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The map label for this gene is hemN [H]

Identifier: 146300297

GI number: 146300297

Start: 3027577

End: 3028941

Strand: Reverse

Name: hemN [H]

Synonym: Fjoh_2542

Alternate gene names: 146300297

Gene position: 3028941-3027577 (Counterclockwise)

Preceding gene: 146300298

Following gene: 146300290

Centisome position: 49.68

GC content: 34.21

Gene sequence:

>1365_bases
ATGAAAATTTCACTAACCCAAAAGTACAATGTACCAGGGCCAAGATACACCAGTTATCCTACCGTACCTTATTGGAATGA
AGCTGCTTTTACGATAGAAGAATGGAAATCATCATTTCAGAGAGCGTTTTCTGAAAGCAATTCTCAAAACGGAATAAGTT
TATACATTCATCTTCCATTTTGCGAAAGCATGTGCACCTTTTGCGGCTGCAACAAACGAATTACAAAGAATCATACTGTT
GAAGAAAAATACATACAAGCCGTTTTAAAAGAATGGAGATTGTACTGCAAATTATTACCAGAAAGACCTCTTATAAAGGA
AATTCATTTAGGAGGCGGAACACCTACTTTTTTTTCAGCACATAATTTAGAGCATTTAATAAATGGTATTTTCAGCTTTG
CCAATAAAGCCGAAAATCATGAGTTTAGTTTTGAAGGGCATCCCAATAACACTACTTATGATCAATTAAAAAGATTATAT
GATTTAGGCTTTCGCCGGGTTAGTTTTGGAGTTCAGGATTATTCTGAAAAAGTACAGAAGGCAATCAACCGAATTCAGCC
TTTTCACAATGTAGCCAAAGTAACTTTTTGGGCCAAAGAAATTGGTTACACTTCTATAGGACACGATATTATATTTGGGC
TTCCTTTTCAAACGGTTGAAGATGTAATTGATACAGTCGAAAAAACCAATTCTGTAAAACCAGACAGACTGGCATTTTAC
AGTTATGCTCACGTACCCTGGATAAAAGGAAACGGACAACGCGGTTTTAATGAAGAAAACCTCCCAAAAGATGGCGAGAA
AAGAAAACTTTACGAAACTGGTAAACTATTACTTTCTGCAAATGGGTATCATGAAATTGGCATGGATCATTTTGCTTTAA
AGTCTGACAGTTTATATCACTCGGCACAAAACAAAACAATGCATCGAAATTTTATGGGTTACAGTGCTTCTAAAACACAG
CTTATGATTGGTTTAGGAGTTTCTTCAATTAGCGACAGCTGGTATGGCTTTGCTCAAAATACTAAAAACCTGGAAGATTA
CTATCAAATTTTAGAATGGAACCAGCTTCCGGTTGTAAAAGGTCATATTTTAAGTGATGAAGATCTTATCATTAGAAAAC
ATATTTTAAACTTAACCTGTGAGCTTGAAACATCATGGAGCAATGATTCTTTATATTTTAAAGAACTGCCTGAAGTTTTA
TTATCTCTAAAAGAAATAGAAAGCGATCAATTGATTTTAATTGAAGAGAATAAAATAAAAATTACTGAAGCCGGAAGACC
ATTTGTTCGTAATATCTGTATGGCTTTTGATTTACATTTAAAAAGAAAGTCACCGGAAACCAATTTGTTTTCGATGACGG
TTTAA

Upstream 100 bases:

>100_bases
GCTCTTTATGGGTCTCTTTTTTTTTGTTAATTAATATGATATTTGTCATTTTATACCCCGGTTTCGTGTAGTAATTTTGG
TGAAAACTAAATCCGTCTTT

Downstream 100 bases:

>100_bases
TGACAATTAGGAGTTCCCTGAACAAACAAACTCATATTTGAAGGAGATAAATAGGGAATTCCTAAACCTAAACCTCTCAG
AATAAACAAAATTCCAATAA

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MKISLTQKYNVPGPRYTSYPTVPYWNEAAFTIEEWKSSFQRAFSESNSQNGISLYIHLPFCESMCTFCGCNKRITKNHTV
EEKYIQAVLKEWRLYCKLLPERPLIKEIHLGGGTPTFFSAHNLEHLINGIFSFANKAENHEFSFEGHPNNTTYDQLKRLY
DLGFRRVSFGVQDYSEKVQKAINRIQPFHNVAKVTFWAKEIGYTSIGHDIIFGLPFQTVEDVIDTVEKTNSVKPDRLAFY
SYAHVPWIKGNGQRGFNEENLPKDGEKRKLYETGKLLLSANGYHEIGMDHFALKSDSLYHSAQNKTMHRNFMGYSASKTQ
LMIGLGVSSISDSWYGFAQNTKNLEDYYQILEWNQLPVVKGHILSDEDLIIRKHILNLTCELETSWSNDSLYFKELPEVL
LSLKEIESDQLILIEENKIKITEAGRPFVRNICMAFDLHLKRKSPETNLFSMTV

Sequences:

>Translated_454_residues
MKISLTQKYNVPGPRYTSYPTVPYWNEAAFTIEEWKSSFQRAFSESNSQNGISLYIHLPFCESMCTFCGCNKRITKNHTV
EEKYIQAVLKEWRLYCKLLPERPLIKEIHLGGGTPTFFSAHNLEHLINGIFSFANKAENHEFSFEGHPNNTTYDQLKRLY
DLGFRRVSFGVQDYSEKVQKAINRIQPFHNVAKVTFWAKEIGYTSIGHDIIFGLPFQTVEDVIDTVEKTNSVKPDRLAFY
SYAHVPWIKGNGQRGFNEENLPKDGEKRKLYETGKLLLSANGYHEIGMDHFALKSDSLYHSAQNKTMHRNFMGYSASKTQ
LMIGLGVSSISDSWYGFAQNTKNLEDYYQILEWNQLPVVKGHILSDEDLIIRKHILNLTCELETSWSNDSLYFKELPEVL
LSLKEIESDQLILIEENKIKITEAGRPFVRNICMAFDLHLKRKSPETNLFSMTV
>Mature_454_residues
MKISLTQKYNVPGPRYTSYPTVPYWNEAAFTIEEWKSSFQRAFSESNSQNGISLYIHLPFCESMCTFCGCNKRITKNHTV
EEKYIQAVLKEWRLYCKLLPERPLIKEIHLGGGTPTFFSAHNLEHLINGIFSFANKAENHEFSFEGHPNNTTYDQLKRLY
DLGFRRVSFGVQDYSEKVQKAINRIQPFHNVAKVTFWAKEIGYTSIGHDIIFGLPFQTVEDVIDTVEKTNSVKPDRLAFY
SYAHVPWIKGNGQRGFNEENLPKDGEKRKLYETGKLLLSANGYHEIGMDHFALKSDSLYHSAQNKTMHRNFMGYSASKTQ
LMIGLGVSSISDSWYGFAQNTKNLEDYYQILEWNQLPVVKGHILSDEDLIIRKHILNLTCELETSWSNDSLYFKELPEVL
LSLKEIESDQLILIEENKIKITEAGRPFVRNICMAFDLHLKRKSPETNLFSMTV

Specific function: Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX [H]

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=242, Percent_Identity=23.1404958677686, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI87082341, Length=453, Percent_Identity=35.3200883002208, Blast_Score=274, Evalue=9e-75,
Organism=Escherichia coli, GI1789325, Length=241, Percent_Identity=27.8008298755187, Blast_Score=113, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004558
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: =1.3.99.22 [H]

Molecular weight: Translated: 52468; Mature: 52468

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISLTQKYNVPGPRYTSYPTVPYWNEAAFTIEEWKSSFQRAFSESNSQNGISLYIHLPF
CEEEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCEEEEEECCC
CESMCTFCGCNKRITKNHTVEEKYIQAVLKEWRLYCKLLPERPLIKEIHLGGGTPTFFSA
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHEEECCCCCCCCCCC
HNLEHLINGIFSFANKAENHEFSFEGHPNNTTYDQLKRLYDLGFRRVSFGVQDYSEKVQK
CCHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCHHHHHCCHHHHHHHHHH
AINRIQPFHNVAKVTFWAKEIGYTSIGHDIIFGLPFQTVEDVIDTVEKTNSVKPDRLAFY
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEECCCHHHHHHHHHHHHHHCCCCCCCEEEE
SYAHVPWIKGNGQRGFNEENLPKDGEKRKLYETGKLLLSANGYHEIGMDHFALKSDSLYH
EEECCCEEECCCCCCCCCCCCCCCCCHHHHHHCCCEEEECCCCHHCCCCHHEECCCCHHH
SAQNKTMHRNFMGYSASKTQLMIGLGVSSISDSWYGFAQNTKNLEDYYQILEWNQLPVVK
HHHCCHHHHHHCCCCCCCCEEEEEECHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEE
GHILSDEDLIIRKHILNLTCELETSWSNDSLYFKELPEVLLSLKEIESDQLILIEENKIK
CEEECCCCHHHHHHHHCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCEEE
ITEAGRPFVRNICMAFDLHLKRKSPETNLFSMTV
EEECCCHHHHHHHHHHHHHEECCCCCCCEEEECC
>Mature Secondary Structure
MKISLTQKYNVPGPRYTSYPTVPYWNEAAFTIEEWKSSFQRAFSESNSQNGISLYIHLPF
CEEEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCEEEEEECCC
CESMCTFCGCNKRITKNHTVEEKYIQAVLKEWRLYCKLLPERPLIKEIHLGGGTPTFFSA
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHEEECCCCCCCCCCC
HNLEHLINGIFSFANKAENHEFSFEGHPNNTTYDQLKRLYDLGFRRVSFGVQDYSEKVQK
CCHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCHHHHHCCHHHHHHHHHH
AINRIQPFHNVAKVTFWAKEIGYTSIGHDIIFGLPFQTVEDVIDTVEKTNSVKPDRLAFY
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEECCCHHHHHHHHHHHHHHCCCCCCCEEEE
SYAHVPWIKGNGQRGFNEENLPKDGEKRKLYETGKLLLSANGYHEIGMDHFALKSDSLYH
EEECCCEEECCCCCCCCCCCCCCCCCHHHHHHCCCEEEECCCCHHCCCCHHEECCCCHHH
SAQNKTMHRNFMGYSASKTQLMIGLGVSSISDSWYGFAQNTKNLEDYYQILEWNQLPVVK
HHHCCHHHHHHCCCCCCCCEEEEEECHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEE
GHILSDEDLIIRKHILNLTCELETSWSNDSLYFKELPEVLLSLKEIESDQLILIEENKIK
CEEECCCCHHHHHHHHCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCEEE
ITEAGRPFVRNICMAFDLHLKRKSPETNLFSMTV
EEECCCHHHHHHHHHHHHHEECCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]