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The map label for this gene is hslU [H]

Identifier: 146337375

GI number: 146337375

Start: 222266

End: 223570

Strand: Reverse

Name: hslU [H]

Synonym: BRADO0208

Alternate gene names: 146337375

Gene position: 223570-222266 (Counterclockwise)

Preceding gene: 146337376

Following gene: 146337374

Centisome position: 3.0

GC content: 66.21

Gene sequence:

>1305_bases
ATGACCGACTTTTCTCCTCGCGAAATCGTCTCCGAACTCGACCGCTTCATCGTCGGCCAGGCCGACGCCAAGCGCGCGGT
CTCCATTGCGCTCCGCAACCGCTGGCGGCGGCTGCAGCTCGATACGGGCCTGCGCGAGGAGGTGCTGCCGAAGAACATCC
TGATGATCGGGCCGACCGGCGTCGGCAAGACCGAGATCGCGCGGCGGCTGGCCAAGCTCGCGGGCGCGCCGTTCCTGAAA
GTCGAGGCGACCAAATTCACCGAGGTCGGCTATGTCGGCCGCGACGTCGAGCAGATCATCCGCGACCTCGTCGAGGTCGC
GATCCATCAGGTGCGCGAGCGCAAGCGCAAGGACGTCGCCGCCCGCGCCCAGCTCGCCGCCGAGGAGCGGGTGCTCGATG
CGCTCGTCGGCGCCAATTCGAGTGCTGCGACCCGCGACTCGTTCCGCCGCAAGCTTCGCGCCGGCGAGCTCAACGACAAG
GAAATCGAGATCGAGACACAGTCCTCCGGCGGCGGCATGCCGATGTTCGAGATTCCGGGCATGCCGGGCGCGCAGATGGG
CGCGATCTCGATCGGCGACATCTTCGGCAAGCTCGGCGGCCGCACCAAGACGCGGCGCGTCACGGTCGAAGCCTCGCATG
ACATCCTGATCGCCGAGGAATCCGACAAGCTGCTCGACAGCGACCAGCTGGTGCAGGAGGCGATCAACGTGGTCGAGAAC
AACGGCATCGTGTTCCTCGACGAGATCGACAAGATCTGCGTGCGCGAGAACCGCCATGGCGGCGACGTGTCGCGCGAGGG
CGTGCAGCGCGATCTGCTGCCGCTGATCGAGGGCACGACGGTCTCGACCAAGCATGGCGCGGTGAAGACCGACCACATCC
TGTTCATCGCGTCCGGCGCGTTTCATATCGCGAAGCCCTCGGACCTGCTGCCCGAGTTGCAGGGCCGCCTGCCGATCCGC
GTCGAGCTGCAGGCCCTGACCCGCGACGACATGCGCCGCATCCTGACCGAGCCGGAGGCGTCGCTGATCAAGCAATATGT
GGCGCTGATGAAGACCGAGGGCGTGACGCTCGACATCACCGATGACGCGATCGATGCGCTCGCCGACGTCGCGGTGGCCG
TGAACTCCACGGTCGAGAACATCGGCGCTCGCCGGCTGCAGACGGTGATGGAGCGCGTGCTCGACGACATCTCGTTCACG
GCCTCCGATCGCACCGGCGAGACCATGCGCGTCGACGCCGCCTATGTGCAGCAGCACATCGGCGATCTCGCGAAGAATGC
AGATCTGAGCCGGTTCATTTTGTAG

Upstream 100 bases:

>100_bases
GGCGCGAGCGCCTGGTCGACACGCCCGATGGACAGGCGCTGCTGATGTCCTGCGAACCCGCCGACTTGCCTCGCCGCCCA
TCCGAGCCTAGCTAGAGCCC

Downstream 100 bases:

>100_bases
CCGCTGCTCAGATGAGCCGAGGCTTCGCCACGCATTCCGCCGTCGTCCCGGCGAACGCCGGGATCTATAACCACCGCAGC
TGATTGTTACGCACGATGGC

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MTDFSPREIVSELDRFIVGQADAKRAVSIALRNRWRRLQLDTGLREEVLPKNILMIGPTGVGKTEIARRLAKLAGAPFLK
VEATKFTEVGYVGRDVEQIIRDLVEVAIHQVRERKRKDVAARAQLAAEERVLDALVGANSSAATRDSFRRKLRAGELNDK
EIEIETQSSGGGMPMFEIPGMPGAQMGAISIGDIFGKLGGRTKTRRVTVEASHDILIAEESDKLLDSDQLVQEAINVVEN
NGIVFLDEIDKICVRENRHGGDVSREGVQRDLLPLIEGTTVSTKHGAVKTDHILFIASGAFHIAKPSDLLPELQGRLPIR
VELQALTRDDMRRILTEPEASLIKQYVALMKTEGVTLDITDDAIDALADVAVAVNSTVENIGARRLQTVMERVLDDISFT
ASDRTGETMRVDAAYVQQHIGDLAKNADLSRFIL

Sequences:

>Translated_434_residues
MTDFSPREIVSELDRFIVGQADAKRAVSIALRNRWRRLQLDTGLREEVLPKNILMIGPTGVGKTEIARRLAKLAGAPFLK
VEATKFTEVGYVGRDVEQIIRDLVEVAIHQVRERKRKDVAARAQLAAEERVLDALVGANSSAATRDSFRRKLRAGELNDK
EIEIETQSSGGGMPMFEIPGMPGAQMGAISIGDIFGKLGGRTKTRRVTVEASHDILIAEESDKLLDSDQLVQEAINVVEN
NGIVFLDEIDKICVRENRHGGDVSREGVQRDLLPLIEGTTVSTKHGAVKTDHILFIASGAFHIAKPSDLLPELQGRLPIR
VELQALTRDDMRRILTEPEASLIKQYVALMKTEGVTLDITDDAIDALADVAVAVNSTVENIGARRLQTVMERVLDDISFT
ASDRTGETMRVDAAYVQQHIGDLAKNADLSRFIL
>Mature_433_residues
TDFSPREIVSELDRFIVGQADAKRAVSIALRNRWRRLQLDTGLREEVLPKNILMIGPTGVGKTEIARRLAKLAGAPFLKV
EATKFTEVGYVGRDVEQIIRDLVEVAIHQVRERKRKDVAARAQLAAEERVLDALVGANSSAATRDSFRRKLRAGELNDKE
IEIETQSSGGGMPMFEIPGMPGAQMGAISIGDIFGKLGGRTKTRRVTVEASHDILIAEESDKLLDSDQLVQEAINVVENN
GIVFLDEIDKICVRENRHGGDVSREGVQRDLLPLIEGTTVSTKHGAVKTDHILFIASGAFHIAKPSDLLPELQGRLPIRV
ELQALTRDDMRRILTEPEASLIKQYVALMKTEGVTLDITDDAIDALADVAVAVNSTVENIGARRLQTVMERVLDDISFTA
SDRTGETMRVDAAYVQQHIGDLAKNADLSRFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790366, Length=446, Percent_Identity=58.2959641255605, Blast_Score=508, Evalue=1e-145,
Organism=Escherichia coli, GI1786642, Length=113, Percent_Identity=43.3628318584071, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6319704, Length=141, Percent_Identity=34.0425531914894, Blast_Score=73, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 47770; Mature: 47639

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFSPREIVSELDRFIVGQADAKRAVSIALRNRWRRLQLDTGLREEVLPKNILMIGPTG
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEECCCCCHHHHCCCCEEEECCCC
VGKTEIARRLAKLAGAPFLKVEATKFTEVGYVGRDVEQIIRDLVEVAIHQVRERKRKDVA
CCHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARAQLAAEERVLDALVGANSSAATRDSFRRKLRAGELNDKEIEIETQSSGGGMPMFEIPG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEECCC
MPGAQMGAISIGDIFGKLGGRTKTRRVTVEASHDILIAEESDKLLDSDQLVQEAINVVEN
CCCCCCCCEEHHHHHHHHCCCCEEEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHC
NGIVFLDEIDKICVRENRHGGDVSREGVQRDLLPLIEGTTVSTKHGAVKTDHILFIASGA
CCEEEEECHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCEECEEEEEECCC
FHIAKPSDLLPELQGRLPIRVELQALTRDDMRRILTEPEASLIKQYVALMKTEGVTLDIT
EEECCCHHHHHHHHCCCCEEEEHHHHCHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEC
DDAIDALADVAVAVNSTVENIGARRLQTVMERVLDDISFTASDRTGETMRVDAAYVQQHI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHH
GDLAKNADLSRFIL
HHHHHCCCHHHHCC
>Mature Secondary Structure 
TDFSPREIVSELDRFIVGQADAKRAVSIALRNRWRRLQLDTGLREEVLPKNILMIGPTG
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEECCCCCHHHHCCCCEEEECCCC
VGKTEIARRLAKLAGAPFLKVEATKFTEVGYVGRDVEQIIRDLVEVAIHQVRERKRKDVA
CCHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARAQLAAEERVLDALVGANSSAATRDSFRRKLRAGELNDKEIEIETQSSGGGMPMFEIPG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEECCC
MPGAQMGAISIGDIFGKLGGRTKTRRVTVEASHDILIAEESDKLLDSDQLVQEAINVVEN
CCCCCCCCEEHHHHHHHHCCCCEEEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHC
NGIVFLDEIDKICVRENRHGGDVSREGVQRDLLPLIEGTTVSTKHGAVKTDHILFIASGA
CCEEEEECHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCEECEEEEEECCC
FHIAKPSDLLPELQGRLPIRVELQALTRDDMRRILTEPEASLIKQYVALMKTEGVTLDIT
EEECCCHHHHHHHHCCCCEEEEHHHHCHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEC
DDAIDALADVAVAVNSTVENIGARRLQTVMERVLDDISFTASDRTGETMRVDAAYVQQHI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHH
GDLAKNADLSRFIL
HHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]