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The map label for this gene is aacA4 [H]

Identifier: 146337376

GI number: 146337376

Start: 223576

End: 224088

Strand: Reverse

Name: aacA4 [H]

Synonym: BRADO0209

Alternate gene names: 146337376

Gene position: 224088-223576 (Counterclockwise)

Preceding gene: 146337377

Following gene: 146337375

Centisome position: 3.01

GC content: 64.52

Gene sequence:

>513_bases
ATGGTTGTTGGCTTTCGTGCGATGACCGAGGACGATCTGCCCGCGATCCGTGACTGGCTGCATCAGCCCCATGTGCGCGA
ATGGTGGGGCGATCCGGACGAGCAATATGCTCTGATCGATGGCGACCGTGATGAACACGCCATGGACCAGTACATCATGC
TGATAAACGAGGGCGCGGCCGGTTACCTGCAATGCTACGCCCTCACCCAATGGAACGACGGCTTCTTGCCGCAGCCGGAC
GGGACCCGCGGCATCGACCTGTTCATCGCTGGCCAGCAGCGGATCGGCCAGGGTCACGGTCCCCTGGTCATCCGGACCTT
TTGTAACCTGATGTTTCAGCGCGGCGTCCCGCGCGTGGTCACCGACCCCTCGCCGGCCAATGCCCGGGCCATCCGCGCCT
ATGAAAAGGCGGGATTCCGGCGCGAGCGCCTGGTCGACACGCCCGATGGACAGGCGCTGCTGATGTCCTGCGAACCCGCC
GACTTGCCTCGCCGCCCATCCGAGCCTAGCTAG

Upstream 100 bases:

>100_bases
CGCGACAACCTCACGCTCAACATCCTGATGCTGATCTATCCGACCGAAGCGATCCGGCAGTGGCAGGCCGGACCGCCGCT
GCTGTGAGGGTAGGGCGGTC

Downstream 100 bases:

>100_bases
AGCCCATGACCGACTTTTCTCCTCGCGAAATCGTCTCCGAACTCGACCGCTTCATCGTCGGCCAGGCCGACGCCAAGCGC
GCGGTCTCCATTGCGCTCCG

Product: putative aminoglycoside N(6')-acetyltransferase (aacA4)

Products: NA

Alternate protein names: AAC(6'); Amikacin resistance protein [H]

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MVVGFRAMTEDDLPAIRDWLHQPHVREWWGDPDEQYALIDGDRDEHAMDQYIMLINEGAAGYLQCYALTQWNDGFLPQPD
GTRGIDLFIAGQQRIGQGHGPLVIRTFCNLMFQRGVPRVVTDPSPANARAIRAYEKAGFRRERLVDTPDGQALLMSCEPA
DLPRRPSEPS

Sequences:

>Translated_170_residues
MVVGFRAMTEDDLPAIRDWLHQPHVREWWGDPDEQYALIDGDRDEHAMDQYIMLINEGAAGYLQCYALTQWNDGFLPQPD
GTRGIDLFIAGQQRIGQGHGPLVIRTFCNLMFQRGVPRVVTDPSPANARAIRAYEKAGFRRERLVDTPDGQALLMSCEPA
DLPRRPSEPS
>Mature_170_residues
MVVGFRAMTEDDLPAIRDWLHQPHVREWWGDPDEQYALIDGDRDEHAMDQYIMLINEGAAGYLQCYALTQWNDGFLPQPD
GTRGIDLFIAGQQRIGQGHGPLVIRTFCNLMFQRGVPRVVTDPSPANARAIRAYEKAGFRRERLVDTPDGQALLMSCEPA
DLPRRPSEPS

Specific function: Resistance to antibiotics containing the purpurosamine ring including gentamicin, kanamycin, tobramycin, and neomycin [H]

COG id: COG1670

COG function: function code J; Acetyltransferases, including N-acetylases of ribosomal proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR019432 [H]

Pfam domain/function: PF00583 Acetyltransf_1; PF10331 AlcB [H]

EC number: =2.3.1.82 [H]

Molecular weight: Translated: 19236; Mature: 19236

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVGFRAMTEDDLPAIRDWLHQPHVREWWGDPDEQYALIDGDRDEHAMDQYIMLINEGAA
CEEECCCCCCCCCHHHHHHHCCCCCHHHCCCCCCCEEEEECCCCHHHHHHEEEEEECCCC
GYLQCYALTQWNDGFLPQPDGTRGIDLFIAGQQRIGQGHGPLVIRTFCNLMFQRGVPRVV
CEEEEEEEECCCCCCCCCCCCCCCEEEEEECHHHCCCCCCCCHHHHHHHHHHHCCCCCEE
TDPSPANARAIRAYEKAGFRRERLVDTPDGQALLMSCEPADLPRRPSEPS
ECCCCCCHHHHHHHHHCCCCHHHCCCCCCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MVVGFRAMTEDDLPAIRDWLHQPHVREWWGDPDEQYALIDGDRDEHAMDQYIMLINEGAA
CEEECCCCCCCCCHHHHHHHCCCCCHHHCCCCCCCEEEEECCCCHHHHHHEEEEEECCCC
GYLQCYALTQWNDGFLPQPDGTRGIDLFIAGQQRIGQGHGPLVIRTFCNLMFQRGVPRVV
CEEEEEEEECCCCCCCCCCCCCCCEEEEEECHHHCCCCCCCCHHHHHHHHHHHCCCCCEE
TDPSPANARAIRAYEKAGFRRERLVDTPDGQALLMSCEPADLPRRPSEPS
ECCCCCCHHHHHHHHHCCCCHHHCCCCCCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2841303; 1963948 [H]