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The map label for this gene is mdlC [H]

Identifier: 146338520

GI number: 146338520

Start: 1542049

End: 1543731

Strand: Reverse

Name: mdlC [H]

Synonym: BRADO1439

Alternate gene names: 146338520

Gene position: 1543731-1542049 (Counterclockwise)

Preceding gene: 146338521

Following gene: 146338519

Centisome position: 20.7

GC content: 68.39

Gene sequence:

>1683_bases
TTGCCCGGAACTCTGGATTGCTTCGTCGCTTCGCTCCTCGCAATGACGGCAATCCGAGGGACGCCTTTGGCCAAGAAGAC
AACAACCAAGCCCGTCACCGTCAAGCAGGCCACCTTCGATCTGCTGCGCGCCTTCGGCATCAAGAAGGTGTTCGGCAACC
CCGGCTCGACCGAACTGCCATTCCTCAGCGACTGGCCCGACGATATCGACTACGTGCTCGGCCTGCAGGAGGCTTCCGTC
GTCGGCATGGCCGACGGCTATGCGCAGGCGACGCGCAATGCCGGCTTCGTCAATCTGCATTCGGCCGCCGGTGTCGGCAA
CGCGCTCGGCAACATCTACACCGCGCATCGCAACCAGACGCCGCTCGTCATCACCGCCGGCCAGCAGGCGCGCTCGATCC
TGCCGCTGCAGGCGTTCCTCTACGCCGAACGCCCGAGCGAATTCCCGCGTCCGTACGTCAAATACAGCGTCGAGCCGGCG
CGGCCCGAGGATGTGCCGGGCGCGATCGCGCGCGCCTACTACACCGCGATGCAGCCGCCCTGCGGCCCGACTTTCGTATC
GATCCCGATCGACGACTGGATGCATCCGGCGCAGCCGGTCGCGGCACGAAAGGTCAGCCGCGAGCTCGGCCCCGATCGCG
CCGCGATAGAGGAGCTGGTCGCAGCGCTCGGAGCGGCCAAGAACCCCGCGCTCGTCGTCGGCCCTGGCATCGACCGCGCC
GCTTGTGTCGACCTGATGGTCCAGGTCGCCGAGAAGGCGAAGGCCGGCGTCTGGGTCAGCCCGTTCTCGGCGCGCTGCTC
GTTCCCGGAGCGGCATCCGCAATTCCAGGGCTTCCTGCACGCCTCCCCCGGACAGCTGTCGGAGGCGCTGAAGCCGTACG
ACATCGTCGTGGTCATCGGCGCGCCGGTGTTCACCTTCCACGTCGAGGGCCATGCCGCGATCTTCGACGGCGCGACCACG
CTCTATCAGATCACCGACGATGCCGAGGGCGCGAGCGTGCCGCCGATCGGAACCAGCATCGTCGCGACGATGCGTCCCGC
GCTGTCGCTGTTGCGCGAACTCTTGCCGGAGTCGCAGCGCGCCGCGCCGAAGGGCCGCGTGATGCCGGAGCCGCCCGACG
CGTCCGACCCGATCCCGGTCGACTATCTGCTGCACACGCTGTCGCAGGCTTTGCCGCCCGGCGCCGCCATCGTCGAGGAG
ATCCCCTCGCACCGTCCGGTCATGTACAAATACATGCCGATGCCGGGCGCGGACTCGTTCTACACGATGGCGAGCGGCGG
CCTCGGCTACAGCCTGCCGGCCTCAGTCGGCATGGCGCTGGGGCGGCCGAACGACCGCATCGTCTGCCTGATCGGCGACG
GCTCGGCGATGTATTCGCTGCAGGCGCTGTGGACCGCAGCCCAGCGAAAGCTGCGGCTGACGATCGTGGTCATCAACAAT
TCCGGCTACGGCGCGATGCGCTCGTTCAGCCAGGTCATGCAGGTGCGCAACGTGCCCGGGCTCGAGCTGCCCGGCCTCGA
CTTCGTCAAGCTCGCGGAGGGCCTGGGCTGCGACGCCGTGCGGGTCAGTCGCTCGGCCGATCTGCCCGCCGCGCTCGCCC
GCGGCCTCGCCCACGACGGCACCAGCCTCGTGGAGGTGATGGTCGATTCCGCGGTGCCGCTGCTCTATGCGCAGAAGCGC
TGA

Upstream 100 bases:

>100_bases
CTGTGGACCGCCAATGCCGAGCGCCAGCCGACCATGAAGGGCACGGCGACGTGGTGATACAGTCATCGTCATTGCGAGCG
AAGCGAAGCAATCCAGGGCT

Downstream 100 bases:

>100_bases
GCTCGCGTTCGCTGAGCGGACGCGCTGTCAATCTCGGATGATGCGATAATACGTCGCATCATCCGTGGCACGGCCATGGC
TGGCCGCCGAATGCCCACAA

Product: benzoylformate decarboxylase

Products: NA

Alternate protein names: BFD; BFDC [H]

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MPGTLDCFVASLLAMTAIRGTPLAKKTTTKPVTVKQATFDLLRAFGIKKVFGNPGSTELPFLSDWPDDIDYVLGLQEASV
VGMADGYAQATRNAGFVNLHSAAGVGNALGNIYTAHRNQTPLVITAGQQARSILPLQAFLYAERPSEFPRPYVKYSVEPA
RPEDVPGAIARAYYTAMQPPCGPTFVSIPIDDWMHPAQPVAARKVSRELGPDRAAIEELVAALGAAKNPALVVGPGIDRA
ACVDLMVQVAEKAKAGVWVSPFSARCSFPERHPQFQGFLHASPGQLSEALKPYDIVVVIGAPVFTFHVEGHAAIFDGATT
LYQITDDAEGASVPPIGTSIVATMRPALSLLRELLPESQRAAPKGRVMPEPPDASDPIPVDYLLHTLSQALPPGAAIVEE
IPSHRPVMYKYMPMPGADSFYTMASGGLGYSLPASVGMALGRPNDRIVCLIGDGSAMYSLQALWTAAQRKLRLTIVVINN
SGYGAMRSFSQVMQVRNVPGLELPGLDFVKLAEGLGCDAVRVSRSADLPAALARGLAHDGTSLVEVMVDSAVPLLYAQKR

Sequences:

>Translated_560_residues
MPGTLDCFVASLLAMTAIRGTPLAKKTTTKPVTVKQATFDLLRAFGIKKVFGNPGSTELPFLSDWPDDIDYVLGLQEASV
VGMADGYAQATRNAGFVNLHSAAGVGNALGNIYTAHRNQTPLVITAGQQARSILPLQAFLYAERPSEFPRPYVKYSVEPA
RPEDVPGAIARAYYTAMQPPCGPTFVSIPIDDWMHPAQPVAARKVSRELGPDRAAIEELVAALGAAKNPALVVGPGIDRA
ACVDLMVQVAEKAKAGVWVSPFSARCSFPERHPQFQGFLHASPGQLSEALKPYDIVVVIGAPVFTFHVEGHAAIFDGATT
LYQITDDAEGASVPPIGTSIVATMRPALSLLRELLPESQRAAPKGRVMPEPPDASDPIPVDYLLHTLSQALPPGAAIVEE
IPSHRPVMYKYMPMPGADSFYTMASGGLGYSLPASVGMALGRPNDRIVCLIGDGSAMYSLQALWTAAQRKLRLTIVVINN
SGYGAMRSFSQVMQVRNVPGLELPGLDFVKLAEGLGCDAVRVSRSADLPAALARGLAHDGTSLVEVMVDSAVPLLYAQKR
>Mature_559_residues
PGTLDCFVASLLAMTAIRGTPLAKKTTTKPVTVKQATFDLLRAFGIKKVFGNPGSTELPFLSDWPDDIDYVLGLQEASVV
GMADGYAQATRNAGFVNLHSAAGVGNALGNIYTAHRNQTPLVITAGQQARSILPLQAFLYAERPSEFPRPYVKYSVEPAR
PEDVPGAIARAYYTAMQPPCGPTFVSIPIDDWMHPAQPVAARKVSRELGPDRAAIEELVAALGAAKNPALVVGPGIDRAA
CVDLMVQVAEKAKAGVWVSPFSARCSFPERHPQFQGFLHASPGQLSEALKPYDIVVVIGAPVFTFHVEGHAAIFDGATTL
YQITDDAEGASVPPIGTSIVATMRPALSLLRELLPESQRAAPKGRVMPEPPDASDPIPVDYLLHTLSQALPPGAAIVEEI
PSHRPVMYKYMPMPGADSFYTMASGGLGYSLPASVGMALGRPNDRIVCLIGDGSAMYSLQALWTAAQRKLRLTIVVINNS
GYGAMRSFSQVMQVRNVPGLELPGLDFVKLAEGLGCDAVRVSRSADLPAALARGLAHDGTSLVEVMVDSAVPLLYAQKR

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=524, Percent_Identity=23.6641221374046, Blast_Score=91, Evalue=4e-18,
Organism=Escherichia coli, GI87081685, Length=539, Percent_Identity=26.9016697588126, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1790104, Length=542, Percent_Identity=25.2767527675277, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1786717, Length=556, Percent_Identity=22.841726618705, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1787096, Length=545, Percent_Identity=23.1192660550459, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323755, Length=586, Percent_Identity=23.3788395904437, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6321524, Length=494, Percent_Identity=21.8623481781377, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323073, Length=533, Percent_Identity=21.2007504690432, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323163, Length=528, Percent_Identity=20.8333333333333, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.7 [H]

Molecular weight: Translated: 59618; Mature: 59487

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGTLDCFVASLLAMTAIRGTPLAKKTTTKPVTVKQATFDLLRAFGIKKVFGNPGSTELP
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHCHHHHHCCCCCCCCC
FLSDWPDDIDYVLGLQEASVVGMADGYAQATRNAGFVNLHSAAGVGNALGNIYTAHRNQT
CCCCCCCCHHHHHCCCCCCEEECCCCHHHHHCCCCEEEECCCCCCCHHHHCEEECCCCCC
PLVITAGQQARSILPLQAFLYAERPSEFPRPYVKYSVEPARPEDVPGAIARAYYTAMQPP
CEEEECCHHHHHHCHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCC
CGPTFVSIPIDDWMHPAQPVAARKVSRELGPDRAAIEELVAALGAAKNPALVVGPGIDRA
CCCEEEEEEHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEECCCCHH
ACVDLMVQVAEKAKAGVWVSPFSARCSFPERHPQFQGFLHASPGQLSEALKPYDIVVVIG
HHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCEEEECCCHHHHHCCCCEEEEEEEC
APVFTFHVEGHAAIFDGATTLYQITDDAEGASVPPIGTSIVATMRPALSLLRELLPESQR
CCEEEEEECCCEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHH
AAPKGRVMPEPPDASDPIPVDYLLHTLSQALPPGAAIVEEIPSHRPVMYKYMPMPGADSF
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEECCCCCCCE
YTMASGGLGYSLPASVGMALGRPNDRIVCLIGDGSAMYSLQALWTAAQRKLRLTIVVINN
EEEECCCCCCCCHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHCCEEEEEEEEEEC
SGYGAMRSFSQVMQVRNVPGLELPGLDFVKLAEGLGCDAVRVSRSADLPAALARGLAHDG
CCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCEEECCCCCCHHHHHHHHCCCC
TSLVEVMVDSAVPLLYAQKR
HHHHHHHHHCCCCCEEECCC
>Mature Secondary Structure 
PGTLDCFVASLLAMTAIRGTPLAKKTTTKPVTVKQATFDLLRAFGIKKVFGNPGSTELP
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHCHHHHHCCCCCCCCC
FLSDWPDDIDYVLGLQEASVVGMADGYAQATRNAGFVNLHSAAGVGNALGNIYTAHRNQT
CCCCCCCCHHHHHCCCCCCEEECCCCHHHHHCCCCEEEECCCCCCCHHHHCEEECCCCCC
PLVITAGQQARSILPLQAFLYAERPSEFPRPYVKYSVEPARPEDVPGAIARAYYTAMQPP
CEEEECCHHHHHHCHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCC
CGPTFVSIPIDDWMHPAQPVAARKVSRELGPDRAAIEELVAALGAAKNPALVVGPGIDRA
CCCEEEEEEHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEECCCCHH
ACVDLMVQVAEKAKAGVWVSPFSARCSFPERHPQFQGFLHASPGQLSEALKPYDIVVVIG
HHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCEEEECCCHHHHHCCCCEEEEEEEC
APVFTFHVEGHAAIFDGATTLYQITDDAEGASVPPIGTSIVATMRPALSLLRELLPESQR
CCEEEEEECCCEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHH
AAPKGRVMPEPPDASDPIPVDYLLHTLSQALPPGAAIVEEIPSHRPVMYKYMPMPGADSF
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEECCCCCCCE
YTMASGGLGYSLPASVGMALGRPNDRIVCLIGDGSAMYSLQALWTAAQRKLRLTIVVINN
EEEECCCCCCCCHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHCCEEEEEEEEEEC
SGYGAMRSFSQVMQVRNVPGLELPGLDFVKLAEGLGCDAVRVSRSADLPAALARGLAHDG
CCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCEEECCCCCCHHHHHHHHCCCC
TSLVEVMVDSAVPLLYAQKR
HHHHHHHHHCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2271624; 7663351; 9665697 [H]