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The map label for this gene is gph [C]

Identifier: 146337195

GI number: 146337195

Start: 25530

End: 26207

Strand: Reverse

Name: gph [C]

Synonym: BRADO0021

Alternate gene names: 146337195

Gene position: 26207-25530 (Counterclockwise)

Preceding gene: 146337196

Following gene: 146337194

Centisome position: 0.35

GC content: 63.86

Gene sequence:

>678_bases
ATGTCCCACGTTCACGCAGTCCTGCTCGACCTCGACGGCACTCTGATCGACTCGCGCCCCGGCATCGCCGCGAGCGCGCT
GGCGGCGTTGCGGTCGCTCGGTCATGCGCCTGATCAAGCACTGGATATCACGGCCTTCATCGGTCCGCCCTTGCAGGACA
TGATGCGCGAGCTGCTGCAGGCTCATGGCGACCGTGTTGGCGGCGACCGTGTCGACGAAGCCGTCCTGGTCTATCGCCGG
CATTATGGTGATATCGGTCTGTTCGACTGCTCGCTCTATCCCGGCATTCGCGAGGCGCTGCAGGAGATGCATCGCGCCGG
GTTGCGGATCTATCTCGCGACGTCGAAGCGCGAGCTGTTCGCGCGCCGTATCCTGGAGCATCTCGATCTGGCGTCGTACT
TCCAAGCCATTCATGGCTCGGTGCCTTCAGGCGAGCTCGATCACAAGCCCGAGTTGCTCGCGCATATTCTCATGAAGCGG
AGTATCACCGCCGCGCATGGCGTGATGGTCGGCGACCGTCGTCATGACATCGCCGGCGCGCACGCCGTGGGCATGCGCAG
CCTCGGCGTGCTCTGGGGTTATGGCGGTCGGGAGGAGTTGGAAACCTCCGGTGCAGGCCGTCTGGTGGAAAGTCCCTCCG
ATCTGGCGGCAGCGGTGGTGACGATGTTGAAGAGATAG

Upstream 100 bases:

>100_bases
CAGGCCCTGATGCAGAGACTGAAGCGTCCTGCATGGGGTTGATACGACGGTCGGGCGCATCGGCGTTTGCGGCGCCCCTG
GCCGGGCGCTATGCTCGGTC

Downstream 100 bases:

>100_bases
CCGGTCGGCCCTGTGCGTGGCCAATCTTGCACCGGAACCGGAAAGCCGCGATGATCCGCGCAAGAACTCTCAGGGCAGGA
ATACCACGCAGATGGAATTC

Product: putative haloacid dehalogenase-like hydrolase family protein phosphoglycolate phosphatase

Products: NA

Alternate protein names: Nucleoside 5'-monophosphate phosphohydrolase [H]

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MSHVHAVLLDLDGTLIDSRPGIAASALAALRSLGHAPDQALDITAFIGPPLQDMMRELLQAHGDRVGGDRVDEAVLVYRR
HYGDIGLFDCSLYPGIREALQEMHRAGLRIYLATSKRELFARRILEHLDLASYFQAIHGSVPSGELDHKPELLAHILMKR
SITAAHGVMVGDRRHDIAGAHAVGMRSLGVLWGYGGREELETSGAGRLVESPSDLAAAVVTMLKR

Sequences:

>Translated_225_residues
MSHVHAVLLDLDGTLIDSRPGIAASALAALRSLGHAPDQALDITAFIGPPLQDMMRELLQAHGDRVGGDRVDEAVLVYRR
HYGDIGLFDCSLYPGIREALQEMHRAGLRIYLATSKRELFARRILEHLDLASYFQAIHGSVPSGELDHKPELLAHILMKR
SITAAHGVMVGDRRHDIAGAHAVGMRSLGVLWGYGGREELETSGAGRLVESPSDLAAAVVTMLKR
>Mature_224_residues
SHVHAVLLDLDGTLIDSRPGIAASALAALRSLGHAPDQALDITAFIGPPLQDMMRELLQAHGDRVGGDRVDEAVLVYRRH
YGDIGLFDCSLYPGIREALQEMHRAGLRIYLATSKRELFARRILEHLDLASYFQAIHGSVPSGELDHKPELLAHILMKRS
ITAAHGVMVGDRRHDIAGAHAVGMRSLGVLWGYGGREELETSGAGRLVESPSDLAAAVVTMLKR

Specific function: Specifically dephosphorylates nucleoside 5'- monophosphates to nucleosides and inorganic phosphate. Displays high activity toward 5'-UMP and 5'-IMP, significant activity against 5'-XMP and 5'-TMP, and low activity against 5'-CMP [H]

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=236, Percent_Identity=28.8135593220339, Blast_Score=87, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.5 [H]

Molecular weight: Translated: 24464; Mature: 24332

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHVHAVLLDLDGTLIDSRPGIAASALAALRSLGHAPDQALDITAFIGPPLQDMMRELLQ
CCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEHHCCCCHHHHHHHHHH
AHGDRVGGDRVDEAVLVYRRHYGDIGLFDCSLYPGIREALQEMHRAGLRIYLATSKRELF
HCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCEEEEEECCHHHHH
ARRILEHLDLASYFQAIHGSVPSGELDHKPELLAHILMKRSITAAHGVMVGDRRHDIAGA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCEEECCCCHHHHHH
HAVGMRSLGVLWGYGGREELETSGAGRLVESPSDLAAAVVTMLKR
HHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SHVHAVLLDLDGTLIDSRPGIAASALAALRSLGHAPDQALDITAFIGPPLQDMMRELLQ
CCCEEEEEECCCEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEHHCCCCHHHHHHHHHH
AHGDRVGGDRVDEAVLVYRRHYGDIGLFDCSLYPGIREALQEMHRAGLRIYLATSKRELF
HCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHCCEEEEEECCHHHHH
ARRILEHLDLASYFQAIHGSVPSGELDHKPELLAHILMKRSITAAHGVMVGDRRHDIAGA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCEEECCCCHHHHHH
HAVGMRSLGVLWGYGGREELETSGAGRLVESPSDLAAAVVTMLKR
HHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]