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The map label for this gene is gsiB [H]

Identifier: 146337750

GI number: 146337750

Start: 648693

End: 650111

Strand: Direct

Name: gsiB [H]

Synonym: BRADO0624

Alternate gene names: 146337750

Gene position: 648693-650111 (Clockwise)

Preceding gene: 146337749

Following gene: 146337751

Centisome position: 8.7

GC content: 61.66

Gene sequence:

>1419_bases
ATGGTCCAGAACGGTCCGACCCAGATGGTGTCCGGCAACATTTTCGAAGGTCTGCTGCGCTACAGCCCGAAGCTCGAGCC
GCTGCCCGGCCTCGCCGAGAGCTGGACGATCAGCCCCGACGCCAAGGCCTATACGTTCAAGCTGAAGCCCGGCGTGACCT
GGCACGACGGCAAGCCGTTCACCTCGGCCGACGTGCTGTTCTCGATCGAGATGCTGAAGCAGACGCATGCGCGCGCCCGC
AACAACCTGGTCATGGTCGAGAAGGTCGAGGCGCCCGATGATCTCACCGTGGTGTTCACCTTGAAGGAGCCGTTCGGTCC
GTTCATCGGCATCTTCGAGGTCGGCTCGCTGCCGATGGTGCCGAAGCATCTCTACGAGGGCACCGACTACAAGACCAATC
CGAACAACAACGCGCCCATCGGTACCGGCCCCTTCATGTTCAAGGAATGGCAGAAGGGCTCGTTCATCCGCATGGTGAAA
AACCCGAACTACCATGAGAAGGGAAAGCCCTATCTCGACGAGGTCTACTGGCAGATCATTCCAGATGCGGCAGCGCGCGC
CGTGGCCTTCGAGACCGGCAAGGTCGACGTGCTGCCCGGCGGCTCGATCGAGAATTTCGACGTGCCGCGCGTCTCCAAGC
TGCCGGGCGCATGCGTCACCGGCGCTGGCTGGGAATTTTTCTCGCCGCTCTCCTGGATGTGGTTGAACCTGCGCAACCCG
ATTCTCGCCAACAAGAAAGTCCGGCAGGCGATCATGTACGCCATCGATCGCGACTTCGCCAAGGACGTGATCTGGAACGG
GCTCGGCAAGGTGGCGACAGGCCCGTCGGCCTCGACCATCAAGTTCTACACCGATGACGTACCGAAGTACTCCTATGATC
CCGCCAAGGCCAAGGCGCTGCTGAAGGAGGCCGGCTACAAGGGCGAGAAGATCCGCATGCTGCCGCTGGCCTATGGCGAG
ACTTGGCAGCGCTGGGGCGAGGCCGTGAAGCAGAACCTCCAGGACGTCGGCATGACGATCGAGACGATCGCCACCGACGT
GCCCGGCGGCAATCAGAAGATCATGGAGTGGGACTACGACCTCGCCTTCACCTATCTCTATCAGTATGGTGATCCCGCGC
TCGGTGTCTCGCGCAACTACATCACCTCGGCCATCGCCAAGGGCCAGCTGTTCAACAATGTCGAGGGCTATTCGAACCCC
GAGGTCGACAAGCTGTTCGCCGAAGGCGCGACGGCACCGACCGATGCCAAGCGCAAGGAGGCCTACGACAAGGTGCAGAA
GATCCTCGTCGAGGACGTGCCGGTGGCCTGGCTGTTGGAGCTGCAGTTCCCGACCATCACCAAATGCAAGGTCAAGAACC
TGATCACGACCGGCGTCGGCGTCAACGACGGCTTCCGCGACGCCTGGCTCGACAAATGA

Upstream 100 bases:

>100_bases
CATCGGCTGCGGATTGTCCGCAGCTCATGCGGAGGAGCCGAAGATCGGCGGCGTCGTGAACGCCGTCATCCAGCCGGAGC
CGCCGGGGTTGATGCTGGCA

Downstream 100 bases:

>100_bases
TGGCAGGATTCGGAGCGCATCCGGGACGCTTGTGTATTGACGTATTGGGCTGTCGCGGCATGCCATCCGGCCAGTTTGTT
ACTCCGTCATGCCCGGGCTT

Product: putative dipeptide ABC transporter substrate binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MVQNGPTQMVSGNIFEGLLRYSPKLEPLPGLAESWTISPDAKAYTFKLKPGVTWHDGKPFTSADVLFSIEMLKQTHARAR
NNLVMVEKVEAPDDLTVVFTLKEPFGPFIGIFEVGSLPMVPKHLYEGTDYKTNPNNNAPIGTGPFMFKEWQKGSFIRMVK
NPNYHEKGKPYLDEVYWQIIPDAAARAVAFETGKVDVLPGGSIENFDVPRVSKLPGACVTGAGWEFFSPLSWMWLNLRNP
ILANKKVRQAIMYAIDRDFAKDVIWNGLGKVATGPSASTIKFYTDDVPKYSYDPAKAKALLKEAGYKGEKIRMLPLAYGE
TWQRWGEAVKQNLQDVGMTIETIATDVPGGNQKIMEWDYDLAFTYLYQYGDPALGVSRNYITSAIAKGQLFNNVEGYSNP
EVDKLFAEGATAPTDAKRKEAYDKVQKILVEDVPVAWLLELQFPTITKCKVKNLITTGVGVNDGFRDAWLDK

Sequences:

>Translated_472_residues
MVQNGPTQMVSGNIFEGLLRYSPKLEPLPGLAESWTISPDAKAYTFKLKPGVTWHDGKPFTSADVLFSIEMLKQTHARAR
NNLVMVEKVEAPDDLTVVFTLKEPFGPFIGIFEVGSLPMVPKHLYEGTDYKTNPNNNAPIGTGPFMFKEWQKGSFIRMVK
NPNYHEKGKPYLDEVYWQIIPDAAARAVAFETGKVDVLPGGSIENFDVPRVSKLPGACVTGAGWEFFSPLSWMWLNLRNP
ILANKKVRQAIMYAIDRDFAKDVIWNGLGKVATGPSASTIKFYTDDVPKYSYDPAKAKALLKEAGYKGEKIRMLPLAYGE
TWQRWGEAVKQNLQDVGMTIETIATDVPGGNQKIMEWDYDLAFTYLYQYGDPALGVSRNYITSAIAKGQLFNNVEGYSNP
EVDKLFAEGATAPTDAKRKEAYDKVQKILVEDVPVAWLLELQFPTITKCKVKNLITTGVGVNDGFRDAWLDK
>Mature_472_residues
MVQNGPTQMVSGNIFEGLLRYSPKLEPLPGLAESWTISPDAKAYTFKLKPGVTWHDGKPFTSADVLFSIEMLKQTHARAR
NNLVMVEKVEAPDDLTVVFTLKEPFGPFIGIFEVGSLPMVPKHLYEGTDYKTNPNNNAPIGTGPFMFKEWQKGSFIRMVK
NPNYHEKGKPYLDEVYWQIIPDAAARAVAFETGKVDVLPGGSIENFDVPRVSKLPGACVTGAGWEFFSPLSWMWLNLRNP
ILANKKVRQAIMYAIDRDFAKDVIWNGLGKVATGPSASTIKFYTDDVPKYSYDPAKAKALLKEAGYKGEKIRMLPLAYGE
TWQRWGEAVKQNLQDVGMTIETIATDVPGGNQKIMEWDYDLAFTYLYQYGDPALGVSRNYITSAIAKGQLFNNVEGYSNP
EVDKLFAEGATAPTDAKRKEAYDKVQKILVEDVPVAWLLELQFPTITKCKVKNLITTGVGVNDGFRDAWLDK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=357, Percent_Identity=31.3725490196078, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1787762, Length=435, Percent_Identity=27.3563218390805, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1787052, Length=471, Percent_Identity=26.7515923566879, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1789887, Length=323, Percent_Identity=30.6501547987616, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787551, Length=496, Percent_Identity=21.9758064516129, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787495, Length=479, Percent_Identity=23.3820459290188, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1789397, Length=484, Percent_Identity=25, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87081878, Length=473, Percent_Identity=24.3128964059197, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 52725; Mature: 52725

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQNGPTQMVSGNIFEGLLRYSPKLEPLPGLAESWTISPDAKAYTFKLKPGVTWHDGKPF
CCCCCCCHHHCCHHHHHHHHCCCCCCCCCCCCCCEECCCCCCEEEEEECCCCEECCCCCC
TSADVLFSIEMLKQTHARARNNLVMVEKVEAPDDLTVVFTLKEPFGPFIGIFEVGSLPMV
CCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCEEEEEEECCCCCCEEEHEECCCCCCC
PKHLYEGTDYKTNPNNNAPIGTGPFMFKEWQKGSFIRMVKNPNYHEKGKPYLDEVYWQII
HHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHC
PDAAARAVAFETGKVDVLPGGSIENFDVPRVSKLPGACVTGAGWEFFSPLSWMWLNLRNP
CHHHHHEEEEECCCEEECCCCCCCCCCCCCHHHCCCCEEECCCCHHHCCHHHHHHHCCCC
ILANKKVRQAIMYAIDRDFAKDVIWNGLGKVATGPSASTIKFYTDDVPKYSYDPAKAKAL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHH
LKEAGYKGEKIRMLPLAYGETWQRWGEAVKQNLQDVGMTIETIATDVPGGNQKIMEWDYD
HHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEEECCH
LAFTYLYQYGDPALGVSRNYITSAIAKGQLFNNVEGYSNPEVDKLFAEGATAPTDAKRKE
HEEEEHHHCCCCCCCCCHHHHHHHHHHCHHHCCCCCCCCCCHHHHHHCCCCCCCCHHHHH
AYDKVQKILVEDVPVAWLLELQFPTITKCKVKNLITTGVGVNDGFRDAWLDK
HHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHEECCCCCCCCCHHCCCC
>Mature Secondary Structure
MVQNGPTQMVSGNIFEGLLRYSPKLEPLPGLAESWTISPDAKAYTFKLKPGVTWHDGKPF
CCCCCCCHHHCCHHHHHHHHCCCCCCCCCCCCCCEECCCCCCEEEEEECCCCEECCCCCC
TSADVLFSIEMLKQTHARARNNLVMVEKVEAPDDLTVVFTLKEPFGPFIGIFEVGSLPMV
CCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCEEEEEEECCCCCCEEEHEECCCCCCC
PKHLYEGTDYKTNPNNNAPIGTGPFMFKEWQKGSFIRMVKNPNYHEKGKPYLDEVYWQII
HHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHC
PDAAARAVAFETGKVDVLPGGSIENFDVPRVSKLPGACVTGAGWEFFSPLSWMWLNLRNP
CHHHHHEEEEECCCEEECCCCCCCCCCCCCHHHCCCCEEECCCCHHHCCHHHHHHHCCCC
ILANKKVRQAIMYAIDRDFAKDVIWNGLGKVATGPSASTIKFYTDDVPKYSYDPAKAKAL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHH
LKEAGYKGEKIRMLPLAYGETWQRWGEAVKQNLQDVGMTIETIATDVPGGNQKIMEWDYD
HHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEEECCH
LAFTYLYQYGDPALGVSRNYITSAIAKGQLFNNVEGYSNPEVDKLFAEGATAPTDAKRKE
HEEEEHHHCCCCCCCCCHHHHHHHHHHCHHHCCCCCCCCCCHHHHHHCCCCCCCCHHHHH
AYDKVQKILVEDVPVAWLLELQFPTITKCKVKNLITTGVGVNDGFRDAWLDK
HHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHEECCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA