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The map label for this gene is yfcG [H]

Identifier: 146337864

GI number: 146337864

Start: 788597

End: 789223

Strand: Direct

Name: yfcG [H]

Synonym: BRADO0749

Alternate gene names: 146337864

Gene position: 788597-789223 (Clockwise)

Preceding gene: 146337861

Following gene: 146337869

Centisome position: 10.58

GC content: 66.51

Gene sequence:

>627_bases
ATGACGATCGAACTGCACACCTGGAACACCCCCAACGGCCGCAAGATCTCGGTGGCGCTGGAGGAGATGGGGCTGGCTTA
TCGCGTCATTCCGGTCGACATCACCAAGGGCGCCCAGATGGCCCCGGCGTTCCTGGCGATCAGCCCGAACAACAAGATCC
CGGCGATCGTCGATCCCGCCGGACCCGAGGGCCAGCCGGTCAGCGTGTTCGAATCCGGCGCGATCCTGCTGTACCTGGCC
GAGAAGACCGGCCAATTCCTGCCGAAATCGCTCGCTGCGCGGATCCCGGTCTATGAATGGCTGATGTGGCAGATGGGCGG
CTTCGGCCCGATGCCCGGCCAGGTGCACCATTTCATCGCGCTGGAGAACGAGCAGGACCGCGCCTACGGCCTGAAGCGCT
ACATGGCCGAGACCGTCCGGCTCTATGGCGTGCTCGACCGCCGCCTGGCGCAGTACGAATTCGTCGCCGGCGCGCTCTCG
ATCGCCGATTTCGCCATCCTCGGCTGGGCCTGGCGCCACCCCCGCCACAAGGTCGACCTCGCCGATTTCCCCCACGTCAA
GCGCTGGTACGACGCGCTGATGGCCCGCCCCGCCGTGAAGCGTGGCATGGAGGCGAAGCTGGACTGA

Upstream 100 bases:

>100_bases
ACGTCCCATCAACAACGCTACGTTTGGAGCGGCGCTCAGTTTCGAGCTAATGAAGCCAGCGCCCGGCATGCACAATCCGC
AAAACCTCGGAAGGCCCCCC

Downstream 100 bases:

>100_bases
GGGAAGCCCGACGCGGATCGCAGCCGGTGATGGCGCCACACTAAACTGCTGACCATAAGCCCACCGCAAGTTGCGAATGT
CCAGCGCGCCGGTTTCGAGC

Product: putative glutathione S-transferase

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MTIELHTWNTPNGRKISVALEEMGLAYRVIPVDITKGAQMAPAFLAISPNNKIPAIVDPAGPEGQPVSVFESGAILLYLA
EKTGQFLPKSLAARIPVYEWLMWQMGGFGPMPGQVHHFIALENEQDRAYGLKRYMAETVRLYGVLDRRLAQYEFVAGALS
IADFAILGWAWRHPRHKVDLADFPHVKRWYDALMARPAVKRGMEAKLD

Sequences:

>Translated_208_residues
MTIELHTWNTPNGRKISVALEEMGLAYRVIPVDITKGAQMAPAFLAISPNNKIPAIVDPAGPEGQPVSVFESGAILLYLA
EKTGQFLPKSLAARIPVYEWLMWQMGGFGPMPGQVHHFIALENEQDRAYGLKRYMAETVRLYGVLDRRLAQYEFVAGALS
IADFAILGWAWRHPRHKVDLADFPHVKRWYDALMARPAVKRGMEAKLD
>Mature_207_residues
TIELHTWNTPNGRKISVALEEMGLAYRVIPVDITKGAQMAPAFLAISPNNKIPAIVDPAGPEGQPVSVFESGAILLYLAE
KTGQFLPKSLAARIPVYEWLMWQMGGFGPMPGQVHHFIALENEQDRAYGLKRYMAETVRLYGVLDRRLAQYEFVAGALSI
ADFAILGWAWRHPRHKVDLADFPHVKRWYDALMARPAVKRGMEAKLD

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=203, Percent_Identity=49.7536945812808, Blast_Score=184, Evalue=4e-48,
Organism=Escherichia coli, GI87082195, Length=219, Percent_Identity=38.8127853881278, Blast_Score=138, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6324100, Length=236, Percent_Identity=30.5084745762712, Blast_Score=105, Evalue=5e-24,
Organism=Drosophila melanogaster, GI28571670, Length=173, Percent_Identity=28.3236994219653, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI17864592, Length=182, Percent_Identity=29.1208791208791, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI17933730, Length=203, Percent_Identity=27.5862068965517, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 23342; Mature: 23211

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIELHTWNTPNGRKISVALEEMGLAYRVIPVDITKGAQMAPAFLAISPNNKIPAIVDPA
CEEEEEECCCCCCCEEEEEEHHCCCEEEEEEEEECCCCCCCCEEEEECCCCCCCEEECCC
GPEGQPVSVFESGAILLYLAEKTGQFLPKSLAARIPVYEWLMWQMGGFGPMPGQVHHFIA
CCCCCCEEEECCCEEEEEEECCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEEEE
LENEQDRAYGLKRYMAETVRLYGVLDRRLAQYEFVAGALSIADFAILGWAWRHPRHKVDL
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ADFPHVKRWYDALMARPAVKRGMEAKLD
CCCCHHHHHHHHHHHCHHHHCCCCCCCC
>Mature Secondary Structure 
TIELHTWNTPNGRKISVALEEMGLAYRVIPVDITKGAQMAPAFLAISPNNKIPAIVDPA
EEEEEECCCCCCCEEEEEEHHCCCEEEEEEEEECCCCCCCCEEEEECCCCCCCEEECCC
GPEGQPVSVFESGAILLYLAEKTGQFLPKSLAARIPVYEWLMWQMGGFGPMPGQVHHFIA
CCCCCCEEEECCCEEEEEEECCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEEEE
LENEQDRAYGLKRYMAETVRLYGVLDRRLAQYEFVAGALSIADFAILGWAWRHPRHKVDL
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ADFPHVKRWYDALMARPAVKRGMEAKLD
CCCCHHHHHHHHHHHCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]