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The map label for this gene is lacZ [H]

Identifier: 146300856

GI number: 146300856

Start: 3680580

End: 3683000

Strand: Reverse

Name: lacZ [H]

Synonym: Fjoh_3111

Alternate gene names: 146300856

Gene position: 3683000-3680580 (Counterclockwise)

Preceding gene: 146300857

Following gene: 146300855

Centisome position: 60.41

GC content: 38.46

Gene sequence:

>2421_bases
GTGTTTAAAAATAAGATCTTACTGCCCGTACTTTTTCTTTCGTGTTTTTCTGCATTTGCTCAAATCTCGTTTGGAGATGC
AAAGAAAATAAATGACAATTGGAAGTTTAACCTTCAGGATGTTTCTGATGCTAAAAACGTCACTTTTGACGACAGCAAAT
GGAAACAAGTAAACGTACCGCACGACTGGAGTGTAAAAGGACAATTGAGCCCGACTTTGGCAAGCTGTACAGGTTATCTG
CCAGGAGGAATTGCGTGGTACAGAAAATCCATAAATATTCCGCAGAGCAAAGCAGGCGAAAAAGTGTATTTGTATTTTGA
AGGCGTTTACAATAGAAGCGAAGTTTTCATCAACGGGCATTCGTTAGGAAAACGTCCAAACGGATATATTTCGTTTGCTT
ATGATGCTACAGAATTCGTGAAATTTGGAAACGAAAACACTATTTCGGTTCGTGTAGATCATAGTCAAAGCGCCGATTCA
CGATGGTACAGCGGTTCTGGAATTTACAGAAATGTCTGGTTGGTTTATGCAAATCCGATTCATATTGCACAATGGGGCGT
TTATGCCTATCCGGAAGTACAAAATGGAAATGGAGTTTTGAATGTTGAAGTAGAGGTAGAAAATAATGTTGCATCAAAAA
CGGCTTTAACCGTTGTAAATGAATTATTTTTTAAAGGAAAATCCATTGCAAAATCTTCTTCAAAAACAACCATTTTTGCT
AATACTAAAGGAAAAATTGCAGCAAAAATTAACGTTAAGAACCCGAAGTTATGGGATTTAGAAAATCCGAATTTGTATCA
GCTGAAAACTACGGTTTTACAAAACGGAAAAGAAATCGATAAAACGGAAACACAGACTGGTTTTAGAAGTTTTACGTTCG
ATGCCAATAATGGTTTTGCCCTTAACGGAAAATGGATGAAAATGAAAGGCGTTTGCCTGCATCACGATGCCGGTGTTTTA
GGATCGGCAGTTCCAAGAGAAGTTTGGAAAACCAGATTAGGAACGTTAAAAGAAATCGGAGTAAATGCCATTCGTACCAG
TCATAATCCGCAGGCGCCGGATTTCTATGAACTTTGTGATGAATTAGGTTTATTGGTTTTGAATGAAGCTTATGACGAAT
GGGAATTCCCAAAACGCAAATGGTTGCAAGGCTGGAATTACGGTACGCCGGGTTACGAAGGTTCGTTTGATATTTTTGCC
GATTGGGCAGAAAAGGATTTGGAAGATTTTGTGCGACGCGACAGAAACCATGTTTCGGTTTTTGCATGGAGTATTGGCAA
CGAAGTAGATTATCCAAACGATCCGTATTCGCATCCCGTTTTAGACAAAGGAAAAGACGGTTTTGGACAGGCAACTTATG
GCGGTTACAAAAAAGATGCTCCCGATGCGATGCGTCTTGGAGCAATTGCCAAACGTTTGGTTGCAGCAGTTAAGAAATAT
GATAAATCACGTCCGACAACGGCAGGTTTGGCTGGAGTAGCCATGTCTAATGAAACAGAATATCCGGGAGCTTTGGATAT
TACAGGATACAATTATACAGAAAGTAAATACCAATCAGACCACGAAAAATATCCAAACCGAGTTATCTACGGAAGTGAAA
ACACACACGACATGCAGCCTTGGCTTTCGGTTAAAAACAACAAACATATTTTTGGGCAGTTTTTATGGACAGGAATTGAT
TATCTGGGAGAATCGGGAAGATGGCCCTCTAGAGGATTTTATTCTGGTTTAGTTGATTTTGCAGGCGTTATCAAACCGCG
AGGGTATTTCCGCCAATCTTTATGGTCAGATAAACCGATGATTTATTTGGGAACTTATCCGCTTGTAAATGAAAAAGATA
TTTCTAAAGATGCCTGGTCAATCTGGAATTACGAAGAAGGACAAAAAATACGCGTGGTTTGTTATACCAATGCCTCAAAA
GCACGTTTAGAATTGAATGGAAAAGTAGTCGGCGAAACGAAATTATATGATGAAAAAACCGGCATACTGTATTGGGATAT
TCCCTTTACATCTGGAAAATTAGAAGCAGTTGGATTGGATAAAAATGATAAAGAAGTAAGCCGTTATGGGATTAATTCAA
CACAACAGCCTTTTGAATTAACGATTGCAGATAAAAATAGTACCATCAGCAAAGATAAAGGGGTTGCGAAAATCATGGTT
CAGGTAAAAGACCAAAACGGACTTCCGGTTATGCTTTCAGACAATGAAGTAACTTGTACGATTTCAGGTTCTGGAACTTT
ATTAGGTTTAGAAGCTGGAAACAACAGTGATATGACCGATTATACAGATAATGTTCAAAGAGTTTTTCACGGACATATTG
CGGCTTATATTCAGGCTAATGGAGATTCGACAGAGCCTATAAAAGTAAAATTTACGAGTCAATGGTTAAAACCTGTAGAA
GTTACTATTAATGTCAAATAA

