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The map label for this gene is yjeP [H]

Identifier: 146337839

GI number: 146337839

Start: 758066

End: 759394

Strand: Reverse

Name: yjeP [H]

Synonym: BRADO0720

Alternate gene names: 146337839

Gene position: 759394-758066 (Counterclockwise)

Preceding gene: 146337841

Following gene: 146337838

Centisome position: 10.18

GC content: 64.56

Gene sequence:

>1329_bases
ATGGACCTCGATCTACTCGATTTGAAAGACGTTCTCGAATTCCTCCAGACTGTTGCCCGATCGATCGGCGCCGAGGTCAC
CTCACCCTGGTTCTATCTTCAGCTCGGATTGGTGCTGAGCGGCGCCGGGATCGCGTTCGCGGCGAGCGCCGCGGTGCGCG
CGCGTATCGACGTGACCTCGCTGGCGATGGGCTGGCCGGGTCCGCTGCGGCAGCTGCTGCGCGTGCTGCTGGAGAGCGTC
ACCACCGTCGTGTTCGTCATCCTGATGCGGATCGCGCGCACCGGCATGGTGCTGTGGACCTGGCCGAGCCGCTCCTATCT
GCTCGCGGTTGCGCTCAAGCTGGCGCTGGCCTGGCTTGTCATCCGGCTGATCACCTCTGTGATCCGCAGCACCTTCATCG
TGCAGATCGTGTCGATCTCGGCCTGGCTGGTGGCGGCGCTCAGCATCATCGGCCAGCTCGACAACGTCGTCGACGCGCTC
GACTCTGTCTCGGTCGTGCTCGGCGGGCTCAGGTTGACGCCGCTGCTGGTCATCAAGCTTGGCGTGCTGCTGTTGGCCGT
GCTCTGGCTGACCAACATCGCCAGCAATTTCGTCGAGAGCCGGATCACGCGCTCGACCGACCTGACGCCGTCGATCCAGG
TGCTGCTGATCAAGATGGTCCGGATGGGCCTGATGATCCTCGGCGTGGCGATCGCAATCAGCGCGGTCGGCATCAACCTC
TCGGCGCTCGCCATCTTCTCCGGCGCCGCCGGTGTCGGTATCGGTCTGGGCTTGCAACGCATTGTCGCCAACTTCGTCGC
CGGCATCATCCTGCTCGCCGACAAGTCGGTGAAGCCCGGCGACCTCGTCACCATCGGCGACAGCTCGGGACGAATCAGCG
CGATGAACACGCGCTACATCTCGGTCGCCGCCGGCGACGGCCGCGAGTTCCTGATCCCGAACGAGGACCTCGTCACGCAG
AAGGTCACCAACTGGACCTACACCGACAAGAACACGCTGGTGAAGGTCAATTTCGGCACCAACTACGACGCCGATCCCCG
GATCGTCGTCAAGCTCGCCACCGACATCGCGGCCGAGATGCCCCGCACGCTGAAGTCCAAGGCGCCGAACTGCCTGCTGA
CCGAGTTCACCGAGGCCGGCATGAAGTTCTCGCTGACCTGCTGGATCGCCGACCCCAGCTACATGGACAGCGTCAAGAGC
GAGGTGATGCTGGCGCTGTGGGATGCTTTCAAGCGCGAAGGCATTCGCGTCCCTTATCCGGTGCGCGAACTGCGCGTGCG
CGGCGGCGCATTGCCGGTCGAAACCACCGTCGAGGTTCCGACGGCCTGA

Upstream 100 bases:

>100_bases
TGTTCGGGGTATATTTCGCGGCAAAACCACATCACGAAAACGCGCTAAATCCGACGCGTCGGTCAGCCCCGCGCCCCTGT
TCCTCGCCGAGACCCCTCTG

Downstream 100 bases:

>100_bases
GGACGCCGACTTTTCCCTATCCCCCGTGGCATCGGCTCACTCCTCAAGCGTTTGAGTTCATCAAGCCGCTCCGCATTCCG
CGACGCCCTATCCCCCCGCT

Product: mechanosensitive ion channel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MDLDLLDLKDVLEFLQTVARSIGAEVTSPWFYLQLGLVLSGAGIAFAASAAVRARIDVTSLAMGWPGPLRQLLRVLLESV
TTVVFVILMRIARTGMVLWTWPSRSYLLAVALKLALAWLVIRLITSVIRSTFIVQIVSISAWLVAALSIIGQLDNVVDAL
DSVSVVLGGLRLTPLLVIKLGVLLLAVLWLTNIASNFVESRITRSTDLTPSIQVLLIKMVRMGLMILGVAIAISAVGINL
SALAIFSGAAGVGIGLGLQRIVANFVAGIILLADKSVKPGDLVTIGDSSGRISAMNTRYISVAAGDGREFLIPNEDLVTQ
KVTNWTYTDKNTLVKVNFGTNYDADPRIVVKLATDIAAEMPRTLKSKAPNCLLTEFTEAGMKFSLTCWIADPSYMDSVKS
EVMLALWDAFKREGIRVPYPVRELRVRGGALPVETTVEVPTA

Sequences:

