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The map label for this gene is emrA [H]

Identifier: 146337675

GI number: 146337675

Start: 555915

End: 557072

Strand: Reverse

Name: emrA [H]

Synonym: BRADO0542

Alternate gene names: 146337675

Gene position: 557072-555915 (Counterclockwise)

Preceding gene: 146337676

Following gene: 146337674

Centisome position: 7.47

GC content: 67.18

Gene sequence:

>1158_bases
ATGGCTGAGCCCGCCCTGAAACTCGCCCCTGAACAGAAGGCCAGCCCGGATGCGGCGCCGCCGGCGGCTGCCGCCGCAAA
GCCGGCGCGTCCCGGCTTCCTGCGTCGCTATCGCCGCACGCTGCTGCTCGTGGTGCTCCCGCTCGTCGCCGCGATCGGCG
GCTTCGTGCTGTATCTCAATGGCGGCCGCTACGTGACCACCGACGACGCCTATGTCGGCGCGCAGAAGGTGCTCATCACG
CCCGACATCTCCGGCAAGATCATCAAGGTCCTCGTCAAGGAGGGCCAGCAGGTCGAGAAGGGCGACGAGCTGTTCGAGAT
CGACGCGGTGCCCTATCGGCTCGCGGTCGACCAGGCCAAGGCGGCGCTGGCGCAGGCGCGCACCGCCTACGACAACCTCA
CTGCCAACATCAAGATCTACGGCCAGATGCTCGACCTCGCCAATCAGGGCATCGACCTGAAGCAGAAGGACGTCGAGCGC
AAGCAGACGCTCGCCAAGAACAATTTCGGCTCGCAGCTCGATCTCGACAACGCCGCCAACGCGCTGGTGACGGCCGGCGC
CCAGGCGCAGTTCATCAAGCAGCAGCTCTCCAACGCCAAGACCCAGCTGCTCGGCAATCCCGACCTGCCGCTGGAGGAGT
TCCCGCCCTACGTCCAGGCCAAGGCCAAGCTCGAGGACGCGCAGCGCGATCTCGACCACACGACGCTGCGCGCGCCGATG
CCGGGCATCGCCACGCAGGTCGACCAGATCCAGCTGGGACGCTTCGTCGCCGCGGGCACGCCGGTGTTCTCGGTGATCGA
CATCAGCAATCCCTGGGTCGACGCCAATCCGAAGGAGAGCGACTTCACCTATGTCGCGGTCGGCCAGCCGGTCGAGCTCG
ACGTCGACGCGTTCCCGAACCACCGCTTCAAGGGCACGGTCAGTTCGCTGTCGCCGGGCACCGGCGCGCAGTTCGCGATC
CTGCCGCCGCAGAACGCCACCGGCAATTTCGTCAAGGTGGTGCAGCGCGTGCCGGTGCGGATCGCGTTCGACAAGACCGA
CAAGTTCGTCGCCAGGCTGAAGGCCGGCATGAGCGTCAACGTGTCGATCGACACCGGCCATGAGCGCACGCTCGCCGGCC
TGTTCGGATCGGCGCACGCGGCCGGCAAGGACGAGTAG

Upstream 100 bases:

>100_bases
GCCGAGGCCGAGCGGCTGATGACCCATCTCGAAGCCATCAAAGAAAATGTGAGAAACGCCCTGCAGTCCCTGTGTGACGA
GCAGCGAAAGGAGCAGCGCT

Downstream 100 bases:

>100_bases
CGCCATGACCGCGCCGGCAGCCTCATCAGCGGTTCCGGGATTCCGCCGCAACATGGTGACGATCTGCGCCATGACGGCGA
CGATCATGCAGGCGCTCGAC

Product: efflux pump protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MAEPALKLAPEQKASPDAAPPAAAAAKPARPGFLRRYRRTLLLVVLPLVAAIGGFVLYLNGGRYVTTDDAYVGAQKVLIT
PDISGKIIKVLVKEGQQVEKGDELFEIDAVPYRLAVDQAKAALAQARTAYDNLTANIKIYGQMLDLANQGIDLKQKDVER
KQTLAKNNFGSQLDLDNAANALVTAGAQAQFIKQQLSNAKTQLLGNPDLPLEEFPPYVQAKAKLEDAQRDLDHTTLRAPM
PGIATQVDQIQLGRFVAAGTPVFSVIDISNPWVDANPKESDFTYVAVGQPVELDVDAFPNHRFKGTVSSLSPGTGAQFAI
LPPQNATGNFVKVVQRVPVRIAFDKTDKFVARLKAGMSVNVSIDTGHERTLAGLFGSAHAAGKDE

Sequences:

>Translated_385_residues
MAEPALKLAPEQKASPDAAPPAAAAAKPARPGFLRRYRRTLLLVVLPLVAAIGGFVLYLNGGRYVTTDDAYVGAQKVLIT
PDISGKIIKVLVKEGQQVEKGDELFEIDAVPYRLAVDQAKAALAQARTAYDNLTANIKIYGQMLDLANQGIDLKQKDVER
KQTLAKNNFGSQLDLDNAANALVTAGAQAQFIKQQLSNAKTQLLGNPDLPLEEFPPYVQAKAKLEDAQRDLDHTTLRAPM
PGIATQVDQIQLGRFVAAGTPVFSVIDISNPWVDANPKESDFTYVAVGQPVELDVDAFPNHRFKGTVSSLSPGTGAQFAI
LPPQNATGNFVKVVQRVPVRIAFDKTDKFVARLKAGMSVNVSIDTGHERTLAGLFGSAHAAGKDE
>Mature_384_residues
AEPALKLAPEQKASPDAAPPAAAAAKPARPGFLRRYRRTLLLVVLPLVAAIGGFVLYLNGGRYVTTDDAYVGAQKVLITP
DISGKIIKVLVKEGQQVEKGDELFEIDAVPYRLAVDQAKAALAQARTAYDNLTANIKIYGQMLDLANQGIDLKQKDVERK
QTLAKNNFGSQLDLDNAANALVTAGAQAQFIKQQLSNAKTQLLGNPDLPLEEFPPYVQAKAKLEDAQRDLDHTTLRAPMP
GIATQVDQIQLGRFVAAGTPVFSVIDISNPWVDANPKESDFTYVAVGQPVELDVDAFPNHRFKGTVSSLSPGTGAQFAIL
PPQNATGNFVKVVQRVPVRIAFDKTDKFVARLKAGMSVNVSIDTGHERTLAGLFGSAHAAGKDE

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=322, Percent_Identity=32.2981366459627, Blast_Score=160, Evalue=9e-41,
Organism=Escherichia coli, GI1788711, Length=338, Percent_Identity=31.3609467455621, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1790519, Length=315, Percent_Identity=28.2539682539683, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI87081951, Length=326, Percent_Identity=25.4601226993865, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789637, Length=346, Percent_Identity=25.7225433526012, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790012, Length=270, Percent_Identity=26.2962962962963, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790024, Length=277, Percent_Identity=28.158844765343, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41361; Mature: 41229

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPALKLAPEQKASPDAAPPAAAAAKPARPGFLRRYRRTLLLVVLPLVAAIGGFVLYLN
CCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
GGRYVTTDDAYVGAQKVLITPDISGKIIKVLVKEGQQVEKGDELFEIDAVPYRLAVDQAK
CCEEEECCCCEECCEEEEEECCCCCHHHHHHHHCCCCCCCCCCEEEECCCCEEEHHHHHH
AALAQARTAYDNLTANIKIYGQMLDLANQGIDLKQKDVERKQTLAKNNFGSQLDLDNAAN
HHHHHHHHHHHCCEEEEEEEEHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHCH
ALVTAGAQAQFIKQQLSNAKTQLLGNPDLPLEEFPPYVQAKAKLEDAQRDLDHTTLRAPM
HHEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHCHHEECCCC
PGIATQVDQIQLGRFVAAGTPVFSVIDISNPWVDANPKESDFTYVAVGQPVELDVDAFPN
CCHHHHHHHHHHHHEEECCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEECCCCCC
HRFKGTVSSLSPGTGAQFAILPPQNATGNFVKVVQRVPVRIAFDKTDKFVARLKAGMSVN
CCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEECCHHHHHHHHHCCCEEE
VSIDTGHERTLAGLFGSAHAAGKDE
EEEECCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AEPALKLAPEQKASPDAAPPAAAAAKPARPGFLRRYRRTLLLVVLPLVAAIGGFVLYLN
CCCCCCCCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
GGRYVTTDDAYVGAQKVLITPDISGKIIKVLVKEGQQVEKGDELFEIDAVPYRLAVDQAK
CCEEEECCCCEECCEEEEEECCCCCHHHHHHHHCCCCCCCCCCEEEECCCCEEEHHHHHH
AALAQARTAYDNLTANIKIYGQMLDLANQGIDLKQKDVERKQTLAKNNFGSQLDLDNAAN
HHHHHHHHHHHCCEEEEEEEEHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHCH
ALVTAGAQAQFIKQQLSNAKTQLLGNPDLPLEEFPPYVQAKAKLEDAQRDLDHTTLRAPM
HHEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHCHHEECCCC
PGIATQVDQIQLGRFVAAGTPVFSVIDISNPWVDANPKESDFTYVAVGQPVELDVDAFPN
CCHHHHHHHHHHHHEEECCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEECCCCCC
HRFKGTVSSLSPGTGAQFAILPPQNATGNFVKVVQRVPVRIAFDKTDKFVARLKAGMSVN
CCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEECCHHHHHHHHHCCCEEE
VSIDTGHERTLAGLFGSAHAAGKDE
EEEECCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]