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The map label for this gene is appB [H]

Identifier: 146337751

GI number: 146337751

Start: 650399

End: 651370

Strand: Direct

Name: appB [H]

Synonym: BRADO0626

Alternate gene names: 146337751

Gene position: 650399-651370 (Clockwise)

Preceding gene: 146337750

Following gene: 146337752

Centisome position: 8.72

GC content: 66.15

Gene sequence:

>972_bases
ATGCTCTCCTTCATCGTCCAGCGCGTCGCGAAGGGCGTCATCGTCCTTCTCGCCATCGTCGTGCTGAACTTCTTCCTGAT
CCGGCTGGCGCCGGGGGATCCCGCCGTCGTGATGGCCGGCGAAGCCGGCGCGTCCGACCAGATGTTCGTCGCGCAGCTGC
GGGAAAAATTCGGCCTCGACCGTCCGCTGCCGGAGCAGCTGCTCATTTACATCAAGGGCATCGCCAGCCTCGATCTCGGC
TTCTCGTTCCGCCAGCAGGCGCCGGTCGCCAAGCTGATCGTCGAGCGGCTTCCGGCGACGCTGCTGCTGACGCTCTCTGC
ATTCGCGATCTCGCTCGTGCTCGGCGTCCTGTTCGGTGCGCTGGCGGCAAGGTTCGCCGGCACCTTCCTCGATACGGCAA
TCACCGTGCTCGCGCTGATCTTTTACGCGACGCCGCTGTTCTGGATCGCGCTGATCGCGATCCTGCTGTTCTCCGTCTGG
GTCGATTGGCTGCCGAGCTTCGGCTACGAGACCGTTGGCGCCGGCTACACCGGCCTGCGCCACGCGCTCGACGTCGGCGC
GCATCTGGTGATGCCCGCAGCGACGCTCGGCCTGTTCTTCATGGCGACCTACACCCGCATGACCCGCGCCTCGATGCTCG
AGGTGAAACGGCTCGACTTCGTCAAGACCGCGCGCGCCAAGGGCCTGCGCGACGGCGTCATCCTGCGGCGTCACGTGCTG
CGCAACGCGCTGCTGCCGGTGGTGACATTGGCCGGCGTCCACGCCGGCACCCTGGTCGGCGGCGCCGTGCTGACGGAGAC
CGTGTTCGCCTGGCCCGGCATCGGCAGGCTGATGTACGACGCGCTGATTCAGCGCGACTACAATCTCCTGCTTGGCGTGT
TCGTGGTCTCCTCGGCCATGGTGCTGATCTTCAACCTGGTCACCGACCTCGTCTATCGCCTGGTCGACCCGCGCATCGAG
TATGCGTCATGA

Upstream 100 bases:

>100_bases
CGCAAAACTCCGATGACAAACAATAGTCCTGGGGCGCTGCGGTGTGCGCTGCGCCCCAGGTGCTCCACCTGCATCTCGAG
CTGACCATCACGGGCCCTCC

Downstream 100 bases:

>100_bases
GGCAGTTCTGGCGCACCATGCTGCGCAGCCCGAGCGGCATCATCGGGCTGATCATCCTGATCCTTGCGATCGTGATCGCG
GTGGCCGGACCGTTCTATTT

Product: putative dipeptide transport system permease

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MLSFIVQRVAKGVIVLLAIVVLNFFLIRLAPGDPAVVMAGEAGASDQMFVAQLREKFGLDRPLPEQLLIYIKGIASLDLG
FSFRQQAPVAKLIVERLPATLLLTLSAFAISLVLGVLFGALAARFAGTFLDTAITVLALIFYATPLFWIALIAILLFSVW
VDWLPSFGYETVGAGYTGLRHALDVGAHLVMPAATLGLFFMATYTRMTRASMLEVKRLDFVKTARAKGLRDGVILRRHVL
RNALLPVVTLAGVHAGTLVGGAVLTETVFAWPGIGRLMYDALIQRDYNLLLGVFVVSSAMVLIFNLVTDLVYRLVDPRIE
YAS