>Translated_442_residues
MDLDLLDLKDVLEFLQTVARSIGAEVTSPWFYLQLGLVLSGAGIAFAASAAVRARIDVTSLAMGWPGPLRQLLRVLLESV
TTVVFVILMRIARTGMVLWTWPSRSYLLAVALKLALAWLVIRLITSVIRSTFIVQIVSISAWLVAALSIIGQLDNVVDAL
DSVSVVLGGLRLTPLLVIKLGVLLLAVLWLTNIASNFVESRITRSTDLTPSIQVLLIKMVRMGLMILGVAIAISAVGINL
SALAIFSGAAGVGIGLGLQRIVANFVAGIILLADKSVKPGDLVTIGDSSGRISAMNTRYISVAAGDGREFLIPNEDLVTQ
KVTNWTYTDKNTLVKVNFGTNYDADPRIVVKLATDIAAEMPRTLKSKAPNCLLTEFTEAGMKFSLTCWIADPSYMDSVKS
EVMLALWDAFKREGIRVPYPVRELRVRGGALPVETTVEVPTA
>Mature_442_residues
MDLDLLDLKDVLEFLQTVARSIGAEVTSPWFYLQLGLVLSGAGIAFAASAAVRARIDVTSLAMGWPGPLRQLLRVLLESV
TTVVFVILMRIARTGMVLWTWPSRSYLLAVALKLALAWLVIRLITSVIRSTFIVQIVSISAWLVAALSIIGQLDNVVDAL
DSVSVVLGGLRLTPLLVIKLGVLLLAVLWLTNIASNFVESRITRSTDLTPSIQVLLIKMVRMGLMILGVAIAISAVGINL
SALAIFSGAAGVGIGLGLQRIVANFVAGIILLADKSVKPGDLVTIGDSSGRISAMNTRYISVAAGDGREFLIPNEDLVTQ
KVTNWTYTDKNTLVKVNFGTNYDADPRIVVKLATDIAAEMPRTLKSKAPNCLLTEFTEAGMKFSLTCWIADPSYMDSVKS
EVMLALWDAFKREGIRVPYPVRELRVRGGALPVETTVEVPTA

Specific function: Unknown

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI2367355, Length=312, Percent_Identity=28.525641025641, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1786670, Length=165, Percent_Identity=32.1212121212121, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789291, Length=223, Percent_Identity=24.2152466367713, Blast_Score=82, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 47705; Mature: 47705

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLDLLDLKDVLEFLQTVARSIGAEVTSPWFYLQLGLVLSGAGIAFAASAAVRARIDVTS
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEHHHHHCCCCHHHHHHHHHHEEEEHHH
LAMGWPGPLRQLLRVLLESVTTVVFVILMRIARTGMVLWTWPSRSYLLAVALKLALAWLV
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHH
IRLITSVIRSTFIVQIVSISAWLVAALSIIGQLDNVVDALDSVSVVLGGLRLTPLLVIKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
GVLLLAVLWLTNIASNFVESRITRSTDLTPSIQVLLIKMVRMGLMILGVAIAISAVGINL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
SALAIFSGAAGVGIGLGLQRIVANFVAGIILLADKSVKPGDLVTIGDSSGRISAMNTRYI
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEECCCCCCEEEEEEEEE
SVAAGDGREFLIPNEDLVTQKVTNWTYTDKNTLVKVNFGTNYDADPRIVVKLATDIAAEM
EEEECCCCEEECCCCHHHHHHHCCCEEECCCEEEEEECCCCCCCCCEEEEEEHHHHHHHC
PRTLKSKAPNCLLTEFTEAGMKFSLTCWIADPSYMDSVKSEVMLALWDAFKREGIRVPYP
CHHHHHCCCCCCHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VRELRVRGGALPVETTVEVPTA
HHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDLDLLDLKDVLEFLQTVARSIGAEVTSPWFYLQLGLVLSGAGIAFAASAAVRARIDVTS
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEHHHHHCCCCHHHHHHHHHHEEEEHHH
LAMGWPGPLRQLLRVLLESVTTVVFVILMRIARTGMVLWTWPSRSYLLAVALKLALAWLV
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHH
IRLITSVIRSTFIVQIVSISAWLVAALSIIGQLDNVVDALDSVSVVLGGLRLTPLLVIKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
GVLLLAVLWLTNIASNFVESRITRSTDLTPSIQVLLIKMVRMGLMILGVAIAISAVGINL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
SALAIFSGAAGVGIGLGLQRIVANFVAGIILLADKSVKPGDLVTIGDSSGRISAMNTRYI
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEECCCCCCEEEEEEEEE
SVAAGDGREFLIPNEDLVTQKVTNWTYTDKNTLVKVNFGTNYDADPRIVVKLATDIAAEM
EEEECCCCEEECCCCHHHHHHHCCCEEECCCEEEEEECCCCCCCCCEEEEEEHHHHHHHC
PRTLKSKAPNCLLTEFTEAGMKFSLTCWIADPSYMDSVKSEVMLALWDAFKREGIRVPYP
CHHHHHCCCCCCHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VRELRVRGGALPVETTVEVPTA
HHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 3042771 [H]