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The map label for this gene is phoA [H]

Identifier: 146300993

GI number: 146300993

Start: 3883517

End: 3885340

Strand: Reverse

Name: phoA [H]

Synonym: Fjoh_3249

Alternate gene names: 146300993

Gene position: 3885340-3883517 (Counterclockwise)

Preceding gene: 146300994

Following gene: 146300991

Centisome position: 63.73

GC content: 36.07

Gene sequence:

>1824_bases
ATGAAAAAAATAATCACCCTTTTTTGCCTCCAGCTTTTTGTATTCGTGCAGGCGCAGGAATACACATCATCAAACATTCA
TTCTCATAACGATTATGCCAGTGCGCTTCCGTTTTACGGAGCTTATTCTAACGAAGCAGGCGTTATTGAGGCCGATGTAT
TTTTAGTAAACAATGAATTGTTTGTTGCCCATACATCAAAAGAAATTGCACAGCATAATACATTAAAAAGTATGTATTTG
GAACCGCTTTCTGCTAAATTAAAGAATCTGGGCTCAAAAGCATATCCAGGCAATAAGCCTTTAATTTTAATGATCGACAT
TAAATCTGATGCCGATTCGACTTTAAAAGTAATTGCACAGCAGTTAAAAACTTATCCTGATATTACAGTAAATAAAAACA
TAAAAGTGGTTATTTCAGGAAACAGACCAAACCCTGCACAATGGAAAGACTATCCGGAGTTTATTTATTTTGACGGAAGA
CTGAATGAAAACTATACTGCAGATCAATTAGCAAGAGTTGAAATGATCAGTGAAGATTTGCATGAATTAACCGTTTGGAA
CGGAAAAGGAGTTCTAACACAAGCCGATTTAGAAAAAATCCAGTCAACGATTAGAAAAGTTCACAATCAGAATAAAAAAA
TACGTTTTTGGGCTACTCAGGATAATGTAAATACCTGGATGACTTTAATGAACCTGAAAGTTGATTTTATAGGAACAGAT
AATGTGGCAGAATTAACGCATTTTATCAACAATATCAAATCAACATTTTATCAAAATACAGAGTTTCATGAGGCGTATGT
TCCTAAAAATGCATCGGCTTTCGCCAAAAAGAAACCAAAAAATGTTATTCTTTTAATTGGAGACGGAATGGGGTTAACGC
AGATTTACTCTGGTTACACAGCGAATAAAGGACAGTTAAGCCTTTTTAATATTCCTACGCAGGGATTTTCGATAACCAAA
GCATCAGACAGTTATATTACAGATTCTGCTGCAGGCGCAACGGCAATGGCAACAGGACATAAAACCAACAACAGATTTAT
AAGCGTTGACGAAAGCGGCAAAACATTAGAATTAATTACACAGCAATTAGCTAAGAAAAATTACAAAACCGCTATTATTT
CGGCAGGAAATATTACAGATGCTACTCCGGCCGCTTTTTATGCACATCAGCCGGAAAGAAGTTACAGTGAACCAATTGCT
TTTGATTTCCTGAGTAATCCATCGGATATATTAATTGGAGGCGGAACAAAAGAGTTTAAGGCCAGAAAAGACGGAAAAGA
TTTGTCTAAAACCCTGACCGAAAAAGGATATACTTTCTCTGATAAATTCAGTTCTTTGGATACAATCAAAAACAGCAGAT
TTGTGGTTTTAGAAGATGCAGCAGTAGTTTCTATGAAAAACGGAAGAGGTGATTTTTTAACCAAATCATTTGCCAAGACA
GTCAATACTTTTTCTAAAACAAAGAATCCGTTTTTCATTATGGCCGAAGGTGCTCAAATCGATTACGGCGGACACAGCAA
TAATGTAGAATATGTGGTTCGCGAAATGCTTGATTTTGACAAACTAGTAGGGCAGGCGATGGAATTTGTAGACCATAATC
CAGAAACACTTTTAATTGTAACAGCAGATCACGAAACAGGCGGATTATCTTTAATTGACGGAAGTATCGAAAAAGGTTAT
GTTCACGGAAGTTTTAGTACAAACGATCATACAGCAGTTCCGGTTCCGGTTTTTGCTTACGGAGCAGGTGCTCAAAACTT
TTCAGGAGTGTATCAAAACACAGCGATTTATACGAAGATTTTAGAATCGCTTTCAATAAAATAA

Upstream 100 bases:

>100_bases
TTAATATCATAAAAACCAGTCTTTTTAAAGGACTGGTTTATGTTTTCGAATTAATTTCTAAAAGTTACCTACGGCTTTAG
AAAAATAAAACCCATTTTCG

Downstream 100 bases:

>100_bases
TTCAATATTTTTCAAATAAAAAGCCCTTGTTAATGCAGGGGCTTTTTGTTTATATTTTCGATAATAAGATCAATTCGCTG
TTGAAGTGTTAAATCGCCTG

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase IV; APase IV [H]

Number of amino acids: Translated: 607; Mature: 607

Protein sequence:

>607_residues
MKKIITLFCLQLFVFVQAQEYTSSNIHSHNDYASALPFYGAYSNEAGVIEADVFLVNNELFVAHTSKEIAQHNTLKSMYL
EPLSAKLKNLGSKAYPGNKPLILMIDIKSDADSTLKVIAQQLKTYPDITVNKNIKVVISGNRPNPAQWKDYPEFIYFDGR
LNENYTADQLARVEMISEDLHELTVWNGKGVLTQADLEKIQSTIRKVHNQNKKIRFWATQDNVNTWMTLMNLKVDFIGTD
NVAELTHFINNIKSTFYQNTEFHEAYVPKNASAFAKKKPKNVILLIGDGMGLTQIYSGYTANKGQLSLFNIPTQGFSITK
ASDSYITDSAAGATAMATGHKTNNRFISVDESGKTLELITQQLAKKNYKTAIISAGNITDATPAAFYAHQPERSYSEPIA
FDFLSNPSDILIGGGTKEFKARKDGKDLSKTLTEKGYTFSDKFSSLDTIKNSRFVVLEDAAVVSMKNGRGDFLTKSFAKT
VNTFSKTKNPFFIMAEGAQIDYGGHSNNVEYVVREMLDFDKLVGQAMEFVDHNPETLLIVTADHETGGLSLIDGSIEKGY
VHGSFSTNDHTAVPVPVFAYGAGAQNFSGVYQNTAIYTKILESLSIK

Sequences:

>Translated_607_residues
MKKIITLFCLQLFVFVQAQEYTSSNIHSHNDYASALPFYGAYSNEAGVIEADVFLVNNELFVAHTSKEIAQHNTLKSMYL
EPLSAKLKNLGSKAYPGNKPLILMIDIKSDADSTLKVIAQQLKTYPDITVNKNIKVVISGNRPNPAQWKDYPEFIYFDGR
LNENYTADQLARVEMISEDLHELTVWNGKGVLTQADLEKIQSTIRKVHNQNKKIRFWATQDNVNTWMTLMNLKVDFIGTD
NVAELTHFINNIKSTFYQNTEFHEAYVPKNASAFAKKKPKNVILLIGDGMGLTQIYSGYTANKGQLSLFNIPTQGFSITK
ASDSYITDSAAGATAMATGHKTNNRFISVDESGKTLELITQQLAKKNYKTAIISAGNITDATPAAFYAHQPERSYSEPIA
FDFLSNPSDILIGGGTKEFKARKDGKDLSKTLTEKGYTFSDKFSSLDTIKNSRFVVLEDAAVVSMKNGRGDFLTKSFAKT
VNTFSKTKNPFFIMAEGAQIDYGGHSNNVEYVVREMLDFDKLVGQAMEFVDHNPETLLIVTADHETGGLSLIDGSIEKGY
VHGSFSTNDHTAVPVPVFAYGAGAQNFSGVYQNTAIYTKILESLSIK
>Mature_607_residues
MKKIITLFCLQLFVFVQAQEYTSSNIHSHNDYASALPFYGAYSNEAGVIEADVFLVNNELFVAHTSKEIAQHNTLKSMYL
EPLSAKLKNLGSKAYPGNKPLILMIDIKSDADSTLKVIAQQLKTYPDITVNKNIKVVISGNRPNPAQWKDYPEFIYFDGR
LNENYTADQLARVEMISEDLHELTVWNGKGVLTQADLEKIQSTIRKVHNQNKKIRFWATQDNVNTWMTLMNLKVDFIGTD
NVAELTHFINNIKSTFYQNTEFHEAYVPKNASAFAKKKPKNVILLIGDGMGLTQIYSGYTANKGQLSLFNIPTQGFSITK
ASDSYITDSAAGATAMATGHKTNNRFISVDESGKTLELITQQLAKKNYKTAIISAGNITDATPAAFYAHQPERSYSEPIA
FDFLSNPSDILIGGGTKEFKARKDGKDLSKTLTEKGYTFSDKFSSLDTIKNSRFVVLEDAAVVSMKNGRGDFLTKSFAKT
VNTFSKTKNPFFIMAEGAQIDYGGHSNNVEYVVREMLDFDKLVGQAMEFVDHNPETLLIVTADHETGGLSLIDGSIEKGY
VHGSFSTNDHTAVPVPVFAYGAGAQNFSGVYQNTAIYTKILESLSIK

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI48994877, Length=414, Percent_Identity=26.8115942028986, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320689, Length=318, Percent_Identity=32.0754716981132, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI18859923, Length=295, Percent_Identity=28.135593220339, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21355981, Length=374, Percent_Identity=26.7379679144385, Blast_Score=93, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24657827, Length=337, Percent_Identity=25.8160237388724, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24651554, Length=339, Percent_Identity=25.9587020648968, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21355151, Length=324, Percent_Identity=24.0740740740741, Blast_Score=74, Evalue=4e-13,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 67339; Mature: 67339

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIITLFCLQLFVFVQAQEYTSSNIHSHNDYASALPFYGAYSNEAGVIEADVFLVNNEL
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEEEEEECEE
FVAHTSKEIAQHNTLKSMYLEPLSAKLKNLGSKAYPGNKPLILMIDIKSDADSTLKVIAQ
EEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHH
QLKTYPDITVNKNIKVVISGNRPNPAQWKDYPEFIYFDGRLNENYTADQLARVEMISEDL
HHHCCCCEEECCCEEEEEECCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHH
HELTVWNGKGVLTQADLEKIQSTIRKVHNQNKKIRFWATQDNVNTWMTLMNLKVDFIGTD
HHEEEECCCCCEEHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHEEEEEEEEEEEECCC
NVAELTHFINNIKSTFYQNTEFHEAYVPKNASAFAKKKPKNVILLIGDGMGLTQIYSGYT
CHHHHHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHCCCCEEEEEEECCCCHHHHHCCCC
ANKGQLSLFNIPTQGFSITKASDSYITDSAAGATAMATGHKTNNRFISVDESGKTLELIT
CCCCCEEEEECCCCCEEEEECCCCEECCCCCCCEEEECCCCCCCEEEEECCCCCHHHHHH
QQLAKKNYKTAIISAGNITDATPAAFYAHQPERSYSEPIAFDFLSNPSDILIGGGTKEFK
HHHHHCCCCEEEEECCCCCCCCCCHHEECCCCCCCCCCEEEEECCCCCCEEEECCCHHHH
ARKDGKDLSKTLTEKGYTFSDKFSSLDTIKNSRFVVLEDAAVVSMKNGRGDFLTKSFAKT
CCCCCHHHHHHHHHCCCEEHHHHHHHHHHCCCCEEEEECCEEEEECCCCCCHHHHHHHHH
VNTFSKTKNPFFIMAEGAQIDYGGHSNNVEYVVREMLDFDKLVGQAMEFVDHNPETLLIV
HHHHHCCCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
TADHETGGLSLIDGSIEKGYVHGSFSTNDHTAVPVPVFAYGAGAQNFSGVYQNTAIYTKI
EECCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHH
LESLSIK
HHHHCCC
>Mature Secondary Structure
MKKIITLFCLQLFVFVQAQEYTSSNIHSHNDYASALPFYGAYSNEAGVIEADVFLVNNEL
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEEEEEECEE
FVAHTSKEIAQHNTLKSMYLEPLSAKLKNLGSKAYPGNKPLILMIDIKSDADSTLKVIAQ
EEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHH
QLKTYPDITVNKNIKVVISGNRPNPAQWKDYPEFIYFDGRLNENYTADQLARVEMISEDL
HHHCCCCEEECCCEEEEEECCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHH
HELTVWNGKGVLTQADLEKIQSTIRKVHNQNKKIRFWATQDNVNTWMTLMNLKVDFIGTD
HHEEEECCCCCEEHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHEEEEEEEEEEEECCC
NVAELTHFINNIKSTFYQNTEFHEAYVPKNASAFAKKKPKNVILLIGDGMGLTQIYSGYT
CHHHHHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHCCCCEEEEEEECCCCHHHHHCCCC
ANKGQLSLFNIPTQGFSITKASDSYITDSAAGATAMATGHKTNNRFISVDESGKTLELIT
CCCCCEEEEECCCCCEEEEECCCCEECCCCCCCEEEECCCCCCCEEEEECCCCCHHHHHH
QQLAKKNYKTAIISAGNITDATPAAFYAHQPERSYSEPIAFDFLSNPSDILIGGGTKEFK
HHHHHCCCCEEEEECCCCCCCCCCHHEECCCCCCCCCCEEEEECCCCCCEEEECCCHHHH
ARKDGKDLSKTLTEKGYTFSDKFSSLDTIKNSRFVVLEDAAVVSMKNGRGDFLTKSFAKT
CCCCCHHHHHHHHHCCCEEHHHHHHHHHHCCCCEEEEECCEEEEECCCCCCHHHHHHHHH
VNTFSKTKNPFFIMAEGAQIDYGGHSNNVEYVVREMLDFDKLVGQAMEFVDHNPETLLIV
HHHHHCCCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
TADHETGGLSLIDGSIEKGYVHGSFSTNDHTAVPVPVFAYGAGAQNFSGVYQNTAIYTKI
EECCCCCCEEEEECCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHH
LESLSIK
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113174; 9579061; 9384377; 1898729; 2125017; 2105301; 8144469 [H]