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The map label for this gene is pelE [H]

Identifier: 146300321

GI number: 146300321

Start: 3052291

End: 3053397

Strand: Reverse

Name: pelE [H]

Synonym: Fjoh_2566

Alternate gene names: 146300321

Gene position: 3053397-3052291 (Counterclockwise)

Preceding gene: 146300323

Following gene: 146300317

Centisome position: 50.08

GC content: 39.39

Gene sequence:

>1107_bases
ATGAACTTAAAATTAAAACTTTCAATGTTTTTACTAACATTGACTTTCGGATTTACAGCATGTAACTCTGAAGAAATTGC
TTCGAACACAGAAGCAAAAACTGAAGCAGTAACCGAAACTCCTTCAGGCAATTTAACTGCTAAAATTGGAAACTGTGCCG
AAGTTCCGGGCTGGGCTTCTCAAAACGGCGGAACAACCGGCGGAGGTTCTGCTGCTGAAACTACGGTTACTACTTATGCT
CAGCTGAAATCGGCTATTGAAAACAGCTCTGTAAAAGTTATTAAAATTTCAGGAACTATTACAGTTACGACCAGATTGTC
ATTTCAGGATCAAACCGGTAAAACTATTTATGGTGCAAACGGTGCAAAGTTGGTTTCGACTAATCAGACAAAAGATGCTT
CTGGAATCATCAACATCAAAAGATGTAAAAACATTATCATTAGAAACCTAATTTTTGAAGGCCCGGGTGCTTATGATACT
GACGGATGGGACAATGCAATTTTGGACGAATGTACAAATGTATGGGTTGACCACTGCGAATTTAGAGATGGTGTTGATGG
AAACTTCGACATTAAAAACAAATCTGATTATATTTCGGTTACGTATTCTAAATTCCATTATTTAAAACCGCCAAAAGCTG
GAGGTTCTGGAGGATCTGATGATCACAGATATTCAAACTTAATTGGTTCAAGCGACGGTGCAACAGGCGACCGCGGAAAA
TTGAGAATCACTTTTGCAAGATGCTGGTGGGCTCCTGGATGTAAAGAAAGAATGCCGAGAGTTCGTTTTGGGAAAGTACA
TATCGTAAACAGTTTCTTTAACAGTACGGTAAGCAACAAATGCATCGCTGCAGGTTTTGAAGCTGATATTCGCGTAGAAA
GCAATGTTTTTGAAGGTGTAAAAACACCAATCGATTTAATGACAGGTTATACTGCAGTTACAGCAACCGACAACGTATTT
ACAAGTGTATCAGGAAATCAGGCTGGAAGCGGTACAGCTTTTACACCGCCTTATTCTATTGTAAAGCTTAATAAAACAGC
TGTAAAAGCCGATATTTCTGCTAACGCAGGAGCAACTTTGGGCGGTAACATTTGCGGCTCTTTCTAA

Upstream 100 bases:

>100_bases
TACAAGATTTTTTTACAAAAGTTTGAACTTGTTCAATTTTTTTTCTACAGATAATTCTGAGTTTTGCGTTGTTAAACTTT
TATAAATAACCAAACTTATT

Downstream 100 bases:

>100_bases
TAAACTCTGGATAGCAAAAAAATGAGTTAGTTTTAGTTTCTAAAACCACGGATTGCAGTAAGGCAGTTCGTGGTTTTTTA
TTTTTTTTACTAAGACACAA

Product: Pectate lyase/Amb allergen

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MNLKLKLSMFLLTLTFGFTACNSEEIASNTEAKTEAVTETPSGNLTAKIGNCAEVPGWASQNGGTTGGGSAAETTVTTYA
QLKSAIENSSVKVIKISGTITVTTRLSFQDQTGKTIYGANGAKLVSTNQTKDASGIINIKRCKNIIIRNLIFEGPGAYDT
DGWDNAILDECTNVWVDHCEFRDGVDGNFDIKNKSDYISVTYSKFHYLKPPKAGGSGGSDDHRYSNLIGSSDGATGDRGK
LRITFARCWWAPGCKERMPRVRFGKVHIVNSFFNSTVSNKCIAAGFEADIRVESNVFEGVKTPIDLMTGYTAVTATDNVF
TSVSGNQAGSGTAFTPPYSIVKLNKTAVKADISANAGATLGGNICGSF

Sequences:

>Translated_368_residues
MNLKLKLSMFLLTLTFGFTACNSEEIASNTEAKTEAVTETPSGNLTAKIGNCAEVPGWASQNGGTTGGGSAAETTVTTYA
QLKSAIENSSVKVIKISGTITVTTRLSFQDQTGKTIYGANGAKLVSTNQTKDASGIINIKRCKNIIIRNLIFEGPGAYDT
DGWDNAILDECTNVWVDHCEFRDGVDGNFDIKNKSDYISVTYSKFHYLKPPKAGGSGGSDDHRYSNLIGSSDGATGDRGK
LRITFARCWWAPGCKERMPRVRFGKVHIVNSFFNSTVSNKCIAAGFEADIRVESNVFEGVKTPIDLMTGYTAVTATDNVF
TSVSGNQAGSGTAFTPPYSIVKLNKTAVKADISANAGATLGGNICGSF
>Mature_368_residues
MNLKLKLSMFLLTLTFGFTACNSEEIASNTEAKTEAVTETPSGNLTAKIGNCAEVPGWASQNGGTTGGGSAAETTVTTYA
QLKSAIENSSVKVIKISGTITVTTRLSFQDQTGKTIYGANGAKLVSTNQTKDASGIINIKRCKNIIIRNLIFEGPGAYDT
DGWDNAILDECTNVWVDHCEFRDGVDGNFDIKNKSDYISVTYSKFHYLKPPKAGGSGGSDDHRYSNLIGSSDGATGDRGK
LRITFARCWWAPGCKERMPRVRFGKVHIVNSFFNSTVSNKCIAAGFEADIRVESNVFEGVKTPIDLMTGYTAVTATDNVF
TSVSGNQAGSGTAFTPPYSIVKLNKTAVKADISANAGATLGGNICGSF

Specific function: Involved in maceration and soft-rotting of plant tissue [H]

COG id: COG3866

COG function: function code G; Pectate lyase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide lyase 1 family. PLBC subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002022
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00544 Pec_lyase_C [H]

EC number: =4.2.2.2 [H]

Molecular weight: Translated: 39130; Mature: 39130

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKLKLSMFLLTLTFGFTACNSEEIASNTEAKTEAVTETPSGNLTAKIGNCAEVPGWAS
CCEEEEHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHCCCCCCCEEEEECCCCCCCCCCC
QNGGTTGGGSAAETTVTTYAQLKSAIENSSVKVIKISGTITVTTRLSFQDQTGKTIYGAN
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECEEEEEEEEEECCCCCCEEECCC
GAKLVSTNQTKDASGIINIKRCKNIIIRNLIFEGPGAYDTDGWDNAILDECTNVWVDHCE
CCEEEECCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
FRDGVDGNFDIKNKSDYISVTYSKFHYLKPPKAGGSGGSDDHRYSNLIGSSDGATGDRGK
CCCCCCCCEEECCCCCEEEEEEEEEEEECCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCE
LRITFARCWWAPGCKERMPRVRFGKVHIVNSFFNSTVSNKCIAAGFEADIRVESNVFEGV
EEEEEEEEECCCCHHHHCCCCEECEEEEEHHHHHHHHCCCEEEECCCCEEEECCHHHHCC
KTPIDLMTGYTAVTATDNVFTSVSGNQAGSGTAFTPPYSIVKLNKTAVKADISANAGATL
CCCHHHHCCCEEEEECCCEEEECCCCCCCCCCCCCCCCEEEEECCCEEEEECCCCCCCEE
GGNICGSF
CCCCCCCC
>Mature Secondary Structure
MNLKLKLSMFLLTLTFGFTACNSEEIASNTEAKTEAVTETPSGNLTAKIGNCAEVPGWAS
CCEEEEHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHCCCCCCCEEEEECCCCCCCCCCC
QNGGTTGGGSAAETTVTTYAQLKSAIENSSVKVIKISGTITVTTRLSFQDQTGKTIYGAN
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECEEEEEEEEEECCCCCCEEECCC
GAKLVSTNQTKDASGIINIKRCKNIIIRNLIFEGPGAYDTDGWDNAILDECTNVWVDHCE
CCEEEECCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
FRDGVDGNFDIKNKSDYISVTYSKFHYLKPPKAGGSGGSDDHRYSNLIGSSDGATGDRGK
CCCCCCCCEEECCCCCEEEEEEEEEEEECCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCE
LRITFARCWWAPGCKERMPRVRFGKVHIVNSFFNSTVSNKCIAAGFEADIRVESNVFEGV
EEEEEEEEECCCCHHHHCCCCEECEEEEEHHHHHHHHCCCEEEECCCCEEEECCHHHHCC
KTPIDLMTGYTAVTATDNVFTSVSGNQAGSGTAFTPPYSIVKLNKTAVKADISANAGATL
CCCHHHHCCCEEEEECCCEEEECCCCCCCCCCCCCCCCEEEEECCCEEEEECCCCCCCEE
GGNICGSF
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536853; 8081738 [H]