELTAGPTLAHALRLMQTSIE
HHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCC
PAGKAPDAGAFIAYAEAMQSAYRERLNDMGDAPGKRALGAEYLAPACTTHYSVVDRDGNM
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHCCEEEECCCCH
AAVTQTLLSGFGSKFQAPQSGIMMNNGIMWFDPTPGTTNSLAPGKRCLTNYTPVVAETAD
HHHHHHHHHHCCCCCCCCCCCEEEECCEEEECCCCCCCCCCCCCHHHHHCCCCCEEECCC
GRRLALGASGGRRILPAVAQMLSFVMDYRMDLDAAIHQPRIDASEGDVVGADTRLPAEVM
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCEECCCCCCHHHHH
SALTAKFETVPAPVQTMPMKFACPSIVLRDGVTNSGATEIAHAWGDAVAEG
HHHHHHHHCCCCCHHHCCHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3680178 [H]