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The map label for this gene is topB [H]

Identifier: 146300770

GI number: 146300770

Start: 3573959

End: 3576028

Strand: Reverse

Name: topB [H]

Synonym: Fjoh_3021

Alternate gene names: 146300770

Gene position: 3576028-3573959 (Counterclockwise)

Preceding gene: 146300771

Following gene: 146300769

Centisome position: 58.65

GC content: 50.43

Gene sequence:

>2070_bases
ATGAAAGTAGTTATTGCAGAGAAACCAAGTGCTGCAAGGGAGATAGCCGTACTGCTGGGAGCCAACGAAAAAAAGGATGG
GTACCTGGCAGGAAACGGCTATTTTGTTACTTGGGCTTTAGGACATCTGGTGGGTTTGGGCCTGCCGGAGGATTACGGCA
TAAAGGGCTTTGAGAAAAGCTCCCTGCCGATACTTCCGGATCCCTTTTTGCTTACGGTACGAAAACAAAAAATAGATGCC
GGTTACACTGCTGATGCAGGTGCGCAGAAACAGCTCAAAACCATCAAATATGTTGTAGGTCTGAGCGACCTGATCATAGT
GGCCACCGATGCAGGTCGCGAGGGGGAGCTGATTTTCCGCTACATCTATGAATATCTTGAGTTCAGGAAGCCTTTTGTCC
GCTTGTGGATCAGCAACCTCACCGAGAAAGCCATCAGGAAAGGATTGGAGAATCTGAAGCCAGCCTCTGAACTTGACGGA
CTGTACCTCGCGGCGCAGGCAAGGAGCAGGGCGGACTGGCTGGTAGGGATCAATGCCTCGCAGGCCCTGAGTCTTGCCCA
GGGAGACGGAGTCTTTTCCCTCGGAAGGGTACAGACACCGACGCTGGCGCTCGTATGTTCACGCTACCTGGAAAACAAAT
CCTTCAACATCGAGCCCTACTGGCAGCTCCAGCTGACGCACAGCAGGAATTTTATGCTCTTCACAAGCATCAGCGAGAAG
AGATGGGACAATAAAAGGCAGGCAATGGACGCACTGAGGGCGATCGAAAGAAATGCCGCGACGGCGTGGGTATCATCTGT
AGAGATCAAAAACTCAACAGAACAGCCGCCTCTGCTTTTTGACCTGACTGGACTGCAGAAACAGGCCAGTACGGACCTCA
GCCTTTCGGCCGGTGAAACGCTAAGCATTGCGCAGAGCCTTTATGAAAAGCAATTCATCAGCTATCCGCGAACCGGCAGC
AGGAGTATTTCCGCGGATGTATGGGATGAGATTCCCAGCCTTATAAAAGCATTGGGCACCCGTGAAAATTTCAGGGAGGC
TATATCCACATTGAAACTGGGGCGCCTGAACAAAAGGATTGTCAGCGAAGCGGGAGTTACCGACCACCACGGCCTGCTGC
CGACGGAACGAATCCCATCGGCGCTGTCCGGGAAGGAGACGGCGATCTACAACCTTATTGCCGGAAGGCTTCTTGAAGCA
GTGTCCGAACCGTGCATCAAGGAGATTACCAAGGTAAACCTGCAGGTGCTGCATTTTGATTTCATCCTCTCAGGGAGCAG
GATAATCGAATCTGGTTGGCGGGGTGTAAAAGCCCGCTTTTTGGAAAGTGGTGAGCCGGGACAGGAACTCCCTGAAATCA
AAAGGGGCGATGAGCTTAAAATCAAAGAGTCCGCGGTATTGGAGAAGGCAACAAGTCCGCCTGCCCTTTATACCGAGGCA
GGGCTTTTGAGTGTAATGGAAAATGCCGGAAAAAAGTCCCAGGACCTGCAAGAACGGAAGATCCTTCAGGCCATTGGCAT
TGGAACGCCTGCAACCAGGGCGGCGATTATCGAAACATTGTTTGACCGCAAGTATATCCAAAGACAGGGCAAATCATTAA
TCCCAACGGAAAAGGGGCTTATGGTGTATGAGCTGGTGAAGGATAAAAGGATTGCCGATGTGGCGATGACGGCCCAATGG
GAAACAGAATTGTGGAAGATTGAAAACAAAGAGGCTGATGCGGCGACATTCCAAAAGCAGATGGAATCCTATGCCACATC
CATAACAAAAGAGCTGCTGGAAACCCCACGTCCGCATGATATGGTGCCCGATCTCTGCTGCCCCAGATGCAACATAAATA
GGCTGCTTATTACAGAAAATATGGTCAAATGCCCTGAAAAGGAATGCAGGTGGGTTCAGTTCCGAAATGTATGCGGCGTA
CAGATCAGTCTGGCTGATATCGAAATACTGCTCCGTACTGGGAGAACCCCGCTGCTCAAGGGAATGAAAAGCAGGGCTGG
AAAATCATTTGATGCCTATATCGTGATGAATTACAAGGCTGAGACTTCTTTTGAATTTGCAAAAAAGTAA

Upstream 100 bases:

>100_bases
GGAACCCTTAAAATCCGGCCAGTTGGGTCCTGACACCCAGAAACAGAAAGAAGAGCAGCAGCCTGAAACCAAGTCGAAGG
GACGCAAAATGTAGCATGTC

Downstream 100 bases:

>100_bases
AGGCTGAAATGAGAATCTTTTCCTAACAACATCTTTTGACTTTATAAAGAAACCAATCCAGCATGACTATGAATTTTGAA
AAACTAAGCCTAAGGATACA

Product: DNA topoisomerase

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 689; Mature: 689

Protein sequence:

>689_residues
MKVVIAEKPSAAREIAVLLGANEKKDGYLAGNGYFVTWALGHLVGLGLPEDYGIKGFEKSSLPILPDPFLLTVRKQKIDA
GYTADAGAQKQLKTIKYVVGLSDLIIVATDAGREGELIFRYIYEYLEFRKPFVRLWISNLTEKAIRKGLENLKPASELDG
LYLAAQARSRADWLVGINASQALSLAQGDGVFSLGRVQTPTLALVCSRYLENKSFNIEPYWQLQLTHSRNFMLFTSISEK
RWDNKRQAMDALRAIERNAATAWVSSVEIKNSTEQPPLLFDLTGLQKQASTDLSLSAGETLSIAQSLYEKQFISYPRTGS
RSISADVWDEIPSLIKALGTRENFREAISTLKLGRLNKRIVSEAGVTDHHGLLPTERIPSALSGKETAIYNLIAGRLLEA
VSEPCIKEITKVNLQVLHFDFILSGSRIIESGWRGVKARFLESGEPGQELPEIKRGDELKIKESAVLEKATSPPALYTEA
GLLSVMENAGKKSQDLQERKILQAIGIGTPATRAAIIETLFDRKYIQRQGKSLIPTEKGLMVYELVKDKRIADVAMTAQW
ETELWKIENKEADAATFQKQMESYATSITKELLETPRPHDMVPDLCCPRCNINRLLITENMVKCPEKECRWVQFRNVCGV
QISLADIEILLRTGRTPLLKGMKSRAGKSFDAYIVMNYKAETSFEFAKK

Sequences:

>Translated_689_residues
MKVVIAEKPSAAREIAVLLGANEKKDGYLAGNGYFVTWALGHLVGLGLPEDYGIKGFEKSSLPILPDPFLLTVRKQKIDA
GYTADAGAQKQLKTIKYVVGLSDLIIVATDAGREGELIFRYIYEYLEFRKPFVRLWISNLTEKAIRKGLENLKPASELDG
LYLAAQARSRADWLVGINASQALSLAQGDGVFSLGRVQTPTLALVCSRYLENKSFNIEPYWQLQLTHSRNFMLFTSISEK
RWDNKRQAMDALRAIERNAATAWVSSVEIKNSTEQPPLLFDLTGLQKQASTDLSLSAGETLSIAQSLYEKQFISYPRTGS
RSISADVWDEIPSLIKALGTRENFREAISTLKLGRLNKRIVSEAGVTDHHGLLPTERIPSALSGKETAIYNLIAGRLLEA
VSEPCIKEITKVNLQVLHFDFILSGSRIIESGWRGVKARFLESGEPGQELPEIKRGDELKIKESAVLEKATSPPALYTEA
GLLSVMENAGKKSQDLQERKILQAIGIGTPATRAAIIETLFDRKYIQRQGKSLIPTEKGLMVYELVKDKRIADVAMTAQW
ETELWKIENKEADAATFQKQMESYATSITKELLETPRPHDMVPDLCCPRCNINRLLITENMVKCPEKECRWVQFRNVCGV
QISLADIEILLRTGRTPLLKGMKSRAGKSFDAYIVMNYKAETSFEFAKK
>Mature_689_residues
MKVVIAEKPSAAREIAVLLGANEKKDGYLAGNGYFVTWALGHLVGLGLPEDYGIKGFEKSSLPILPDPFLLTVRKQKIDA
GYTADAGAQKQLKTIKYVVGLSDLIIVATDAGREGELIFRYIYEYLEFRKPFVRLWISNLTEKAIRKGLENLKPASELDG
LYLAAQARSRADWLVGINASQALSLAQGDGVFSLGRVQTPTLALVCSRYLENKSFNIEPYWQLQLTHSRNFMLFTSISEK
RWDNKRQAMDALRAIERNAATAWVSSVEIKNSTEQPPLLFDLTGLQKQASTDLSLSAGETLSIAQSLYEKQFISYPRTGS
RSISADVWDEIPSLIKALGTRENFREAISTLKLGRLNKRIVSEAGVTDHHGLLPTERIPSALSGKETAIYNLIAGRLLEA
VSEPCIKEITKVNLQVLHFDFILSGSRIIESGWRGVKARFLESGEPGQELPEIKRGDELKIKESAVLEKATSPPALYTEA
GLLSVMENAGKKSQDLQERKILQAIGIGTPATRAAIIETLFDRKYIQRQGKSLIPTEKGLMVYELVKDKRIADVAMTAQW
ETELWKIENKEADAATFQKQMESYATSITKELLETPRPHDMVPDLCCPRCNINRLLITENMVKCPEKECRWVQFRNVCGV
QISLADIEILLRTGRTPLLKGMKSRAGKSFDAYIVMNYKAETSFEFAKK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=687, Percent_Identity=24.4541484716157, Blast_Score=110, Evalue=7e-24,
Organism=Homo sapiens, GI4507635, Length=588, Percent_Identity=24.1496598639456, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1788061, Length=640, Percent_Identity=31.5625, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI1787529, Length=651, Percent_Identity=25.9600614439324, Blast_Score=144, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI32563869, Length=619, Percent_Identity=25.0403877221325, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17555378, Length=481, Percent_Identity=29.5218295218295, Blast_Score=124, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323263, Length=622, Percent_Identity=24.5980707395498, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24585251, Length=570, Percent_Identity=26.8421052631579, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24640096, Length=581, Percent_Identity=23.407917383821, Blast_Score=110, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 76948; Mature: 76948

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVIAEKPSAAREIAVLLGANEKKDGYLAGNGYFVTWALGHLVGLGLPEDYGIKGFEKS
CEEEEECCCCHHHHEEEEECCCCCCCCEEECCCEEEEEEHHHHHHCCCCCCCCCCCCCCC
SLPILPDPFLLTVRKQKIDAGYTADAGAQKQLKTIKYVVGLSDLIIVATDAGREGELIFR
CCCCCCCCCEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHH
YIYEYLEFRKPFVRLWISNLTEKAIRKGLENLKPASELDGLYLAAQARSRADWLVGINAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCEEEEEHHCCCCCEEEECCHH
QALSLAQGDGVFSLGRVQTPTLALVCSRYLENKSFNIEPYWQLQLTHSRNFMLFTSISEK
HHHHHHCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCEEEEECCHHH
RWDNKRQAMDALRAIERNAATAWVSSVEIKNSTEQPPLLFDLTGLQKQASTDLSLSAGET
HCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEEECCCHHHCCCCCCCCCCCH
LSIAQSLYEKQFISYPRTGSRSISADVWDEIPSLIKALGTRENFREAISTLKLGRLNKRI
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VSEAGVTDHHGLLPTERIPSALSGKETAIYNLIAGRLLEAVSEPCIKEITKVNLQVLHFD
HHHCCCCCCCCCCCHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
FILSGSRIIESGWRGVKARFLESGEPGQELPEIKRGDELKIKESAVLEKATSPPALYTEA
EHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCEEEHHHHHHHHCCCCCCHHHHH
GLLSVMENAGKKSQDLQERKILQAIGIGTPATRAAIIETLFDRKYIQRQGKSLIPTEKGL
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
MVYELVKDKRIADVAMTAQWETELWKIENKEADAATFQKQMESYATSITKELLETPRPHD
CHHHHHHCCHHHHHHEECCHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
MVPDLCCPRCNINRLLITENMVKCPEKECRWVQFRNVCGVQISLADIEILLRTGRTPLLK
CCCHHCCCCCCCCEEEEECHHHHCCHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCHHHH
GMKSRAGKSFDAYIVMNYKAETSFEFAKK
HHHHHCCCCCEEEEEEEECCCCCHHHHCC
>Mature Secondary Structure
MKVVIAEKPSAAREIAVLLGANEKKDGYLAGNGYFVTWALGHLVGLGLPEDYGIKGFEKS
CEEEEECCCCHHHHEEEEECCCCCCCCEEECCCEEEEEEHHHHHHCCCCCCCCCCCCCCC
SLPILPDPFLLTVRKQKIDAGYTADAGAQKQLKTIKYVVGLSDLIIVATDAGREGELIFR
CCCCCCCCCEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHH
YIYEYLEFRKPFVRLWISNLTEKAIRKGLENLKPASELDGLYLAAQARSRADWLVGINAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCEEEEEHHCCCCCEEEECCHH
QALSLAQGDGVFSLGRVQTPTLALVCSRYLENKSFNIEPYWQLQLTHSRNFMLFTSISEK
HHHHHHCCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCEEEEECCHHH
RWDNKRQAMDALRAIERNAATAWVSSVEIKNSTEQPPLLFDLTGLQKQASTDLSLSAGET
HCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEEECCCHHHCCCCCCCCCCCH
LSIAQSLYEKQFISYPRTGSRSISADVWDEIPSLIKALGTRENFREAISTLKLGRLNKRI
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VSEAGVTDHHGLLPTERIPSALSGKETAIYNLIAGRLLEAVSEPCIKEITKVNLQVLHFD
HHHCCCCCCCCCCCHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
FILSGSRIIESGWRGVKARFLESGEPGQELPEIKRGDELKIKESAVLEKATSPPALYTEA
EHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCEEEHHHHHHHHCCCCCCHHHHH
GLLSVMENAGKKSQDLQERKILQAIGIGTPATRAAIIETLFDRKYIQRQGKSLIPTEKGL
HHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
MVYELVKDKRIADVAMTAQWETELWKIENKEADAATFQKQMESYATSITKELLETPRPHD
CHHHHHHCCHHHHHHEECCHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
MVPDLCCPRCNINRLLITENMVKCPEKECRWVQFRNVCGVQISLADIEILLRTGRTPLLK
CCCHHCCCCCCCCEEEEECHHHHCCHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCHHHH
GMKSRAGKSFDAYIVMNYKAETSFEFAKK
HHHHHCCCCCEEEEEEEECCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA