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The map label for this gene is ptsN [H]

Identifier: 146337284

GI number: 146337284

Start: 125439

End: 125900

Strand: Reverse

Name: ptsN [H]

Synonym: BRADO0111

Alternate gene names: 146337284

Gene position: 125900-125439 (Counterclockwise)

Preceding gene: 146337285

Following gene: 146337274

Centisome position: 1.69

GC content: 68.4

Gene sequence:

>462_bases
ATGCCGATTACCGATCTGGTCGCACCCGAGGCGATCCTGCCGGCTTTGAAGGTCATCAGTAAGAAGCAGGCGCTTCAGGA
GCTGGCGGCCCGCGCGGCCGCGCTGACCGGCCAGAACGAGCGCAGCGTGTTCGAGGTCCTGCTGCAGCGGGAGAAGCTCG
GCACCACGGCGGTCGGCTACGGCGTCGCGATTCCTCATGGCAAGCTGCCGAAGCTGGAGAAGCTGTTCGGCCTGTTCGCC
AGGCTGGAGCGGCCGATCGATTTCGAGGCGATGGACGGCCAGCCGGTCGACCTGGTGTTCCTGCTGCTCGCCCCCGAGGG
TGCCGGCGCCGATCATCTGAAGGCACTGGCGCGCATCGCCCGCCTGCTGCGCGACCAGGACGTCGCCAAGAAGCTGCGCG
CCTCGCGCGATGCCCAGGCGATCTATTCGGTGCTAGCCCTGCCACCGGCGTCCGCGGCCTGA

Upstream 100 bases:

>100_bases
ATCGGACACGGGGTAAGCCCGCCTGCCGCCTCAACTTCTTGACGCCGGTCTGTCCCGACCTATTTCGCACACTGACGGTT
CAATCACCTCGGAACGCCCT

Downstream 100 bases:

>100_bases
GGCCCTCTCCGGCTGTTCTAACTCCGCACTGATCGGCCGATGTAGCGCACCGTCTTGGCGCCGTCGTGGATGCTTGCGGT
TCCGAGCGTCGTGAAGCCGA

Product: nitrogen regulatory protein

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Nitrogen-metabolic PTS system EIIA component [H]

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MPITDLVAPEAILPALKVISKKQALQELAARAAALTGQNERSVFEVLLQREKLGTTAVGYGVAIPHGKLPKLEKLFGLFA
RLERPIDFEAMDGQPVDLVFLLLAPEGAGADHLKALARIARLLRDQDVAKKLRASRDAQAIYSVLALPPASAA

Sequences:

>Translated_153_residues
MPITDLVAPEAILPALKVISKKQALQELAARAAALTGQNERSVFEVLLQREKLGTTAVGYGVAIPHGKLPKLEKLFGLFA
RLERPIDFEAMDGQPVDLVFLLLAPEGAGADHLKALARIARLLRDQDVAKKLRASRDAQAIYSVLALPPASAA
>Mature_152_residues
PITDLVAPEAILPALKVISKKQALQELAARAAALTGQNERSVFEVLLQREKLGTTAVGYGVAIPHGKLPKLEKLFGLFAR
LERPIDFEAMDGQPVDLVFLLLAPEGAGADHLKALARIARLLRDQDVAKKLRASRDAQAIYSVLALPPASAA

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=131, Percent_Identity=43.5114503816794, Blast_Score=106, Evalue=5e-25,
Organism=Escherichia coli, GI1788726, Length=142, Percent_Identity=34.5070422535211, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1786951, Length=148, Percent_Identity=31.7567567567568, Blast_Score=70, Evalue=8e-14,
Organism=Escherichia coli, GI2367327, Length=103, Percent_Identity=31.0679611650485, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16410; Mature: 16279

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPITDLVAPEAILPALKVISKKQALQELAARAAALTGQNERSVFEVLLQREKLGTTAVGY
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHCC
GVAIPHGKLPKLEKLFGLFARLERPIDFEAMDGQPVDLVFLLLAPEGAGADHLKALARIA
CEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEECCCCCCHHHHHHHHHHH
RLLRDQDVAKKLRASRDAQAIYSVLALPPASAA
HHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PITDLVAPEAILPALKVISKKQALQELAARAAALTGQNERSVFEVLLQREKLGTTAVGY
CCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHCC
GVAIPHGKLPKLEKLFGLFARLERPIDFEAMDGQPVDLVFLLLAPEGAGADHLKALARIA
CEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEECCCCCCHHHHHHHHHHH
RLLRDQDVAKKLRASRDAQAIYSVLALPPASAA
HHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275; 1991712 [H]