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The map label for this gene is pcaF [H]

Identifier: 146337672

GI number: 146337672

Start: 551340

End: 552548

Strand: Direct

Name: pcaF [H]

Synonym: BRADO0538

Alternate gene names: 146337672

Gene position: 551340-552548 (Clockwise)

Preceding gene: 146337670

Following gene: 146337673

Centisome position: 7.39

GC content: 69.31

Gene sequence:

>1209_bases
ATGCGAGACGTGTTCATTTGTGATGCGGTGCGGACCCCGATCGGGCGCTTCGGCGGCGCGCTGGCGAAGGTGCGCACCGA
CGATCTCGCGGCGACGCCGTTGAAGGCGTTGAAGGCCAAGCATCCGAATCTCGACTGGGCCGCGGTCGACGAGGTGTTCT
TCGGCTGCGCCAACCAGGCCGGCGAGGACAACCGCAACGTGGCGCGCATGGCGCTGCTGCTCGCAGGCCTGCCCGACTCC
ATTCCCGGCCAGACGCTCAACCGGCTCTGCGCCTCCGGCCTCGATGCGGTCGGCGCTGCCGGCCGCGCGATCCGCGCGGG
CGAGGTCGATCTCGCGATCGCCGGCGGCGTCGAATCGATGACGCGCGCGCCGTTCGTGCAGGGCAAGGCTACGGAGGCGT
TCTCGCGCTCGGCCGAGATCTTCGACACCACGATCGGCTGGCGCTTCATCAATCCGCTGATGAAGAGCCAGTATGGCGTC
GACTCGATGCCGGAGACCGGCGAGAACGTCGCCGAGGAATTCCAGGTCTCGCGCGCCGACCAGGACGCCTTCGCGATCCG
CTCCCAGCAGCGTGCCGGCCAGGCGATCGCGTCCGGCTATTTCGCCGCCGAGATCGCACAGGTCGCCGTGCCCGGTGGCA
AGGCTGGCCCCATCATCGTCGACAAGGATGAGCATCCGCGCCCCGAGACCACGCTCGAAGGTCTCGCCAAGCTGAAGCCG
ATCGTGCGCAATCCCGGCACGGTCACTGCCGGCAATGCGTCGGGCGTCAATGACGGTGCCGCCGCGATGATCCTGGCCTC
GGAAGCCGCCGTGAAGAAGCACGGCCTCACGCCGCGCGCGCGCGTGCTCGGGCTGGCGTCGGCCGGCGTGCCGCCACGCA
TCATGGGCATCGGCCCGGTGCCCGCGGTGCGCAAGCTGCTGGAGCGGCTCGGCCTCAAGATCACCGACTTCGATCTCATC
GAGCTCAACGAGGCGTTTGCTAGCCAGGGCATCGCCTGCCTGCGCCAGCTCGGCGTCGCCGAGGATGCCGATTTCGTCAA
CCCGCATGGCGGCGCCATCGCGCTCGGCCATCCCCTCGGCATGAGCGGCGCCCGCATCGCGATGACGGCGGTGCACGGCC
TCGAAGTGCGCGGGGGAAAACGCGCACTCGCCACCATGTGCGTCGGCGTCGGCCAGGGCGTTGCCCTGGCGGTCGAGATG
GTCAACTAA

Upstream 100 bases:

>100_bases
TCCACGCATGCCACCCCGCGTTCCCGTCATTTGACCTGCCCCCATCGCACTCCTAAAACCGCCGTCAATTCTGACCAGAA
ACACCCACCGGGAGAGAGCG

Downstream 100 bases:

>100_bases
CACGCCGGCGTCATTGCGAGCGAAGCGAAGCAATCCAGGGTCGCGCGCGCAGCTCTGGATTGCTTCGTCGCTTCGCTCCT
CGCAATGACGGAGTATGCCG

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MRDVFICDAVRTPIGRFGGALAKVRTDDLAATPLKALKAKHPNLDWAAVDEVFFGCANQAGEDNRNVARMALLLAGLPDS
IPGQTLNRLCASGLDAVGAAGRAIRAGEVDLAIAGGVESMTRAPFVQGKATEAFSRSAEIFDTTIGWRFINPLMKSQYGV
DSMPETGENVAEEFQVSRADQDAFAIRSQQRAGQAIASGYFAAEIAQVAVPGGKAGPIIVDKDEHPRPETTLEGLAKLKP
IVRNPGTVTAGNASGVNDGAAAMILASEAAVKKHGLTPRARVLGLASAGVPPRIMGIGPVPAVRKLLERLGLKITDFDLI
ELNEAFASQGIACLRQLGVAEDADFVNPHGGAIALGHPLGMSGARIAMTAVHGLEVRGGKRALATMCVGVGQGVALAVEM
VN

Sequences:

>Translated_402_residues
MRDVFICDAVRTPIGRFGGALAKVRTDDLAATPLKALKAKHPNLDWAAVDEVFFGCANQAGEDNRNVARMALLLAGLPDS
IPGQTLNRLCASGLDAVGAAGRAIRAGEVDLAIAGGVESMTRAPFVQGKATEAFSRSAEIFDTTIGWRFINPLMKSQYGV
DSMPETGENVAEEFQVSRADQDAFAIRSQQRAGQAIASGYFAAEIAQVAVPGGKAGPIIVDKDEHPRPETTLEGLAKLKP
IVRNPGTVTAGNASGVNDGAAAMILASEAAVKKHGLTPRARVLGLASAGVPPRIMGIGPVPAVRKLLERLGLKITDFDLI
ELNEAFASQGIACLRQLGVAEDADFVNPHGGAIALGHPLGMSGARIAMTAVHGLEVRGGKRALATMCVGVGQGVALAVEM
VN
>Mature_402_residues
MRDVFICDAVRTPIGRFGGALAKVRTDDLAATPLKALKAKHPNLDWAAVDEVFFGCANQAGEDNRNVARMALLLAGLPDS
IPGQTLNRLCASGLDAVGAAGRAIRAGEVDLAIAGGVESMTRAPFVQGKATEAFSRSAEIFDTTIGWRFINPLMKSQYGV
DSMPETGENVAEEFQVSRADQDAFAIRSQQRAGQAIASGYFAAEIAQVAVPGGKAGPIIVDKDEHPRPETTLEGLAKLKP
IVRNPGTVTAGNASGVNDGAAAMILASEAAVKKHGLTPRARVLGLASAGVPPRIMGIGPVPAVRKLLERLGLKITDFDLI
ELNEAFASQGIACLRQLGVAEDADFVNPHGGAIALGHPLGMSGARIAMTAVHGLEVRGGKRALATMCVGVGQGVALAVEM
VN

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=403, Percent_Identity=42.1836228287841, Blast_Score=284, Evalue=1e-76,
Organism=Homo sapiens, GI148539872, Length=398, Percent_Identity=40.9547738693467, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI4501853, Length=407, Percent_Identity=40.2948402948403, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI4557237, Length=402, Percent_Identity=35.3233830845771, Blast_Score=214, Evalue=2e-55,
Organism=Homo sapiens, GI4504327, Length=434, Percent_Identity=31.7972350230415, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI194353979, Length=400, Percent_Identity=29.5, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=64.3391521197007, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI1788554, Length=404, Percent_Identity=44.8019801980198, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI87082165, Length=400, Percent_Identity=43.75, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI48994986, Length=408, Percent_Identity=42.4019607843137, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI1788683, Length=413, Percent_Identity=30.2663438256659, Blast_Score=151, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI133906874, Length=403, Percent_Identity=41.9354838709677, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI25147385, Length=404, Percent_Identity=36.6336633663366, Blast_Score=229, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI17535921, Length=406, Percent_Identity=32.7586206896552, Blast_Score=191, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI17551802, Length=432, Percent_Identity=31.4814814814815, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17535917, Length=402, Percent_Identity=30.8457711442786, Blast_Score=156, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6322031, Length=410, Percent_Identity=37.8048780487805, Blast_Score=239, Evalue=5e-64,
Organism=Saccharomyces cerevisiae, GI6325229, Length=408, Percent_Identity=35.7843137254902, Blast_Score=207, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24655093, Length=399, Percent_Identity=42.6065162907268, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI17648125, Length=407, Percent_Identity=39.5577395577396, Blast_Score=254, Evalue=5e-68,
Organism=Drosophila melanogaster, GI24640423, Length=402, Percent_Identity=33.8308457711443, Blast_Score=202, Evalue=4e-52,
Organism=Drosophila melanogaster, GI17137578, Length=428, Percent_Identity=32.0093457943925, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI19921506, Length=327, Percent_Identity=25.9938837920489, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24585051, Length=325, Percent_Identity=25.5384615384615, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41737; Mature: 41737

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDVFICDAVRTPIGRFGGALAKVRTDDLAATPLKALKAKHPNLDWAAVDEVFFGCANQA
CCCEEEEHHHHCCHHHHCCHHHHEECCCHHHCCHHHHHCCCCCCCHHHHHHHHHHHCCCC
GEDNRNVARMALLLAGLPDSIPGQTLNRLCASGLDAVGAAGRAIRAGEVDLAIAGGVESM
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHCCCCEEECCCEEEEEECCHHHH
TRAPFVQGKATEAFSRSAEIFDTTIGWRFINPLMKSQYGVDSMPETGENVAEEFQVSRAD
HHCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
QDAFAIRSQQRAGQAIASGYFAAEIAQVAVPGGKAGPIIVDKDEHPRPETTLEGLAKLKP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHH
IVRNPGTVTAGNASGVNDGAAAMILASEAAVKKHGLTPRARVLGLASAGVPPRIMGIGPV
HHCCCCEEECCCCCCCCCCCEEEEEEHHHHHHHCCCCHHHHHEEECCCCCCCEEEECCCH
PAVRKLLERLGLKITDFDLIELNEAFASQGIACLRQLGVAEDADFVNPHGGAIALGHPLG
HHHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCC
MSGARIAMTAVHGLEVRGGKRALATMCVGVGQGVALAVEMVN
CCCCEEEEEHHHCEEECCCHHHHHHHHHHCCCCEEEEEEECC
>Mature Secondary Structure
MRDVFICDAVRTPIGRFGGALAKVRTDDLAATPLKALKAKHPNLDWAAVDEVFFGCANQA
CCCEEEEHHHHCCHHHHCCHHHHEECCCHHHCCHHHHHCCCCCCCHHHHHHHHHHHCCCC
GEDNRNVARMALLLAGLPDSIPGQTLNRLCASGLDAVGAAGRAIRAGEVDLAIAGGVESM
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHCCCCEEECCCEEEEEECCHHHH
TRAPFVQGKATEAFSRSAEIFDTTIGWRFINPLMKSQYGVDSMPETGENVAEEFQVSRAD
HHCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
QDAFAIRSQQRAGQAIASGYFAAEIAQVAVPGGKAGPIIVDKDEHPRPETTLEGLAKLKP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHH
IVRNPGTVTAGNASGVNDGAAAMILASEAAVKKHGLTPRARVLGLASAGVPPRIMGIGPV
HHCCCCEEECCCCCCCCCCCEEEEEEHHHHHHHCCCCHHHHHEEECCCCCCCEEEECCCH
PAVRKLLERLGLKITDFDLIELNEAFASQGIACLRQLGVAEDADFVNPHGGAIALGHPLG
HHHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCC
MSGARIAMTAVHGLEVRGGKRALATMCVGVGQGVALAVEMVN
CCCCEEEEEHHHCEEECCCHHHHHHHHHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]