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The map label for this gene is argF [H]

Identifier: 146337196

GI number: 146337196

Start: 26298

End: 27095

Strand: Reverse

Name: argF [H]

Synonym: BRADO0022

Alternate gene names: 146337196

Gene position: 27095-26298 (Counterclockwise)

Preceding gene: 146337198

Following gene: 146337195

Centisome position: 0.36

GC content: 67.04

Gene sequence:

>798_bases
ATGCGTGCGCAGGAACTTGCAGTCTGCTGGCGGGCGCGCACCATGCCGCAAGCGCTCGCCGACCGGCGCATCGCGCTCGT
GGTCGATGATGGTGGCTGGCGCAACACCACCGCCTTCGATCTCGGCGTGCAAGCGATGGGCGGCCTCTGCGTGCACGCGC
CGATCCGCCTCAATGCCACGGAAGCGGTGGAGGATCTCGCCGCCTATCTCGACAACTGGTTCGATGCGGTGGTCTGCCGT
ACGCCCGAGCTGGAGCTGCTCTATGCCTTGTCCCACTGGGCGCAGGCGCCGGTGGTCAACGCGCGGACGCGGCAGAACCA
TCCCTGTGAGACGCTCGGTGACCTCGCCTTCCTGTGGCATCGGCGCGGCGCGATCGACGGATTGAAGATCGCCGTGGTGG
CGCCGGCCGCGAACATCCTGGGCTCGTGGATCGAGGCTGCGCAGATCCTTCCGATCGATGTCGTTCAGGTCTACCCAGCG
CGCTGGCATGCGCAGGGCGAGTCGCCGCGCTTCCACGTCACGACCGATCTCGACGCGTTGCAGGCCGCCGACGTGATCAT
CACGGATTGCTGGCCGATCGAAGAGGAGGCGAGCGTCCTGCAGGACTATCAGATCACGGCCGCGCTGTTGGATGGCCTGC
GGCCGGATCTGGAGTTCCTGCCTTGCCCGCCCGTGACACGTGGGCACGAGGTTTCTGACGACGCGATGCGGCATCGCGCC
TGTCGCGCGGTGCAGTCGAAGGCGTTCGTGTTGCACGCGCAGAATGCCGCGCTCGAATGGGTGTTCGGCAGGCCCTGA

Upstream 100 bases:

>100_bases
CGATCTGCGTGATCCCTGGACGTGGTGAGCGCGCATGACGGGCGATCGTGACATCCTGGCCCTCAGTGACCTCGATGGTT
CCGATATCCAAGGCATGCTG

Downstream 100 bases:

>100_bases
TGCAGAGACTGAAGCGTCCTGCATGGGGTTGATACGACGGTCGGGCGCATCGGCGTTTGCGGCGCCCCTGGCCGGGCGCT
ATGCTCGGTCATGTCCCACG

Product: putative aspartate/ornithine carbamoyltransferase family protein

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MRAQELAVCWRARTMPQALADRRIALVVDDGGWRNTTAFDLGVQAMGGLCVHAPIRLNATEAVEDLAAYLDNWFDAVVCR
TPELELLYALSHWAQAPVVNARTRQNHPCETLGDLAFLWHRRGAIDGLKIAVVAPAANILGSWIEAAQILPIDVVQVYPA
RWHAQGESPRFHVTTDLDALQAADVIITDCWPIEEEASVLQDYQITAALLDGLRPDLEFLPCPPVTRGHEVSDDAMRHRA
CRAVQSKAFVLHAQNAALEWVFGRP

Sequences:

>Translated_265_residues
MRAQELAVCWRARTMPQALADRRIALVVDDGGWRNTTAFDLGVQAMGGLCVHAPIRLNATEAVEDLAAYLDNWFDAVVCR
TPELELLYALSHWAQAPVVNARTRQNHPCETLGDLAFLWHRRGAIDGLKIAVVAPAANILGSWIEAAQILPIDVVQVYPA
RWHAQGESPRFHVTTDLDALQAADVIITDCWPIEEEASVLQDYQITAALLDGLRPDLEFLPCPPVTRGHEVSDDAMRHRA
CRAVQSKAFVLHAQNAALEWVFGRP
>Mature_265_residues
MRAQELAVCWRARTMPQALADRRIALVVDDGGWRNTTAFDLGVQAMGGLCVHAPIRLNATEAVEDLAAYLDNWFDAVVCR
TPELELLYALSHWAQAPVVNARTRQNHPCETLGDLAFLWHRRGAIDGLKIAVVAPAANILGSWIEAAQILPIDVVQVYPA
RWHAQGESPRFHVTTDLDALQAADVIITDCWPIEEEASVLQDYQITAALLDGLRPDLEFLPCPPVTRGHEVSDDAMRHRA
CRAVQSKAFVLHAQNAALEWVFGRP

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Escherichia coli, GI1790703, Length=234, Percent_Identity=25.2136752136752, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1786469, Length=238, Percent_Identity=26.890756302521, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 29352; Mature: 29352

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAQELAVCWRARTMPQALADRRIALVVDDGGWRNTTAFDLGVQAMGGLCVHAPIRLNAT
CCHHHHHHHHHHHCCHHHHHCCEEEEEEECCCCCCCEEHHHHHHHHCCEEEECCEEECHH
EAVEDLAAYLDNWFDAVVCRTPELELLYALSHWAQAPVVNARTRQNHPCETLGDLAFLWH
HHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
RRGAIDGLKIAVVAPAANILGSWIEAAQILPIDVVQVYPARWHAQGESPRFHVTTDLDAL
HCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCHHEEECCCCEECCCCCCCEEEECCCHHH
QAADVIITDCWPIEEEASVLQDYQITAALLDGLRPDLEFLPCPPVTRGHEVSDDAMRHRA
HHCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCHHHHHHHH
CRAVQSKAFVLHAQNAALEWVFGRP
HHHHHHCEEEEEECCCEEEEEECCC
>Mature Secondary Structure
MRAQELAVCWRARTMPQALADRRIALVVDDGGWRNTTAFDLGVQAMGGLCVHAPIRLNAT
CCHHHHHHHHHHHCCHHHHHCCEEEEEEECCCCCCCEEHHHHHHHHCCEEEECCEEECHH
EAVEDLAAYLDNWFDAVVCRTPELELLYALSHWAQAPVVNARTRQNHPCETLGDLAFLWH
HHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
RRGAIDGLKIAVVAPAANILGSWIEAAQILPIDVVQVYPARWHAQGESPRFHVTTDLDAL
HCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCHHEEECCCCEECCCCCCCEEEECCCHHH
QAADVIITDCWPIEEEASVLQDYQITAALLDGLRPDLEFLPCPPVTRGHEVSDDAMRHRA
HHCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCHHHHHHHH
CRAVQSKAFVLHAQNAALEWVFGRP
HHHHHHCEEEEEECCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA