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The map label for this gene is folC [H]

Identifier: 146337261

GI number: 146337261

Start: 104295

End: 105638

Strand: Reverse

Name: folC [H]

Synonym: BRADO0088

Alternate gene names: 146337261

Gene position: 105638-104295 (Counterclockwise)

Preceding gene: 146337262

Following gene: 146337260

Centisome position: 1.42

GC content: 66.22

Gene sequence:

>1344_bases
GTGAACGATCCCGCCAGCCAGCCCAAGCCTTCGCTCGACGAGCTGATCACGCGGCTCTCGGCGCTGCATCCGAGCAAGAT
CTCGCTCGGGCTGGAGCGCATGCACCGGCTGCTCGCCCAGCTCGATCATCCCGAGCGCAAGCTGCCGCCGGTGATCCATG
TCGCCGGCACCAATGGCAAGGGATCCACGGTCGCTTACCTCAGGGCGATGCTCGAAGCCGCGGGCCTGAGCGTCCACGTC
TTCACCTCGCCCTGGCTGGTCCGCATCAATGAGAGCTATCGCCTCGGACGAGTCGGTGGCGGCGTTCTGGTCGACGATGA
TGAATTGATCGACGCGCTCCTGCAATGCGAGCGAGTCAATGCCGGCGCGCCGATCACCTTCTTCGAGGTGAAGACCGTCG
CGGCCTTCGTGCTGTTCGCGAAACATCCCGCAGATGTCGTGCTGCTCGAAGTCGGCCTCGGCGGCCGGCTCGATTCGACC
AATGTGGTCGAACAGGTCGCGGCCTCCGTGCTGACGCCGATCAGCATGGACCACATGGAATTCCTCGGCGACACCCGCGC
GCTGATCGCGGCCGAGAAGGCTGCCATCATCAAGCGTGGCTGTCCGGTGATCTCGGCGGCGCAGGAGCCGGATGCGATGG
CGGTGATCGAGCGCGAGGCCAAGCGCCAGCGCGCGCCGCTGTTCGCGGCCGGCGAGAGCTGGCACGTCAATGTCGAGCGC
AACCGTCTCGTCTATCAGGACGATCGCGGGCTCCTGGATCTGCCGGCACCAAAGCTGTTCGGCCGGCATCAGTTCGACAA
TGCCGGTCTTGCGATCGCGACCTTGCGCGCGCTCGATCAATTCAAGCTGACGACCAAGGCGTTCGAGGACGGCATCGTCC
AAGCCGAATGGCCGGCGCGGATGCAGCGGCTGTCGAACGGCGCGCTCACCGCGATCGCGCCGCAGGGCGCGGAGATCTGG
CTCGACGGCGGCCACAATGCCGAAGGCGGCCGCGTCGTCGCGGCCGCGATCGGCGATCTCGAGGAGCGCGTCTCGCGGCA
GCTCGTCGTCATCGTTGGCATGATGGGCAACAAGGATGCAGCAGCCTTCCTTGCAAATTTCGCCGGGCTGACGCGCCACA
TCATCGTTGTCCCGATCCCGAACGAGAGCAAGGCGATGGCGCCGGCCGATCTCGCGTCGGCCGCGCGCACGCTCGGCATG
CGTATTGAGATCGCGGACGGTGTTGTTTCAGCGCTCGCCGGCGTCGCCCGGCTTGCCTATGAGGTGCCGCCGCGTATTCT
GATCACGGGGTCGCTTTATCTCGCAGGCCACGTGCTCGACCTCAACGGCACGCCGCCGACCTGA

Upstream 100 bases:

>100_bases
CCAAGGCGCCGGCCGTCGAACACGCCAAGCCCGCGCCGCAACTGCCGCCGCCGGCCAAGCCCGCCGAGACGGCGGAAGCG
CCGGCGGTCGCAACGAGCGC

Downstream 100 bases:

>100_bases
GGAAAAACAATTCATGCGCTTCGCGGCGATCGCCGATGTCCACGGCAATCATCTCGCGCTGGAGGCCGTGCTGGCGGACA
TCCGCGCGCAAGGGGTCACC

Product: folC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MNDPASQPKPSLDELITRLSALHPSKISLGLERMHRLLAQLDHPERKLPPVIHVAGTNGKGSTVAYLRAMLEAAGLSVHV
FTSPWLVRINESYRLGRVGGGVLVDDDELIDALLQCERVNAGAPITFFEVKTVAAFVLFAKHPADVVLLEVGLGGRLDST
NVVEQVAASVLTPISMDHMEFLGDTRALIAAEKAAIIKRGCPVISAAQEPDAMAVIEREAKRQRAPLFAAGESWHVNVER
NRLVYQDDRGLLDLPAPKLFGRHQFDNAGLAIATLRALDQFKLTTKAFEDGIVQAEWPARMQRLSNGALTAIAPQGAEIW
LDGGHNAEGGRVVAAAIGDLEERVSRQLVVIVGMMGNKDAAAFLANFAGLTRHIIVVPIPNESKAMAPADLASAARTLGM
RIEIADGVVSALAGVARLAYEVPPRILITGSLYLAGHVLDLNGTPPT

Sequences:

>Translated_447_residues
MNDPASQPKPSLDELITRLSALHPSKISLGLERMHRLLAQLDHPERKLPPVIHVAGTNGKGSTVAYLRAMLEAAGLSVHV
FTSPWLVRINESYRLGRVGGGVLVDDDELIDALLQCERVNAGAPITFFEVKTVAAFVLFAKHPADVVLLEVGLGGRLDST
NVVEQVAASVLTPISMDHMEFLGDTRALIAAEKAAIIKRGCPVISAAQEPDAMAVIEREAKRQRAPLFAAGESWHVNVER
NRLVYQDDRGLLDLPAPKLFGRHQFDNAGLAIATLRALDQFKLTTKAFEDGIVQAEWPARMQRLSNGALTAIAPQGAEIW
LDGGHNAEGGRVVAAAIGDLEERVSRQLVVIVGMMGNKDAAAFLANFAGLTRHIIVVPIPNESKAMAPADLASAARTLGM
RIEIADGVVSALAGVARLAYEVPPRILITGSLYLAGHVLDLNGTPPT
>Mature_447_residues
MNDPASQPKPSLDELITRLSALHPSKISLGLERMHRLLAQLDHPERKLPPVIHVAGTNGKGSTVAYLRAMLEAAGLSVHV
FTSPWLVRINESYRLGRVGGGVLVDDDELIDALLQCERVNAGAPITFFEVKTVAAFVLFAKHPADVVLLEVGLGGRLDST
NVVEQVAASVLTPISMDHMEFLGDTRALIAAEKAAIIKRGCPVISAAQEPDAMAVIEREAKRQRAPLFAAGESWHVNVER
NRLVYQDDRGLLDLPAPKLFGRHQFDNAGLAIATLRALDQFKLTTKAFEDGIVQAEWPARMQRLSNGALTAIAPQGAEIW
LDGGHNAEGGRVVAAAIGDLEERVSRQLVVIVGMMGNKDAAAFLANFAGLTRHIIVVPIPNESKAMAPADLASAARTLGM
RIEIADGVVSALAGVARLAYEVPPRILITGSLYLAGHVLDLNGTPPT

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=348, Percent_Identity=27.0114942528736, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI66932990, Length=348, Percent_Identity=27.0114942528736, Blast_Score=111, Evalue=2e-24,
Organism=Escherichia coli, GI1788654, Length=436, Percent_Identity=29.1284403669725, Blast_Score=145, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17553148, Length=439, Percent_Identity=25.0569476082005, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17553150, Length=439, Percent_Identity=25.0569476082005, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI71984923, Length=439, Percent_Identity=25.0569476082005, Blast_Score=115, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6323760, Length=435, Percent_Identity=28.2758620689655, Blast_Score=149, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6324815, Length=453, Percent_Identity=21.8543046357616, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24641571, Length=292, Percent_Identity=28.7671232876712, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24581568, Length=186, Percent_Identity=27.4193548387097, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47864; Mature: 47864

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDPASQPKPSLDELITRLSALHPSKISLGLERMHRLLAQLDHPERKLPPVIHVAGTNGK
CCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHCCCEEEEECCCCC
GSTVAYLRAMLEAAGLSVHVFTSPWLVRINESYRLGRVGGGVLVDDDELIDALLQCERVN
CHHHHHHHHHHHHCCCEEEEECCCEEEEECCCEEEEECCCCEEECCHHHHHHHHHHHHCC
AGAPITFFEVKTVAAFVLFAKHPADVVLLEVGLGGRLDSTNVVEQVAASVLTPISMDHME
CCCCEEEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHH
FLGDTRALIAAEKAAIIKRGCPVISAAQEPDAMAVIEREAKRQRAPLFAAGESWHVNVER
HHCCCHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHCCCEEECCCCEEEEEEC
NRLVYQDDRGLLDLPAPKLFGRHQFDNAGLAIATLRALDQFKLTTKAFEDGIVQAEWPAR
CEEEEECCCCEEECCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCHHH
MQRLSNGALTAIAPQGAEIWLDGGHNAEGGRVVAAAIGDLEERVSRQLVVIVGMMGNKDA
HHHHCCCCEEEECCCCCEEEEECCCCCCCCEEEEEHHHHHHHHHCCEEEEEEEECCCCHH
AAFLANFAGLTRHIIVVPIPNESKAMAPADLASAARTLGMRIEIADGVVSALAGVARLAY
HHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCEEEEEHHHHHHHHHHHHHHHH
EVPPRILITGSLYLAGHVLDLNGTPPT
HCCCCEEEEEEEEEEEEEEECCCCCCC
>Mature Secondary Structure
MNDPASQPKPSLDELITRLSALHPSKISLGLERMHRLLAQLDHPERKLPPVIHVAGTNGK
CCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHCCCEEEEECCCCC
GSTVAYLRAMLEAAGLSVHVFTSPWLVRINESYRLGRVGGGVLVDDDELIDALLQCERVN
CHHHHHHHHHHHHCCCEEEEECCCEEEEECCCEEEEECCCCEEECCHHHHHHHHHHHHCC
AGAPITFFEVKTVAAFVLFAKHPADVVLLEVGLGGRLDSTNVVEQVAASVLTPISMDHME
CCCCEEEEHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHH
FLGDTRALIAAEKAAIIKRGCPVISAAQEPDAMAVIEREAKRQRAPLFAAGESWHVNVER
HHCCCHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHCCCEEECCCCEEEEEEC
NRLVYQDDRGLLDLPAPKLFGRHQFDNAGLAIATLRALDQFKLTTKAFEDGIVQAEWPAR
CEEEEECCCCEEECCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEECCCHHH
MQRLSNGALTAIAPQGAEIWLDGGHNAEGGRVVAAAIGDLEERVSRQLVVIVGMMGNKDA
HHHHCCCCEEEECCCCCEEEEECCCCCCCCEEEEEHHHHHHHHHCCEEEEEEEECCCCHH
AAFLANFAGLTRHIIVVPIPNESKAMAPADLASAARTLGMRIEIADGVVSALAGVARLAY
HHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCEEEEEHHHHHHHHHHHHHHHH
EVPPRILITGSLYLAGHVLDLNGTPPT
HCCCCEEEEEEEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]