Click here to switch to the map view.

The map label for this gene is agaA [H]

Identifier: 146300855

GI number: 146300855

Start: 3679098

End: 3680459

Strand: Reverse

Name: agaA [H]

Synonym: Fjoh_3110

Alternate gene names: 146300855

Gene position: 3680459-3679098 (Counterclockwise)

Preceding gene: 146300856

Following gene: 146300854

Centisome position: 60.37

GC content: 37.89

Gene sequence:

>1362_bases
ATGAAAAATAAAATCACATTGGTGTTAGGAATCGTTTTGGTTTCGATTTCTTCACATTCGCAGTATAAAACATTTCAAAA
TGAAGAATTCAAACAATGGGCACAAACACCGCCAATGGGCTGGAACAGCTGGGATTGTTACGGGCCGACAGTCGAAGAAC
ACGAGGTTAAAACCAACGCCGATTATATGGCAAAAGAGCTTAAGAAATTCGGCTGGGAATATGTAGTAGTTGATATTAGA
TGGTTTGTCGAAAATGATAAAGCGGGAGGTTACAACCAAACCGATCCGAGATATGTAATGGATGAGTACGGAAGATATAT
GCCAGCTGTAAACCGTTTCCCATCTGCCAAAGACGGACAGGGTTTTAAGCCTTTAGCCGATTATATTCATAAAAAAGGAT
TGAAATTCGGAATCCATATCATGAGAGGGATTCCTAAAAAAGCAGTCGAAAACAAACTGCCAATAAAAGGAACAAACGGA
ATTACCGCCGACCAAGTTTATTCTACCGCTTTGCAATGTGAATGGTTACGAGATAATTATACTGTTTTGGCAGACAAACC
AGGCGCACAGGAATATTACAATTCTATTTTTGAATTGTATGCAAAATGGGGTGTAGATTTTATTAAAATTGATGATTTAT
CACGACCTTATCATGAAGGCGAAATCAACTTAATTCGAAACGCCATTGACAAATGCGGCCGTAAAATTGTCTTGAGTACT
TCGCCGGGAGAAACGCCGATTTCGGCTGCTTCGCACGTAAGCACGCATGCTAATATGTGGCGTATGGTTGACGATGTTTG
GGATACATGGCCGCATATCACACATTTAATGGATGTGGCTCAAAAATGGTATCCGTATATCGCTCCGGGAACTTGGCCGG
ATTGTGATATGATTCCGTTAGGAAGAATTTCAATCAGAGGCGAACGCGGAAAAGATCGAATGACGCGTTTGACAAAAGAC
GAACAATATACTTTAATCACGTTTTTCAATATTTTTAAATCACCATTATTTTTTGGTGGTGATTTGCCCAGCAATGATGC
TTTTACGCTTTCATTATTAACAAATAAAGAAGTTGTAAAAATGCATAACGAAAGCACAGATGTAAAAGAGCTTTTTCAGA
AAGATGGGAAAATTGCTGTTACTTCAAAAGCAAAAGATGGAAGTGTCTATCTCGCATTATTTAATATCGCTGATAACGCT
TCTCAAAAAGTCTCTGTAAATCTTTCAGATCTTGGAATTTCTGGTTCTGTTGATGTTTTAAATATGTGGACAGGAGAAAA
ATCAAAAGTGACTTCAACAGAAATTGGTACCGATTTAAAACCGCACAGTTCTGTTTTGTACCAATTAAAAAGTAAAAAAT
AA

Upstream 100 bases:

>100_bases
CGCTGCGCTGGAGCTTTTCATACAAAACAGATAAAATTAGCTATACATATAACGCTCTTCCAGAGCTTAATGTCTCATCG
GGACAATTTATTGGTATCTT

Downstream 100 bases:

>100_bases
TGGAAAGATTGGTTTTCTATTTTCTGATATTAATGACTGTTTTTAGTCCGGCGAAAGCTCAAAATACGGCAAGTGGAATG
CCGCCTGTTCTTGCTGAAAA

Product: hypothetical protein

Products: NA

Alternate protein names: Alpha-D-galactoside galactohydrolase; Alpha-galactosidase 27A; Melibiase [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MKNKITLVLGIVLVSISSHSQYKTFQNEEFKQWAQTPPMGWNSWDCYGPTVEEHEVKTNADYMAKELKKFGWEYVVVDIR
WFVENDKAGGYNQTDPRYVMDEYGRYMPAVNRFPSAKDGQGFKPLADYIHKKGLKFGIHIMRGIPKKAVENKLPIKGTNG
ITADQVYSTALQCEWLRDNYTVLADKPGAQEYYNSIFELYAKWGVDFIKIDDLSRPYHEGEINLIRNAIDKCGRKIVLST
SPGETPISAASHVSTHANMWRMVDDVWDTWPHITHLMDVAQKWYPYIAPGTWPDCDMIPLGRISIRGERGKDRMTRLTKD
EQYTLITFFNIFKSPLFFGGDLPSNDAFTLSLLTNKEVVKMHNESTDVKELFQKDGKIAVTSKAKDGSVYLALFNIADNA
SQKVSVNLSDLGISGSVDVLNMWTGEKSKVTSTEIGTDLKPHSSVLYQLKSKK

Sequences:

>Translated_453_residues
MKNKITLVLGIVLVSISSHSQYKTFQNEEFKQWAQTPPMGWNSWDCYGPTVEEHEVKTNADYMAKELKKFGWEYVVVDIR
WFVENDKAGGYNQTDPRYVMDEYGRYMPAVNRFPSAKDGQGFKPLADYIHKKGLKFGIHIMRGIPKKAVENKLPIKGTNG
ITADQVYSTALQCEWLRDNYTVLADKPGAQEYYNSIFELYAKWGVDFIKIDDLSRPYHEGEINLIRNAIDKCGRKIVLST
SPGETPISAASHVSTHANMWRMVDDVWDTWPHITHLMDVAQKWYPYIAPGTWPDCDMIPLGRISIRGERGKDRMTRLTKD
EQYTLITFFNIFKSPLFFGGDLPSNDAFTLSLLTNKEVVKMHNESTDVKELFQKDGKIAVTSKAKDGSVYLALFNIADNA
SQKVSVNLSDLGISGSVDVLNMWTGEKSKVTSTEIGTDLKPHSSVLYQLKSKK
>Mature_453_residues
MKNKITLVLGIVLVSISSHSQYKTFQNEEFKQWAQTPPMGWNSWDCYGPTVEEHEVKTNADYMAKELKKFGWEYVVVDIR
WFVENDKAGGYNQTDPRYVMDEYGRYMPAVNRFPSAKDGQGFKPLADYIHKKGLKFGIHIMRGIPKKAVENKLPIKGTNG
ITADQVYSTALQCEWLRDNYTVLADKPGAQEYYNSIFELYAKWGVDFIKIDDLSRPYHEGEINLIRNAIDKCGRKIVLST
SPGETPISAASHVSTHANMWRMVDDVWDTWPHITHLMDVAQKWYPYIAPGTWPDCDMIPLGRISIRGERGKDRMTRLTKD
EQYTLITFFNIFKSPLFFGGDLPSNDAFTLSLLTNKEVVKMHNESTDVKELFQKDGKIAVTSKAKDGSVYLALFNIADNA
SQKVSVNLSDLGISGSVDVLNMWTGEKSKVTSTEIGTDLKPHSSVLYQLKSKK

Specific function: Hydrolyzes galactomannan found in plant cell wall, by cleaving alpha-1,6-D-galactose side-chains from the mannan backbone. Appears to act in synergy with mannanase (ManA) to elicit hydrolysis of galactomannan. Has greater activity against galactomannans w

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 27 family [H]

Homologues:

Organism=Drosophila melanogaster, GI24583253, Length=412, Percent_Identity=24.7572815533981, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013780
- InterPro:   IPR002241
- InterPro:   IPR017853
- InterPro:   IPR000111 [H]

Pfam domain/function: PF02065 Melibiase [H]

EC number: =3.2.1.22 [H]

Molecular weight: Translated: 51477; Mature: 51477

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKITLVLGIVLVSISSHSQYKTFQNEEFKQWAQTPPMGWNSWDCYGPTVEEHEVKTNA
CCCCEEEHEEEHHHHCCCCCHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCH
DYMAKELKKFGWEYVVVDIRWFVENDKAGGYNQTDPRYVMDEYGRYMPAVNRFPSAKDGQ
HHHHHHHHHCCCEEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCCCCCC
GFKPLADYIHKKGLKFGIHIMRGIPKKAVENKLPIKGTNGITADQVYSTALQCEWLRDNY
CCCHHHHHHHHCCCHHHHHHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHEEEEECCCE
TVLADKPGAQEYYNSIFELYAKWGVDFIKIDDLSRPYHEGEINLIRNAIDKCGRKIVLST
EEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEEC
SPGETPISAASHVSTHANMWRMVDDVWDTWPHITHLMDVAQKWYPYIAPGTWPDCDMIPL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCC
GRISIRGERGKDRMTRLTKDEQYTLITFFNIFKSPLFFGGDLPSNDAFTLSLLTNKEVVK
CCEEEECCCCHHHHHHCCCCCCEEEEEHHHHHHCCCEECCCCCCCCCEEEEEECCCHHEE
MHNESTDVKELFQKDGKIAVTSKAKDGSVYLALFNIADNASQKVSVNLSDLGISGSVDVL
CCCCCCHHHHHHHCCCCEEEEECCCCCEEEEEEEEECCCCCCEEEEEHHHCCCCCCCEEE
NMWTGEKSKVTSTEIGTDLKPHSSVLYQLKSKK
EECCCCCCCCCHHHCCCCCCCCHHHHHHCCCCC
>Mature Secondary Structure
MKNKITLVLGIVLVSISSHSQYKTFQNEEFKQWAQTPPMGWNSWDCYGPTVEEHEVKTNA
CCCCEEEHEEEHHHHCCCCCHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCH
DYMAKELKKFGWEYVVVDIRWFVENDKAGGYNQTDPRYVMDEYGRYMPAVNRFPSAKDGQ
HHHHHHHHHCCCEEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCCCCCC
GFKPLADYIHKKGLKFGIHIMRGIPKKAVENKLPIKGTNGITADQVYSTALQCEWLRDNY
CCCHHHHHHHHCCCHHHHHHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHEEEEECCCE
TVLADKPGAQEYYNSIFELYAKWGVDFIKIDDLSRPYHEGEINLIRNAIDKCGRKIVLST
EEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEEC
SPGETPISAASHVSTHANMWRMVDDVWDTWPHITHLMDVAQKWYPYIAPGTWPDCDMIPL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCC
GRISIRGERGKDRMTRLTKDEQYTLITFFNIFKSPLFFGGDLPSNDAFTLSLLTNKEVVK
CCEEEECCCCHHHHHHCCCCCCEEEEEHHHHHHCCCEECCCCCCCCCEEEEEECCCHHEE
MHNESTDVKELFQKDGKIAVTSKAKDGSVYLALFNIADNASQKVSVNLSDLGISGSVDVL
CCCCCCHHHHHHHCCCCEEEEECCCCCEEEEEEEEECCCCCCEEEEEHHHCCCCCCCEEE
NMWTGEKSKVTSTEIGTDLKPHSSVLYQLKSKK
EECCCCCCCCCHHHCCCCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA