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The map label for this gene is emrB [H]

Identifier: 146337674

GI number: 146337674

Start: 554354

End: 555859

Strand: Reverse

Name: emrB [H]

Synonym: BRADO0541

Alternate gene names: 146337674

Gene position: 555859-554354 (Counterclockwise)

Preceding gene: 146337675

Following gene: 146337671

Centisome position: 7.45

GC content: 65.14

Gene sequence:

>1506_bases
GTGACGATCTGCGCCATGACGGCGACGATCATGCAGGCGCTCGACACCACCATCGCCAATGTCGCCCTGCCCTACATGCA
GGGCACGCTGTCGGCCTCGACCGACCAGATCAACTGGGTGCTGACGTCCTACATCGTGGCCGCGGCGATCATGACCGCGC
CGGTGGGCTGGATCGCCAACCGTTTCGGCCGCAAGCGCACCTTCATCATCTGCTCGGCGGGCTTCACCCTTGCGTCCGTG
ATGTGCGGCCTGGCGCAGGACATCACGCAGATGGTGCTGTTCCGCCTGCTGCAGGGCGTGTTCGGCGCATCGCTGGTGCC
GCTGTCGCAGGCCGTGATGCTCGACTCCTACGCCCTGCACGAACGCGCCAAGGCGATGGCGATCTGGGGCATGGGCGTGA
TGCTCGGCCCGATCATGGGGCCGTCGCTGGGCGCGTGGCTGACCGAGACCTATTCCTGGCACTGGGTGTTCTTCGTCAAC
CTGCCGTTCGGCGCGGTCACCGTGCTCGGGCTCATGGCGTTCATGGAGGAGACCAAGCCGAACACCGCGCTGACCTTCGA
CTGGTTCGGCTTCATGGCGCTCGCCATCGGCATCGGCTCGCTGCAGATCGCGCTCGACCGCGGCGAGGACCAGGGCTGGC
TCGAGAGCAACGAGATCATCATCGAGTTCATCGTCGCGGCGATCGGCTTCTACTACTTCCTGGCGCATTCGCTGACCACC
AAGCGGCCGTTCATCCAGTTCGCGCTGTTCAAGGACCGCAATTTCGTCGGCGGCTGCGTGTTCATGACGGTGATGGGCCT
GGTGCTGTTCTCGACCATGGCGCTGTCCTCGCCTTACCTGCAGAACGTCATCGGCTATCCGATCATCACCGCCGGCGTGC
TGCTGGCCACCCGCGGCTGCGGCACCTTCGTGGCGATGATGCTGGTCGGGCGGCTCTTGCGCACCATCGAGGCACGCACG
CTGATCATCGTCGGGCTGTCGCTGACGGCGCTGTCGCTGTTCCAGATGACCGGCTGGACCGACCAGACCGGCGTGCCCGA
GATCGTCACCATCAGCATCATGCAGGGCTTCGGCTTCGGCCTGGTATTCGTGCCGCTCTCGACCGTGGCGTTCCTCACGC
TGCCGGGCGCGCTGCGCACCGACGGAACCTCGATGCTGACCCTGGTGCGCAATGTCGCCAGCTCGATCGGTATTTCCATT
GCCATCGCGCAGCTCACGGAAGGAACGCGGCGGAACCACGCCATCCTGGTCGAGCACATCAATCCGTTCAATCATGCTCT
GCAGATGCCGAACGTGGCGGGCCTGCTGAACCTCTCCACCGATGCCGGCCGCGCGATGGCCGACGCCATGGTCATGGTGC
AGGCGCAGATCCTCGCGTTCTCGCACGACTACCAGCTGGTGATGACGTTCATCCTGGTGTCGATCCCGCTCGCTGTGATG
ATCGGCTCGACGCGAAAGGCGCTGCGCCAGCAGTCCGCGCCGGCCGACCACGCGGTGATGGAGTAG

Upstream 100 bases:

>100_bases
GCCGGCCTGTTCGGATCGGCGCACGCGGCCGGCAAGGACGAGTAGCGCCATGACCGCGCCGGCAGCCTCATCAGCGGTTC
CGGGATTCCGCCGCAACATG

Downstream 100 bases:

>100_bases
TTAACCCAGGATGAATGTTCCCGGCGCGGAAAATACGTGTGCTATTCGGACGCAGTGCGGCGGGAGTAGCAAAAGAGTGA
CCGTGACGTGATGGATTTGT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MTICAMTATIMQALDTTIANVALPYMQGTLSASTDQINWVLTSYIVAAAIMTAPVGWIANRFGRKRTFIICSAGFTLASV
MCGLAQDITQMVLFRLLQGVFGASLVPLSQAVMLDSYALHERAKAMAIWGMGVMLGPIMGPSLGAWLTETYSWHWVFFVN
LPFGAVTVLGLMAFMEETKPNTALTFDWFGFMALAIGIGSLQIALDRGEDQGWLESNEIIIEFIVAAIGFYYFLAHSLTT
KRPFIQFALFKDRNFVGGCVFMTVMGLVLFSTMALSSPYLQNVIGYPIITAGVLLATRGCGTFVAMMLVGRLLRTIEART
LIIVGLSLTALSLFQMTGWTDQTGVPEIVTISIMQGFGFGLVFVPLSTVAFLTLPGALRTDGTSMLTLVRNVASSIGISI
AIAQLTEGTRRNHAILVEHINPFNHALQMPNVAGLLNLSTDAGRAMADAMVMVQAQILAFSHDYQLVMTFILVSIPLAVM
IGSTRKALRQQSAPADHAVME

Sequences:

>Translated_501_residues
MTICAMTATIMQALDTTIANVALPYMQGTLSASTDQINWVLTSYIVAAAIMTAPVGWIANRFGRKRTFIICSAGFTLASV
MCGLAQDITQMVLFRLLQGVFGASLVPLSQAVMLDSYALHERAKAMAIWGMGVMLGPIMGPSLGAWLTETYSWHWVFFVN
LPFGAVTVLGLMAFMEETKPNTALTFDWFGFMALAIGIGSLQIALDRGEDQGWLESNEIIIEFIVAAIGFYYFLAHSLTT
KRPFIQFALFKDRNFVGGCVFMTVMGLVLFSTMALSSPYLQNVIGYPIITAGVLLATRGCGTFVAMMLVGRLLRTIEART
LIIVGLSLTALSLFQMTGWTDQTGVPEIVTISIMQGFGFGLVFVPLSTVAFLTLPGALRTDGTSMLTLVRNVASSIGISI
AIAQLTEGTRRNHAILVEHINPFNHALQMPNVAGLLNLSTDAGRAMADAMVMVQAQILAFSHDYQLVMTFILVSIPLAVM
IGSTRKALRQQSAPADHAVME
>Mature_500_residues
TICAMTATIMQALDTTIANVALPYMQGTLSASTDQINWVLTSYIVAAAIMTAPVGWIANRFGRKRTFIICSAGFTLASVM
CGLAQDITQMVLFRLLQGVFGASLVPLSQAVMLDSYALHERAKAMAIWGMGVMLGPIMGPSLGAWLTETYSWHWVFFVNL
PFGAVTVLGLMAFMEETKPNTALTFDWFGFMALAIGIGSLQIALDRGEDQGWLESNEIIIEFIVAAIGFYYFLAHSLTTK
RPFIQFALFKDRNFVGGCVFMTVMGLVLFSTMALSSPYLQNVIGYPIITAGVLLATRGCGTFVAMMLVGRLLRTIEARTL
IIVGLSLTALSLFQMTGWTDQTGVPEIVTISIMQGFGFGLVFVPLSTVAFLTLPGALRTDGTSMLTLVRNVASSIGISIA
IAQLTEGTRRNHAILVEHINPFNHALQMPNVAGLLNLSTDAGRAMADAMVMVQAQILAFSHDYQLVMTFILVSIPLAVMI
GSTRKALRQQSAPADHAVME

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=436, Percent_Identity=35.3211009174312, Blast_Score=271, Evalue=7e-74,
Organism=Escherichia coli, GI1788710, Length=430, Percent_Identity=33.4883720930233, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1790195, Length=417, Percent_Identity=28.537170263789, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1788392, Length=398, Percent_Identity=29.8994974874372, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87081983, Length=410, Percent_Identity=26.5853658536585, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87082320, Length=167, Percent_Identity=23.9520958083832, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1790146, Length=185, Percent_Identity=25.4054054054054, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323000, Length=138, Percent_Identity=26.8115942028986, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=135, Percent_Identity=27.4074074074074, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 54253; Mature: 54122

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTICAMTATIMQALDTTIANVALPYMQGTLSASTDQINWVLTSYIVAAAIMTAPVGWIAN
CEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RFGRKRTFIICSAGFTLASVMCGLAQDITQMVLFRLLQGVFGASLVPLSQAVMLDSYALH
HCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ERAKAMAIWGMGVMLGPIMGPSLGAWLTETYSWHWVFFVNLPFGAVTVLGLMAFMEETKP
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHCCC
NTALTFDWFGFMALAIGIGSLQIALDRGEDQGWLESNEIIIEFIVAAIGFYYFLAHSLTT
CCEEEEHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
KRPFIQFALFKDRNFVGGCVFMTVMGLVLFSTMALSSPYLQNVIGYPIITAGVLLATRGC
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHCCH
GTFVAMMLVGRLLRTIEARTLIIVGLSLTALSLFQMTGWTDQTGVPEIVTISIMQGFGFG
HHHHHHHHHHHHHHHHHHCEEEEEEHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHCCCCH
LVFVPLSTVAFLTLPGALRTDGTSMLTLVRNVASSIGISIAIAQLTEGTRRNHAILVEHI
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHEEEHHHHCCCCCCCEEEEECC
NPFNHALQMPNVAGLLNLSTDAGRAMADAMVMVQAQILAFSHDYQLVMTFILVSIPLAVM
CCHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
IGSTRKALRQQSAPADHAVME
CCCHHHHHHHCCCCCCHHCCC
>Mature Secondary Structure 
TICAMTATIMQALDTTIANVALPYMQGTLSASTDQINWVLTSYIVAAAIMTAPVGWIAN
EEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RFGRKRTFIICSAGFTLASVMCGLAQDITQMVLFRLLQGVFGASLVPLSQAVMLDSYALH
HCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ERAKAMAIWGMGVMLGPIMGPSLGAWLTETYSWHWVFFVNLPFGAVTVLGLMAFMEETKP
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHCCC
NTALTFDWFGFMALAIGIGSLQIALDRGEDQGWLESNEIIIEFIVAAIGFYYFLAHSLTT
CCEEEEHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
KRPFIQFALFKDRNFVGGCVFMTVMGLVLFSTMALSSPYLQNVIGYPIITAGVLLATRGC
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHCCH
GTFVAMMLVGRLLRTIEARTLIIVGLSLTALSLFQMTGWTDQTGVPEIVTISIMQGFGFG
HHHHHHHHHHHHHHHHHHCEEEEEEHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHCCCCH
LVFVPLSTVAFLTLPGALRTDGTSMLTLVRNVASSIGISIAIAQLTEGTRRNHAILVEHI
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHEEEHHHHCCCCCCCEEEEECC
NPFNHALQMPNVAGLLNLSTDAGRAMADAMVMVQAQILAFSHDYQLVMTFILVSIPLAVM
CCHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
IGSTRKALRQQSAPADHAVME
CCCHHHHHHHCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]