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The map label for this gene is braE [H]

Identifier: 146337631

GI number: 146337631

Start: 499555

End: 500625

Strand: Reverse

Name: braE [H]

Synonym: BRADO0496

Alternate gene names: 146337631

Gene position: 500625-499555 (Counterclockwise)

Preceding gene: 146337632

Following gene: 146337630

Centisome position: 6.71

GC content: 66.29

Gene sequence:

>1071_bases
ATGATGATCCTGTCCGGCGATCCGCCCCGCAGCCGCGCGCTGTCGATCCTGCTCGTCGTCATCGTGCTGGCGCTGCTGGC
GACGCCCTTCATCTTCCCCGGAGCCAAGGCGCTCAACGTCGCCGCCAAGATCTGCATCTTCGCGGCGCTGGTCGCCTCCT
ATGACCTGCTGCTCGGCTACACCGGGTCGGTGTCGTTCGCGCATACGATGTTCTATGGCATCGGCTCCTACGCGATCGCG
ATCGCGCTGTATGGACTCGGCCCCAATTGGACCTCCGTGGCGGTCGCGGTCGCCGCAGGGCTGCCGCTCGCCTTGCTGCT
GGCGCTCGCGATCGGGCTGTTCTCGCTGCGCGTCGAGGCGATCTTCTTCGCCATGATCACGCTCGCGATCGCCTCCGCCT
TCCTCGTGCTGGCCTCGCAGCTGTCGTGGTTGACGGGCGGCGAGGACGGCCGCAGCTTCCAGCTGCCCGAGCTGCTGCGC
CCCTCCACCGTGTTCATCGGCAAGGAGTGGTTCGGCTTTCCCATCAATGGCCGCATCCTGACCTATTATCTGGTGCTGTC
GGCCTGCGCCTTGATGATCCTGGCGCTGCTGCGTGTCGTGAACTCGCCGTTCGGCCGCGTGCTGCAGGCGATCCGCGAAA
ACCGCTTCCGCGCCGAGGCGCTGGGCTATCGCACCGTCTTCCATCTCACCTATGCCAACTGCATCGCGGCGGTGATCGCG
GCCTGCGCCGGCATGCTGAACGCGCTGTGGCTGCGCTATGCCGGGCCTGACACATCGCTGGCGTTCTCGATCATGCTCGA
CATTCTGCTGATGGTCGTGATCGGCGGCATGGGCACGATCTACGGCGCGATCATCGGCGCCGCGATCTTCATCCTGGCGC
AGAACTATCTCCAGGCGCTGATGGGTATCGCCTCGAGCGCCGCCGCGGACGCCGGGCTTCCGCTGCTGCCCGGACTTCTA
CACCCCGACCGCTGGCTGCTGTGGCTCGGTCTGCTGTTCATCGCCAGCGTCTATTTCTTTCCCACGGGGGTGGTCGGCCG
CCTTCGCAACACGCCTGGCAGCCCGCGCTGA

Upstream 100 bases:

>100_bases
GCGGCTTCCTGGTGCCCAAGCTCGCCCTCGTCTCCAACATCCTGCTGATGGTCGCCGTGCTGATGTGGCGGCCGCGCGGC
CTCTATGCGGTGACCAGCCG

Downstream 100 bases:

>100_bases
GCTGCAATTACCCTTAAAACTCTCCGCAATCTGGCGATCATCGCCGCCCTGATGCGGTACTCGAATGTAGAAATCACTGG
CTCCGACGACCTTTCGCGAT

Product: putative branched-chain amino acid ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MMILSGDPPRSRALSILLVVIVLALLATPFIFPGAKALNVAAKICIFAALVASYDLLLGYTGSVSFAHTMFYGIGSYAIA
IALYGLGPNWTSVAVAVAAGLPLALLLALAIGLFSLRVEAIFFAMITLAIASAFLVLASQLSWLTGGEDGRSFQLPELLR
PSTVFIGKEWFGFPINGRILTYYLVLSACALMILALLRVVNSPFGRVLQAIRENRFRAEALGYRTVFHLTYANCIAAVIA
ACAGMLNALWLRYAGPDTSLAFSIMLDILLMVVIGGMGTIYGAIIGAAIFILAQNYLQALMGIASSAAADAGLPLLPGLL
HPDRWLLWLGLLFIASVYFFPTGVVGRLRNTPGSPR

Sequences:

>Translated_356_residues
MMILSGDPPRSRALSILLVVIVLALLATPFIFPGAKALNVAAKICIFAALVASYDLLLGYTGSVSFAHTMFYGIGSYAIA
IALYGLGPNWTSVAVAVAAGLPLALLLALAIGLFSLRVEAIFFAMITLAIASAFLVLASQLSWLTGGEDGRSFQLPELLR
PSTVFIGKEWFGFPINGRILTYYLVLSACALMILALLRVVNSPFGRVLQAIRENRFRAEALGYRTVFHLTYANCIAAVIA
ACAGMLNALWLRYAGPDTSLAFSIMLDILLMVVIGGMGTIYGAIIGAAIFILAQNYLQALMGIASSAAADAGLPLLPGLL
HPDRWLLWLGLLFIASVYFFPTGVVGRLRNTPGSPR
>Mature_356_residues
MMILSGDPPRSRALSILLVVIVLALLATPFIFPGAKALNVAAKICIFAALVASYDLLLGYTGSVSFAHTMFYGIGSYAIA
IALYGLGPNWTSVAVAVAAGLPLALLLALAIGLFSLRVEAIFFAMITLAIASAFLVLASQLSWLTGGEDGRSFQLPELLR
PSTVFIGKEWFGFPINGRILTYYLVLSACALMILALLRVVNSPFGRVLQAIRENRFRAEALGYRTVFHLTYANCIAAVIA
ACAGMLNALWLRYAGPDTSLAFSIMLDILLMVVIGGMGTIYGAIIGAAIFILAQNYLQALMGIASSAAADAGLPLLPGLL
HPDRWLLWLGLLFIASVYFFPTGVVGRLRNTPGSPR

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=281, Percent_Identity=27.0462633451957, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 37924; Mature: 37924

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMILSGDPPRSRALSILLVVIVLALLATPFIFPGAKALNVAAKICIFAALVASYDLLLGY
CEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
TGSVSFAHTMFYGIGSYAIAIALYGLGPNWTSVAVAVAAGLPLALLLALAIGLFSLRVEA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
IFFAMITLAIASAFLVLASQLSWLTGGEDGRSFQLPELLRPSTVFIGKEWFGFPINGRIL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCEEEEECCCCCCCCCCHHH
TYYLVLSACALMILALLRVVNSPFGRVLQAIRENRFRAEALGYRTVFHLTYANCIAAVIA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ACAGMLNALWLRYAGPDTSLAFSIMLDILLMVVIGGMGTIYGAIIGAAIFILAQNYLQAL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
MGIASSAAADAGLPLLPGLLHPDRWLLWLGLLFIASVYFFPTGVVGRLRNTPGSPR
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MMILSGDPPRSRALSILLVVIVLALLATPFIFPGAKALNVAAKICIFAALVASYDLLLGY
CEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
TGSVSFAHTMFYGIGSYAIAIALYGLGPNWTSVAVAVAAGLPLALLLALAIGLFSLRVEA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
IFFAMITLAIASAFLVLASQLSWLTGGEDGRSFQLPELLRPSTVFIGKEWFGFPINGRIL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCEEEEECCCCCCCCCCHHH
TYYLVLSACALMILALLRVVNSPFGRVLQAIRENRFRAEALGYRTVFHLTYANCIAAVIA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ACAGMLNALWLRYAGPDTSLAFSIMLDILLMVVIGGMGTIYGAIIGAAIFILAQNYLQAL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
MGIASSAAADAGLPLLPGLLHPDRWLLWLGLLFIASVYFFPTGVVGRLRNTPGSPR
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]