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The map label for this gene is lcfB [H]

Identifier: 146300922

GI number: 146300922

Start: 3782766

End: 3784181

Strand: Direct

Name: lcfB [H]

Synonym: Fjoh_3177

Alternate gene names: 146300922

Gene position: 3782766-3784181 (Clockwise)

Preceding gene: 146300921

Following gene: 146300923

Centisome position: 62.04

GC content: 35.1

Gene sequence:

>1416_bases
ATGGGACTTTATGATTTAATTAAAAAAAATAAAAAATTAAATTTTATAGATGCAAACACTAAGGTTGTCAAAACATTTAA
CGATATACACGAATCTTTAGATATTGAAGATCTTCGTGCAGTTGTTTTCATTTACAATGATAATCAACTGCCGGCAATCG
AAGCATTTTTGAATTTTTATCAAAAAAAATATACAGTAGCCCTGCTTAATGCGGGGCTGCATCCTGAATTTAAACAAAAC
CTGGAAAACGAATACAAACCAGCATATATCTTCGATCCGTCTAGAACGATTGTTGAAGATTATGATTTAAAACAGGTATC
AAAAGACATATCTGTTTTTAAAAGAAAAGAAATTCGTCTCTACCCTATTCATGGCGATGTTAAGCTTTTAATGAGTACAT
CAGGAACCACAGGGTCGCCAAAATTTGTGAAAATTTCAGATGATAATCTGGTACAGAATGCTTTATCTATTATGGAGTAC
ATGCCAATAACGGAACACGATGTTGTTCCGTTAAATGTACCTATTAATTTTGTGTATGGCTTTTCGATTTTTACAACAAA
CTGTATAAAAGCAAATACAATTGTATGTACAGAAAGAGATGTGCTTCAAAAAGAATTCTGGTCAGATCTTGCAGAATATG
GCTACTCGACCTTAAGCGGCGTTCCTTATTTTTATGAGCTGCTGCATCGTTTTGGCTTCTTTAAAAAAGATACACCTTCT
GTACGTTATTTCACCCATACGGGAGGAATGCTGAACCAAGAACTGGCAAACGTGATTTCAAATTATACCAATATTTACGA
TAAACAGTTTTTTGCGCAATACGGACAAACTGAAGCCAGCGGCAGAATGGCTTATTTACCGCCAAAAGATTTTGAGACGA
AAGGAACATCAATAGGTTTTCCCGTCAAAAATGGAAGATTTGAAATTGATGAAAACACAGGAGAACTTATTTATTACGGT
CCTAATGTTTCCGGAGGTTATGCCAATAACATTTTCGATCTTCAGCATTATGACCAGACAGATAAACTTTATACCGGCGA
TCTGGCAGAAAAAGATGAATTGGGCTACTATCATATTAAAGGACGCATTAAACGTATTATGAAACTATTTGGAGTTCGTT
TAAATCTTGATGAGGTTGAGGTTTTGCTTAAAAATCAACTGGGAGGCCAGACTTTTATCTGTATAAGTGTTCAGGATAAA
TATCTTGGTGTTATACATCAGGATGCTTCTTTACTTCGCGAAACCATAACACAGGTAATTAAGGAAAAACTGGGATTGCA
TGCCAGCTCAATAAAAAACTATTATTTCGATCAAGTGCCGCTTACTGTTAACGGCAAAGTCGATTATCCGGCTATAAACA
AATACATCAACGAAAACGAAAATGTAGCTACAGCTGCAAAAACAGTTGTCGGGTAA

Upstream 100 bases:

>100_bases
TTTCTATGGAAGATATTCTCGAAATGGGAAACATCGATAAAATAAAAGCAATATTGAAGAAAAACGGAGTAGAAATCCAA
TAATTCAAAAAGAAAAAAGT

Downstream 100 bases:

>100_bases
TTTAAATATGTAACTAACAATTAGGCTAAAACCTAAAATAAAAAAACAAAACCATGGAACAGCAAAGCAAAAGAATTCCG
GCACTGGATTTAATAAAAGG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MGLYDLIKKNKKLNFIDANTKVVKTFNDIHESLDIEDLRAVVFIYNDNQLPAIEAFLNFYQKKYTVALLNAGLHPEFKQN
LENEYKPAYIFDPSRTIVEDYDLKQVSKDISVFKRKEIRLYPIHGDVKLLMSTSGTTGSPKFVKISDDNLVQNALSIMEY
MPITEHDVVPLNVPINFVYGFSIFTTNCIKANTIVCTERDVLQKEFWSDLAEYGYSTLSGVPYFYELLHRFGFFKKDTPS
VRYFTHTGGMLNQELANVISNYTNIYDKQFFAQYGQTEASGRMAYLPPKDFETKGTSIGFPVKNGRFEIDENTGELIYYG
PNVSGGYANNIFDLQHYDQTDKLYTGDLAEKDELGYYHIKGRIKRIMKLFGVRLNLDEVEVLLKNQLGGQTFICISVQDK
YLGVIHQDASLLRETITQVIKEKLGLHASSIKNYYFDQVPLTVNGKVDYPAINKYINENENVATAAKTVVG

Sequences:

>Translated_471_residues
MGLYDLIKKNKKLNFIDANTKVVKTFNDIHESLDIEDLRAVVFIYNDNQLPAIEAFLNFYQKKYTVALLNAGLHPEFKQN
LENEYKPAYIFDPSRTIVEDYDLKQVSKDISVFKRKEIRLYPIHGDVKLLMSTSGTTGSPKFVKISDDNLVQNALSIMEY
MPITEHDVVPLNVPINFVYGFSIFTTNCIKANTIVCTERDVLQKEFWSDLAEYGYSTLSGVPYFYELLHRFGFFKKDTPS
VRYFTHTGGMLNQELANVISNYTNIYDKQFFAQYGQTEASGRMAYLPPKDFETKGTSIGFPVKNGRFEIDENTGELIYYG
PNVSGGYANNIFDLQHYDQTDKLYTGDLAEKDELGYYHIKGRIKRIMKLFGVRLNLDEVEVLLKNQLGGQTFICISVQDK
YLGVIHQDASLLRETITQVIKEKLGLHASSIKNYYFDQVPLTVNGKVDYPAINKYINENENVATAAKTVVG
>Mature_470_residues
GLYDLIKKNKKLNFIDANTKVVKTFNDIHESLDIEDLRAVVFIYNDNQLPAIEAFLNFYQKKYTVALLNAGLHPEFKQNL
ENEYKPAYIFDPSRTIVEDYDLKQVSKDISVFKRKEIRLYPIHGDVKLLMSTSGTTGSPKFVKISDDNLVQNALSIMEYM
PITEHDVVPLNVPINFVYGFSIFTTNCIKANTIVCTERDVLQKEFWSDLAEYGYSTLSGVPYFYELLHRFGFFKKDTPSV
RYFTHTGGMLNQELANVISNYTNIYDKQFFAQYGQTEASGRMAYLPPKDFETKGTSIGFPVKNGRFEIDENTGELIYYGP
NVSGGYANNIFDLQHYDQTDKLYTGDLAEKDELGYYHIKGRIKRIMKLFGVRLNLDEVEVLLKNQLGGQTFICISVQDKY
LGVIHQDASLLRETITQVIKEKLGLHASSIKNYYFDQVPLTVNGKVDYPAINKYINENENVATAAKTVVG

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788107, Length=352, Percent_Identity=24.4318181818182, Blast_Score=82, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6319699, Length=346, Percent_Identity=24.8554913294798, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI18859661, Length=349, Percent_Identity=23.2091690544413, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 53848; Mature: 53717

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLYDLIKKNKKLNFIDANTKVVKTFNDIHESLDIEDLRAVVFIYNDNQLPAIEAFLNFY
CCHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHH
QKKYTVALLNAGLHPEFKQNLENEYKPAYIFDPSRTIVEDYDLKQVSKDISVFKRKEIRL
HHHEEEEEEECCCCHHHHHHHHHCCCCEEEECCCCHHHHCCCHHHHHHHHHHHCCCCEEE
YPIHGDVKLLMSTSGTTGSPKFVKISDDNLVQNALSIMEYMPITEHDVVPLNVPINFVYG
EEECCCEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCEEEEECCEEEEEE
FSIFTTNCIKANTIVCTERDVLQKEFWSDLAEYGYSTLSGVPYFYELLHRFGFFKKDTPS
EHHHEECCEECCEEEECCHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHCCCCCCCCC
VRYFTHTGGMLNQELANVISNYTNIYDKQFFAQYGQTEASGRMAYLPPKDFETKGTSIGF
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCEECC
PVKNGRFEIDENTGELIYYGPNVSGGYANNIFDLQHYDQTDKLYTGDLAEKDELGYYHIK
CCCCCEEEEECCCCCEEEECCCCCCCCCCCEEECCCCCCCCCEEECCCCCCCCCCEEEHH
GRIKRIMKLFGVRLNLDEVEVLLKNQLGGQTFICISVQDKYLGVIHQDASLLRETITQVI
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCEEEEHHHHHHHHHHHHHHH
KEKLGLHASSIKNYYFDQVPLTVNGKVDYPAINKYINENENVATAAKTVVG
HHHHCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure 
GLYDLIKKNKKLNFIDANTKVVKTFNDIHESLDIEDLRAVVFIYNDNQLPAIEAFLNFY
CHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHH
QKKYTVALLNAGLHPEFKQNLENEYKPAYIFDPSRTIVEDYDLKQVSKDISVFKRKEIRL
HHHEEEEEEECCCCHHHHHHHHHCCCCEEEECCCCHHHHCCCHHHHHHHHHHHCCCCEEE
YPIHGDVKLLMSTSGTTGSPKFVKISDDNLVQNALSIMEYMPITEHDVVPLNVPINFVYG
EEECCCEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCEEEEECCEEEEEE
FSIFTTNCIKANTIVCTERDVLQKEFWSDLAEYGYSTLSGVPYFYELLHRFGFFKKDTPS
EHHHEECCEECCEEEECCHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHCCCCCCCCC
VRYFTHTGGMLNQELANVISNYTNIYDKQFFAQYGQTEASGRMAYLPPKDFETKGTSIGF
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCEECC
PVKNGRFEIDENTGELIYYGPNVSGGYANNIFDLQHYDQTDKLYTGDLAEKDELGYYHIK
CCCCCEEEEECCCCCEEEECCCCCCCCCCCEEECCCCCCCCCEEECCCCCCCCCCEEEHH
GRIKRIMKLFGVRLNLDEVEVLLKNQLGGQTFICISVQDKYLGVIHQDASLLRETITQVI
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCEEEEHHHHHHHHHHHHHHH
KEKLGLHASSIKNYYFDQVPLTVNGKVDYPAINKYINENENVATAAKTVVG
HHHHCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]