Upstream 100 bases:

>100_bases
TCTATTGGATATACGGCTGTATTTGAGACCAAAAAAGGCACATCTTACATCATAAATCCTCTTCAATAATTTTTCATTTA
ATAAACTAAAAAATACCATC

Downstream 100 bases:

>100_bases
TTTAAAATAATGTGACATACCGCTGCGCTGGAGCTTTTCATACAAAACAGATAAAATTAGCTATACATATAACGCTCTTC
CAGAGCTTAATGTCTCATCG

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 806; Mature: 806

Protein sequence:

>806_residues
MFKNKILLPVLFLSCFSAFAQISFGDAKKINDNWKFNLQDVSDAKNVTFDDSKWKQVNVPHDWSVKGQLSPTLASCTGYL
PGGIAWYRKSINIPQSKAGEKVYLYFEGVYNRSEVFINGHSLGKRPNGYISFAYDATEFVKFGNENTISVRVDHSQSADS
RWYSGSGIYRNVWLVYANPIHIAQWGVYAYPEVQNGNGVLNVEVEVENNVASKTALTVVNELFFKGKSIAKSSSKTTIFA
NTKGKIAAKINVKNPKLWDLENPNLYQLKTTVLQNGKEIDKTETQTGFRSFTFDANNGFALNGKWMKMKGVCLHHDAGVL
GSAVPREVWKTRLGTLKEIGVNAIRTSHNPQAPDFYELCDELGLLVLNEAYDEWEFPKRKWLQGWNYGTPGYEGSFDIFA
DWAEKDLEDFVRRDRNHVSVFAWSIGNEVDYPNDPYSHPVLDKGKDGFGQATYGGYKKDAPDAMRLGAIAKRLVAAVKKY
DKSRPTTAGLAGVAMSNETEYPGALDITGYNYTESKYQSDHEKYPNRVIYGSENTHDMQPWLSVKNNKHIFGQFLWTGID
YLGESGRWPSRGFYSGLVDFAGVIKPRGYFRQSLWSDKPMIYLGTYPLVNEKDISKDAWSIWNYEEGQKIRVVCYTNASK
ARLELNGKVVGETKLYDEKTGILYWDIPFTSGKLEAVGLDKNDKEVSRYGINSTQQPFELTIADKNSTISKDKGVAKIMV
QVKDQNGLPVMLSDNEVTCTISGSGTLLGLEAGNNSDMTDYTDNVQRVFHGHIAAYIQANGDSTEPIKVKFTSQWLKPVE
VTINVK

Sequences:

>Translated_806_residues
MFKNKILLPVLFLSCFSAFAQISFGDAKKINDNWKFNLQDVSDAKNVTFDDSKWKQVNVPHDWSVKGQLSPTLASCTGYL
PGGIAWYRKSINIPQSKAGEKVYLYFEGVYNRSEVFINGHSLGKRPNGYISFAYDATEFVKFGNENTISVRVDHSQSADS
RWYSGSGIYRNVWLVYANPIHIAQWGVYAYPEVQNGNGVLNVEVEVENNVASKTALTVVNELFFKGKSIAKSSSKTTIFA
NTKGKIAAKINVKNPKLWDLENPNLYQLKTTVLQNGKEIDKTETQTGFRSFTFDANNGFALNGKWMKMKGVCLHHDAGVL
GSAVPREVWKTRLGTLKEIGVNAIRTSHNPQAPDFYELCDELGLLVLNEAYDEWEFPKRKWLQGWNYGTPGYEGSFDIFA
DWAEKDLEDFVRRDRNHVSVFAWSIGNEVDYPNDPYSHPVLDKGKDGFGQATYGGYKKDAPDAMRLGAIAKRLVAAVKKY
DKSRPTTAGLAGVAMSNETEYPGALDITGYNYTESKYQSDHEKYPNRVIYGSENTHDMQPWLSVKNNKHIFGQFLWTGID
YLGESGRWPSRGFYSGLVDFAGVIKPRGYFRQSLWSDKPMIYLGTYPLVNEKDISKDAWSIWNYEEGQKIRVVCYTNASK
ARLELNGKVVGETKLYDEKTGILYWDIPFTSGKLEAVGLDKNDKEVSRYGINSTQQPFELTIADKNSTISKDKGVAKIMV
QVKDQNGLPVMLSDNEVTCTISGSGTLLGLEAGNNSDMTDYTDNVQRVFHGHIAAYIQANGDSTEPIKVKFTSQWLKPVE
VTINVK
>Mature_806_residues
MFKNKILLPVLFLSCFSAFAQISFGDAKKINDNWKFNLQDVSDAKNVTFDDSKWKQVNVPHDWSVKGQLSPTLASCTGYL
PGGIAWYRKSINIPQSKAGEKVYLYFEGVYNRSEVFINGHSLGKRPNGYISFAYDATEFVKFGNENTISVRVDHSQSADS
RWYSGSGIYRNVWLVYANPIHIAQWGVYAYPEVQNGNGVLNVEVEVENNVASKTALTVVNELFFKGKSIAKSSSKTTIFA
NTKGKIAAKINVKNPKLWDLENPNLYQLKTTVLQNGKEIDKTETQTGFRSFTFDANNGFALNGKWMKMKGVCLHHDAGVL
GSAVPREVWKTRLGTLKEIGVNAIRTSHNPQAPDFYELCDELGLLVLNEAYDEWEFPKRKWLQGWNYGTPGYEGSFDIFA
DWAEKDLEDFVRRDRNHVSVFAWSIGNEVDYPNDPYSHPVLDKGKDGFGQATYGGYKKDAPDAMRLGAIAKRLVAAVKKY
DKSRPTTAGLAGVAMSNETEYPGALDITGYNYTESKYQSDHEKYPNRVIYGSENTHDMQPWLSVKNNKHIFGQFLWTGID
YLGESGRWPSRGFYSGLVDFAGVIKPRGYFRQSLWSDKPMIYLGTYPLVNEKDISKDAWSIWNYEEGQKIRVVCYTNASK
ARLELNGKVVGETKLYDEKTGILYWDIPFTSGKLEAVGLDKNDKEVSRYGINSTQQPFELTIADKNSTISKDKGVAKIMV
QVKDQNGLPVMLSDNEVTCTISGSGTLLGLEAGNNSDMTDYTDNVQRVFHGHIAAYIQANGDSTEPIKVKFTSQWLKPVE
VTINVK

Specific function: The Wild-Type Enzyme Is An Ineffective Lactase. Two Classes Of Point Mutations Dramatically Improve Activity Of The Enzyme. [C]

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=441, Percent_Identity=21.7687074829932, Blast_Score=77, Evalue=5e-14,
Organism=Escherichia coli, GI48994920, Length=397, Percent_Identity=25.1889168765743, Blast_Score=129, Evalue=7e-31,
Organism=Escherichia coli, GI1786539, Length=432, Percent_Identity=26.1574074074074, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1787903, Length=438, Percent_Identity=24.4292237442922, Blast_Score=97, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17510825, Length=377, Percent_Identity=25.9946949602122, Blast_Score=93, Evalue=6e-19,
Organism=Drosophila melanogaster, GI62471735, Length=480, Percent_Identity=25.4166666666667, Blast_Score=105, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19922582, Length=480, Percent_Identity=25.4166666666667, Blast_Score=105, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24655438, Length=480, Percent_Identity=25.4166666666667, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24651745, Length=478, Percent_Identity=24.2677824267782, Blast_Score=102, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 90594; Mature: 90594

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKNKILLPVLFLSCFSAFAQISFGDAKKINDNWKFNLQDVSDAKNVTFDDSKWKQVNVP
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCEECCCCCEEECCC
HDWSVKGQLSPTLASCTGYLPGGIAWYRKSINIPQSKAGEKVYLYFEGVYNRSEVFINGH
CCCCCCCCCCCCHHHHCCCCCCCHHHHHHCCCCCCCCCCCEEEEEEECCCCCCEEEEECC
SLGKRPNGYISFAYDATEFVKFGNENTISVRVDHSQSADSRWYSGSGIYRNVWLVYANPI
CCCCCCCCCEEEEECHHHHHHCCCCCEEEEEEECCCCCCCCEECCCCCEEEEEEEEECCE
HIAQWGVYAYPEVQNGNGVLNVEVEVENNVASKTALTVVNELFFKGKSIAKSSSKTTIFA
EEEECCEEECCEEECCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCHHCCCCCCEEEEE
NTKGKIAAKINVKNPKLWDLENPNLYQLKTTVLQNGKEIDKTETQTGFRSFTFDANNGFA
ECCCEEEEEEEECCCEEEECCCCCEEEEEHHHHHCCCCCCCHHHCCCCEEEEEECCCCEE
LNGKWMKMKGVCLHHDAGVLGSAVPREVWKTRLGTLKEIGVNAIRTSHNPQAPDFYELCD
ECCEEEEEECEEEECCCCCCCCHHHHHHHHHHHCCHHHHCCCEEECCCCCCCCCHHHHHH
ELGLLVLNEAYDEWEFPKRKWLQGWNYGTPGYEGSFDIFADWAEKDLEDFVRRDRNHVSV
HCCEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCHHEEECHHHHHHHHHHHCCCCEEEE
FAWSIGNEVDYPNDPYSHPVLDKGKDGFGQATYGGYKKDAPDAMRLGAIAKRLVAAVKKY
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
DKSRPTTAGLAGVAMSNETEYPGALDITGYNYTESKYQSDHEKYPNRVIYGSENTHDMQP
CCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCHHHHCCHHHHCCCEEEECCCCCCCCCC
WLSVKNNKHIFGQFLWTGIDYLGESGRWPSRGFYSGLVDFAGVIKPRGYFRQSLWSDKPM
CEECCCCCEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCE
IYLGTYPLVNEKDISKDAWSIWNYEEGQKIRVVCYTNASKARLELNGKVVGETKLYDEKT
EEEECCCCCCCCCCCCCHHCCCCCCCCCEEEEEEEECCCCEEEEECCEEEECEEEEECCC
GILYWDIPFTSGKLEAVGLDKNDKEVSRYGINSTQQPFELTIADKNSTISKDKGVAKIMV
CEEEEECCCCCCCEEEEECCCCCHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEEE
QVKDQNGLPVMLSDNEVTCTISGSGTLLGLEAGNNSDMTDYTDNVQRVFHGHIAAYIQAN
EEECCCCCEEEECCCEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHCEEEEEEEEC
GDSTEPIKVKFTSQWLKPVEVTINVK
CCCCCCEEEEEECCCCCEEEEEEEEC
>Mature Secondary Structure
MFKNKILLPVLFLSCFSAFAQISFGDAKKINDNWKFNLQDVSDAKNVTFDDSKWKQVNVP
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCEECCCCCEEECCC
HDWSVKGQLSPTLASCTGYLPGGIAWYRKSINIPQSKAGEKVYLYFEGVYNRSEVFINGH
CCCCCCCCCCCCHHHHCCCCCCCHHHHHHCCCCCCCCCCCEEEEEEECCCCCCEEEEECC
SLGKRPNGYISFAYDATEFVKFGNENTISVRVDHSQSADSRWYSGSGIYRNVWLVYANPI
CCCCCCCCCEEEEECHHHHHHCCCCCEEEEEEECCCCCCCCEECCCCCEEEEEEEEECCE
HIAQWGVYAYPEVQNGNGVLNVEVEVENNVASKTALTVVNELFFKGKSIAKSSSKTTIFA
EEEECCEEECCEEECCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCHHCCCCCCEEEEE
NTKGKIAAKINVKNPKLWDLENPNLYQLKTTVLQNGKEIDKTETQTGFRSFTFDANNGFA
ECCCEEEEEEEECCCEEEECCCCCEEEEEHHHHHCCCCCCCHHHCCCCEEEEEECCCCEE
LNGKWMKMKGVCLHHDAGVLGSAVPREVWKTRLGTLKEIGVNAIRTSHNPQAPDFYELCD
ECCEEEEEECEEEECCCCCCCCHHHHHHHHHHHCCHHHHCCCEEECCCCCCCCCHHHHHH
ELGLLVLNEAYDEWEFPKRKWLQGWNYGTPGYEGSFDIFADWAEKDLEDFVRRDRNHVSV
HCCEEEEECCCCCCCCCHHHHHCCCCCCCCCCCCCHHEEECHHHHHHHHHHHCCCCEEEE
FAWSIGNEVDYPNDPYSHPVLDKGKDGFGQATYGGYKKDAPDAMRLGAIAKRLVAAVKKY
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
DKSRPTTAGLAGVAMSNETEYPGALDITGYNYTESKYQSDHEKYPNRVIYGSENTHDMQP
CCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCHHHHCCHHHHCCCEEEECCCCCCCCCC
WLSVKNNKHIFGQFLWTGIDYLGESGRWPSRGFYSGLVDFAGVIKPRGYFRQSLWSDKPM
CEECCCCCEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCE
IYLGTYPLVNEKDISKDAWSIWNYEEGQKIRVVCYTNASKARLELNGKVVGETKLYDEKT
EEEECCCCCCCCCCCCCHHCCCCCCCCCEEEEEEEECCCCEEEEECCEEEECEEEEECCC
GILYWDIPFTSGKLEAVGLDKNDKEVSRYGINSTQQPFELTIADKNSTISKDKGVAKIMV
CEEEEECCCCCCCEEEEECCCCCHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEEE
QVKDQNGLPVMLSDNEVTCTISGSGTLLGLEAGNNSDMTDYTDNVQRVFHGHIAAYIQAN
EEECCCCCEEEECCCEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHCEEEEEEEEC
GDSTEPIKVKFTSQWLKPVEVTINVK
CCCCCCEEEEEECCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA