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The map label for this gene is qor [C]

Identifier: 146300846

GI number: 146300846

Start: 3669948

End: 3670949

Strand: Direct

Name: qor [C]

Synonym: Fjoh_3101

Alternate gene names: 146300846

Gene position: 3669948-3670949 (Clockwise)

Preceding gene: 146300845

Following gene: 146300848

Centisome position: 60.19

GC content: 35.63

Gene sequence:

>1002_bases
ATGAAAGCATACATCTTCCCTAAATATGGCAAAACAGAAAAATTATTACTCAGCGATGTAAAAATACCTGAAATTGGAGA
TTATGAGGTATTAGTAGAAATTCATGCTGCCAGCATCAACCAGCTGGACTTAAAAATAAAAAATGGGGATTTCAAGTTAA
TCCTTCATTACCAATTACCTTTAATATTAGGTCATGATATTGCAGGAATAGTAGTGGAAACTGGCTCAAAAGTCAGCCGC
TTTAAAATTGGAGACCAGATTTATGGACGAGCAGCCGATTTTCATATCGGTTCCTTCGCGGAATATATTGCTGTTAATGA
AAATGATCTGGCAGTAAAACCTATAAATATATCAATGGAGCAGGCAGCATCAATTCCATTGGTCGGACTAACTGTATGGC
AGGCATTCGTCGAAAAAGCGAAACTTCGTAAAGGCCAAAAAGTTTTTATTCAAGCAGGTTCTGGCGGAGTTGGCACAATA
GCAATTCAGTTTGCCAAACATCTTGGGGCATTTGTCGCCACGACAGCCAGCGAAAAGAATTTTGACATGCTGAAAGCCCT
TGGAGCTGATATTATAATAGATTACAAAAAGGATAACTTTGAAAATATTCTTAAAGATTATGATGTGGTACTTAATAGCC
AGGATGTTAAAACACTTGAAAAATCATTGACAATTTTAAAACCGGGAGGCAGTATTATCTCAATTTCTGGACCTCCAACT
CCAGAGTTTGCAAAATCAATAGGCGCATCAGCAATAATACGCTTCATTATTTGGCTGACCAGCCTAAAAATCCGTAATAG
GGCTAAAAATTTAAATATTAATTATTCCTTCTTATTCATGAAGGCGAACGGAAGACAACTCGAACAAATAAATAATTTAC
TAGAATCTAAAGCAATTAAACCAGTTGTGGATAAAGTATTTTCTTTTGAACAAACCAACGATGCTGTTGAATATATGGAA
AAAGGACATGCAAAAGGAAAAGTGGTTGTAAAAATAAAATGA

Upstream 100 bases:

>100_bases
GAGGAGCCGGAAGCGCGCAATCGTATTAAGAATCTATTTGAAAGAGGACTCCAGCAAAATGGCGATCTGGAACTAAATCT
TGCTAATAACATCTAAAAAT

Downstream 100 bases:

>100_bases
TCACAAACTTCTTTTTTAATTGTCATCAATAAATTTTGACCCAAATATTTTCCTAATTATTATTTAAATACTTCAATACT
TTGAATTTTTGACGACAAAA

Product: alcohol dehydrogenase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MKAYIFPKYGKTEKLLLSDVKIPEIGDYEVLVEIHAASINQLDLKIKNGDFKLILHYQLPLILGHDIAGIVVETGSKVSR
FKIGDQIYGRAADFHIGSFAEYIAVNENDLAVKPINISMEQAASIPLVGLTVWQAFVEKAKLRKGQKVFIQAGSGGVGTI
AIQFAKHLGAFVATTASEKNFDMLKALGADIIIDYKKDNFENILKDYDVVLNSQDVKTLEKSLTILKPGGSIISISGPPT
PEFAKSIGASAIIRFIIWLTSLKIRNRAKNLNINYSFLFMKANGRQLEQINNLLESKAIKPVVDKVFSFEQTNDAVEYME
KGHAKGKVVVKIK

Sequences:

>Translated_333_residues
MKAYIFPKYGKTEKLLLSDVKIPEIGDYEVLVEIHAASINQLDLKIKNGDFKLILHYQLPLILGHDIAGIVVETGSKVSR
FKIGDQIYGRAADFHIGSFAEYIAVNENDLAVKPINISMEQAASIPLVGLTVWQAFVEKAKLRKGQKVFIQAGSGGVGTI
AIQFAKHLGAFVATTASEKNFDMLKALGADIIIDYKKDNFENILKDYDVVLNSQDVKTLEKSLTILKPGGSIISISGPPT
PEFAKSIGASAIIRFIIWLTSLKIRNRAKNLNINYSFLFMKANGRQLEQINNLLESKAIKPVVDKVFSFEQTNDAVEYME
KGHAKGKVVVKIK
>Mature_333_residues
MKAYIFPKYGKTEKLLLSDVKIPEIGDYEVLVEIHAASINQLDLKIKNGDFKLILHYQLPLILGHDIAGIVVETGSKVSR
FKIGDQIYGRAADFHIGSFAEYIAVNENDLAVKPINISMEQAASIPLVGLTVWQAFVEKAKLRKGQKVFIQAGSGGVGTI
AIQFAKHLGAFVATTASEKNFDMLKALGADIIIDYKKDNFENILKDYDVVLNSQDVKTLEKSLTILKPGGSIISISGPPT
PEFAKSIGASAIIRFIIWLTSLKIRNRAKNLNINYSFLFMKANGRQLEQINNLLESKAIKPVVDKVFSFEQTNDAVEYME
KGHAKGKVVVKIK

