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The map label for this gene is gacS [H]

Identifier: 146337386

GI number: 146337386

Start: 230205

End: 232469

Strand: Reverse

Name: gacS [H]

Synonym: BRADO0219

Alternate gene names: 146337386

Gene position: 232469-230205 (Counterclockwise)

Preceding gene: 146337387

Following gene: 146337378

Centisome position: 3.12

GC content: 68.52

Gene sequence:

>2265_bases
ATGGGACAGGGACTTCGCATCAGGTTGCATCTGCGCCGGTTCATGCGCCGTCATCAGCTCGTCTTCACCATCCTGCGCTC
GCTGATGATCTTCTCCGCCGGTTTCGGCGGCGCCTACGGCTTCATCAAGGCCAGCATCTCCGAGCACTCCGGCTACAACC
CGCATGCCTTCGCGATCGGCGCCAGCTTCCTGTTCGCGCTGGCCTGCGTCGGCCTCGCCACGCTCAGCATGCGGCTGCGC
TTCGTCCATAAGAAGCTGCACAAGATCGCGCTCCACAACGAGGTGCTGGCGGACCGGAACTGGGAGCTGCAGGAAGCCGA
GCAGCGCGCGCGCAATCTGTTCGAGCAGCAGGGCGATCTCATCGTCATGCGTGAGCAGGACGGCACCATCGTGTTCGTCA
ACGACGCCTATTGCCAGATGGCAGGCCTCGATCGCGAGAACCTGGTCGGCAGCAAATTCGCCTTCGCACTGATCGAGCAG
GGCGACAGCGCCCTGGAGCCGAGCGGCACGCGCGTCCACGACCAGCGCATCGAGAGCGCGATCGGGCCGCGCTGGATCGC
CTGGCGCGAGGCGCTGGTGCGCTCCGATGCCGGCCACCCGGCCGTGCTGCAATGTGTCGGCCGCGACGTGACCGACCGCA
CCGAGACCGAGCGGGCGCTGGCGGATGCGCGCGACCAGGCCAATGCGGCCAATCGGGCCAAGTCGCGCTTCCTGGCGATG
GCCAGCCACGAGATCCGCACGCCGCTGAACGGAATCCTTGGCATGGGCGGGCTGCTGCTCGAGACGCCGCTGACGCCGGA
GCAGACGACCTACGCCAAGGCGGTGCGCGCATCAGGCGAGGCGCTGATGTCGCTGATCAACGAGCTGCTCGATTATTCCA
AGATCGAGGCCGGCAAGCTGGCGCTGGAGGCGCAGCCGTTCTCGCTTGCCGCGCTAATCGAGGAGATCACCGAGCTGCTC
GCGCCGCGCGCGCAAGCCAAGCAGCTGGAAATCGCGGCCTACATGGACGAGCGGCTGCCGACCTGGGTGACCGGCGACGC
CGCGCGCCTGCGCCAGGTGCTGCTCAATCTCGCCGGCAACGCCATCAAGTTCACGGCCAAGGGCGGCGTCGCTGTCATTG
CCGAGCCCGGCATCTGGCCGAACGAGATCAGTTTTCAGGTGCGCGACACCGGCATCGGCATCGCCGCCGAGGCGCAGGAG
CGGATCTTCCGCGAGTTCGAGCAGGCCGACGACAGCGTCGCGCGAAGTTTTGGCGGCACCGGGCTTGGGCTCGCGATCTC
CGACCGCATCGTCAAGCGCATGGGCGGCCGCATCAGCCTGCAGAGCGAGCCCGGCGCCGGCGCGACCTTCGAGGTCTGCG
TGCCGCTGCCCGCGGCGCTGGAGCGCGGCGAGCAGAACAGCTTTCGCGGGCCCGATCTGGCGGGCCAATCGATCATGCTG
ATCGCGCCCGCCGGGCTGCAGGCGGAGCTGATCGAGCGCCGTCTGCAGAACTGGGGCGCGCAGACGAGTCTCGTCACCGA
CACCGGGGTCGCGGAGGCGCTGCTGCCCGAGCGCACGTGGCATGCCGTTCTGGTCGATGATGCCGTCGGCGGCGAGCGGG
CGCGACATCTCAGCGAGGCCGCGCGGCGGCATACGACGCGGCTGATCGTGCTGGTCACGCCGGTCAGCCGGCACGAGCTG
ATGCCGGTGGCGCCGTTCACCGGCTATCTGGTCAAGCCACTGCGCGCCGCGTCGCTCGCCGCGCGCGTCGGCTTCGATGT
CGATGGCGATGGCGATGGCGATGGCGATGGTCCTGCCGGGGCCGAGATCGAAGAGTCTGTCCCGATGGCCGCGCCGCCGA
TCGCAGCGCGAGCCGGCAGCGGCCTGTCGATCCTGGTCGCCGAAGACAACGACATCAACGCGCTCTTAATGCGCTCGCTG
CTGACCAAGCTCGGCCATCATGCGGATATCGCCGTCCACGGCGAGGCCGCACTCGACTCCTGGCTCGCGGCGCGCTCGGC
TGGCACGCCCTACGACCTGATCCTGATGGACGTCCAGATGCCACGCCTCGACGGCATCGCCGCTGCCAAGCGTATCCGCG
CACTGGAGGCGACCGAGGCCGGCCGGCGGACGCCGATCCTGGCCTTGACCGCAAACACCCTGGTCGAGGACCGCTACGCC
TGTTTTGAAGCCGGTATGGACGGCTTCCTGGTCAAGCCGATCGACCGCGACAAGCTGGACGAGGCGCTGGCCAATCTGGC
GGCGGCACGGCAGGTGGCGGCTTAG

Upstream 100 bases:

>100_bases
AGGACGCGCCTGCATGAGCTTTGAAAGTCTCCGCAAGACCCGGCGCGGCATGATGCGCGGCGGCCAGGACATTGGGAATC
GCAATCTCTGGGGAGGAACG

Downstream 100 bases:

>100_bases
TTATGAGTTTGTAGTACGCAACGTGTTTGCGCTCCCTCTCCCCGTTCTTCACGGGGAGAGGGTCGGGGTGAGGGGCAGCC
ACACGGGTGGTGCCAACTCT

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 754; Mature: 753

Protein sequence:

>754_residues
MGQGLRIRLHLRRFMRRHQLVFTILRSLMIFSAGFGGAYGFIKASISEHSGYNPHAFAIGASFLFALACVGLATLSMRLR
FVHKKLHKIALHNEVLADRNWELQEAEQRARNLFEQQGDLIVMREQDGTIVFVNDAYCQMAGLDRENLVGSKFAFALIEQ
GDSALEPSGTRVHDQRIESAIGPRWIAWREALVRSDAGHPAVLQCVGRDVTDRTETERALADARDQANAANRAKSRFLAM
ASHEIRTPLNGILGMGGLLLETPLTPEQTTYAKAVRASGEALMSLINELLDYSKIEAGKLALEAQPFSLAALIEEITELL
APRAQAKQLEIAAYMDERLPTWVTGDAARLRQVLLNLAGNAIKFTAKGGVAVIAEPGIWPNEISFQVRDTGIGIAAEAQE
RIFREFEQADDSVARSFGGTGLGLAISDRIVKRMGGRISLQSEPGAGATFEVCVPLPAALERGEQNSFRGPDLAGQSIML
IAPAGLQAELIERRLQNWGAQTSLVTDTGVAEALLPERTWHAVLVDDAVGGERARHLSEAARRHTTRLIVLVTPVSRHEL
MPVAPFTGYLVKPLRAASLAARVGFDVDGDGDGDGDGPAGAEIEESVPMAAPPIAARAGSGLSILVAEDNDINALLMRSL
LTKLGHHADIAVHGEAALDSWLAARSAGTPYDLILMDVQMPRLDGIAAAKRIRALEATEAGRRTPILALTANTLVEDRYA
CFEAGMDGFLVKPIDRDKLDEALANLAAARQVAA

Sequences:

>Translated_754_residues
MGQGLRIRLHLRRFMRRHQLVFTILRSLMIFSAGFGGAYGFIKASISEHSGYNPHAFAIGASFLFALACVGLATLSMRLR
FVHKKLHKIALHNEVLADRNWELQEAEQRARNLFEQQGDLIVMREQDGTIVFVNDAYCQMAGLDRENLVGSKFAFALIEQ
GDSALEPSGTRVHDQRIESAIGPRWIAWREALVRSDAGHPAVLQCVGRDVTDRTETERALADARDQANAANRAKSRFLAM
ASHEIRTPLNGILGMGGLLLETPLTPEQTTYAKAVRASGEALMSLINELLDYSKIEAGKLALEAQPFSLAALIEEITELL
APRAQAKQLEIAAYMDERLPTWVTGDAARLRQVLLNLAGNAIKFTAKGGVAVIAEPGIWPNEISFQVRDTGIGIAAEAQE
RIFREFEQADDSVARSFGGTGLGLAISDRIVKRMGGRISLQSEPGAGATFEVCVPLPAALERGEQNSFRGPDLAGQSIML
IAPAGLQAELIERRLQNWGAQTSLVTDTGVAEALLPERTWHAVLVDDAVGGERARHLSEAARRHTTRLIVLVTPVSRHEL
MPVAPFTGYLVKPLRAASLAARVGFDVDGDGDGDGDGPAGAEIEESVPMAAPPIAARAGSGLSILVAEDNDINALLMRSL
LTKLGHHADIAVHGEAALDSWLAARSAGTPYDLILMDVQMPRLDGIAAAKRIRALEATEAGRRTPILALTANTLVEDRYA
CFEAGMDGFLVKPIDRDKLDEALANLAAARQVAA
>Mature_753_residues
GQGLRIRLHLRRFMRRHQLVFTILRSLMIFSAGFGGAYGFIKASISEHSGYNPHAFAIGASFLFALACVGLATLSMRLRF
VHKKLHKIALHNEVLADRNWELQEAEQRARNLFEQQGDLIVMREQDGTIVFVNDAYCQMAGLDRENLVGSKFAFALIEQG
DSALEPSGTRVHDQRIESAIGPRWIAWREALVRSDAGHPAVLQCVGRDVTDRTETERALADARDQANAANRAKSRFLAMA
SHEIRTPLNGILGMGGLLLETPLTPEQTTYAKAVRASGEALMSLINELLDYSKIEAGKLALEAQPFSLAALIEEITELLA
PRAQAKQLEIAAYMDERLPTWVTGDAARLRQVLLNLAGNAIKFTAKGGVAVIAEPGIWPNEISFQVRDTGIGIAAEAQER
IFREFEQADDSVARSFGGTGLGLAISDRIVKRMGGRISLQSEPGAGATFEVCVPLPAALERGEQNSFRGPDLAGQSIMLI
APAGLQAELIERRLQNWGAQTSLVTDTGVAEALLPERTWHAVLVDDAVGGERARHLSEAARRHTTRLIVLVTPVSRHELM
PVAPFTGYLVKPLRAASLAARVGFDVDGDGDGDGDGPAGAEIEESVPMAAPPIAARAGSGLSILVAEDNDINALLMRSLL
TKLGHHADIAVHGEAALDSWLAARSAGTPYDLILMDVQMPRLDGIAAAKRIRALEATEAGRRTPILALTANTLVEDRYAC
FEAGMDGFLVKPIDRDKLDEALANLAAARQVAA

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=549, Percent_Identity=32.9690346083789, Blast_Score=270, Evalue=3e-73,
Organism=Escherichia coli, GI145693157, Length=559, Percent_Identity=32.3792486583184, Blast_Score=239, Evalue=5e-64,
Organism=Escherichia coli, GI48994928, Length=503, Percent_Identity=29.2246520874751, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI87081816, Length=324, Percent_Identity=35.4938271604938, Blast_Score=160, Evalue=3e-40,
Organism=Escherichia coli, GI1788713, Length=285, Percent_Identity=34.3859649122807, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1788549, Length=366, Percent_Identity=26.775956284153, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786600, Length=382, Percent_Identity=26.9633507853403, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1790436, Length=218, Percent_Identity=32.1100917431193, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI87082128, Length=259, Percent_Identity=28.957528957529, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1788393, Length=228, Percent_Identity=29.3859649122807, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1786912, Length=258, Percent_Identity=27.5193798449612, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1789808, Length=299, Percent_Identity=29.0969899665552, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1790346, Length=228, Percent_Identity=28.5087719298246, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787894, Length=244, Percent_Identity=29.0983606557377, Blast_Score=71, Evalue=3e-13,
Organism=Escherichia coli, GI1786783, Length=225, Percent_Identity=25.7777777777778, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322044, Length=133, Percent_Identity=39.0977443609023, Blast_Score=95, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6322000, Length=123, Percent_Identity=32.520325203252, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 81489; Mature: 81358

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS50112 PAS ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQGLRIRLHLRRFMRRHQLVFTILRSLMIFSAGFGGAYGFIKASISEHSGYNPHAFAIG
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEEHHHCCCCCCHHHHHH
ASFLFALACVGLATLSMRLRFVHKKLHKIALHNEVLADRNWELQEAEQRARNLFEQQGDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCE
IVMREQDGTIVFVNDAYCQMAGLDRENLVGSKFAFALIEQGDSALEPSGTRVHDQRIESA
EEEEECCCEEEEEECCHHHHCCCCHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
IGPRWIAWREALVRSDAGHPAVLQCVGRDVTDRTETERALADARDQANAANRAKSRFLAM
CCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASHEIRTPLNGILGMGGLLLETPLTPEQTTYAKAVRASGEALMSLINELLDYSKIEAGKL
HHHHHHCCHHHHHCCCCCEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCE
ALEAQPFSLAALIEEITELLAPRAQAKQLEIAAYMDERLPTWVTGDAARLRQVLLNLAGN
EEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHCCC
AIKFTAKGGVAVIAEPGIWPNEISFQVRDTGIGIAAEAQERIFREFEQADDSVARSFGGT
EEEEEECCCEEEEECCCCCCCCEEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHCCCC
GLGLAISDRIVKRMGGRISLQSEPGAGATFEVCVPLPAALERGEQNSFRGPDLAGQSIML
CCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCHHHHHCCCCCCCCCCCCCCCEEEE
IAPAGLQAELIERRLQNWGAQTSLVTDTGVAEALLPERTWHAVLVDDAVGGERARHLSEA
EECCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHCCCCCEEEEEEECCCCCHHHHHHHHH
ARRHTTRLIVLVTPVSRHELMPVAPFTGYLVKPLRAASLAARVGFDVDGDGDGDGDGPAG
HHHHCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
AEIEESVPMAAPPIAARAGSGLSILVAEDNDINALLMRSLLTKLGHHADIAVHGEAALDS
CCHHHCCCCCCCCHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHH
WLAARSAGTPYDLILMDVQMPRLDGIAAAKRIRALEATEAGRRTPILALTANTLVEDRYA
HHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHH
CFEAGMDGFLVKPIDRDKLDEALANLAAARQVAA
HHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GQGLRIRLHLRRFMRRHQLVFTILRSLMIFSAGFGGAYGFIKASISEHSGYNPHAFAIG
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEEHHHCCCCCCHHHHHH
ASFLFALACVGLATLSMRLRFVHKKLHKIALHNEVLADRNWELQEAEQRARNLFEQQGDL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCE
IVMREQDGTIVFVNDAYCQMAGLDRENLVGSKFAFALIEQGDSALEPSGTRVHDQRIESA
EEEEECCCEEEEEECCHHHHCCCCHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
IGPRWIAWREALVRSDAGHPAVLQCVGRDVTDRTETERALADARDQANAANRAKSRFLAM
CCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASHEIRTPLNGILGMGGLLLETPLTPEQTTYAKAVRASGEALMSLINELLDYSKIEAGKL
HHHHHHCCHHHHHCCCCCEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCE
ALEAQPFSLAALIEEITELLAPRAQAKQLEIAAYMDERLPTWVTGDAARLRQVLLNLAGN
EEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHCCC
AIKFTAKGGVAVIAEPGIWPNEISFQVRDTGIGIAAEAQERIFREFEQADDSVARSFGGT
EEEEEECCCEEEEECCCCCCCCEEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHCCCC
GLGLAISDRIVKRMGGRISLQSEPGAGATFEVCVPLPAALERGEQNSFRGPDLAGQSIML
CCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCHHHHHCCCCCCCCCCCCCCCEEEE
IAPAGLQAELIERRLQNWGAQTSLVTDTGVAEALLPERTWHAVLVDDAVGGERARHLSEA
EECCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHCCCCCEEEEEEECCCCCHHHHHHHHH
ARRHTTRLIVLVTPVSRHELMPVAPFTGYLVKPLRAASLAARVGFDVDGDGDGDGDGPAG
HHHHCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
AEIEESVPMAAPPIAARAGSGLSILVAEDNDINALLMRSLLTKLGHHADIAVHGEAALDS
CCHHHCCCCCCCCHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHH
WLAARSAGTPYDLILMDVQMPRLDGIAAAKRIRALEATEAGRRTPILALTANTLVEDRYA
HHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHH
CFEAGMDGFLVKPIDRDKLDEALANLAAARQVAA
HHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]