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The map label for this gene is priA [H]

Identifier: 146337559

GI number: 146337559

Start: 422525

End: 424735

Strand: Direct

Name: priA [H]

Synonym: BRADO0413

Alternate gene names: 146337559

Gene position: 422525-424735 (Clockwise)

Preceding gene: 146337556

Following gene: 146337561

Centisome position: 5.67

GC content: 70.1

Gene sequence:

>2211_bases
ATGGACCGCCCGACCGATCTCTTTCCAGGTGCCACCAGCGCGGCCGGCGTGGTCGACGTGCTGGTTCCGGTCGCGCTCGA
TCACACCTATTCCTACCGCGTGCCGCGCGGCATGGCGCTGAAGCCGGGCGATGTGGTCGGTGTGCCGCTCGGCCCCCGCG
AGGTGCTGGCAGTCGTATGGGCCGAGAACGCCAATCCCGATCCGCGCCTGCACAACCGGCTCAAGGACGTCAGCGAAAAG
CTCGATGTGCCGCCGCTCAAGCCGGAGCTGCGGTCGCTGGTCGACTGGGTCGCCAATTACACGCTGTCGCCGCGCGGCAT
GGTGCTGCGGATGGCCCTGCGCATGGGCGAGCATCTCGGCCCCGAGCGGGTCCGGCTCGGCGTCCGCCTAGTCGGCGAGC
CCCCGAAGCGCCTGACGCCGGCGCGGCGGCGGGTGATCGAGGCGGTGTCGGACGGCTTGCTGCATGGCAAGTCCGATCTC
GTCCGCGAGATCGGCGTCTCTGCGAGCGTGATCGACGGCCTGGTCGACGAGGGGACGCTGACTGTCGAAGCCATGCCGCG
CGTGCTGCCGCCGCCGACGCCCGATCCGTCCTTCGCGCAGCCGGAGTTCTTCGATGCCCAGCTCGCCGGCGTCGAGGCCA
TGCGGGCTCTGGCGGCGTGCGGCACCTTCAACGTCGCGCTGCTCGACGGCGTCACCGGCTCCGGCAAGACCGAGGTGTAT
TTCGAGGCCGTGGCCGAGACGGTCCGGCGCGGCCGGCAGTCGCTGATCCTGATGCCGGAGATCGCGCTGACAGGGCAGTT
TCTCGAGCGCTTCTCGCGCCGCTTCGGCGTGCGTCCCCTGGAATGGCATTCCGAGCTGACGCCGCGTACCCGCCAGCGCA
ATTGGGCGGCGATTGCCGCCGGCGAGGCGCCGGTCGTGGTCGGCGCGCGCTCGGCGCTGTTCCTGCCCTATGCCGATCTT
GGTCTCATCGTCGTCGATGAGGAGCACGACCAGGCCTACAAGCAGGACGAGGGCGCGCATTACCACGCCCGCGACATGGC
GGTGGTGCGCGGCCATATCGCCAAGTTTCCGGTGGTGCTGGCCTCCGCGACGCCCTCGGTCGAGACCGAGGTCAATGCCC
GCAAGGGCCGCTACCAGCGCGTCGCGCTGCCGTCGCGCTTCGGCGGCCAGCACATGCCGCATATCGAGGCCATCGATCTC
CGCCGCGAGCCGCCGCCGCGCGGCCGCTACGTCTCGCCGCGGCTGGCCGGCCATATCCGCACCGCGATCGAGCGCGGCGA
GCAGGCGCTCTTGTTCCTGAACCGCCGCGGCTATGCGCCGCTGACCCTGTGCCGCGCCTGCGGCCACCGCTTCGCCTGCA
CCATCTGCGACGCCTGGCTGGTCGACCACCGATTTCGGCAGCGGTTGGTCTGTCATCACTGCGGGTTCTCGATGGCGCGG
CCGCCGACCTGTCCGCATTGCGCGGCCGAGGAGTCGCTGGTCGCCGTCGGTCCCGGCGTGGAGCGGCTGCAGGAGGAGGC
GGCGGCGCTGTTTCCGGACGCCCGCAGCATGGTGCTGTCGAGCGACCTGATCACCTCGATCGAGACCATGCGCGCGGAGC
TGACGGCGATCGCGGAGGGCCGCGTCGATCTCATCATCGGTACCCAGCTCGTGGCCAAGGGCCACAACTTCCCGCGGCTC
AATCTGGTCGGGGTGGTCGACGCCGATCTCGGCCTCGGGAATGGCGATCCGCGCGCCGCCGAGCGCACCTGGCAACTTCT
CAACCAGGTGATCGGTCGCGCCGGCCGCGAGCAGGGACGTGGCCGCGGCTATCTGCAGACCCACCAGCCCGAGCACCCCG
TGATGAAGGCGCTGGTCGCCTGCGACCGCGAGGCGTTCTACACCAGCGAGATCGAGGCGCGCGAACGCACCGGCTATCCG
CCGTTCGGCCGGCTGGCAAGCCTGATCATCTCGGCCGGCGACCGGCCGACAGCGGAAGGATTTGCGAGACGGCTGGTGGC
GTCAGCGCCGCGGGAAGAGGGCGTCCTGCTGCTCGGCCCGGCCGAGGCGCCGCTGGCCGTCATCAAGGGCCGCTACCGTT
TCCGGTTGCTGCTCAAGTCGGCGCGCAATTTCGATCTGTCGGATTACCTGCGCCACTGGCTGGCGCATGCCGAGAAGCCC
AAGGGCAACCTCAAGCTCGAGGTCGACGTCGACCCGCAGAGCTTCTTGTAG

Upstream 100 bases:

>100_bases
ATCTCATGACGATCCCATATATCGCATCCTCGTCCTTTACCGTTCGCTAACTTTGCCGGGCGGCTTTCGCTTCCATCCGA
GCAAGGCTATCTCCGCCCTC

Downstream 100 bases:

>100_bases
CGCAAGGCGGTCTCTCCGACGTCATTGCGAGCGCAGCGAAGCAATCCAGGGGCCGGGGTGGGACTCTGGATTGCTTCGTC
GCTTCGCTCCTCGCAATGAC

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 736; Mature: 736

Protein sequence:

>736_residues
MDRPTDLFPGATSAAGVVDVLVPVALDHTYSYRVPRGMALKPGDVVGVPLGPREVLAVVWAENANPDPRLHNRLKDVSEK
LDVPPLKPELRSLVDWVANYTLSPRGMVLRMALRMGEHLGPERVRLGVRLVGEPPKRLTPARRRVIEAVSDGLLHGKSDL
VREIGVSASVIDGLVDEGTLTVEAMPRVLPPPTPDPSFAQPEFFDAQLAGVEAMRALAACGTFNVALLDGVTGSGKTEVY
FEAVAETVRRGRQSLILMPEIALTGQFLERFSRRFGVRPLEWHSELTPRTRQRNWAAIAAGEAPVVVGARSALFLPYADL
GLIVVDEEHDQAYKQDEGAHYHARDMAVVRGHIAKFPVVLASATPSVETEVNARKGRYQRVALPSRFGGQHMPHIEAIDL
RREPPPRGRYVSPRLAGHIRTAIERGEQALLFLNRRGYAPLTLCRACGHRFACTICDAWLVDHRFRQRLVCHHCGFSMAR
PPTCPHCAAEESLVAVGPGVERLQEEAAALFPDARSMVLSSDLITSIETMRAELTAIAEGRVDLIIGTQLVAKGHNFPRL
NLVGVVDADLGLGNGDPRAAERTWQLLNQVIGRAGREQGRGRGYLQTHQPEHPVMKALVACDREAFYTSEIEARERTGYP
PFGRLASLIISAGDRPTAEGFARRLVASAPREEGVLLLGPAEAPLAVIKGRYRFRLLLKSARNFDLSDYLRHWLAHAEKP
KGNLKLEVDVDPQSFL

Sequences:

>Translated_736_residues
MDRPTDLFPGATSAAGVVDVLVPVALDHTYSYRVPRGMALKPGDVVGVPLGPREVLAVVWAENANPDPRLHNRLKDVSEK
LDVPPLKPELRSLVDWVANYTLSPRGMVLRMALRMGEHLGPERVRLGVRLVGEPPKRLTPARRRVIEAVSDGLLHGKSDL
VREIGVSASVIDGLVDEGTLTVEAMPRVLPPPTPDPSFAQPEFFDAQLAGVEAMRALAACGTFNVALLDGVTGSGKTEVY
FEAVAETVRRGRQSLILMPEIALTGQFLERFSRRFGVRPLEWHSELTPRTRQRNWAAIAAGEAPVVVGARSALFLPYADL
GLIVVDEEHDQAYKQDEGAHYHARDMAVVRGHIAKFPVVLASATPSVETEVNARKGRYQRVALPSRFGGQHMPHIEAIDL
RREPPPRGRYVSPRLAGHIRTAIERGEQALLFLNRRGYAPLTLCRACGHRFACTICDAWLVDHRFRQRLVCHHCGFSMAR
PPTCPHCAAEESLVAVGPGVERLQEEAAALFPDARSMVLSSDLITSIETMRAELTAIAEGRVDLIIGTQLVAKGHNFPRL
NLVGVVDADLGLGNGDPRAAERTWQLLNQVIGRAGREQGRGRGYLQTHQPEHPVMKALVACDREAFYTSEIEARERTGYP
PFGRLASLIISAGDRPTAEGFARRLVASAPREEGVLLLGPAEAPLAVIKGRYRFRLLLKSARNFDLSDYLRHWLAHAEKP
KGNLKLEVDVDPQSFL
>Mature_736_residues
MDRPTDLFPGATSAAGVVDVLVPVALDHTYSYRVPRGMALKPGDVVGVPLGPREVLAVVWAENANPDPRLHNRLKDVSEK
LDVPPLKPELRSLVDWVANYTLSPRGMVLRMALRMGEHLGPERVRLGVRLVGEPPKRLTPARRRVIEAVSDGLLHGKSDL
VREIGVSASVIDGLVDEGTLTVEAMPRVLPPPTPDPSFAQPEFFDAQLAGVEAMRALAACGTFNVALLDGVTGSGKTEVY
FEAVAETVRRGRQSLILMPEIALTGQFLERFSRRFGVRPLEWHSELTPRTRQRNWAAIAAGEAPVVVGARSALFLPYADL
GLIVVDEEHDQAYKQDEGAHYHARDMAVVRGHIAKFPVVLASATPSVETEVNARKGRYQRVALPSRFGGQHMPHIEAIDL
RREPPPRGRYVSPRLAGHIRTAIERGEQALLFLNRRGYAPLTLCRACGHRFACTICDAWLVDHRFRQRLVCHHCGFSMAR
PPTCPHCAAEESLVAVGPGVERLQEEAAALFPDARSMVLSSDLITSIETMRAELTAIAEGRVDLIIGTQLVAKGHNFPRL
NLVGVVDADLGLGNGDPRAAERTWQLLNQVIGRAGREQGRGRGYLQTHQPEHPVMKALVACDREAFYTSEIEARERTGYP
PFGRLASLIISAGDRPTAEGFARRLVASAPREEGVLLLGPAEAPLAVIKGRYRFRLLLKSARNFDLSDYLRHWLAHAEKP
KGNLKLEVDVDPQSFL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=759, Percent_Identity=33.596837944664, Blast_Score=360, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD [H]

EC number: NA

Molecular weight: Translated: 80933; Mature: 80933

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRPTDLFPGATSAAGVVDVLVPVALDHTYSYRVPRGMALKPGDVVGVPLGPREVLAVVW
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCEECCCCEEECCCCHHHEEEEEE
AENANPDPRLHNRLKDVSEKLDVPPLKPELRSLVDWVANYTLSPRGMVLRMALRMGEHLG
ECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
PERVRLGVRLVGEPPKRLTPARRRVIEAVSDGLLHGKSDLVREIGVSASVIDGLVDEGTL
HHHHEEEEEEECCCCHHCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHCCCEE
TVEAMPRVLPPPTPDPSFAQPEFFDAQLAGVEAMRALAACGTFNVALLDGVTGSGKTEVY
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHH
FEAVAETVRRGRQSLILMPEIALTGQFLERFSRRFGVRPLEWHSELTPRTRQRNWAAIAA
HHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHCCCEEEEEC
GEAPVVVGARSALFLPYADLGLIVVDEEHDQAYKQDEGAHYHARDMAVVRGHIAKFPVVL
CCCCEEEECCCEEEECCCCCCEEEEECCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCEEE
ASATPSVETEVNARKGRYQRVALPSRFGGQHMPHIEAIDLRREPPPRGRYVSPRLAGHIR
ECCCCCCCCCCCCCCCCEEEEECCHHCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHH
TAIERGEQALLFLNRRGYAPLTLCRACGHRFACTICDAWLVDHRFRQRLVCHHCGFSMAR
HHHHCCCEEEEEEECCCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PPTCPHCAAEESLVAVGPGVERLQEEAAALFPDARSMVLSSDLITSIETMRAELTAIAEG
CCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
RVDLIIGTQLVAKGHNFPRLNLVGVVDADLGLGNGDPRAAERTWQLLNQVIGRAGREQGR
CEEEEECCHHHHCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHCCC
GRGYLQTHQPEHPVMKALVACDREAFYTSEIEARERTGYPPFGRLASLIISAGDRPTAEG
CCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHH
FARRLVASAPREEGVLLLGPAEAPLAVIKGRYRFRLLLKSARNFDLSDYLRHWLAHAEKP
HHHHHHHCCCCCCCEEEECCCCCCHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
KGNLKLEVDVDPQSFL
CCCEEEEEECCHHHCC
>Mature Secondary Structure
MDRPTDLFPGATSAAGVVDVLVPVALDHTYSYRVPRGMALKPGDVVGVPLGPREVLAVVW
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCEECCCCEEECCCCHHHEEEEEE
AENANPDPRLHNRLKDVSEKLDVPPLKPELRSLVDWVANYTLSPRGMVLRMALRMGEHLG
ECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
PERVRLGVRLVGEPPKRLTPARRRVIEAVSDGLLHGKSDLVREIGVSASVIDGLVDEGTL
HHHHEEEEEEECCCCHHCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHCCCEE
TVEAMPRVLPPPTPDPSFAQPEFFDAQLAGVEAMRALAACGTFNVALLDGVTGSGKTEVY
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHH
FEAVAETVRRGRQSLILMPEIALTGQFLERFSRRFGVRPLEWHSELTPRTRQRNWAAIAA
HHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHCCCEEEEEC
GEAPVVVGARSALFLPYADLGLIVVDEEHDQAYKQDEGAHYHARDMAVVRGHIAKFPVVL
CCCCEEEECCCEEEECCCCCCEEEEECCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCEEE
ASATPSVETEVNARKGRYQRVALPSRFGGQHMPHIEAIDLRREPPPRGRYVSPRLAGHIR
ECCCCCCCCCCCCCCCCEEEEECCHHCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHH
TAIERGEQALLFLNRRGYAPLTLCRACGHRFACTICDAWLVDHRFRQRLVCHHCGFSMAR
HHHHCCCEEEEEEECCCCCHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PPTCPHCAAEESLVAVGPGVERLQEEAAALFPDARSMVLSSDLITSIETMRAELTAIAEG
CCCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
RVDLIIGTQLVAKGHNFPRLNLVGVVDADLGLGNGDPRAAERTWQLLNQVIGRAGREQGR
CEEEEECCHHHHCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHCCC
GRGYLQTHQPEHPVMKALVACDREAFYTSEIEARERTGYPPFGRLASLIISAGDRPTAEG
CCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHH
FARRLVASAPREEGVLLLGPAEAPLAVIKGRYRFRLLLKSARNFDLSDYLRHWLAHAEKP
HHHHHHHCCCCCCCEEEECCCCCCHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
KGNLKLEVDVDPQSFL
CCCEEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2861810 [H]