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI47519420, Length=355, Percent_Identity=30.7042253521127, Blast_Score=175, Evalue=6e-44,
Organism=Homo sapiens, GI22538446, Length=352, Percent_Identity=29.2613636363636, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI22538444, Length=352, Percent_Identity=29.2613636363636, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI194239674, Length=250, Percent_Identity=27.2, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI13236495, Length=250, Percent_Identity=27.2, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI194239676, Length=229, Percent_Identity=26.6375545851528, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI24308257, Length=350, Percent_Identity=26.2857142857143, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI18379349, Length=317, Percent_Identity=23.9747634069401, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI28557745, Length=339, Percent_Identity=25.6637168141593, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI90669511, Length=163, Percent_Identity=31.2883435582822, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1790485, Length=238, Percent_Identity=33.1932773109244, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1790045, Length=261, Percent_Identity=26.8199233716475, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71987554, Length=371, Percent_Identity=27.4932614555256, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17507255, Length=343, Percent_Identity=25.3644314868805, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17556000, Length=249, Percent_Identity=29.3172690763052, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17562584, Length=218, Percent_Identity=29.3577981651376, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319621, Length=300, Percent_Identity=28.3333333333333, Blast_Score=96, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6323729, Length=364, Percent_Identity=26.0989010989011, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324486, Length=362, Percent_Identity=25.6906077348066, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6323961, Length=362, Percent_Identity=25.9668508287293, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324195, Length=303, Percent_Identity=27.7227722772277, Blast_Score=76, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6319520, Length=228, Percent_Identity=28.9473684210526, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24581339, Length=366, Percent_Identity=30.0546448087432, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24581341, Length=366, Percent_Identity=30.0546448087432, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24581345, Length=253, Percent_Identity=28.4584980237154, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR014182
- InterPro:   IPR011032
- InterPro:   IPR016040
- InterPro:   IPR002364 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 36798; Mature: 36798

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAYIFPKYGKTEKLLLSDVKIPEIGDYEVLVEIHAASINQLDLKIKNGDFKLILHYQLP
CCEEEECCCCCCCCEEEECCCCCCCCCEEEEEEEEECCCEEEEEEEECCCEEEEEEEECC
LILGHDIAGIVVETGSKVSRFKIGDQIYGRAADFHIGSFAEYIAVNENDLAVKPINISME
EEECCCCEEEEEECCCCEEEEECCHHHHCCCCCCCCCCCEEEEEECCCCEEEEEECCCHH
QAASIPLVGLTVWQAFVEKAKLRKGQKVFIQAGSGGVGTIAIQFAKHLGAFVATTASEKN
HHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEHHHHHHHHHHHHHEEECCCCCC
FDMLKALGADIIIDYKKDNFENILKDYDVVLNSQDVKTLEKSLTILKPGGSIISISGPPT
HHHHHHCCCCEEEEECCCCHHHHHHHHHHEECCCHHHHHHHCEEEECCCCCEEEECCCCC
PEFAKSIGASAIIRFIIWLTSLKIRNRAKNLNINYSFLFMKANGRQLEQINNLLESKAIK
HHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHCHH
PVVDKVFSFEQTNDAVEYMEKGHAKGKVVVKIK
HHHHHHHCCCCCCHHHHHHHCCCCCCEEEEEEC
>Mature Secondary Structure
MKAYIFPKYGKTEKLLLSDVKIPEIGDYEVLVEIHAASINQLDLKIKNGDFKLILHYQLP
CCEEEECCCCCCCCEEEECCCCCCCCCEEEEEEEEECCCEEEEEEEECCCEEEEEEEECC
LILGHDIAGIVVETGSKVSRFKIGDQIYGRAADFHIGSFAEYIAVNENDLAVKPINISME
EEECCCCEEEEEECCCCEEEEECCHHHHCCCCCCCCCCCEEEEEECCCCEEEEEECCCHH
QAASIPLVGLTVWQAFVEKAKLRKGQKVFIQAGSGGVGTIAIQFAKHLGAFVATTASEKN
HHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEHHHHHHHHHHHHHEEECCCCCC
FDMLKALGADIIIDYKKDNFENILKDYDVVLNSQDVKTLEKSLTILKPGGSIISISGPPT
HHHHHHCCCCEEEEECCCCHHHHHHHHHHEECCCHHHHHHHCEEEECCCCCEEEECCCCC
PEFAKSIGASAIIRFIIWLTSLKIRNRAKNLNINYSFLFMKANGRQLEQINNLLESKAIK
HHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHCHH
PVVDKVFSFEQTNDAVEYMEKGHAKGKVVVKIK
HHHHHHHCCCCCCHHHHHHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.26 {1,4-naphthoquinone}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA