Click here to switch to the map view.

The map label for this gene is pbuE [H]

Identifier: 146301703

GI number: 146301703

Start: 4735355

End: 4736596

Strand: Direct

Name: pbuE [H]

Synonym: Fjoh_3965

Alternate gene names: 146301703

Gene position: 4735355-4736596 (Clockwise)

Preceding gene: 146301702

Following gene: 146301708

Centisome position: 77.67

GC content: 36.31

Gene sequence:

>1242_bases
ATGAACGAAAATCAAACTCAGAAACCCAGTTTTAACGGCTATCAAAAACTGGTTATATTTTTATTAGCCATTACACAATT
TACTGTTGTATTAGATTTCATGGTAATGTCGCCTCTTGGAGATATTATGATGAAGACTTTAAAAATCACGGCCTCACAAT
TTGGTGTTGCCGTTTCTGCTTATGCTTTCAGCGCCGGAGTCTCTGGTTTGCTTACTGCAGGTTTTGCAGATAAATTTGAC
CGAAAAAAACTGCTGCTTTTCTTTTACATCGGTTTTACTATAGGAACTTTATTTTGTGCTTTGGCACCTAACTTTTTCCT
TTTAGTTGCCGCAAGAATCTTTACCGGATTATTTGGCGGTGTAATTGGTTCTATTTCTCTTGCTATTGTTGCTGATTTAT
TCAGCTTACAGCAGAGAGGAAAAGTTATGGGATTTATTCAAATGGGATTTGGTGTAAGCCAGGTTCTTGGAATCCCGATT
GGTTTATACATTGCAAATGCATGGGGATGGCACGTACCTTTTTTATGGATTGCAGCAATGGCGGCTATAATCACAATCAT
TCTTTATGCTAAATTACAGCCTATTACAGAGCACTTGAAATTAAAACAAGAAAAAACAGCAATTATGCATTTAGTGCATA
CCATTTCAAAAAAGAATTATCAAATTGGTTTTCTGTCTACTGCCCTGCTTTCTATTGGCGGCTTTATGATGATGCCTTTT
GGAAGCGCTTTTGCCATTAACAACCTTAAAGTTACGAATGAAGAATTGCCTTTGTTATTCATGATTACAGGAATCGCTAC
TCTATTTACCATGCCTTTTATTGGTAAACTGAGTGACAAGGTAAATAAATTTAAAATCTTTGCTTTTGCAACCGTATCTG
CTGTGATTTTAATTAATATTTACTCTCATTTTGGTCCTACGCCTTTCTGGATTGTTTTGGTTATAAATGTATTTATGATG
GCAACTATCATGAGCAGAATGGTTCCGTCGACAACCTTAACTTCTGCTGTTCCGGATCCGCAGGATCGTGGTGCGTTTAT
GAGTATTAACTCCTCTTTACAGCAAATGGCCGGAGGTTTTGGTGCTGTAATTGCCGGATTAATTATCTATCAAAAAGACA
GTTATGCACCGCTGGAACATTATGATATTTTAGCAATGGTAGCTTCTGCCATTATGTTACTTACGATTTACTTAGTATTT
AAAGTGAGTAAGATTGTGGAAAGCAAACCAAATAAATTCTAA

Upstream 100 bases:

>100_bases
TGACTGTGTACTTAGAATTATAAGCGAAAAACTAAACGCAGGCCAAATAATCGATTTATTGAATTTTCACGATTTTGACT
GCAAAGAATTACAATAATAT

Downstream 100 bases:

>100_bases
TTTCTATAGAATAAAATCTATAAAAAGAAAAACTCCCAATACAATTGGGAGTTTTTTTGTTTTACAACCTAATTCTGAAT
CTGTGTGATTCTCATATAAT

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MNENQTQKPSFNGYQKLVIFLLAITQFTVVLDFMVMSPLGDIMMKTLKITASQFGVAVSAYAFSAGVSGLLTAGFADKFD
RKKLLLFFYIGFTIGTLFCALAPNFFLLVAARIFTGLFGGVIGSISLAIVADLFSLQQRGKVMGFIQMGFGVSQVLGIPI
GLYIANAWGWHVPFLWIAAMAAIITIILYAKLQPITEHLKLKQEKTAIMHLVHTISKKNYQIGFLSTALLSIGGFMMMPF
GSAFAINNLKVTNEELPLLFMITGIATLFTMPFIGKLSDKVNKFKIFAFATVSAVILINIYSHFGPTPFWIVLVINVFMM
ATIMSRMVPSTTLTSAVPDPQDRGAFMSINSSLQQMAGGFGAVIAGLIIYQKDSYAPLEHYDILAMVASAIMLLTIYLVF
KVSKIVESKPNKF

Sequences:

>Translated_413_residues
MNENQTQKPSFNGYQKLVIFLLAITQFTVVLDFMVMSPLGDIMMKTLKITASQFGVAVSAYAFSAGVSGLLTAGFADKFD
RKKLLLFFYIGFTIGTLFCALAPNFFLLVAARIFTGLFGGVIGSISLAIVADLFSLQQRGKVMGFIQMGFGVSQVLGIPI
GLYIANAWGWHVPFLWIAAMAAIITIILYAKLQPITEHLKLKQEKTAIMHLVHTISKKNYQIGFLSTALLSIGGFMMMPF
GSAFAINNLKVTNEELPLLFMITGIATLFTMPFIGKLSDKVNKFKIFAFATVSAVILINIYSHFGPTPFWIVLVINVFMM
ATIMSRMVPSTTLTSAVPDPQDRGAFMSINSSLQQMAGGFGAVIAGLIIYQKDSYAPLEHYDILAMVASAIMLLTIYLVF
KVSKIVESKPNKF
>Mature_413_residues
MNENQTQKPSFNGYQKLVIFLLAITQFTVVLDFMVMSPLGDIMMKTLKITASQFGVAVSAYAFSAGVSGLLTAGFADKFD
RKKLLLFFYIGFTIGTLFCALAPNFFLLVAARIFTGLFGGVIGSISLAIVADLFSLQQRGKVMGFIQMGFGVSQVLGIPI
GLYIANAWGWHVPFLWIAAMAAIITIILYAKLQPITEHLKLKQEKTAIMHLVHTISKKNYQIGFLSTALLSIGGFMMMPF
GSAFAINNLKVTNEELPLLFMITGIATLFTMPFIGKLSDKVNKFKIFAFATVSAVILINIYSHFGPTPFWIVLVINVFMM
ATIMSRMVPSTTLTSAVPDPQDRGAFMSINSSLQQMAGGFGAVIAGLIIYQKDSYAPLEHYDILAMVASAIMLLTIYLVF
KVSKIVESKPNKF

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine, 6-mercaptopurine and 2-fluoroadenine. Therefore plays a role

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45295; Mature: 45295

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure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HHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]