Sequences:

>Translated_323_residues
MLSFIVQRVAKGVIVLLAIVVLNFFLIRLAPGDPAVVMAGEAGASDQMFVAQLREKFGLDRPLPEQLLIYIKGIASLDLG
FSFRQQAPVAKLIVERLPATLLLTLSAFAISLVLGVLFGALAARFAGTFLDTAITVLALIFYATPLFWIALIAILLFSVW
VDWLPSFGYETVGAGYTGLRHALDVGAHLVMPAATLGLFFMATYTRMTRASMLEVKRLDFVKTARAKGLRDGVILRRHVL
RNALLPVVTLAGVHAGTLVGGAVLTETVFAWPGIGRLMYDALIQRDYNLLLGVFVVSSAMVLIFNLVTDLVYRLVDPRIE
YAS
>Mature_323_residues
MLSFIVQRVAKGVIVLLAIVVLNFFLIRLAPGDPAVVMAGEAGASDQMFVAQLREKFGLDRPLPEQLLIYIKGIASLDLG
FSFRQQAPVAKLIVERLPATLLLTLSAFAISLVLGVLFGALAARFAGTFLDTAITVLALIFYATPLFWIALIAILLFSVW
VDWLPSFGYETVGAGYTGLRHALDVGAHLVMPAATLGLFFMATYTRMTRASMLEVKRLDFVKTARAKGLRDGVILRRHVL
RNALLPVVTLAGVHAGTLVGGAVLTETVFAWPGIGRLMYDALIQRDYNLLLGVFVVSSAMVLIFNLVTDLVYRLVDPRIE
YAS

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789965, Length=342, Percent_Identity=39.1812865497076, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1787053, Length=321, Percent_Identity=34.5794392523364, Blast_Score=191, Evalue=5e-50,
Organism=Escherichia coli, GI1789888, Length=322, Percent_Identity=34.7826086956522, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI1787497, Length=324, Percent_Identity=33.0246913580247, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1787761, Length=332, Percent_Identity=36.144578313253, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1787550, Length=265, Percent_Identity=32.0754716981132, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1788504, Length=367, Percent_Identity=29.4277929155313, Blast_Score=136, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35198; Mature: 35198

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSFIVQRVAKGVIVLLAIVVLNFFLIRLAPGDPAVVMAGEAGASDQMFVAQLREKFGLD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCC
RPLPEQLLIYIKGIASLDLGFSFRQQAPVAKLIVERLPATLLLTLSAFAISLVLGVLFGA
CCCHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
LAARFAGTFLDTAITVLALIFYATPLFWIALIAILLFSVWVDWLPSFGYETVGAGYTGLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHH
HALDVGAHLVMPAATLGLFFMATYTRMTRASMLEVKRLDFVKTARAKGLRDGVILRRHVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RNALLPVVTLAGVHAGTLVGGAVLTETVFAWPGIGRLMYDALIQRDYNLLLGVFVVSSAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLIFNLVTDLVYRLVDPRIEYAS
HHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLSFIVQRVAKGVIVLLAIVVLNFFLIRLAPGDPAVVMAGEAGASDQMFVAQLREKFGLD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCC
RPLPEQLLIYIKGIASLDLGFSFRQQAPVAKLIVERLPATLLLTLSAFAISLVLGVLFGA
CCCHHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
LAARFAGTFLDTAITVLALIFYATPLFWIALIAILLFSVWVDWLPSFGYETVGAGYTGLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHH
HALDVGAHLVMPAATLGLFFMATYTRMTRASMLEVKRLDFVKTARAKGLRDGVILRRHVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
RNALLPVVTLAGVHAGTLVGGAVLTETVFAWPGIGRLMYDALIQRDYNLLLGVFVVSSAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLIFNLVTDLVYRLVDPRIEYAS
HHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]